ES8701840A1 - Un procedimiento para preparar un activador de plasminogeno bicatenario purificado - Google Patents

Un procedimiento para preparar un activador de plasminogeno bicatenario purificado

Info

Publication number
ES8701840A1
ES8701840A1 ES548974A ES548974A ES8701840A1 ES 8701840 A1 ES8701840 A1 ES 8701840A1 ES 548974 A ES548974 A ES 548974A ES 548974 A ES548974 A ES 548974A ES 8701840 A1 ES8701840 A1 ES 8701840A1
Authority
ES
Spain
Prior art keywords
double
chain
plasminogen activator
aforesaid
affinity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES548974A
Other languages
English (en)
Other versions
ES548974A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Asahi Kasei Corp
Kowa Co Ltd
Asahi Chemical Industry Co Ltd
Original Assignee
Kowa Co Ltd
Asahi Chemical Industry Co Ltd
Asahi Kasei Kogyo KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP59013455A external-priority patent/JPS60158116A/ja
Priority claimed from JP59013456A external-priority patent/JPS60158117A/ja
Application filed by Kowa Co Ltd, Asahi Chemical Industry Co Ltd, Asahi Kasei Kogyo KK filed Critical Kowa Co Ltd
Publication of ES548974A0 publication Critical patent/ES548974A0/es
Publication of ES8701840A1 publication Critical patent/ES8701840A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

PROCEDIMIENTO PARA LA OBTENCION DE UN ACTIVADOR DE PLASMINOGENO BICATENARIO DE PESO MOLECULAR 63.000G10.000, PUNTO ISOELECTRICO ENTRE 7,0 Y 8,5; AFINIDAD PARA FIBRINA Y CONCANAVALINA A, PH ENTRE 7 Y 9,5, QUE SE DESCOMPONE POR REDUCCION, Y TIENE ACTIVIDAD DE HIDROLISIS FRENTE A DIFERENTES SUSTRATOS. CONSISTE EN INCUBAR UNA FRACCION QUE CONTIENE EL ACTIVADOR DE PLASMINOGENO RECOGIDO DE UN LIQUIDO DE CULTIVO DE TEJIDOS DE CELULAS DERIVADAS DE TEJIDOS HUMANOS NORMALES, EN CUALQUIER ETAPA DE SU SEPARACION Y PURIFICACION A UNA TEMPERATURA ENTRE 10 Y 80º DURANTE 5 A 10 DIAS O BIEN EN PRESENCIA DE UNA ENZIMA (PLASMINA) DE 10 A 45º DURANTE UN TIEMPO ENTRE 5 MINUTOS Y 3 HORAS. DE APLICACION COMO AGENTE TROMBOLITICO.
ES548974A 1984-01-30 1985-11-16 Un procedimiento para preparar un activador de plasminogeno bicatenario purificado Expired ES8701840A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP59013455A JPS60158116A (ja) 1984-01-30 1984-01-30 新規なプラスミノ−ゲン・アクチベ−タ−およびその製法ならびにこれを含有する薬剤
JP59013456A JPS60158117A (ja) 1984-01-30 1984-01-30 新規なプラスミノ−ゲン・アクチベ−タ−およびその製法ならびにこれを含有する薬剤

Publications (2)

Publication Number Publication Date
ES548974A0 ES548974A0 (es) 1986-12-01
ES8701840A1 true ES8701840A1 (es) 1986-12-01

Family

ID=26349257

Family Applications (2)

Application Number Title Priority Date Filing Date
ES539925A Expired ES8605583A1 (es) 1984-01-30 1985-01-29 Un procedimiento de producir un activador de plasminogeno monocatenario
ES548974A Expired ES8701840A1 (es) 1984-01-30 1985-11-16 Un procedimiento para preparar un activador de plasminogeno bicatenario purificado

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES539925A Expired ES8605583A1 (es) 1984-01-30 1985-01-29 Un procedimiento de producir un activador de plasminogeno monocatenario

Country Status (3)

