ES2533622T3 - Virus de la gripe recombinantes para vacunas y terapia génica - Google Patents
Virus de la gripe recombinantes para vacunas y terapia génica Download PDFInfo
- Publication number
- ES2533622T3 ES2533622T3 ES10011896.7T ES10011896T ES2533622T3 ES 2533622 T3 ES2533622 T3 ES 2533622T3 ES 10011896 T ES10011896 T ES 10011896T ES 2533622 T3 ES2533622 T3 ES 2533622T3
- Authority
- ES
- Spain
- Prior art keywords
- linked
- virus
- influenza
- vector
- cdna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 241000712461 unidentified influenza virus Species 0.000 title abstract description 25
- 229960005486 vaccine Drugs 0.000 title description 9
- 238000001415 gene therapy Methods 0.000 title description 5
- 206010022000 influenza Diseases 0.000 abstract description 26
- 239000002299 complementary DNA Substances 0.000 abstract description 22
- 239000013598 vector Substances 0.000 abstract description 21
- 230000035897 transcription Effects 0.000 abstract description 12
- 238000013518 transcription Methods 0.000 abstract description 12
- 230000005030 transcription termination Effects 0.000 abstract description 12
- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 8
- 229920001184 polypeptide Polymers 0.000 abstract description 6
- 102000004196 processed proteins & peptides Human genes 0.000 abstract description 6
- 108010064513 influenza virus polymerase basic protein 1 Proteins 0.000 abstract 1
- 241000700605 Viruses Species 0.000 description 60
- 108090000623 proteins and genes Proteins 0.000 description 44
- 210000004027 cell Anatomy 0.000 description 39
- 239000013612 plasmid Substances 0.000 description 34
- 102000004169 proteins and genes Human genes 0.000 description 33
- 241000724284 Peanut stunt virus Species 0.000 description 28
- 102000017143 RNA Polymerase I Human genes 0.000 description 27
- 108010013845 RNA Polymerase I Proteins 0.000 description 25
- 102000011931 Nucleoproteins Human genes 0.000 description 21
- 108010061100 Nucleoproteins Proteins 0.000 description 21
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 19
- 108010006232 Neuraminidase Proteins 0.000 description 19
- 230000015572 biosynthetic process Effects 0.000 description 18
- 102000005348 Neuraminidase Human genes 0.000 description 17
- 238000000034 method Methods 0.000 description 17
- 208000015181 infectious disease Diseases 0.000 description 15
- 230000003612 virological effect Effects 0.000 description 13
- 108020004414 DNA Proteins 0.000 description 12
- 101710118188 DNA-binding protein HU-alpha Proteins 0.000 description 10
- 101710144128 Non-structural protein 2 Proteins 0.000 description 10
- 101710199667 Nuclear export protein Proteins 0.000 description 10
- 238000004519 manufacturing process Methods 0.000 description 10
- 238000002474 experimental method Methods 0.000 description 9
- 230000010076 replication Effects 0.000 description 9
- 230000002441 reversible effect Effects 0.000 description 9
- 108020004459 Small interfering RNA Proteins 0.000 description 8
- 230000002458 infectious effect Effects 0.000 description 8
- 108700008625 Reporter Genes Proteins 0.000 description 7
- 240000001068 Thogoto virus Species 0.000 description 7
- 241001493065 dsRNA viruses Species 0.000 description 7
- 239000013613 expression plasmid Substances 0.000 description 7
- 238000001890 transfection Methods 0.000 description 7
- 108020004635 Complementary DNA Proteins 0.000 description 6
- 241000712431 Influenza A virus Species 0.000 description 6
- 239000002245 particle Substances 0.000 description 6
- 238000005215 recombination Methods 0.000 description 6
