ES2535047T3 - Método para la desparafinación de una muestra tisular a base de una microemulsión - Google Patents
Método para la desparafinación de una muestra tisular a base de una microemulsión Download PDFInfo
- Publication number
- ES2535047T3 ES2535047T3 ES05824805.5T ES05824805T ES2535047T3 ES 2535047 T3 ES2535047 T3 ES 2535047T3 ES 05824805 T ES05824805 T ES 05824805T ES 2535047 T3 ES2535047 T3 ES 2535047T3
- Authority
- ES
- Spain
- Prior art keywords
- microemulsion
- dewaxing
- paraffin
- tissue sample
- sample based
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000004530 micro-emulsion Substances 0.000 title abstract 4
- 239000012188 paraffin wax Substances 0.000 abstract 3
- 239000012472 biological sample Substances 0.000 abstract 2
- 239000003495 polar organic solvent Substances 0.000 abstract 2
- 239000004094 surface-active agent Substances 0.000 abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 2
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Extraction Or Liquid Replacement (AREA)
Abstract
Procedimiento de retirada del medio impregnado en parafina de una muestra biológica impregnada en parafina que comprende: poner en contacto la muestra biológica impregnada de parafina con una microemulsión desparafinante que comprende tensoactivo, solvente orgánico no polar y agua, en la que el tensoactivo es soluble tanto en agua como en el solvente orgánico no polar, transfiriendo la parafina a la microemulsión; y Separar la microemulsión.
Description
Claims (1)
-
imagen1
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US63694204P | 2004-12-17 | 2004-12-17 | |
US636942P | 2004-12-17 | ||
PCT/US2005/041853 WO2006065442A2 (en) | 2004-12-17 | 2005-11-18 | Methods and compositions for a microemulsion-based tissue treatment |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2535047T3 true ES2535047T3 (es) | 2015-05-04 |
Family
ID=36084210
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES05824805.5T Active ES2535047T3 (es) | 2004-12-17 | 2005-11-18 | Método para la desparafinación de una muestra tisular a base de una microemulsión |
Country Status (8)
Country | Link |
---|---|
US (4) | US20060134732A1 (es) |
EP (5) | EP2390648A1 (es) |
JP (4) | JP5137585B2 (es) |
AU (1) | AU2005316930B2 (es) |
CA (5) | CA2764289C (es) |
DK (2) | DK2392908T3 (es) |
ES (1) | ES2535047T3 (es) |
WO (1) | WO2006065442A2 (es) |
Families Citing this family (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2424988T3 (es) | 2002-04-15 | 2013-10-10 | Ventana Medical Systems, Inc. | Sistema automatizado de coloración de placas portaobjetos con elevada velocidad de producción |
US11249095B2 (en) | 2002-04-15 | 2022-02-15 | Ventana Medical Systems, Inc. | Automated high volume slide processing system |
US7468161B2 (en) * | 2002-04-15 | 2008-12-23 | Ventana Medical Systems, Inc. | Automated high volume slide processing system |
EP2390648A1 (en) * | 2004-12-17 | 2011-11-30 | Ventana Medical Systems, Inc. | Methods and compositions for a microemulsion-based tissue treatment |
WO2006116199A2 (en) | 2005-04-21 | 2006-11-02 | Celerus Diagnostics, Inc. | Parallel processing fluidic method and apparatus for automated rapid immunohistochemistry |
WO2007084429A2 (en) * | 2006-01-13 | 2007-07-26 | Ventana Medical Systems, Inc. | Biological sample processing composition and method |
US20100075372A1 (en) * | 2006-09-28 | 2010-03-25 | Taka-Aki Sato | Method for deparaffinization of paraffin-embedded specimen and method for analysis of paraffin-embedded specimen |
CN102209889B (zh) | 2008-11-12 | 2015-05-13 | 文塔纳医疗系统公司 | 用于加热载持标本的载玻片的方法及设备 |
EP2468862A1 (en) * | 2010-12-23 | 2012-06-27 | Qiagen GmbH | Method and kit for processing wax-embedded biological samples |
