ES2751371T3 - Procedimiento de detección de polimorfismos de nucleótido único - Google Patents

Procedimiento de detección de polimorfismos de nucleótido único Download PDF

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Publication number
ES2751371T3
ES2751371T3 ES13798093T ES13798093T ES2751371T3 ES 2751371 T3 ES2751371 T3 ES 2751371T3 ES 13798093 T ES13798093 T ES 13798093T ES 13798093 T ES13798093 T ES 13798093T ES 2751371 T3 ES2751371 T3 ES 2751371T3
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primer
amplification
nucleic acid
oligonucleotide
amplification reaction
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Spanish (es)
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Shihai Huang
Hong Su
Brian Erickson
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Abbott Molecular Inc
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Abbott Molecular Inc
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6858Allele-specific amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/107Modifications characterised by incorporating a peptide nucleic acid
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/161A competitive reaction step
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2545/00Reactions characterised by their quantitative nature
    • C12Q2545/10Reactions characterised by their quantitative nature the purpose being quantitative analysis
    • C12Q2545/101Reactions characterised by their quantitative nature the purpose being quantitative analysis with an internal standard/control
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/166Oligonucleotides used as internal standards, controls or normalisation probes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
ES13798093T 2012-05-29 2013-05-28 Procedimiento de detección de polimorfismos de nucleótido único Active ES2751371T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261652827P 2012-05-29 2012-05-29
US13/840,142 US10077474B2 (en) 2012-05-29 2013-03-15 Method of designing primers, method of detecting single nucleotide polymorphisms (SNPs), method of distinguishing SNPs, and related primers, detectable oligonucleotides, and kits
PCT/US2013/042817 WO2013181125A2 (en) 2012-05-29 2013-05-28 Method of designing primers, method of detecting single nucleotide polymorphisms (snps), method of distinguishing snps, and related primers, detectable oligonucleotides, and kits

Publications (1)

Publication Number Publication Date
ES2751371T3 true ES2751371T3 (es) 2020-03-31

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ES13798093T Active ES2751371T3 (es) 2012-05-29 2013-05-28 Procedimiento de detección de polimorfismos de nucleótido único

