FI112667B - Nytt svampproteas - Google Patents

Nytt svampproteas Download PDF

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Publication number
FI112667B
FI112667B FI931634A FI931634A FI112667B FI 112667 B FI112667 B FI 112667B FI 931634 A FI931634 A FI 931634A FI 931634 A FI931634 A FI 931634A FI 112667 B FI112667 B FI 112667B
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FI
Finland
Prior art keywords
dna
sequence
ala
gene
ser
Prior art date
Application number
FI931634A
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English (en)
Finnish (fi)
Other versions
FI931634A0 (fi
FI931634A (fi
Inventor
Frank Buxton
Jacob Visser
Albert Hinnen
Original Assignee
Novartis Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB939305097A external-priority patent/GB9305097D0/en
Application filed by Novartis Ag filed Critical Novartis Ag
Publication of FI931634A0 publication Critical patent/FI931634A0/fi
Publication of FI931634A publication Critical patent/FI931634A/fi
Application granted granted Critical
Publication of FI112667B publication Critical patent/FI112667B/sv

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/62—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Detergent Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Claims (10)

1. Isolerad DNA-molekyl, kännetecknad därav, att den innehäl-ler en DNA-sekvens, som kodar för ett Aspergillus niger se- 5 rinproteas, som är ett serinproteas med aminosyrasekvensen angiven i Sekvens 2 eller i Sekvens 7 av Sekvenslistan.
2. DNA-molekyl enligt patentkravet 1, kännetecknad därav, att den innehäller en DNA-sekvens, som är kodingsregionen för 10 pepC angiven i Sekvens 1 eller kodingsregionen för pepD angiven i Sekvens 6.
3. Hybridvektor, kännetecknad därav, att den innehäller en DNA-molekyl enligt patentkravet 1. 15
4. Hybridvektor enligt patentkravet 3, kännetecknad därav, att DNA-sekvensen, som kodar för ett Aspergillus niger serinproteas av subtilisintyp, är funktionellt kopplad till re-gleringselement, som är lämpliga för att uttrycka en sädan
20 DNA-sekvens i en lämplig värdcell.
5. Hybridvektor enligt patentkravet 4, kännetecknad därav, 'f att DNA-sekvensen, som kodar för ett Aspergillus niger serin- ♦ a i » 1 proteas av subtilisintyp, är funktionellt kopplad tili re- < » I • ·’ 25 gleringselement, som är lämpliga för att uttrycka en sädan '' ' DNA-sekvens i en Aspergillus-stam.
6. Aspergillus niger-stam, kännetecknad därav, att den är As-pergillus niger, som har ett fel i pepC-gensekvensen enligt V' 30 Sekvens 1, Aspergillus niger, som har ett fel i pepD-gen- sekvensen enligt Sekvens 6 eller Aspergillus niger, som har : ett fel i pepC- respektive i pepD-gensekvenserna enligt Se- " kvens 1 och Sekvens 6, varvid DNA-sekvensen inte mera förmär uttrycka ett funktionellt protein. 104 112 667
7. Förfarande för att framställa en önskad polypeptid, känne-tecknat därav, att en Aspergillus niger-stam enligt patent-kravet 6 transformeras med en uttryckningsvektor, som inne- 5 häller en uttryckningskassett, som är lämplig för att ut- trycka den önskade polypeptiden, den transformerade Aspergillus niger-stammen odlas i förhällanden, som är lampiiga för att uttrycka nämnda polypeptid, och den önskade polypeptiden isoleras. 10
8. Serinproteas av Aspergillus niger, kännetecknat därav, att det är PEPC angivet i Sekvens 2 eller PEPD angivet i Sekvens 7 .
9. En värd transformerad med en hybriduttryckningsvektor en ligt patentkravet 3, kännetecknad därav, att vektorn innehäl-ler en DNA-sekvens, som kodar för ett Aspergillus niger serinproteas av subtilisintyp, och som är funktionellt kopplad tili regleringselement, som är lämpliga för att uttrycka en 20 sadan DNA-sekvens i nämnda värd.
10. Transformerad värd enligt patentkravet 9, kännetecknad » därav, att den är en Aspergillus niger-stam.
FI931634A 1992-04-15 1993-04-08 Nytt svampproteas FI112667B (sv)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP92810281 1992-04-15
EP92810281 1992-04-15
GB9305097 1993-03-12
GB939305097A GB9305097D0 (en) 1993-03-12 1993-03-12 Noval fungal protease

Publications (3)

Publication Number Publication Date
FI931634A0 FI931634A0 (fi) 1993-04-08
FI931634A FI931634A (fi) 1993-10-16
FI112667B true FI112667B (sv) 2003-12-31

Family

ID=26132501

Family Applications (1)