- 230000006798 recombination Effects 0.000 description 6
- 230000035772 mutation Effects 0.000 description 5
- 239000002773 nucleotide Substances 0.000 description 5
- 125000003729 nucleotide group Chemical group 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 239000006228 supernatant Substances 0.000 description 5
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 4
- 241000124008 Mammalia Species 0.000 description 4
- 101150105115 PA gene Proteins 0.000 description 4
- 108020000999 Viral RNA Proteins 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 101150036876 cre gene Proteins 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000003786 synthesis reaction Methods 0.000 description 4
- 108020004394 Complementary RNA Proteins 0.000 description 3
- 102100031780 Endonuclease Human genes 0.000 description 3
- 241000702191 Escherichia virus P1 Species 0.000 description 3
- 206010028980 Neoplasm Diseases 0.000 description 3
- 101710172711 Structural protein Proteins 0.000 description 3
- 108010067390 Viral Proteins Proteins 0.000 description 3
- 108010087302 Viral Structural Proteins Proteins 0.000 description 3
- 201000011510 cancer Diseases 0.000 description 3
- 239000003184 complementary RNA Substances 0.000 description 3
- 230000004927 fusion Effects 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 230000003834 intracellular effect Effects 0.000 description 3
- 108020004999 messenger RNA Proteins 0.000 description 3
- 210000004940 nucleus Anatomy 0.000 description 3
- 230000002829 reductive effect Effects 0.000 description 3
- 210000002845 virion Anatomy 0.000 description 3
- 208000030507 AIDS Diseases 0.000 description 2
- 241000238876 Acari Species 0.000 description 2
- 108010042407 Endonucleases Proteins 0.000 description 2
- 241000709661 Enterovirus Species 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 241000710198 Foot-and-mouth disease virus Species 0.000 description 2
- 101000834253 Gallus gallus Actin, cytoplasmic 1 Proteins 0.000 description 2
- 102000003886 Glycoproteins Human genes 0.000 description 2
- 108090000288 Glycoproteins Proteins 0.000 description 2
- 108060003393 Granulin Proteins 0.000 description 2
- 241000712464 Orthomyxoviridae Species 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000002238 attenuated effect Effects 0.000 description 2
- SQVRNKJHWKZAKO-UHFFFAOYSA-N beta-N-Acetyl-D-neuraminic acid Natural products CC(=O)NC1C(O)CC(O)(C(O)=O)OC1C(O)C(O)CO SQVRNKJHWKZAKO-UHFFFAOYSA-N 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 108091092328 cellular RNA Proteins 0.000 description 2
- 238000003776 cleavage reaction Methods 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 210000001163 endosome Anatomy 0.000 description 2
- 210000003527 eukaryotic cell Anatomy 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 230000003053 immunization Effects 0.000 description 2
- 229960003971 influenza vaccine Drugs 0.000 description 2
- 108700010900 influenza virus proteins Proteins 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 230000003389 potentiating effect Effects 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 238000012552 review Methods 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 238000010187 selection method Methods 0.000 description 2
- SQVRNKJHWKZAKO-OQPLDHBCSA-N sialic acid Chemical compound CC(=O)N[C@@H]1[C@@H](O)C[C@@](O)(C(O)=O)OC1[C@H](O)[C@H](O)CO SQVRNKJHWKZAKO-OQPLDHBCSA-N 0.000 description 2
- 238000011282 treatment Methods 0.000 description 2
- 230000029812 viral genome replication Effects 0.000 description 2
- 230000007919 viral pathogenicity Effects 0.000 description 2
