ES2952405T3 - Parejas de fusión para la producción de péptidos - Google Patents
Parejas de fusión para la producción de péptidos Download PDFInfo
- Publication number
- ES2952405T3 ES2952405T3 ES15866061T ES15866061T ES2952405T3 ES 2952405 T3 ES2952405 T3 ES 2952405T3 ES 15866061 T ES15866061 T ES 15866061T ES 15866061 T ES15866061 T ES 15866061T ES 2952405 T3 ES2952405 T3 ES 2952405T3
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- fusion protein
- polypeptide
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- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 263
- 230000004927 fusion Effects 0.000 title claims abstract description 172
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 20
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- 229920001184 polypeptide Polymers 0.000 claims abstract description 245
- 108020001507 fusion proteins Proteins 0.000 claims abstract description 182
- 102000037865 fusion proteins Human genes 0.000 claims abstract description 179
- 108090000623 proteins and genes Proteins 0.000 claims description 316
- 102000004169 proteins and genes Human genes 0.000 claims description 273
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 claims description 219
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 claims description 219
- 230000014509 gene expression Effects 0.000 claims description 218
- 210000004027 cell Anatomy 0.000 claims description 187
- 238000003776 cleavage reaction Methods 0.000 claims description 121
- 241000589540 Pseudomonas fluorescens Species 0.000 claims description 119
- 230000007017 scission Effects 0.000 claims description 119
- 238000000034 method Methods 0.000 claims description 117
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- 108010013369 Enteropeptidase Proteins 0.000 claims description 87
- 108010076181 Proinsulin Proteins 0.000 claims description 71
- 108091005804 Peptidases Proteins 0.000 claims description 70
- 239000004365 Protease Substances 0.000 claims description 68
- 239000002773 nucleotide Substances 0.000 claims description 59
- 125000003729 nucleotide group Chemical group 0.000 claims description 59
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Classifications
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- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/635—Parathyroid hormone, i.e. parathormone; Parathyroid hormone-related peptides
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/53—Colony-stimulating factor [CSF]
- C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21009—Enteropeptidase (3.4.21.9), i.e. enterokinase
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
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- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Applications Claiming Priority (2)
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| US201462086119P | 2014-12-01 | 2014-12-01 | |
| PCT/US2015/063027 WO2016089782A1 (en) | 2014-12-01 | 2015-11-30 | Fusion partners for peptide production |
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| ES2952405T3 true ES2952405T3 (es) | 2023-10-31 |
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| ES15866061T Active ES2952405T3 (es) | 2014-12-01 | 2015-11-30 | Parejas de fusión para la producción de péptidos |
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| EP (1) | EP3227455B1 (https=) |
| JP (2) | JP6817939B2 (https=) |
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| US9798758B2 (en) | 2015-05-15 | 2017-10-24 | Foogal Incorporated | Food freshness notification from personal food inventory |
| JP7784804B2 (ja) | 2017-10-27 | 2025-12-12 | フェネックス インク． | 組み換えｅｒｗｉｎｉａアスパラギナーゼの製造のための方法 |
| WO2019083794A1 (en) | 2017-10-27 | 2019-05-02 | Pfenex Inc. | PROCESS FOR PRODUCING EAR ASPARAGINASE. RECOMBINED COLI |
| US11267863B2 (en) | 2018-01-19 | 2022-03-08 | Pepgene Inc. | N-terminal fusion partner for producing recombinant polypeptide, and method for producing recombinant polypeptide using same |
| CN108191981B (zh) * | 2018-02-06 | 2020-11-03 | 美药星（南京）制药有限公司 | 一种利拉鲁肽中间体多肽的制备方法 |
| KR102009709B1 (ko) * | 2018-02-14 | 2019-08-12 | 주식회사 펩진 | 융합 폴리펩타이드를 이용하여 인간 부갑상선 호르몬 1-84를 생산하는 방법 |
| KR102017542B1 (ko) * | 2018-02-14 | 2019-09-03 | 주식회사 펩진 | 융합 폴리펩타이드를 이용하여 글루카곤 유사 펩타이드-2 또는 이의 유사체를 생산하는 방법 |
| KR102017540B1 (ko) * | 2018-02-14 | 2019-09-03 | 주식회사 펩진 | 융합 폴리펩타이드를 이용하여 글루카곤 유사 펩타이드-1 또는 이의 유사체를 생산하는 방법 |
| CN108977423A (zh) * | 2018-08-17 | 2018-12-11 | 集美大学 | 一种从猪肺中分离提纯血管紧张素转化酶的方法 |
| KR102011291B1 (ko) * | 2018-12-05 | 2019-10-21 | 주식회사 펩진 | 신규한 융합 폴리펩타이드 및 이를 이용하여 인간 부갑상선 호르몬 1-34를 생산하는 방법 |
| US11364223B2 (en) | 2018-12-13 | 2022-06-21 | Huyabio International, Llc | Sulcardine administration for treatment of acute atrial fibrillation |
| CN110305223B (zh) * | 2019-06-26 | 2022-05-13 | 重庆派金生物科技有限公司 | 重组串联融合蛋白制备目标多肽的方法 |
| KR20210010368A (ko) * | 2019-07-19 | 2021-01-27 | 주식회사 펩진 | 글루카곤 유사 펩타이드-2 또는 이의 유사체 생산용 융합태그 |
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| WO2021091304A1 (ko) * | 2019-11-08 | 2021-05-14 | 한국해양과학기술원 | 신규한 인간 상피세포성장인자-tf 융합 단백질 및 이의 용도 |
| CN113773400B (zh) * | 2020-06-09 | 2023-08-18 | 宁波鲲鹏生物科技有限公司 | 一种门冬胰岛素衍生物及其应用 |
| WO2022016118A2 (en) * | 2020-07-17 | 2022-01-20 | Cb Therapeutics, Inc. | Prenyltransferase enzymes |
| CN113151296B (zh) * | 2021-03-22 | 2022-09-13 | 云南中烟工业有限责任公司 | 一种烟草热激蛋白相关的基因及其应用 |
| CN112941058B (zh) * | 2021-04-02 | 2023-12-05 | 重庆科润生物医药研发有限公司 | 一种重组溶组织梭菌ii型胶原酶及其制备方法和应用 |
| CN113025599B (zh) * | 2021-04-02 | 2023-09-12 | 重庆科润生物医药研发有限公司 | 一种重组溶组织梭菌i型胶原酶及其制备方法和应用 |
| KR20240021234A (ko) * | 2021-06-10 | 2024-02-16 | 펠리칸 테크놀로지 홀딩스, 인크. | 재조합 단백질 발현을 위한 박테리아 숙주 |
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| WO2023049631A2 (en) * | 2021-09-09 | 2023-03-30 | Northwestern University | Cell-free methods and compositions comprising tagless therapeutic hormones |
| CN114350587B (zh) * | 2022-01-24 | 2023-10-31 | 修实生物医药(南通)有限公司 | 一种基因重组串联表达利那洛肽的工程菌 |
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| KR20170085129A (ko) | 2017-07-21 |
| JP2017536835A (ja) | 2017-12-14 |
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| US20190135889A1 (en) | 2019-05-09 |
| SG11201704362VA (en) | 2017-06-29 |
| US20160159877A1 (en) | 2016-06-09 |
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