Country Link
EP (1) EP0151996B1 (es)
DE (1) DE3582338D1 (es)
ES (2) ES8605583A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61176532A (ja) * 1985-01-30 1986-08-08 Green Cross Corp:The プラスミノ−ゲンアクチベ−タ−前駆体の安定化方法
KR940003056B1 (ko) * 1985-04-16 1994-04-13 가부시끼가이샤 미도리 쥬우지 우로키나제 전구체를 안정화시키는 방법
JPS624233A (ja) * 1985-07-01 1987-01-10 Toyobo Co Ltd 組織性プラスミノ−ゲン活性化因子の製造法
CA2011548A1 (en) * 1989-03-07 1990-09-07 Hubertus Stockinger Process for the production of two-chain t-pa
US5576605A (en) * 1992-11-20 1996-11-19 Seiko Epson Corporation Micro robot and holding case therefor

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4381346A (en) * 1979-11-13 1983-04-26 Huasin Syed S Isolation of plasminogen activators useful as therapeutic and diagnostic agents
NL8003402A (nl) * 1980-06-11 1982-01-04 Leuven Res & Dev Vzw Nieuwe plasminogeen-activator en farmaceutisch preparaat met trombolytische werking.
ZA831399B (en) * 1982-03-05 1984-02-29 Health Lab Service Board New fibrinolytic enzymes and methods for their production and pharmaceutical compositions containing them
JPS5913732A (ja) * 1982-07-16 1984-01-24 Mitsui Toatsu Chem Inc 血栓溶解剤
JPS5951220A (ja) * 1982-08-02 1984-03-24 Asahi Chem Ind Co Ltd 新規なプラスミノ−ゲン・アクチベ−タ−およびその製法ならびにこれを含有する薬剤
WO1984001714A1 (en) * 1982-10-29 1984-05-10 Mitsui Toatsu Chemicals Novel plasminogen activator, process for its preparation, and thrombolytic drug containing the same

Also Published As

Publication number Publication date
ES548974A0 (es) 1986-12-01
DE3582338D1 (de) 1991-05-08
EP0151996A2 (en) 1985-08-21
ES539925A0 (es) 1986-03-16
EP0151996B1 (en) 1991-04-03
ES8605583A1 (es) 1986-03-16
EP0151996A3 (en) 1986-10-01

Similar Documents

Publication Publication Date Title
ES8505405A1 (es) Un procedimiento para producir un activador de plasminogeno purificado.
AU605124B2 (en) Novel peptide plasminogen activators
ES8101385A1 (es) Un procedimiento para la preparacion de un derivado de una enzima fibrinolitica
HUT42955A (en) Process for production of plasminogene activator compositions of stabilized human-tissue
DE68918015D1 (de) Manganarmes proteinreiches Reismehl.
AU2165088A (en) Bacterial treatment to preserve hay quality by addition of microorganisms of the genus bacillus
ES8800982A1 (es) Un metodo para producir la enzima superoxido-dismutasa modificada con derivados de polietilenglicol y triazina.
AU4898385A (en) Enzymes
ES8605583A1 (es) Un procedimiento de producir un activador de plasminogeno monocatenario
DZ658A1 (fr) Antibiotiques produits par kibdelosporagium aridumet leur préparation.
HUT59318A (en) Process for stabilizing k2p pro t-pa
MY102800A (en) Peptide antibiotics
AU7037591A (en) Stabilization of t-pa pro
IE34196B1 (en) Improvements in and relating to pepsin inhibitors
GB1510323A (en) Biologically active substance bestatin derivatives thereof and production process thereof
KR890701733A (ko) 신규 인체 조직형 플리스미노겐 활성제 변이체
RO112643B1 (ro) Procedeu de preparare a aminoacidului-d sau a derivaţilor aminoacidului-d
ES8405760A1 (es) Metodo para la preparacion de insulina humana semisintetica.
AU2601088A (en) Hypoglycaemic peptides
EP0481003A2 (en) Process for producing antibacterial intermediates via enzyme hydrolysis of racemic substrates.
SU576336A1 (ru) Способ получени протеолитических ферментов
EP0217376A3 (en) Process for preparing optically active alpha-haloalkyl-aprocess for preparing optically active alpha-haloalkyl-arylketones rylketones
ES8605584A1 (es) Un procedimiento para preparar foroximitina
JPS5261286A (en) Preparation of enzymes
SU1520844A1 (ru) Способ получения l-лизин- альфа -оксидазы