- 241000712891 Arenavirus Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 241001493154 Bunyamwera virus Species 0.000 description 1
- 241000282832 Camelidae Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 108010035563 Chloramphenicol O-acetyltransferase Proteins 0.000 description 1
- 108091026890 Coding region Proteins 0.000 description 1
- 206010010099 Combined immunodeficiency Diseases 0.000 description 1
- 241000701022 Cytomegalovirus Species 0.000 description 1
- 238000012270 DNA recombination Methods 0.000 description 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
- 208000001490 Dengue Diseases 0.000 description 1
- 206010012310 Dengue fever Diseases 0.000 description 1
- 241000991587 Enterovirus C Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000711950 Filoviridae Species 0.000 description 1
- 241000710831 Flavivirus Species 0.000 description 1
- 208000007212 Foot-and-Mouth Disease Diseases 0.000 description 1
- 108700007698 Genetic Terminator Regions Proteins 0.000 description 1
- 241000711549 Hepacivirus C Species 0.000 description 1
- 208000005176 Hepatitis C Diseases 0.000 description 1
- 241000709721 Hepatovirus A Species 0.000 description 1
- 101000986595 Homo sapiens Ornithine transcarbamylase, mitochondrial Proteins 0.000 description 1
- XQFRJNBWHJMXHO-RRKCRQDMSA-N IDUR Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(I)=C1 XQFRJNBWHJMXHO-RRKCRQDMSA-N 0.000 description 1
- 101710128560 Initiator protein NS1 Proteins 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 101100343701 Mus musculus Loxl1 gene Proteins 0.000 description 1
- 101710144127 Non-structural protein 1 Proteins 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 208000000599 Ornithine Carbamoyltransferase Deficiency Disease Diseases 0.000 description 1
- 206010052450 Ornithine transcarbamoylase deficiency Diseases 0.000 description 1
- 208000035903 Ornithine transcarbamylase deficiency Diseases 0.000 description 1
- 102100028200 Ornithine transcarbamylase, mitochondrial Human genes 0.000 description 1
- 241000713112 Orthobunyavirus Species 0.000 description 1
- 101150103639 PB1 gene Proteins 0.000 description 1
- 241000150350 Peribunyaviridae Species 0.000 description 1
- 241000710778 Pestivirus Species 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 108010052160 Site-specific recombinase Proteins 0.000 description 1
- 101001039853 Sonchus yellow net virus Matrix protein Proteins 0.000 description 1
- 230000005867 T cell response Effects 0.000 description 1
- 101710137500 T7 RNA polymerase Proteins 0.000 description 1
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical class O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 1
- 108091023045 Untranslated Region Proteins 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 201000009628 adenosine deaminase deficiency Diseases 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 208000036556 autosomal recessive T cell-negative B cell-negative NK cell-negative due to adenosine deaminase deficiency severe combined immunodeficiency Diseases 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 201000007455 central nervous system cancer Diseases 0.000 description 1
- 208000025997 central nervous system neoplasm Diseases 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 210000001151 cytotoxic T lymphocyte Anatomy 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 208000025729 dengue disease Diseases 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 206010014599 encephalitis Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 238000010230 functional analysis Methods 0.000 description 1