CN103930761B (zh) * | 2011-02-28 | 2017-06-13 | 丹麦达科有限公司 | 用于预处理包埋的生物样本的不混溶两相系统 |
DE102011002197B4 (de) * | 2011-04-20 | 2019-03-07 | Leica Biosystems Nussloch Gmbh | Verfahren und Vorrichtung zum Ablösen und/oder Vereinzeln einer histologischen Probe |
WO2013079072A1 (en) * | 2011-11-30 | 2013-06-06 | Dako Denmark A/S | Method and system for automated deparaffinization and non-immunohistochemical special staining of tissue samples |
US8753836B2 (en) * | 2012-01-23 | 2014-06-17 | Richard-Allan Scientific Company | Dewaxing buffer containing a water-soluble organic solvent and methods of use thereof |
EP2873028A4 (en) * | 2012-06-28 | 2016-05-25 | Pelican Imaging Corp | SYSTEMS AND METHOD FOR DETECTING DEFECTIVE CAMERA ARRAYS, OPTICAL ARRAYS AND SENSORS |
JP6253657B2 (ja) | 2012-10-08 | 2017-12-27 | ヴェンタナ メディカル システムズ, インク. | 有色素サンプルを明確化するための方法、キット、及びシステム |
US9080167B2 (en) * | 2012-11-16 | 2015-07-14 | Covaris, Inc. | System and method for processing paraffin embedded samples |
WO2014079802A2 (en) | 2012-11-20 | 2014-05-30 | Ventana Medical Systems, Inc. | Laser ablation inductively-coupled plasma mass spectral tissue diagnostics |
AU2014363717B2 (en) | 2013-12-13 | 2016-12-22 | Ventana Medical Systems, Inc. | Automated histological processing of biological specimens and associated technology |
WO2017158469A1 (en) | 2016-03-15 | 2017-09-21 | Sabic Global Technologies B.V. | Methods of making alpha, beta-unsaturated carboxylic acids or salts |
CN106770226A (zh) * | 2016-11-29 | 2017-05-31 | 河南中医药大学 | 一种用于观察植物花药发育结构变化的染色方法 |
US10852219B2 (en) | 2017-11-02 | 2020-12-01 | Diagnostic Biosystems | Deparaffinizing compositions and methods |
EP4211445A1 (en) | 2020-09-08 | 2023-07-19 | Clarapath, Inc. | Automated tissue section transfer system with high throughput |
JP7309140B1 (ja) | 2022-04-07 | 2023-07-18 | 学校法人昭和大学 | 顕微鏡標本作製用平板及び顕微鏡標本の作製方法 |
DE102022111890B3 (de) | 2022-05-12 | 2023-03-16 | Dermagnostix GmbH | Verfahren zur Deparaffinierung von Formalin-fixiertem Paraffin-eingebettetem Gewebe |
Family Cites Families (65)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2681298A (en) * | 1951-01-27 | 1954-06-15 | Technicon Chemical Company Inc | Preparation for and method of treating histological tissue |
US3546334A (en) * | 1965-05-21 | 1970-12-08 | Lerner Lab Inc | Composition for fixing and protecting a smear of body cells and method of applying same |
US3624197A (en) * | 1969-09-04 | 1971-11-30 | Philip Schain | Water-soluble tissue infiltrating and embedding compositions |
US3761401A (en) * | 1971-04-26 | 1973-09-25 | Marathon Oil Co | Hydrocarbon extraction process using micellar systems |
CA1041382A (en) * | 1974-11-08 | 1978-10-31 | Vincent N.R. Sewell | Method of enhancing oleophilic and hydrophobic properties of absorbent material |
DE2826363C2 (de) * | 1978-06-16 | 1984-08-16 | Merck Patent Gmbh, 6100 Darmstadt | Deckfolie zur Verwendung in mikroskopischen Färbeverfahren und Verfahren zu ihrer Herstellung |
US4402708A (en) * | 1980-11-18 | 1983-09-06 | Exxon Research & Engineering Co. | Dialkyl amine derivatives of phthalic acid |
JPS57111399A (en) * | 1980-11-19 | 1982-07-10 | Du Pont | Emulsion for aqueous detergent |
US4446044A (en) * | 1981-04-09 | 1984-05-01 | E. I. Du Pont De Nemours And Company | Aqueous water-in-oil cleaning emulsion |
US4404181A (en) * | 1981-10-15 | 1983-09-13 | Cambridge Chemical Products, Inc. | Extended-life tissue fixative composition and method of using the same |
US4526586A (en) * | 1982-09-24 | 1985-07-02 | The United States Of America As Represented By The Secretary Of Agriculture | Microemulsions from vegetable oil and aqueous alcohol with 1-butanol surfactant as alternative fuel for diesel engines |