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US (2) US10077474B2 (enExample)
EP (2) EP2855708B1 (enExample)
JP (2) JP6448534B2 (enExample)
AU (2) AU2013267660B2 (enExample)
CA (1) CA2875281A1 (enExample)
ES (1) ES2751371T3 (enExample)
WO (1) WO2013181125A2 (enExample)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11111543B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US9424392B2 (en) 2005-11-26 2016-08-23 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US12221653B2 (en) 2010-05-18 2025-02-11 Natera, Inc. Methods for simultaneous amplification of target loci
US12152275B2 (en) 2010-05-18 2024-11-26 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
JP6153874B2 (ja) 2011-02-09 2017-06-28 ナテラ, インコーポレイテッド 非侵襲的出生前倍数性呼び出しのための方法
AU2013249012B2 (en) 2012-04-19 2019-03-28 The Medical College Of Wisconsin, Inc. Highly sensitive surveillance using detection of cell free DNA
US20140100126A1 (en) 2012-08-17 2014-04-10 Natera, Inc. Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data
CN104928355A (zh) * 2014-03-19 2015-09-23 李跃 检测braf基因突变的方法及其试剂盒
US12492429B2 (en) 2014-04-21 2025-12-09 Natera, Inc. Detecting mutations and ploidy in chromosomal segments
CN113774132A (zh) 2014-04-21 2021-12-10 纳特拉公司 检测染色体片段中的突变和倍性
US20180173846A1 (en) 2014-06-05 2018-06-21 Natera, Inc. Systems and Methods for Detection of Aneuploidy
DK3294906T3 (en) 2015-05-11 2024-08-05 Natera Inc Methods for determining ploidy
EP3307911B1 (en) * 2015-06-11 2020-12-09 Bio-Rad Laboratories, Inc. Amplification assay with a probe competitor
US11053542B2 (en) 2015-12-31 2021-07-06 Quest Diagnostics Investments Llc Compositions and methods for screening mutations in thyroid cancer
DK3443119T3 (da) 2016-04-15 2022-05-23 Natera Inc Fremgangsmåde til bestemmelse af lunge cancer
EA201892491A1 (ru) * 2016-04-29 2019-06-28 Дзе Медикал Колледж Оф Висконсин, Инк. Пцр в реальном времени с мультиплексной оптимизированной амплификацией неправильно спаренных оснований (moma) для анализа злокачественных новообразований
CN105861689A (zh) * 2016-05-06 2016-08-17 北京晋祺生物科技有限公司 一种cyp3a5*3的检测引物组合物、试剂盒及方法
GB201618485D0 (en) 2016-11-02 2016-12-14 Ucl Business Plc Method of detecting tumour recurrence
EP3642354B1 (en) 2017-06-20 2022-08-03 Bio-Rad Laboratories, Inc. Digital amplification assays for genetic instability
JP2020524519A (ja) 2017-06-20 2020-08-20 ザ メディカル カレッジ オブ ウィスコンシン,インコーポレイテッドThe Medical College of Wisconsin, Inc. 全セルフリーdnaによる移植合併症リスクの評価
WO2019099420A1 (en) * 2017-11-15 2019-05-23 Yan Wang A method for detecting multiple dna mutations and copy number variations
WO2019118926A1 (en) 2017-12-14 2019-06-20 Tai Diagnostics, Inc. Assessing graft suitability for transplantation
DK3781714T3 (da) 2018-04-14 2026-03-30 Natera Inc Fremgangsmåder til cancerpåvisning og -overvågning ved personlig påvisning af cirkulerende tumor-dna
US12234509B2 (en) 2018-07-03 2025-02-25 Natera, Inc. Methods for detection of donor-derived cell-free DNA
CA3125435A1 (en) * 2018-12-31 2020-07-09 Abbott Molecular Inc. Improved amplification of nucleic acids
WO2020145734A1 (ko) * 2019-01-11 2020-07-16 주식회사 진캐스트 Braf 돌연변이 검출을 위한 dna 중합효소 및 이를 포함하는 키트
US11931674B2 (en) 2019-04-04 2024-03-19 Natera, Inc. Materials and methods for processing blood samples
US20220333183A1 (en) * 2019-10-02 2022-10-20 Rutgers, The State University Of New Jersey Assay methods and kits for detecting rare sequence variants
AU2021264510A1 (en) * 2020-04-28 2022-12-01 Cepheid Pseudo-complementary bases in genotyping and nucleic acid sequencing
WO2023013421A1 (ja) * 2021-08-03 2023-02-09 国立大学法人群馬大学 腫瘍マーカーca19-9によるがん診断の精度向上を目的とする血液型関連糖鎖遺伝子の迅速スクリーニングのための新規手法