- 238000001476 gene delivery Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000009643 growth defect Effects 0.000 description 1
- 208000002672 hepatitis B Diseases 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 238000003119 immunoblot Methods 0.000 description 1
- 230000002163 immunogen Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 229940031551 inactivated vaccine Drugs 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 208000037797 influenza A Diseases 0.000 description 1
- 208000037798 influenza B Diseases 0.000 description 1
- 208000037799 influenza C Diseases 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 210000003292 kidney cell Anatomy 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 201000004792 malaria Diseases 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 238000013160 medical therapy Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000009456 molecular mechanism Effects 0.000 description 1
- 201000006938 muscular dystrophy Diseases 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 201000011278 ornithine carbamoyltransferase deficiency Diseases 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 208000005814 piedra Diseases 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000010837 receptor-mediated endocytosis Effects 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 201000009862 superficial mycosis Diseases 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 230000005026 transcription initiation Effects 0.000 description 1
- 238000010361 transduction Methods 0.000 description 1
- 230000026683 transduction Effects 0.000 description 1
- 241000701161 unidentified adenovirus Species 0.000 description 1
- 241000701447 unidentified baculovirus Species 0.000 description 1
- 210000000605 viral structure Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
- A61P31/16—Antivirals for RNA viruses for influenza or rhinoviruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/525—Virus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/525—Virus
- A61K2039/5258—Virus-like particles
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16111—Influenzavirus A, i.e. influenza A virus
- C12N2760/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16111—Influenzavirus A, i.e. influenza A virus
- C12N2760/16123—Virus like particles [VLP]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16111—Influenzavirus A, i.e. influenza A virus
- C12N2760/16141—Use of virus, viral particle or viral elements as a vector
- C12N2760/16143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2810/00—Vectors comprising a targeting moiety
- C12N2810/50—Vectors comprising as targeting moiety peptide derived from defined protein
- C12N2810/60—Vectors comprising as targeting moiety peptide derived from defined protein from viruses
- C12N2810/6072—Vectors comprising as targeting moiety peptide derived from defined protein from viruses negative strand RNA viruses
- C12N2810/6081—Vectors comprising as targeting moiety peptide derived from defined protein from viruses negative strand RNA viruses rhabdoviridae, e.g. VSV
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Virology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Veterinary Medicine (AREA)
- Physics & Mathematics (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Pulmonology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12791299P | 1999-04-06 | 1999-04-06 | |
| US127912P | 1999-04-06 | ||
| US13283999P | 1999-05-06 | 1999-05-06 | |
| US132839P | 1999-05-06 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2533622T3 true ES2533622T3 (es) | 2015-04-13 |