US4557734A (en) * | 1984-08-08 | 1985-12-10 | The United States Of America As Represented By The Secretary Of Agriculture | Microemulsions from vegetable oil and lower alcohol with octanol surfactant as alternative fuel for diesel engines |
US4946669A (en) * | 1987-10-09 | 1990-08-07 | Siegfried Barry A | Histological fixatives |
US5130238A (en) * | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
US5219560A (en) * | 1989-03-20 | 1993-06-15 | Kobayashi Kose Co., Ltd. | Cosmetic composition |
US5106730A (en) * | 1989-07-24 | 1992-04-21 | Microprobe Corporation | Lactam-containing compositions and methods useful for the hybridization of nucleic acids |
US5124444A (en) * | 1989-07-24 | 1992-06-23 | Microprobe Corporation | Lactam-containing compositions and methods useful for the extraction of nucleic acids |
JPH0376797A (ja) * | 1989-08-21 | 1991-04-02 | Lion Corp | 液体洗浄剤組成物 |
US5521061A (en) * | 1992-07-17 | 1996-05-28 | Aprogenex, Inc. | Enhancement of probe signal in nucleic acid-mediated in-situ hybridization studies |
US5492837A (en) * | 1993-08-27 | 1996-02-20 | Biogenex Laboratories | Mounting medium for microscope slide preparations |
US5552087A (en) * | 1993-11-15 | 1996-09-03 | Ventana Medical Systems, Inc. | High temperature evaporation inhibitor liquid |
US6632598B1 (en) | 1994-03-11 | 2003-10-14 | Biogenex Laboratories | Deparaffinization compositions and methods for their use |
US6451551B1 (en) * | 1994-03-11 | 2002-09-17 | Biogenex Laboratories | Releasing embedding media from tissue specimens |
JPH0841263A (ja) * | 1994-08-01 | 1996-02-13 | Nisshin Kaken Kk | 塩素化ポリプロピレンエマルションの製造方法 |
JP3522914B2 (ja) * | 1994-08-09 | 2004-04-26 | ヤスハラケミカル株式会社 | 病理組織検査用溶剤 |
WO1997016731A1 (en) * | 1995-11-02 | 1997-05-09 | Thomas Jefferson University | Immunocytochemical staining of exfoliated cervical samples |
JPH09157698A (ja) * | 1995-12-05 | 1997-06-17 | Olympus Optical Co Ltd | 洗浄組成物及び洗浄方法 |
US5866748A (en) * | 1996-04-23 | 1999-02-02 | Exxon Research And Engineering Company | Hydroisomerization of a predominantly N-paraffin feed to produce high purity solvent compositions |
JP3742464B2 (ja) * | 1996-08-09 | 2006-02-01 | ヤスハラケミカル株式会社 | プレパラート用封入剤 |
US5856289A (en) * | 1996-12-02 | 1999-01-05 | Kennedy; Michael Stephen | Hard surface cleaner with d-limonene, ethylene slycolbutyl ether, nonylphenol ethoxylate, and phosphate |
CA2235358A1 (en) * | 1997-04-21 | 1998-10-21 | Masahiro Takahashi | Surface modifier composition |
WO1999000470A1 (en) * | 1997-06-30 | 1999-01-07 | The Procter & Gamble Company | LIGHT-DUTY LIQUID OR GEL DISHWASHING DETERGENT COMPOSITIONS HAVING CONTROLLED pH AND DESIRABLE FOOD SOIL REMOVAL, RHEOLOGICAL AND SUDSING CHARACTERISTICS |
PT1000138E (pt) * | 1997-07-31 | 2004-01-30 | Du Pont | Microemulsoes aquosas |
DK1005633T3 (da) * | 1997-08-20 | 2008-12-15 | Univ Miami | Vævsfikserings-dehydratiserings-fedtfjernelses-imprægneringsmetode med höj kvalitet og kontinuerligt gennemlöb |
US6124253A (en) * | 1997-09-16 | 2000-09-26 | Church & Dwight Co., Inc. | Aqueous composition for low-temperature metal-cleaning and method of use |
US6649368B1 (en) * | 1997-10-24 | 2003-11-18 | Cell Marque Corporation | Composition and method for treating tissue samples |
NZ506722A (en) * | 1998-02-24 | 2002-10-25 | Weyerhaeuser Co | Method for removing wax from a pulp furnish |
EP1082611A4 (en) | 1998-06-02 | 2003-01-15 | Ventana Med Syst Inc | METHODS OF COLORING BIOLOGICAL SAMPLES BY COMBINING REAGENTS INSTABLE ON THE OBJECT BLADE |
US6143512A (en) * | 1998-08-17 | 2000-11-07 | Markovic; Nenad | Cap-pap test |
