FI111415B - Elektrokemiluminescensförfarande - Google Patents

Elektrokemiluminescensförfarande Download PDF

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Publication number
FI111415B
FI111415B FI955252A FI955252A FI111415B FI 111415 B FI111415 B FI 111415B FI 955252 A FI955252 A FI 955252A FI 955252 A FI955252 A FI 955252A FI 111415 B FI111415 B FI 111415B
Authority
FI
Finland
Prior art keywords
bmg
analyte
solution
hsap
nacl
Prior art date
Application number
FI955252A
Other languages
English (en)
Finnish (fi)
Other versions
FI955252A (sv
FI955252A0 (sv
Inventor
Guenter Mueller
Ulrich Neumann
Ursula Giesen
Nicholas Hoyle
Volker Klemt
Original Assignee
Roche Diagnostics Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE4401577A external-priority patent/DE4401577A1/de
Application filed by Roche Diagnostics Gmbh filed Critical Roche Diagnostics Gmbh
Publication of FI955252A publication Critical patent/FI955252A/sv
Publication of FI955252A0 publication Critical patent/FI955252A0/sv
Application granted granted Critical
Publication of FI111415B publication Critical patent/FI111415B/sv

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/66—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light electrically excited, e.g. electroluminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence

Claims (10)

1. Förfarande för mätning av elektrokemiluminescensfenomen i en lösning eller i en fast fas som avgränsas mot en lösning 20 genom att föra en komponent som är kapabel tili elektro-kemiluminescens i beröring med ett oxiderbart amin, och genom att utsätta den för en elektronisk spänning i syfte att inducera elektrokemiluminescens, och genom att detektera den elektromagnetiska strälningen, kännetecknat 25 av att lösningen innehäller en detergent, vilken är fett-alkoholetoxylater, Plantaren och/eller oktylglukosid.
2. Förfarande enligt patentkrav 1, kännetecknat 30 av att som fettalkoholetoxylat används Polidocanol, C14-E09, Genapol eller C8-E09.
3. Förfarande enligt patentkrav 1 eller 2, kännetecknat av att lösningen innehäller en eller flera alkali- 35 eller jordalkalimetallhalogenider.
4. Förfarande enligt patentkraven 1-3, känneteck- 111415 n a t av att lösningens pH-värde är mellan 6,5 och 9,0.
5. Förfarande enligt patentkraven 1-4, känneteck-n a t av att elektrokemiluminescensen avstäms genom att 5 använda maximalt 2,2 V rektangulärspänning eller maximalt 3,0 V rampspännig.
6. Förfarande enligt nägot av patentkraven 1-5 för detektering av en analyt, kännetecknat av att 10 komponenten som är kapabel tili elektrokemiluminescens är ett tecken pä en analyt, en analytanalog eller ett analytspecifikt ämne, och strälningen som detekteras är mättet pä analytens närvaro.
7. Reagenslösning för att mätä elektrokemiska fenomen, innefattande elektrokemiskt oxiderbart amin, som är ett kraftigt reduktionsmedel i oxiderat tillständ, k ä n n e-tecknad av att den innefattar en detergent, som är fettalkoholetoxylat, Plantaren och/eller oktylglukosid. 20
8. Reagenslösning enligt patentkrav 6, känneteck-n a d av att den innefattar detergent i en koncentration av 0,001 - 1 %.
9. Reagenslösning enligt patentkrav 6 eller 7, k ä n n e-tecknad av att den dessutom innefattar alkalklorid i en koncentration av 0,05 - 0,5 mol/1.
10. Reagenslösning enligt patentkrav 6-8, känne-30tecknad av att dess pH-värde är mellan 6,5 och 9,0.
FI955252A 1993-05-03 1995-11-02 Elektrokemiluminescensförfarande FI111415B (sv)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
DE4314547 1993-05-03
DE4314547 1993-05-03
DE4332697 1993-09-25
DE4332697 1993-09-25
DE4401577 1994-01-20
DE4401577A DE4401577A1 (de) 1993-05-03 1994-01-20 Elektrochemilumineszenzverfahren
EP9401328 1994-04-27
PCT/EP1994/001328 WO1994025853A1 (de) 1993-05-03 1994-04-27 Elektrochemilumineszenzverfahren

Publications (3)

Publication Number Publication Date
FI955252A FI955252A (sv) 1995-11-02
FI955252A0 FI955252A0 (sv) 1995-11-02
FI111415B true FI111415B (sv) 2003-07-15

Family

ID=27205044

Family Applications (1)

Application Number Title Priority Date Filing Date
FI955252A FI111415B (sv) 1993-05-03 1995-11-02 Elektrokemiluminescensförfarande

Country Status (14)