Family
ID=26826080
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES07009953T Expired - Lifetime ES2373405T3 (es) | 1999-04-06 | 2000-04-05 | Virus de la gripe recombinantes para vacunas y terapia génica. |
| ES10011896.7T Expired - Lifetime ES2533622T3 (es) | 1999-04-06 | 2000-04-05 | Virus de la gripe recombinantes para vacunas y terapia génica |
| ES00920151T Expired - Lifetime ES2290024T3 (es) | 1999-04-06 | 2000-04-05 | Virus de la gripe recombinantes para vacunas y terapia genica. |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES07009953T Expired - Lifetime ES2373405T3 (es) | 1999-04-06 | 2000-04-05 | Virus de la gripe recombinantes para vacunas y terapia génica. |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES00920151T Expired - Lifetime ES2290024T3 (es) | 1999-04-06 | 2000-04-05 | Virus de la gripe recombinantes para vacunas y terapia genica. |
Country Status (19)
| Country | Link |
|---|---|
| EP (4) | EP1820853B1 (enExample) |
| JP (3) | JP5543048B2 (enExample) |
| KR (1) | KR100702275B1 (enExample) |
| CN (2) | CN1350578B (enExample) |
| AT (2) | ATE368729T1 (enExample) |
| AU (1) | AU4073300A (enExample) |
| BR (1) | BRPI0009580B8 (enExample) |
| CA (2) | CA2365526C (enExample) |
| CY (2) | CY1108520T1 (enExample) |
| DE (6) | DE122008000061I1 (enExample) |
| DK (3) | DK1820853T3 (enExample) |
| ES (3) | ES2373405T3 (enExample) |
| FR (1) | FR11C0027I2 (enExample) |
| HK (1) | HK1214298A1 (enExample) |
| IL (2) | IL145702A0 (enExample) |
| MX (1) | MXPA01010082A (enExample) |
| NL (2) | NL300364I2 (enExample) |
| PT (2) | PT1185615E (enExample) |
| WO (1) | WO2000060050A2 (enExample) |
Families Citing this family (83)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6544785B1 (en) | 1998-09-14 | 2003-04-08 | Mount Sinai School Of Medicine Of New York University | Helper-free rescue of recombinant negative strand RNA viruses |
| IL145702A0 (en) * | 1999-04-06 | 2002-07-25 | Wisconsin Alumni Res Found | Recombination influenza viruses for vaccines and gene theraphy |
| US8715940B2 (en) | 1999-04-06 | 2014-05-06 | Wisconsin Alumni Research Foundation | Method of making recombinant influenza virus |
| GB9916794D0 (en) * | 1999-07-16 | 1999-09-22 | Isis Innovation | In vitro virus reconstitution |
| DE122008000056I1 (de) * | 1999-07-14 | 2009-04-09 | Sinai School Medicine | In vitro-rekonstitution von segmentierten negativstrang-rna-viren |
| SI1317559T1 (sl) | 2000-04-28 | 2009-04-30 | St Jude Childrens Res Hospital | Sistem transfekcije dna za pripravo infektivne negativne verige virusne rna |
| KR100877019B1 (ko) | 2000-06-23 | 2009-01-07 | 와이어쓰 홀딩스 코포레이션 | 야생형 및 키메릭 인플루엔자 바이러스성입자(브이엘피)의 조립 |
| EP1862550A1 (en) * | 2000-06-23 | 2007-12-05 | Wyeth Holdings Corporation | Assembly of wild-type and chimeric influenza virus-like particles (VLPs) |
| US7211378B2 (en) | 2002-01-31 | 2007-05-01 | Wisconsin Alumni Research Foundation | Filovirus vectors and noninfectious filovirus-based particles |
| US7226774B2 (en) | 2002-02-13 | 2007-06-05 | Wisconsin Alumni Research Foundation | Signal for packaging of influenza virus vectors |
| ES2526191T3 (es) | 2002-04-26 | 2015-01-08 | Medimmune, Llc | Método para producir virus influenza B infeccioso en cultivo celular |
| US7465456B2 (en) | 2002-04-26 | 2008-12-16 | Medimmune, Llc | Multi plasmid system for the production of influenza virus |
| EA012965B1 (ru) * | 2003-05-28 | 2010-02-26 | Висконсин Эламни Рисёч Фаундэйшн | Композиция для получения реассортантного рекомбинантного вируса гриппа, способ получения указанного вируса и реассортантный рекомбинантный вирус гриппа |