US7410753B2 (en) * | 1998-09-03 | 2008-08-12 | Ventana Medical Systems, Inc. | Removal of embedding media from biological samples and cell conditioning on automated staining instruments |
JP3782663B2 (ja) * | 1998-09-03 | 2006-06-07 | ベンタナ・メデイカル・システムズ・インコーポレーテツド | 生物学的試料からの包埋媒体の除去および自動化された染色装置における細胞コンディショニング |
US6255393B1 (en) * | 1998-10-22 | 2001-07-03 | Nippon Shokubai Co., Ltd. | Aqueous polymer dispersion, production process therefor, and aqueous release agent |
US6013683A (en) * | 1998-12-17 | 2000-01-11 | Dow Corning Corporation | Single phase silicone and water compositions |
US6544798B1 (en) * | 1999-02-26 | 2003-04-08 | Ventana Medical Systems, Inc. | Removal of embedding media from biological samples and cell conditioning on automated staining instruments |
JP2000258318A (ja) * | 1999-03-09 | 2000-09-22 | Muto Kagaku Kk | 溶媒置換用組成物及びこれを用いた標本作成方法 |
US6916608B2 (en) * | 1999-09-10 | 2005-07-12 | Becton, Dickinson And Company | Composition for providing long term stability to cells for diagnostic testing |
JP2001125006A (ja) * | 1999-10-27 | 2001-05-11 | Muto Kagaku Kk | 封入剤を印刷したカバーガラス及びその製造方法 |
US6337189B1 (en) * | 2000-02-08 | 2002-01-08 | Streck Laboratories, Inc. | Fixative system, method and composition for biological testing |
EP1325300A1 (en) * | 2000-09-15 | 2003-07-09 | Biogenex Laboratories | ENHANCEMENT OF i IN SITU /i HYBRIDIZATION |
DE60043845D1 (de) * | 2000-10-02 | 2010-04-01 | Mallinckrodt Baker B V | Verwendung eines verbesserten Befestigungsmittels für Objektträger |
FR2819201B1 (fr) * | 2001-01-09 | 2003-02-21 | Atofina | Procede de nettoyage d'une surface solide par elimination de salissures organiques et/ou minerales au moyen d'une microemulsion |
JP4801841B2 (ja) * | 2001-02-19 | 2011-10-26 | 東レ東燃機能膜合同会社 | 熱可塑性樹脂微多孔膜の洗浄方法およびこれを利用した熱可塑性樹脂微多孔膜の製造方法 |
DE10144989B4 (de) * | 2001-09-12 | 2007-11-08 | Leica Microsystems Nussloch Gmbh | System zum Färben und Eindecken von Objektträgern |
US6891502B2 (en) * | 2002-01-10 | 2005-05-10 | Mems Optical, Inc. | Variable shape antenna etching system and antenna formed thereby |
US7468161B2 (en) * | 2002-04-15 | 2008-12-23 | Ventana Medical Systems, Inc. | Automated high volume slide processing system |
ES2424988T3 (es) * | 2002-04-15 | 2013-10-10 | Ventana Medical Systems, Inc. | Sistema automatizado de coloración de placas portaobjetos con elevada velocidad de producción |
ATE435418T1 (de) * | 2002-05-28 | 2009-07-15 | Diapath S P A | Zusammensetzung für die herstellung von histologischen, autopsischen und zytologischen proben |
US20040122390A1 (en) * | 2002-12-23 | 2004-06-24 | Kimberly-Clark Worldwide, Inc. | Low evaporative superabsorbent products and composites and methods for utilizing the same |
US6953507B2 (en) * | 2003-03-21 | 2005-10-11 | Ecolab Inc. | Low temperature cleaning |
US7186522B2 (en) * | 2003-03-31 | 2007-03-06 | Cytyc Corporation | Papanicolau staining process |
KR20060115366A (ko) * | 2003-10-24 | 2006-11-08 | 더 유니버시티 오브 마이애미 | 간소화시킨 조직 가공 |
KR100837041B1 (ko) | 2004-02-18 | 2008-06-11 | 신에쓰 가가꾸 고교 가부시끼가이샤 | 대형 합성 석영 유리 기판의 제조 방법 |
EP2390648A1 (en) * | 2004-12-17 | 2011-11-30 | Ventana Medical Systems, Inc. | Methods and compositions for a microemulsion-based tissue treatment |
WO2007084429A2 (en) * | 2006-01-13 | 2007-07-26 | Ventana Medical Systems, Inc. | Biological sample processing composition and method |
FR2901124B1 (fr) * | 2006-05-17 | 2008-06-27 | Oreal | Emulsion huile dans eau fine |
-
2005
- 2005-11-18 EP EP11006283A patent/EP2390648A1/en not_active Withdrawn
- 2005-11-18 CA CA2764289A patent/CA2764289C/en active Active
- 2005-11-18 ES ES05824805.5T patent/ES2535047T3/es active Active
- 2005-11-18 CA CA2764294A patent/CA2764294C/en active Active
