ES2676895T3 - Variantes combinatorias de alfa-amilasa - Google Patents
Variantes combinatorias de alfa-amilasa Download PDFInfo
- Publication number
- ES2676895T3 ES2676895T3 ES14722824T ES14722824T ES2676895T3 ES 2676895 T3 ES2676895 T3 ES 2676895T3 ES 14722824 T ES14722824 T ES 14722824T ES 14722824 T ES14722824 T ES 14722824T ES 2676895 T3 ES2676895 T3 ES 2676895T3
- Authority
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- seq
- amino acid
- amylase
- numbering
- mutation
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Links
- 102000004139 alpha-Amylases Human genes 0.000 title abstract 8
- 108090000637 alpha-Amylases Proteins 0.000 title abstract 8
- 229940024171 alpha-amylase Drugs 0.000 title abstract 8
- 230000035772 mutation Effects 0.000 abstract 7
- 125000000539 amino acid group Chemical group 0.000 abstract 6
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 3
- 229920002472 Starch Polymers 0.000 abstract 1
- 238000004140 cleaning Methods 0.000 abstract 1
- 239000003599 detergent Substances 0.000 abstract 1
- 235000019698 starch Nutrition 0.000 abstract 1
- 239000008107 starch Substances 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
- A23L2/382—Other non-alcoholic beverages fermented
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38681—Chemically modified or immobilised enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Enzymes And Modification Thereof (AREA)
- Detergent Compositions (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Variante recombinante de una α-amilasa original que comprende: i) una mutación en un residuo de aminoácido E187 utilizando SEQ ID N.º 1 para la numeración; y al menos una mutación en un residuo de aminoácido seleccionado del grupo que consiste en N126, Y150, F153, L171, T180, y 1203 utilizando SEQ ID N.º 1 para la numeración; donde la variante de α-amilasa presenta al menos un 70 % de identidad de secuencia de aminoácidos con respecto a SEQ ID N.º 1; o ii) una mutación en un residuo de aminoácido E186 utilizando SEQ ID N.º 3 para la numeración; y al menos una mutación en un residuo de aminoácido seleccionado del grupo que consiste en N125, Y149, F152, L170, D179, y L202 utilizando SEQ ID N.º 3 para la numeración; donde la variante de α-amilasa presenta al menos un 70 % de identidad de secuencia de aminoácidos con respecto a SEQ ID N.º 3; o iii) una mutación en un residuo de aminoácido E189 utilizando SEQ ID N.º 5 para la numeración; y al menos una mutación en un residuo de aminoácido seleccionado del grupo que consiste en N128, Y152, F155, L173, T182, y L205 utilizando SEQ ID N.º 5 para la numeración; donde la variante de α-amilasa presenta al menos un 70 % de identidad de secuencia de aminoácidos con respecto a SEQ ID N.º 5; donde la variante presenta un aumento de la estabilidad térmica, estabilidad detergente, actividad de licuefacción de almidón, y/o rendimiento de limpieza en comparación con la α-amilasa original o una α- amilasa de referencia que difiera de la variante de α-amilasa únicamente por la ausencia de las mutaciones.
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201361776699P | 2013-03-11 | 2013-03-11 | |
US201361906617P | 2013-11-20 | 2013-11-20 | |
US201361907131P | 2013-11-21 | 2013-11-21 | |
PCT/US2014/023458 WO2014164777A1 (en) | 2013-03-11 | 2014-03-11 | Alpha-amylase combinatorial variants |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2676895T3 true ES2676895T3 (es) | 2018-07-26 |
ES2676895T5 ES2676895T5 (es) | 2022-04-27 |
Family
ID=50680117
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES14722824T Active ES2676895T5 (es) | 2013-03-11 | 2014-03-11 | Variantes combinatorias de alfa-amilasa |
ES18151284T Active ES2882517T3 (es) | 2013-03-11 | 2014-03-11 | Variantes combinatorias de alfa-amilasa |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES18151284T Active ES2882517T3 (es) | 2013-03-11 | 2014-03-11 | Variantes combinatorias de alfa-amilasa |
Country Status (9)
Country | Link |
---|---|
US (5) | US20160017304A1 (es) |
EP (5) | EP2970931B1 (es) |
CN (3) | CN110777132B (es) |
BR (1) | BR112015021647B1 (es) |
CA (1) | CA2903027A1 (es) |
DK (3) | DK3336183T3 (es) |
ES (2) | ES2676895T5 (es) |
HU (1) | HUE039341T2 (es) |
WO (3) | WO2014164800A1 (es) |
Families Citing this family (161)
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EP2935575B1 (en) * | 2012-12-21 | 2018-04-18 | Danisco US Inc. | Alpha-amylase variants |
US10538751B2 (en) * | 2013-06-06 | 2020-01-21 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
US20180112204A1 (en) | 2015-05-13 | 2018-04-26 | Danisco Us Inc. | AprL-CLADE PROTEASE VARIANTS AND USES THEREOF |
WO2016201040A1 (en) | 2015-06-09 | 2016-12-15 | Danisco Us Inc. | Water-triggered enzyme suspension |
WO2016201069A1 (en) | 2015-06-09 | 2016-12-15 | Danisco Us Inc | Low-density enzyme-containing particles |
DK3307427T3 (da) | 2015-06-09 | 2023-11-06 | Danisco Us Inc | Osmotisk sprængnings-kapsler |
EP3310911B1 (en) | 2015-06-17 | 2023-03-15 | Danisco US Inc. | Bacillus gibsonii-clade serine proteases |
DK3368655T3 (da) | 2015-10-30 | 2020-09-14 | Danisco Us Inc | Forbedret proteinekspression og fremgangsmåder dertil |
EP4141113A1 (en) | 2015-11-05 | 2023-03-01 | Danisco US Inc | Paenibacillus sp. mannanases |
JP7364330B2 (ja) | 2015-11-05 | 2023-10-18 | ダニスコ・ユーエス・インク | パエニバチルス(Paenibacillus)属種及びバチルス(Bacillus)属種のマンナナーゼ |
CN108779448B (zh) * | 2015-12-09 | 2023-08-18 | 丹尼斯科美国公司 | α-淀粉酶组合变体 |
BR112018012020A2 (pt) | 2015-12-18 | 2018-12-04 | Danisco Us Inc | polipeptídeos com atividade de endoglucanase e usos dos mesmos |
CN108699579A (zh) * | 2015-12-31 | 2018-10-23 | Cj第制糖株式会社 | 使用具有耐热性及耐酸性的酸热菌菌种衍生的脱支酶从淀粉糖制备葡萄糖的方法以及由此制备的葡萄糖 |
DK179660B1 (en) | 2016-04-08 | 2019-03-13 | Novozymes A/S | Stabilized Alpha-Amylase Variants and use of the same |
CA3022875A1 (en) | 2016-05-03 | 2017-11-09 | Danisco Us Inc | Protease variants and uses thereof |
CN109072213A (zh) | 2016-05-05 | 2018-12-21 | 丹尼斯科美国公司 | 蛋白酶变体及其用途 |
US11661567B2 (en) | 2016-05-31 | 2023-05-30 | Danisco Us Inc. | Protease variants and uses thereof |
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- 2014-03-11 EP EP14727646.3A patent/EP2970931B1/en active Active
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