ES2954516T3 - Sistema de coexpresión inducible - Google Patents

Sistema de coexpresión inducible Download PDF

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Publication number
ES2954516T3
ES2954516T3 ES19187102T ES19187102T ES2954516T3 ES 2954516 T3 ES2954516 T3 ES 2954516T3 ES 19187102 T ES19187102 T ES 19187102T ES 19187102 T ES19187102 T ES 19187102T ES 2954516 T3 ES2954516 T3 ES 2954516T3
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expression
promoter
gene
cells
inducible
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Sean Mcclain
Mark Valasek
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Absci Corp
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Absci Corp
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241Tumor Necrosis Factors
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424Serine endopeptidases (3.4.21)
    • C12N9/6429Thrombin (3.4.21.5)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/10Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14Specific host cells or culture conditions, e.g. components, pH or temperature
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
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    • C07K2317/00Immunoglobulins specific features
    • C07K2317/20Immunoglobulins specific features characterized by taxonomic origin
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/20Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/20Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/90Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/92Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2830/00Vector systems having a special element relevant for transcription
    • C12N2830/001Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0051Oxidoreductases (1.) acting on a sulfur group of donors (1.8)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90Isomerases (5.)
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93Ligases (6)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y108/00Oxidoreductases acting on sulfur groups as donors (1.8)
    • C12Y108/01Oxidoreductases acting on sulfur groups as donors (1.8) with NAD+ or NADP+ as acceptor (1.8.1)
    • C12Y108/01007Glutathione-disulfide reductase (1.8.1.7), i.e. glutathione reductase (NADPH)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21Serine endopeptidases (3.4.21)
    • C12Y304/21005Thrombin (3.4.21.5)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y503/00Intramolecular oxidoreductases (5.3)
    • C12Y503/04Intramolecular oxidoreductases (5.3) transposing S-S bonds (5.3.4)
    • C12Y503/04001Protein disulfide-isomerase (5.3.4.1), i.e. disufide bond-forming enzyme
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y603/00Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/02Acid—amino-acid ligases (peptide synthases)(6.3.2)
    • C12Y603/02003Glutathione synthase (6.3.2.3)

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Pulse, Heart Rate, Blood Pressure Or Blood Flow (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Slot Machines And Peripheral Devices (AREA)
  • Circuit Arrangement For Electric Light Sources In General (AREA)
  • Two-Way Televisions, Distribution Of Moving Picture Or The Like (AREA)
ES19187102T 2012-08-05 2013-08-05 Sistema de coexpresión inducible Active ES2954516T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261679751P 2012-08-05 2012-08-05
US201261747246P 2012-12-29 2012-12-29

Publications (1)

Publication Number Publication Date
ES2954516T3 true ES2954516T3 (es) 2023-11-22

Family

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Family Applications (3)

Application Number Title Priority Date Filing Date
ES13756743.4T Active ES2621320T3 (es) 2012-08-05 2013-08-05 Sistema de coexpresión inducible
ES16201998T Active ES2750005T3 (es) 2012-08-05 2013-08-05 Sistema de coexpresión inducible
ES19187102T Active ES2954516T3 (es) 2012-08-05 2013-08-05 Sistema de coexpresión inducible

Family Applications Before (2)

Application Number Title Priority Date Filing Date
ES13756743.4T Active ES2621320T3 (es) 2012-08-05 2013-08-05 Sistema de coexpresión inducible
ES16201998T Active ES2750005T3 (es) 2012-08-05 2013-08-05 Sistema de coexpresión inducible

Country Status (11)

Country Link
US (8) US9617335B2 (enExample)
EP (4) EP2880168B1 (enExample)
JP (3) JP6457387B2 (enExample)
CN (2) CN111748510B (enExample)
AU (3) AU2013299910B2 (enExample)
CA (1) CA2880285C (enExample)
DK (2) DK2880168T3 (enExample)
ES (3) ES2621320T3 (enExample)
IL (3) IL236975B (enExample)
PL (1) PL3591057T3 (enExample)
WO (1) WO2014025663A1 (enExample)

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JP7032292B2 (ja) 2022-03-08
US10604761B2 (en) 2020-03-31
US20200172915A1 (en) 2020-06-04
US20200063116A1 (en) 2020-02-27
EP4273257A2 (en) 2023-11-08
US10465197B2 (en) 2019-11-05
DK3187586T3 (da) 2019-10-14
DK2880168T3 (en) 2017-04-18
HK1210804A1 (en) 2016-05-06
EP3187586B1 (en) 2019-09-25
IL276141B (en) 2022-03-01
EP3591057A1 (en) 2020-01-08
JP2022068332A (ja) 2022-05-09
IL276141A (en) 2020-09-30
EP4273257A3 (en) 2024-02-28
EP2880168A1 (en) 2015-06-10
CA2880285C (en) 2022-11-01
ES2750005T3 (es) 2020-03-24
AU2019202844A1 (en) 2019-05-16
US9617335B2 (en) 2017-04-11
US11214807B2 (en) 2022-01-04
IL264384B (en) 2020-08-31
AU2022204192A1 (en) 2022-07-07
AU2013299910B2 (en) 2019-01-24
US20200032276A1 (en) 2020-01-30
US20160152701A1 (en) 2016-06-02
CN111748510B (zh) 2023-08-15
WO2014025663A1 (en) 2014-02-13
US11447781B2 (en) 2022-09-20
US20230340501A1 (en) 2023-10-26
EP3187586A1 (en) 2017-07-05
IL236975B (en) 2019-06-30
PL3591057T3 (pl) 2023-10-02
US20150225731A1 (en) 2015-08-13
CN104603274B (zh) 2020-08-18
CA2880285A1 (en) 2014-02-13
IL264384A (en) 2019-02-28
ES2621320T3 (es) 2017-07-03
US20170159061A1 (en) 2017-06-08
CN111748510A (zh) 2020-10-09
EP2880168B1 (en) 2017-01-11
US20220090093A1 (en) 2022-03-24
JP2019068830A (ja) 2019-05-09
AU2013299910A1 (en) 2015-03-12
IL236975A0 (en) 2015-03-31
CN104603274A (zh) 2015-05-06
JP2015524281A (ja) 2015-08-24
EP3591057C0 (en) 2023-06-07
EP3591057B9 (en) 2023-07-19
AU2019202844B2 (en) 2022-03-17
EP3591057B1 (en) 2023-06-07
JP6457387B2 (ja) 2019-01-23

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