- 2005-11-18 DK DK11006284.1T patent/DK2392908T3/da active
- 2005-11-18 CA CA 2764335 patent/CA2764335C/en active Active
- 2005-11-18 CA CA2764621A patent/CA2764621C/en active Active
- 2005-11-18 US US11/284,397 patent/US20060134732A1/en not_active Abandoned
- 2005-11-18 EP EP11006282A patent/EP2390647A1/en not_active Withdrawn
- 2005-11-18 EP EP05824805.5A patent/EP1825246B1/en active Active
- 2005-11-18 JP JP2007546680A patent/JP5137585B2/ja active Active
- 2005-11-18 CA CA2588783A patent/CA2588783C/en active Active
- 2005-11-18 WO PCT/US2005/041853 patent/WO2006065442A2/en active Application Filing
- 2005-11-18 EP EP11006285.8A patent/EP2392909B1/en active Active
- 2005-11-18 DK DK11006285.8T patent/DK2392909T3/en active
- 2005-11-18 EP EP11006284.1A patent/EP2392908B1/en active Active
- 2005-11-18 AU AU2005316930A patent/AU2005316930B2/en active Active
-
2008
- 2008-06-30 US US12/165,020 patent/US8288121B2/en active Active
-
2012
- 2012-06-28 JP JP2012145623A patent/JP2012215581A/ja active Pending
- 2012-10-04 US US13/644,655 patent/US8512978B2/en active Active
-
2013
- 2013-07-18 US US13/945,587 patent/US8652803B2/en active Active
-
2014
- 2014-05-27 JP JP2014109000A patent/JP6018122B2/ja active Active
- 2014-05-27 JP JP2014108977A patent/JP6018121B2/ja active Active
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ES2535047T3 (es) | Método para la desparafinación de una muestra tisular a base de una microemulsión | |
ES2484142T3 (es) | Diagnóstico molecular y clasificación del melanoma maligno | |
ES2563305T3 (es) | Dispositivo de colágeno y procedimiento de preparación del mismo | |
ES2539133T3 (es) | Dispositivo para mejorar la función de la válvula cardiaca | |
JP2008524584A5 (es) | ||
ES2525879T3 (es) | Procedimiento, aparato y sistema para la limpieza basada en dos disolventes de componentes de precisión | |
AR031630A1 (es) | Un metodo para tomar imagenes y cosechar celulas de una colonia microbiana en un dispositivo de cultivo de pelicula delgada | |
EP1580669A3 (en) | Dynamic reference repository | |
ES2650950T3 (es) | Un método para filtrar una muestra biológica | |
AR060894A1 (es) | Aparato basado en membrana y metodo asociado | |
GB2442402A (en) | Use of supercritical fluid to dry wafer and clean lens in immersion lithography | |
EP1954091A3 (en) | Efficient network hand-off utilizing stored beam-forming information | |
ES2655069T3 (es) | Nanopartículas que comprenden ligandos de ARN | |
DK1252324T3 (da) | Solventfri ekstraktionsfremgangsmåde | |
EP2026209A3 (en) | System and method for using a memory mapping function to map memory defects | |
EP2113747A3 (en) | Point of interest search device and point of interest search method | |
EP1557721A3 (en) | Immersion lithography fluids | |
EP1818795A3 (en) | Storage system, data processing method and storage apparatus | |
EP3510865A3 (en) | Cryopreservation of biological cells and tissues | |
EP1693843A3 (en) | Method for tuning write strategy parameters of an optical storage device, and system thereof | |
CL2011000393A1 (es) | Metodo para preparar una microesfera de liberacion sostenidaque comprende disolver la sustancia activa y un polimero biodegradable en solvente acuoso y no acuoso respectivamente, formar una emulsion entre ambas soluciones, adicionarle un cosolvente y agregarla a un solvente acuoso para formar la microesfera y remover el solvente organico. | |
AU1576401A (en) | Method and apparatus for screening chemical compounds | |
EP2437176A3 (en) | System and method of using an N-way cache | |
AR071605A1 (es) | Concentrado de proteina vegetal | |
EP2204194A3 (en) | Probe for a hair cell, and labelling method for a hair cell using the probe for a hair cell |