Family Cites Families (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
CA1202962A (en) 1982-10-19 1986-04-08 David P. Clifford Substituted n-phenyl-n'-benzoyl ureas and their use as insecticides and acaricides
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5310893A (en) 1986-03-31 1994-05-10 Hoffmann-La Roche Inc. Method for HLA DP typing
US5604099A (en) 1986-03-13 1997-02-18 Hoffmann-La Roche Inc. Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids
CA1284931C (en) 1986-03-13 1991-06-18 Henry A. Erlich Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids
US4851331A (en) 1986-05-16 1989-07-25 Allied Corporation Method and kit for polynucleotide assay including primer-dependant DNA polymerase
US5322770A (en) 1989-12-22 1994-06-21 Hoffman-Laroche Inc. Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
US5561058A (en) 1986-08-22 1996-10-01 Hoffmann-La Roche Inc. Methods for coupled high temperatures reverse transcription and polymerase chain reactions
US5693517A (en) 1987-06-17 1997-12-02 Roche Molecular Systems, Inc. Reagents and methods for coupled high temperature reverse transcription and polymerase chain reactions
US5310652A (en) 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
IE61148B1 (en) 1988-03-10 1994-10-05 Ici Plc Method of detecting nucleotide sequences
US5639611A (en) 1988-12-12 1997-06-17 City Of Hope Allele specific polymerase chain reaction
US5137806A (en) 1989-12-11 1992-08-11 Board Of Regents, The University Of Texas System Methods and compositions for the detection of sequences in selected DNA molecules
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
DE69128520T2 (de) 1990-10-31 1998-07-09 Tosoh Corp Verfahren zum Nachweis oder Quantifizierung von Zielnukleinsäuren
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5539082A (en) 1993-04-26 1996-07-23 Nielsen; Peter E. Peptide nucleic acids
DK51092D0 (da) 1991-05-24 1992-04-15 Ole Buchardt Oligonucleotid-analoge betegnet pna, monomere synthoner og fremgangsmaade til fremstilling deraf samt anvendelser deraf
CA2081582A1 (en) 1991-11-05 1993-05-06 Teodorica Bugawan Methods and reagents for hla class i dna typing
JP2001507921A (ja) 1992-04-27 2001-06-19 トラスティーズ オブ ダートマス カレッジ 生物学的液体中の遺伝子配列の検出
CA2126952C (en) 1994-06-28 2010-12-14 Sithian Pandian Probe, kit, and method of amplification for increasing the sensitivity of nucleic acid hybridization assays
US6235480B1 (en) 1998-03-13 2001-05-22 Promega Corporation Detection of nucleic acid hybrids
US20030211483A1 (en) 2002-05-09 2003-11-13 Schroeder Benjamin G. Methods for the enrichment of low-abundance polynucleotides
WO2003095645A1 (en) 2002-05-09 2003-11-20 New York University Assay for melanogenesis
WO2005027710A2 (en) 2002-09-11 2005-03-31 Sequenom, Inc. Methods for identifying subjects at risk of melanoma and treatments thereof
EP1546393A2 (en) 2002-09-26 2005-06-29 Novartis AG Pcr-based diagnostic method of detecting a mutation in the b-raf gene
JP4116856B2 (ja) 2002-10-02 2008-07-09 富士フイルム株式会社 1塩基多型の検出方法
US20040121374A1 (en) 2002-10-02 2004-06-24 Yoshihide Iwaki Method for detecting single nucleotide polymorphisms
US7250289B2 (en) * 2002-11-20 2007-07-31 Affymetrix, Inc. Methods of genetic analysis of mouse
US7378233B2 (en) 2003-04-12 2008-05-27 The Johns Hopkins University BRAF mutation T1796A in thyroid cancers
EP1709200B1 (en) 2003-12-03 2012-05-09 Abbott Laboratories Double stranded linear nucleic acid probe and uses thereof
EP1541695A1 (en) 2003-12-09 2005-06-15 Nanogen Recognomics GmbH Use of a mutation in the BRAF gene for the determination of the malignancy of melanoma cells
WO2005066346A1 (ja) 2003-12-26 2005-07-21 Takeshi Nagasaka 核酸増幅用プライマー、核酸増幅用プライマーセット及びこれを用いた癌の検査方法