Country Link
US (2) US5677192A (sv)
EP (2) EP0697106B1 (sv)
JP (2) JP2735688B2 (sv)
CN (1) CN1089899C (sv)
AT (2) ATE161955T1 (sv)
AU (2) AU674079B2 (sv)
CA (2) CA2161704C (sv)
ES (1) ES2102858T3 (sv)
FI (1) FI111415B (sv)
IL (1) IL109481A0 (sv)
NO (2) NO954387L (sv)
NZ (1) NZ266149A (sv)
RU (1) RU2116647C1 (sv)
WO (2) WO1994025854A1 (sv)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1130563C (zh) * 1996-09-19 2003-12-10 第一化学药品株式会社 免疫组织化学染色用组合物及其用途
US6146838A (en) * 1997-03-18 2000-11-14 Igen International, Inc. Detecting water-borne parasites using electrochemiluminescence
JP2010237020A (ja) * 2009-03-31 2010-10-21 Japan Aerospace Exploration Agency 生体分子検出装置及び生体分子検出方法
AU2011322704B2 (en) * 2010-10-25 2014-12-11 F. Hoffmann-La Roche Ag Use of signal enhancing compounds in electrochemiluminescence detection
KR101636250B1 (ko) * 2014-12-12 2016-07-07 주식회사 도건이엔텍 헬리컬 파일
CN104698164B (zh) * 2015-02-13 2017-03-15 中山市创艺生化工程有限公司 一种电化学发光免疫分析仪用缓冲液及其制备方法
CN105929010A (zh) * 2016-04-14 2016-09-07 福建师范大学 一种基于曙红y的电致化学发光传感器
KR101980079B1 (ko) 2018-12-06 2019-08-28 주식회사 강탄산업 인발저항용 앵커를 갖는 방음벽기초의 시공방법
CN113295678B (zh) * 2021-05-14 2022-11-08 济南迪曼生物科技有限公司 一种电化学发光清洗液

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5227399A (en) * 1975-08-27 1977-03-01 Seiko Instr & Electronics Ltd Driving system of electrochemical luminous element
JPS5247324A (en) * 1975-10-14 1977-04-15 Seiko Instr & Electronics Ltd Driving system of display equipment
US4462931A (en) * 1982-06-16 1984-07-31 American Cyanamid Company Enhanced aqueous chemiluminescent systems
US4927769A (en) * 1987-07-08 1990-05-22 Ciba Corning Diagnostics Corp. Method for enhancement of chemiluminescence
IL90105A (en) * 1988-04-29 1993-05-13 Igen Inc Method and apparatus for conducting electro- chemiluminescence measurements
ATE161096T1 (de) * 1988-11-03 1997-12-15 Igen Inc Elektrochemilumineszente reaktion unter verwendung eines von einem amin abgeleiteten reduktionsmittels
US5061445A (en) * 1988-11-03 1991-10-29 Igen, Inc. Apparatus for conducting measurements of electrochemiluminescent phenomena
CA2002083C (en) * 1988-11-03 2001-01-09 Haresh P. Shah Enhanced electrochemiluminescence
US5324457A (en) * 1989-10-02 1994-06-28 Board Of Regents, The University Of Tx System Devices and methods for generating electrogenerated chemiluminescence
JPH07111400B2 (ja) * 1991-02-28 1995-11-29 株式会社島津製作所 発光測定法
US5466416A (en) * 1993-05-14 1995-11-14 Ghaed; Ali Apparatus and methods for carrying out electrochemiluminescence test measurements

Also Published As

Publication number Publication date
CA2161666C (en) 1999-08-10
CN1089899C (zh) 2002-08-28
JPH08508570A (ja) 1996-09-10
EP0697107B1 (de) 1998-01-07
AU6722394A (en) 1994-11-21
CA2161704C (en) 2002-01-22
JP3135921B2 (ja) 2001-02-19
NO954387D0 (no) 1995-11-02
AU6794294A (en) 1994-11-21
WO1994025853A1 (de) 1994-11-10
AU674079B2 (en) 1996-12-05
RU2116647C1 (ru) 1998-07-27
IL109481A0 (en) 1994-07-31
EP0697106B1 (de) 1997-04-02
JP2735688B2 (ja) 1998-04-02
NO954386D0 (no) 1995-11-02
AU678018B2 (en) 1997-05-15
CA2161704A1 (en) 1994-11-10
US5723342A (en) 1998-03-03
NO954386L (no) 1996-01-02
CA2161666A1 (en) 1994-11-10
WO1994025854A1 (de) 1994-11-10
CN1125480A (zh) 1996-06-26
NO954387L (no) 1996-01-02
US5677192A (en) 1997-10-14
EP0697106A1 (de) 1996-02-21
NZ266149A (en) 1996-11-26
ATE161955T1 (de) 1998-01-15
FI955252A (sv) 1995-11-02
ATE151171T1 (de) 1997-04-15
FI955252A0 (sv) 1995-11-02
NO318946B1 (no) 2005-05-30
ES2102858T3 (es) 1997-08-01
JPH08508102A (ja) 1996-08-27
EP0697107A1 (de) 1996-02-21

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