| CA2529647C (en) | 2003-06-16 | 2013-08-13 | Medimmune Vaccines, Inc. | Influenza hemagglutinin and neuraminidase variants |
| JP4771959B2 (ja) | 2003-12-23 | 2011-09-14 | メディミューン，エルエルシー | インフルエンザウイルス作製のための多重プラスミド系 |
| FR2866031B1 (fr) * | 2004-02-11 | 2012-10-19 | David Francois Joseph Millet | Chimere de virus pathogene emergent, medicament la contenant et utilisation en therapie antivirale |
| US20080254065A1 (en) | 2004-03-09 | 2008-10-16 | Chiron Corporation | Influenza Virus Vaccines |
| CA2568015C (en) | 2004-05-25 | 2013-08-27 | Medimmune Vaccines, Inc. | Influenza hemagglutinin and neuraminidase variants |
| MX2007005818A (es) | 2004-11-19 | 2007-07-20 | Wisconsin Alumni Res Found | Vectores recombinantes de la influenza con unidades de transcripcion en tandem. |
| CN101128598B (zh) | 2004-12-08 | 2013-04-24 | 米迪缪尼有限公司 | 生产流感疫苗组合物的方法 |
| RU2435855C2 (ru) | 2004-12-24 | 2011-12-10 | Солвей Байолоджикалз Б.В. | Способ продуцирования репликативной частицы вируса гриппа, композиция клеток (варианты), композиция клеточной культуры и ее применение |
| EP2441471B1 (en) | 2005-03-08 | 2014-10-08 | MedImmune, LLC | Reassortant influenza viruses |
| US11707520B2 (en) | 2005-11-03 | 2023-07-25 | Seqirus UK Limited | Adjuvanted vaccines with non-virion antigens prepared from influenza viruses grown in cell culture |
| NZ567979A (en) | 2005-11-04 | 2012-02-24 | Novartis Vaccines & Diagnostic | Adjuvanted influenza vaccines including a cytokine-inducing agents which is an agonist of Toll-Like Receptor 7 |
| EP1945252B1 (en) * | 2005-11-04 | 2013-05-29 | Novartis Vaccines and Diagnostics S.r.l. | Vaccines comprising purified surface antigens prepared from influenza viruses grown in cell culture, adjuvanted with squalene |
| ATE539765T1 (de) | 2005-11-04 | 2012-01-15 | Novartis Vaccines & Diagnostic | Grippeimpfstoffe mit kombinationen aus teilchenförmigen adjuvantien und immunverstärkern |
| KR20080069232A (ko) | 2005-11-04 | 2008-07-25 | 노바티스 백신즈 앤드 다이아그노스틱스 에스.알.엘. | 스플리트 인플루엔자 백신에 대한 보조제로서 유리 수성상계면활성제를 갖는 에멀젼 |
| EA016217B1 (ru) | 2005-12-02 | 2012-03-30 | Маунт Синай Скул Оф Медсин | Химерные вирусы, представляющие неприродные поверхностные белки, и их применение |
| CA2640248A1 (en) | 2006-01-27 | 2007-08-02 | Novartis Vaccines And Diagnostics Gmbh & Co Kg. | Influenza vaccines containing hemagglutinin and matrix proteins |
| US20100068223A1 (en) | 2006-03-24 | 2010-03-18 | Hanno Scheffczik | Storage of Influenza Vaccines Without Refrigeration |
| EP2010557B1 (en) | 2006-03-31 | 2014-02-26 | Wisconsin Alumni Research Foundation | High titer recombinant influenza viruses for vaccines |
| US20090017444A1 (en) * | 2006-06-09 | 2009-01-15 | Wisconsin Alumni Research Foundation | Screening method for modulators of viral transcription or replication |
| GB0614460D0 (en) | 2006-07-20 | 2006-08-30 | Novartis Ag | Vaccines |
| WO2008133701A1 (en) | 2006-07-21 | 2008-11-06 | Medimmune, Llc. | Methods and compositions for increasing replication capacity of an influenza virus |
| WO2008021959A2 (en) | 2006-08-09 | 2008-02-21 | Medimmune Vaccines, Inc. | Influenza hemagglutinin and neuraminidase variants |
| EP2497495B3 (en) | 2006-09-11 | 2021-02-17 | Seqirus UK Limited | Making influenza virus vaccines without using eggs |
| EP2121011B1 (en) | 2006-12-06 | 2014-05-21 | Novartis AG | Vaccines including antigen from four strains of influenza virus |
| US8597661B2 (en) | 2007-05-04 | 2013-12-03 | Wisconsin Alumni Research Foundation | Neuraminidase-deficient live influenza vaccines |
