FI121075B - Geneettisesti muokattu sienisolu, menetelmä rekombinanttituotteiden tuottamiseksi sekä DNA-molekyyli ja DNA-fragmentti ja näiden käyttö - Google Patents
Geneettisesti muokattu sienisolu, menetelmä rekombinanttituotteiden tuottamiseksi sekä DNA-molekyyli ja DNA-fragmentti ja näiden käyttö Download PDFInfo
- Publication number
- FI121075B FI121075B FI950628A FI950628A FI121075B FI 121075 B FI121075 B FI 121075B FI 950628 A FI950628 A FI 950628A FI 950628 A FI950628 A FI 950628A FI 121075 B FI121075 B FI 121075B
- Authority
- FI
- Finland
- Prior art keywords
- fungal cell
- dna
- leu
- ser
- gene
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE4226971 | 1992-08-14 | ||
DE4226971A DE4226971C2 (de) | 1992-08-14 | 1992-08-14 | Modifizierte Pilzzellen und Verfahren zur Herstellung rekombinanter Produkte |
EP9302179 | 1993-08-16 | ||
PCT/EP1993/002179 WO1994004687A1 (en) | 1992-08-14 | 1993-08-16 | Modified fungal cells and method for producing recombinant products |
Publications (3)
Publication Number | Publication Date |
---|---|
FI950628A0 FI950628A0 (fi) | 1995-02-13 |
FI950628A FI950628A (fi) | 1995-02-13 |
FI121075B true FI121075B (fi) | 2010-06-30 |
Family
ID=6465579
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FI950628A FI121075B (fi) | 1992-08-14 | 1995-02-13 | Geneettisesti muokattu sienisolu, menetelmä rekombinanttituotteiden tuottamiseksi sekä DNA-molekyyli ja DNA-fragmentti ja näiden käyttö |
Country Status (13)
Country | Link |
---|---|
US (1) | US5714377A (es) |
EP (1) | EP0658203B1 (es) |
JP (1) | JP3630424B2 (es) |
AT (1) | ATE245701T1 (es) |
AU (1) | AU679935B2 (es) |
CA (1) | CA2140894C (es) |
DE (3) | DE4244915C2 (es) |
ES (1) | ES2203619T3 (es) |
FI (1) | FI121075B (es) |
NO (1) | NO320349B1 (es) |
NZ (1) | NZ255350A (es) |
WO (1) | WO1994004687A1 (es) |
ZA (1) | ZA935915B (es) |
Families Citing this family (56)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2686899B1 (fr) * | 1992-01-31 | 1995-09-01 | Rhone Poulenc Rorer Sa | Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant. |
US5728553A (en) | 1992-09-23 | 1998-03-17 | Delta Biotechnology Limited | High purity albumin and method of producing |
JPH08173170A (ja) * | 1994-05-25 | 1996-07-09 | Kirin Brewery Co Ltd | キャンディダ・ユティリス酵母の形質転換系、およびそれによる異種遺伝子の発現 |
DE69631127T2 (de) * | 1995-05-18 | 2004-07-08 | Genencor International, Inc., Palo Alto | Glykosyltransferase expression in aspergillus |
GB9526733D0 (en) * | 1995-12-30 | 1996-02-28 | Delta Biotechnology Ltd | Fusion proteins |
AU2007200859A1 (en) * | 1999-01-30 | 2007-03-22 | Novozymes Delta Limited | Process |
GB9902000D0 (en) | 1999-01-30 | 1999-03-17 | Delta Biotechnology Ltd | Process |
CA2405557C (en) * | 2000-04-12 | 2013-09-24 | Human Genome Sciences, Inc. | Albumin fusion proteins |
US6946134B1 (en) | 2000-04-12 | 2005-09-20 | Human Genome Sciences, Inc. | Albumin fusion proteins |
US20050100991A1 (en) * | 2001-04-12 | 2005-05-12 | Human Genome Sciences, Inc. | Albumin fusion proteins |
CN1592786A (zh) * | 2000-12-05 | 2005-03-09 | 宾夕法尼亚州立研究基金会 | 在酵母菌中高效生产异源性蛋白质的方法和组合物 |
US7507413B2 (en) | 2001-04-12 | 2009-03-24 | Human Genome Sciences, Inc. | Albumin fusion proteins |
US20060084794A1 (en) * | 2001-04-12 | 2006-04-20 | Human Genome Sciences, Inc. | Albumin fusion proteins |
US20050244931A1 (en) * | 2001-04-12 | 2005-11-03 | Human Genome Sciences, Inc. | Albumin fusion proteins |