ES2244308B1 (es) 2004-01-21 2007-02-16 Oryzon Genomics, S.A. Metodo de diagnostico molecular y tratamiento en cancer colorrectal esporadico inestable.
US7442507B2 (en) 2005-01-24 2008-10-28 New York University School Of Medicine Methods for detecting circulating mutant BRAF DNA
US20070020657A1 (en) 2005-05-20 2007-01-25 Grebe Stefan K Methods for detecting circulating tumor cells
DK2395004T3 (en) * 2005-06-22 2016-03-21 Plexxikon Inc Pyrrolo [2,3-b] pyridine derivatives as protein kinase inhibitors
WO2007009013A2 (en) 2005-07-13 2007-01-18 University Of Utah Research Foundation Methods and compositions related to a braf mutation and microsatellite stability
US20100009355A1 (en) 2006-03-14 2010-01-14 Kolodney Michael S Selective amplification of minority mutations using primer blocking high-affinity oligonucleotides
US7803543B2 (en) * 2007-01-19 2010-09-28 Chang Gung University Methods and kits for the detection of nucleotide mutations using peptide nucleic acid as both PCR clamp and sensor probe
EP2129791A4 (en) 2007-02-26 2010-07-28 Wayne John Cancer Inst BENEFITS OF THE B-RAF DNA MUTATION FOR THE DIAGNOSIS AND TREATMENT OF CANCER
US20100173294A1 (en) 2007-09-11 2010-07-08 Roche Molecular Systems, Inc. Diagnostic test for susceptibility to b-raf kinase inhibitors
US20110212991A1 (en) 2007-09-11 2011-09-01 Roche Molecular Systems, Inc. Diagnostic Test for Susceptibility to B-RAF Kinase Inhibitors
WO2009073513A1 (en) 2007-11-30 2009-06-11 Schering Corporation Braf biomarkers
WO2010011884A2 (en) 2008-07-25 2010-01-28 University Of Florida Research Foundation, Inc. Novel nucleic acid-based molecular probes
US8583380B2 (en) 2008-09-05 2013-11-12 Aueon, Inc. Methods for stratifying and annotating cancer drug treatment options
CN101487051B (zh) 2009-02-24 2011-07-20 广州益善生物技术有限公司 Braf基因突变的检测探针、液相芯片及其检测方法
US8728763B2 (en) * 2009-08-11 2014-05-20 Response Genetics Methods, primers, probes and kits useful for the detection of BRAF mutations
CA2770716A1 (en) 2009-08-11 2011-02-17 Response Genetics, Inc. Methods, primers, probes and kits useful for the detection of braf mutations
KR101825117B1 (ko) 2010-02-01 2018-02-06 주식회사 파나진 Pna 기반의 실시간 pcr 클램핑을 이용한 braf 돌연변이 검출 방법 및 키트
CN102161990A (zh) * 2010-02-24 2011-08-24 北京雅康博生物科技有限公司 用于定量检测braf突变的试剂盒
EP3028699B1 (en) 2010-02-25 2018-03-21 Dana-Farber Cancer Institute, Inc. Braf mutations conferring resistance to braf inhibitors
WO2011104694A2 (en) 2010-02-26 2011-09-01 GAMMAGENETICS Sàrl Detection of braf v600e mutation by allele specific real time quantitative pcr (as-qpcr) using locked nucleic acids primers and beacon probes
US20110236916A1 (en) 2010-03-26 2011-09-29 Mayo Foundation For Medical Education And Research Methods and materials for detecting colorectal neoplasm
CN102234684B (zh) 2010-04-23 2014-01-01 益善生物技术股份有限公司 Braf基因突变检测特异性引物和液相芯片
EP2426217A1 (en) 2010-09-03 2012-03-07 Centre National de la Recherche Scientifique (CNRS) Analytical methods for cell free nucleic acids and applications

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Publication number Publication date
EP2855708A2 (en) 2015-04-08
WO2013181125A9 (en) 2014-07-17
US20130323727A1 (en) 2013-12-05
EP2855708B1 (en) 2019-07-24
CA2875281A1 (en) 2013-12-05
WO2013181125A2 (en) 2013-12-05
JP2018196379A (ja) 2018-12-13
JP2015518727A (ja) 2015-07-06
EP3604552A1 (en) 2020-02-05
AU2013267660B2 (en) 2018-07-19
AU2018250508A1 (en) 2018-11-15
JP6448534B2 (ja) 2019-01-09
US10077474B2 (en) 2018-09-18
EP2855708A4 (en) 2016-01-20
US20190032133A1 (en) 2019-01-31
WO2013181125A3 (en) 2014-01-30
AU2013267660A1 (en) 2015-01-22

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