Application Number Title Priority Date Filing Date
FI931634A FI112667B (sv) 1992-04-15 1993-04-08 Nytt svampproteas

Country Status (17)

Country Link
EP (1) EP0574347B1 (sv)
JP (2) JP3424840B2 (sv)
KR (1) KR100270644B1 (sv)
AT (1) ATE252156T1 (sv)
AU (1) AU663173B2 (sv)
CA (1) CA2093950C (sv)
CY (1) CY2507B1 (sv)
DE (1) DE69333249T2 (sv)
DK (1) DK0574347T3 (sv)
ES (1) ES2208637T3 (sv)
FI (1) FI112667B (sv)
HU (1) HU219809B (sv)
IL (2) IL105383A0 (sv)
MX (1) MX9302202A (sv)
NO (1) NO315379B1 (sv)
NZ (1) NZ247397A (sv)
PT (1) PT574347E (sv)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5821350A (en) * 1995-11-01 1998-10-13 Nexia Biotechnologies, Inc. Aspergillus niger beta-galactosidase gene
EP0866872A1 (en) * 1995-12-15 1998-09-30 Novo Nordisk A/S A FUNGUNS WHEREIN THE areA, pepC AND/OR pepE GENES HAVE BEEN INACTIVATED
WO1997035956A1 (en) * 1996-03-27 1997-10-02 Novo Nordisk A/S Alkaline protease deficient filamentous fungi
WO1997046689A1 (en) * 1996-06-05 1997-12-11 Gist-Brocades B.V. Fungal metallo protease genes
JPH1016992A (ja) * 1996-07-04 1998-01-20 Toagosei Co Ltd 接着剤用容器
US6806062B1 (en) 1998-10-05 2004-10-19 Novozymes A/S Fungal transcriptional activator useful in methods for producing polypeptides
AU2001242299A1 (en) 2000-03-14 2001-09-24 Novozymes A/S Fungal transcriptional activator useful in methods for producing polypeptides
CN101292024B (zh) * 2005-10-17 2012-04-18 诺维信公司 用于表达抗体的真菌突变体的用途
EP2220110A1 (en) 2007-12-06 2010-08-25 Novozymes A/S Fungal pepc inhibitor
BR122019011255B1 (pt) 2009-12-18 2020-12-01 Novozymes, Inc mutante de uma cepa de trichoderma reesei parental isolada, e, métodos para produzir um polipeptídeo e para obter um mutante de uma cepa de trichoderma reesei parental
MD4186C1 (ro) * 2012-02-20 2013-06-30 Институт Микробиологии И Биотехнологии Академии Наук Молдовы Tulpină de fungi Fusarium gibbosum - producătoare de proteaze acide şi neutre, xilanaze şi b-glucozidaze
JP2016523552A (ja) 2013-07-10 2016-08-12 ノバルティス アーゲー 多重プロテアーゼ欠損糸状菌細胞及びその使用方法
WO2015025055A1 (en) * 2013-08-23 2015-02-26 Novozymes A/S Regulated pepc expression
WO2016012468A1 (en) 2014-07-21 2016-01-28 Novartis Ag Production of glycoproteins with mammalian-like n-glycans in filamentous fungi

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1333777C (en) * 1988-07-01 1995-01-03 Randy M. Berka Aspartic proteinase deficient filamentous fungi

Also Published As

Publication number Publication date
HU9301087D0 (en) 1993-06-28
FI931634A0 (fi) 1993-04-08
FI931634A (fi) 1993-10-16
NZ247397A (en) 1995-08-28
CA2093950A1 (en) 1993-10-16
JP2003235591A (ja) 2003-08-26
NO315379B1 (no) 2003-08-25
DE69333249D1 (de) 2003-11-20
JP3424840B2 (ja) 2003-07-07
CY2507B1 (en) 2005-12-23
JPH0646863A (ja) 1994-02-22
DE69333249T2 (de) 2004-07-29
IL105383A0 (en) 1993-08-18
KR930021783A (ko) 1993-11-23
HUT67800A (en) 1995-05-29
KR100270644B1 (ko) 2000-11-01
PT574347E (pt) 2004-02-27
DK0574347T3 (da) 2004-01-26
EP0574347A3 (sv) 1994-04-13
CA2093950C (en) 2006-05-09
MX9302202A (es) 1994-04-29
EP0574347A2 (en) 1993-12-15
NO931368D0 (no) 1993-04-14
HU219809B (hu) 2001-08-28
IL170763A (en) 2007-10-31
NO931368L (no) 1993-10-18
AU663173B2 (en) 1995-09-28
JP3636455B2 (ja) 2005-04-06
ATE252156T1 (de) 2003-11-15
AU3695993A (en) 1993-10-21
EP0574347B1 (en) 2003-10-15
ES2208637T3 (es) 2004-06-16

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Owner name: NOVARTIS AG

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