US20050054051A1 (en) * | 2001-04-12 | 2005-03-10 | Human Genome Sciences, Inc. | Albumin fusion proteins |
CA2383556A1 (en) * | 2001-05-16 | 2002-11-16 | Pharmacia & Upjohn Company | High-efficiency assay for protein mannosyl transferases |
US7176278B2 (en) | 2001-08-30 | 2007-02-13 | Biorexis Technology, Inc. | Modified transferrin fusion proteins |
WO2003030821A2 (en) * | 2001-10-05 | 2003-04-17 | Human Genome Sciences, Inc. | Albumin fusion proteins |
AU2002364587A1 (en) * | 2001-12-21 | 2003-07-30 | Human Genome Sciences, Inc. | Albumin fusion proteins |
EP2261250B1 (en) * | 2001-12-21 | 2015-07-01 | Human Genome Sciences, Inc. | GCSF-Albumin fusion proteins |
NZ534492A (en) | 2002-02-07 | 2009-10-30 | Novozymes Delta Ltd | Albumin-fused kunitz domain peptides |
BR0315943A (pt) * | 2002-12-20 | 2005-09-13 | Unilever Nv | Método para a produção de uma proteìna anticongelante e composição |
GB0329722D0 (en) | 2003-12-23 | 2004-01-28 | Delta Biotechnology Ltd | Modified plasmid and use thereof |
GB0329681D0 (en) | 2003-12-23 | 2004-01-28 | Delta Biotechnology Ltd | Gene expression technique |
DK2330200T3 (en) | 2004-12-23 | 2017-07-24 | Albumedix As | GENE EXPRESSION TECHNIQUE |
JP5284789B2 (ja) * | 2005-11-15 | 2013-09-11 | グライコフィ, インコーポレイテッド | O−グリコシル化を減少している糖タンパク質の生成 |
AU2012203729B2 (en) * | 2005-11-22 | 2014-01-30 | Glycofi, Inc | Production of glycoproteins with reduced O-glycosylation |
EP1816201A1 (en) * | 2006-02-06 | 2007-08-08 | CSL Behring GmbH | Modified coagulation factor VIIa with extended half-life |
WO2007132949A1 (ja) | 2006-05-16 | 2007-11-22 | National Institute Of Advanced Industrial Science And Technology | タンパク質の高分泌生産方法 |
KR101193722B1 (ko) | 2006-07-24 | 2013-01-11 | 바이오렉시스 파마슈티칼 코포레이션 | 엑센딘 융합 단백질 |
GB0711424D0 (en) * | 2007-06-13 | 2007-07-25 | Novozymes Delta Ltd | Recombinant transferrin mutants |
US20110124576A1 (en) | 2007-08-08 | 2011-05-26 | Novozymes A/S | Transferrin Variants and Conjugates |
US8637435B2 (en) * | 2007-11-16 | 2014-01-28 | Merck Sharp & Dohme Corp. | Eukaryotic cell display systems |
CN101945998B (zh) | 2008-02-20 | 2013-09-18 | 格利科菲公司 | 用于蛋白生产的载体和酵母菌株 |
JP5731827B2 (ja) * | 2008-03-03 | 2015-06-10 | グライコフィ, インコーポレイテッド | 下等真核生物中での組換えタンパク質の表面ディスプレイ |
WO2010019487A1 (en) * | 2008-08-12 | 2010-02-18 | Glycofi, Inc. | Improved vectors and yeast strains for protein production: ca2+ atpase overexpression |
US9120871B2 (en) | 2009-01-23 | 2015-09-01 | Novo Nordisk A/S | Process for preparing FGF21 with low degree of O-glycosylation |
DK2393834T3 (da) | 2009-02-06 | 2013-06-24 | Novozymes Biopharma Dk As | Fremgangsmåde til oprensning |
EP2396347B1 (en) | 2009-02-11 | 2017-04-12 | Albumedix A/S | Albumin variants and conjugates |
KR20110122134A (ko) | 2009-02-25 | 2011-11-09 | 머크 샤프 앤드 돔 코포레이션 | 글리코공학처리된 효모 피키아 파스토리스에서 갈락토스 동화 경로의 대사 공학 |
AU2010311332B2 (en) | 2009-10-30 | 2015-04-23 | Albumedix Ltd. | Albumin variants |
US9528116B2 (en) | 2009-10-30 | 2016-12-27 | Merck Sharp & Dohme Corp. | Method for producing therapeutic proteins in Pichia pastoris lacking dipeptidyl aminopeptidase activity |
WO2011099028A1 (en) * | 2010-02-10 | 2011-08-18 | Biocon Limited | Method of reducing glycosylation of proteins, processes and proteins thereof |
KR101930961B1 (ko) | 2010-02-24 | 2018-12-19 | 머크 샤프 앤드 돔 코포레이션 | 피키아 파스토리스에서 생산된 치료 당단백질 상의 n-글리코실화 부위 점유를 증가시키는 방법 |
JP5969458B2 (ja) | 2010-04-09 | 2016-08-17 | アルブミディクス アクティーゼルスカブ | アルブミン誘導体及び変異体 |
NZ603883A (en) | 2010-05-27 | 2015-01-30 | Merck Sharp & Dohme | Method for preparing antibodies having improved properties |
JP5885191B2 (ja) | 2011-01-04 | 2016-03-15 | 国立研究開発法人産業技術総合研究所 | 糖鎖改変酵母及びそれを用いた糖タンパク質の製造方法 |
EP2678440B1 (en) | 2011-02-25 | 2018-05-23 | Merck Sharp & Dohme Corp. | Yeast strain for the production of proteins with modified o-glycosylation |
EP2714732A4 (en) | 2011-05-25 | 2014-12-10 | Merck Sharp & Dohme | PROCESS FOR PREPARING FC-CONTAINING POLYPEPTIDES WITH IMPROVED PROPERTIES |
WO2013075066A2 (en) | 2011-11-18 | 2013-05-23 | Eleven Biotherapeutics, Inc. | Proteins with improved half-life and other properties |
MX2014010278A (es) | 2012-03-16 | 2015-03-05 | Novozymes Biopharma Dk As | Variantes de albumina. |
MX2015005363A (es) | 2012-11-08 | 2015-11-06 | Novozymes Biopharma Dk As | Variantes de albumina. |
JP2016521701A (ja) | 2013-06-07 | 2016-07-25 | ノヴォ ノルディスク アー/エス | 成熟インスリンポリペプチドを作製するための方法 |
CA2916594C (en) | 2013-07-04 | 2020-03-10 | Novartis Ag | O-mannosyltransferase deficient filamentous fungal cells and methods of use thereof |
EP3172333B1 (en) | 2014-07-21 | 2020-05-13 | Glykos Finland Oy | Production of glycoproteins with mammalian-like n-glycans in filamentous fungi |
WO2017029407A1 (en) | 2015-08-20 | 2017-02-23 | Albumedix A/S | Albumin variants and conjugates |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2007676B (en) * | 1977-11-08 | 1982-09-08 | Genentech Inc | Method and means for microbial polypeptide expression |
IT1203758B (it) * | 1986-03-27 | 1989-02-23 | Univ Roma | Vettori di clonazione e di espressione di geni eterologhi in lieviti e lieviti trasformati con tali vettori |
US4929553A (en) * | 1987-05-29 | 1990-05-29 | Canadian Patents & Development Ltd. | Protease for specific processing of secreted proteins |
FR2649991B2 (fr) * | 1988-08-05 | 1994-03-04 | Rhone Poulenc Sante | Utilisation de derives stables du plasmide pkd1 pour l'expression et la secretion de proteines heterologues dans les levures du genre kluyveromyces |
FR2655660B1 (fr) * | 1989-12-11 | 1992-03-20 | Rhone Poulenc Sante | Nouveaux polypeptides, sequences d'adn permettant leur expression, procede de preparation et leur utilisation. |
-
1992
- 1992-08-14 DE DE4244915A patent/DE4244915C2/de not_active Expired - Lifetime
- 1992-08-14 DE DE4226971A patent/DE4226971C2/de not_active Expired - Lifetime
-
1993
- 1993-08-13 ZA ZA935915A patent/ZA935915B/xx unknown
- 1993-08-16 AT AT93919067T patent/ATE245701T1/de active
- 1993-08-16 WO PCT/EP1993/002179 patent/WO1994004687A1/en active IP Right Grant
- 1993-08-16 NZ NZ255350A patent/NZ255350A/en not_active IP Right Cessation
- 1993-08-16 ES ES93919067T patent/ES2203619T3/es not_active Expired - Lifetime
- 1993-08-16 EP EP93919067A patent/EP0658203B1/en not_active Expired - Lifetime
- 1993-08-16 JP JP50589294A patent/JP3630424B2/ja not_active Expired - Lifetime
- 1993-08-16 CA CA002140894A patent/CA2140894C/en not_active Expired - Lifetime
- 1993-08-16 DE DE69333112T patent/DE69333112T2/de not_active Expired - Lifetime
- 1993-08-16 US US08/381,931 patent/US5714377A/en not_active Expired - Lifetime
- 1993-08-16 AU AU49482/93A patent/AU679935B2/en not_active Expired
-
1995
- 1995-02-13 NO NO19950534A patent/NO320349B1/no not_active IP Right Cessation
- 1995-02-13 FI FI950628A patent/FI121075B/fi not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
AU679935B2 (en) | 1997-07-17 |
WO1994004687A1 (en) | 1994-03-03 |
AU4948293A (en) | 1994-03-15 |
FI950628A0 (fi) | 1995-02-13 |
ATE245701T1 (de) | 2003-08-15 |
DE69333112D1 (de) | 2003-08-28 |
FI950628A (fi) | 1995-02-13 |
JPH08500975A (ja) | 1996-02-06 |
JP3630424B2 (ja) | 2005-03-16 |
NO950534L (no) | 1995-04-05 |
DE69333112T2 (de) | 2004-06-09 |
US5714377A (en) | 1998-02-03 |
ES2203619T3 (es) | 2004-04-16 |
NZ255350A (en) | 1996-11-26 |
NO950534D0 (no) | 1995-02-13 |
EP0658203B1 (en) | 2003-07-23 |
EP0658203A1 (en) | 1995-06-21 |
DE4244915C2 (de) | 1998-12-03 |
ZA935915B (en) | 1995-04-13 |
DE4226971A1 (de) | 1994-02-17 |
CA2140894A1 (en) | 1994-03-03 |
NO320349B1 (no) | 2005-11-28 |
CA2140894C (en) | 2010-03-02 |
DE4226971C2 (de) | 1997-01-16 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
FI121075B (fi) | Geneettisesti muokattu sienisolu, menetelmä rekombinanttituotteiden tuottamiseksi sekä DNA-molekyyli ja DNA-fragmentti ja näiden käyttö | |
JAUNIAUX et al. | GAP1, the general amino acid permease gene of Saccharomyces cerevisiae: nucleotide sequence, protein similarity with the other bakers yeast amino acid permeases, and nitrogen catabolite repression | |
KR101298157B1 (ko) | 2-미크론 패밀리 플라스미드 및 이의 용도 | |
KR101262682B1 (ko) | 유전자 발현 기술 | |
US5593858A (en) | Highly stable recombinant yeasts for the production of recombinant proteins | |
AU725340B2 (en) | Improved methods for transforming Phaffia strains, transformed Phaffia strains so obtained and recombinant DNA in said methods | |
US5939287A (en) | Method for production of proteins in yeast | |
San Segundo et al. | SSG1, a gene encoding a sporulation-specific 1, 3-beta-glucanase in Saccharomyces cerevisiae | |
FI104637B (fi) | Uudet yhdistelmä-DNA-molekyylit sekä bakteeri- ja sieni-isännät yhdistelmäinterferonien ja pektiinilyaasien tuottamiseksi | |
EP0760854A1 (en) | Lag1: a gene for increasing the longevity of eukaryotes | |
FI110006B (fi) | Hiivamutantit, joilla on vajavaisuus N-glykosylaatiossa | |
Kubelik et al. | The Neurospora crassa cyt-20 gene encodes cytosolic and mitochondrial valyl-tRNA synthetases and may have a second function in addition to protein synthesis | |
Zou et al. | Asparagine-linked glycosylation in Schizosaccharomyces pombe: functional conservation of the first step in oligosaccharide-lipid assembly | |
Mautino et al. | eth-1, the Neurospora crassa locus encoding S-adenosylmethionine synthetase: molecular cloning, sequence analysis and in vivo overexpression | |
Le Dall et al. | The 3-phosphoglycerate kinase gene of the yeast Yarrowia lipolytica de-represses on gluconeogenic substrates | |
KR20020008174A (ko) | 재조합 단백질의 생산 방법 | |
JPH09266792A (ja) | 酵母のマンノース−1−リン酸転移を正に制御する遺伝子ならびにこの遺伝子の欠損変異株を利用する高マンノース型中性糖鎖の製造方法 | |
JP2767401B2 (ja) | 酵母のマンノース−1−リン酸転移酵素遺伝子およびこれを利用するマンノース−1−リン酸含有酸性糖鎖の製造方法 | |
JP2000125859A (ja) | 新規な選択マーカー遺伝子ならびにリゾムコール・プシルスの形質転換系 | |
JP2000189166A (ja) | リゾムコ―ル属糸状菌の糖鎖付加能欠損変異株を用いたタンパク質の製造法 | |
Mautino et al. | eth-1, the Neurospora wmsa Locus Encoding S-Adenosylmethionine Synthetase: Molecular Cloning, Sequence Analysis and in Vivo Overexpression | |
Mautino et al. | Eth-1, the Neurospora crassa Locus encoding... |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PC | Transfer of assignment of patent |
Owner name: NOVOZYMES BIOPHARMA UK LIMITED Free format text: NOVOZYMES BIOPHARMA UK LIMITED |
|
PC | Transfer of assignment of patent |
Owner name: NOVOZYMES BIOPHARMA DK A/S Free format text: NOVOZYMES BIOPHARMA DK A/S |
|
FG | Patent granted |
Ref document number: 121075 Country of ref document: FI |
|
MA | Patent expired |