| CA2690196A1 (en) | 2007-06-18 | 2008-12-24 | Medimmune, Llc | Influenza b viruses having alterations in the hemaglutinin polypeptide |
| US9474798B2 (en) | 2007-06-18 | 2016-10-25 | Wisconsin Alumni Research Foundation | Influenza M2 protein mutant viruses as live influenza attenuated vaccines |
| DK2185191T3 (da) | 2007-06-27 | 2012-12-03 | Novartis Ag | Influenzavacciner med lavt indhold af tilsætningsstoffer |
| EP2045323A1 (en) * | 2007-10-05 | 2009-04-08 | Avir Green Hills Biotechnology Research Development Trade Ag | Linear expression constructs for production of influenza virus particles |
| CN101497877B (zh) * | 2008-01-28 | 2012-09-05 | 河北摩百生物科技有限公司 | 流感全病毒减活疫苗重组体、其构建方法及应用 |
| AU2009227674C1 (en) | 2008-03-18 | 2015-01-29 | Seqirus UK Limited | Improvements in preparation of influenza virus vaccine antigens |
| JP2011527581A (ja) | 2008-07-11 | 2011-11-04 | メディミューン，エルエルシー | インフルエンザ赤血球凝集素およびノイラミニダーゼ変異体 |
| AU2009283557B2 (en) | 2008-08-18 | 2013-01-17 | Kitasato Daiichi Sankyo Vaccine Co., Ltd. | Avian influenza virus antigen, and booster immunization method for avian influenza vaccine in combination with mucosal adjuvant which is effective through oral administration |
| EP2233568A1 (en) | 2009-03-19 | 2010-09-29 | Avir Green Hills Biotechnology Research Development Trade AG | Novel method for generation of RNA virus |
| JP2012517416A (ja) | 2009-02-10 | 2012-08-02 | ノバルティス アーゲー | 増加した量のｈ３抗原を含むインフルエンザワクチン |
| ES2552383T3 (es) | 2009-02-10 | 2015-11-27 | Novartis Ag | Regímenes de vacuna de la gripe para cepas asociadas a pandemias |
| KR101825697B1 (ko) | 2009-02-10 | 2018-02-05 | 노파르티스 아게 | 감소된 양의 스쿠알렌을 포함하는 인플루엔자 백신 |
| CA2752205A1 (en) | 2009-02-12 | 2010-08-19 | Medimmune, Llc | Influenza hemagglutinin and neuraminidase variants |
| CN102548577A (zh) | 2009-04-27 | 2012-07-04 | 诺华有限公司 | 用于抵抗流感的佐剂疫苗 |
| BR112012008338A2 (pt) | 2009-09-10 | 2019-09-24 | Novartis Ag | combinação de vacinas contra doenças do trato respiratório. |
| JP2013507990A (ja) | 2009-10-26 | 2013-03-07 | ダブリュエーアールエフ−ウィスコンシン アラムナイ リサーチ ファウンデーション | ベロ細胞において増強された複製を有する高力価の組換えインフルエンザウイルス |
| US10130697B2 (en) | 2010-03-23 | 2018-11-20 | Wisconsin Alumni Research Foundation (Warf) | Vaccines comprising mutant attenuated influenza viruses |
| KR20130115105A (ko) | 2010-06-02 | 2013-10-21 | 박스터 헬쓰케어 에스에이 | Rna 바이러스를 생산하는 방법 및 헬퍼 바이러스 |
| CN102220293B (zh) * | 2011-05-26 | 2012-12-19 | 中国农业科学院上海兽医研究所 | 犬流感重组病毒及其制备方法和应用 |
| EP2747778B1 (en) | 2011-08-26 | 2017-12-06 | Wisconsin Alumni Research Foundation | Influenza viruses with mutant pb2 gene segment as live attenuated vaccines |
| EP2751260A2 (en) | 2011-09-02 | 2014-07-09 | Westfälische Wilhelms-Universität Münster | Live attenuated influenza virus |
| CA2852857A1 (en) | 2011-10-20 | 2013-04-25 | Novartis Ag | Adjuvanted influenza b virus vaccines for pediatric priming |
| GB201218195D0 (en) | 2012-10-10 | 2012-11-21 | Istituto Zooprofilattico Sperimentale Delle Venezie | Composition |
| JP2016524915A (ja) | 2013-07-15 | 2016-08-22 | ウィスコンシン アルムニ リサーチ ファンデイション | Ｍｄｃｋ、ベロ細胞又は卵内で増強された複製を有する高力価の組換えインフルエンザウイルス |
| WO2015196150A2 (en) | 2014-06-20 | 2015-12-23 | Wisconsin Alumni Research Foundation (Warf) | Mutations that confer genetic stability to additional genes in influenza viruses |
| CN104450695B (zh) * | 2014-11-26 | 2017-08-25 | 扬州大学 | A型流感病毒基因pcr扩增引物及其快速克隆方法 |
| US10633422B2 (en) | 2015-06-01 | 2020-04-28 | Wisconsin Alumni Research Foundation (Warf) | Influenza virus replication by inhibiting microRNA lec7C binding to influenza viral cRNA and mRNA |
| AU2016274655B2 (en) * | 2015-06-10 | 2021-06-17 | Hookipa Biotech Gmbh | HPV vaccines |
| EP3313439A2 (en) | 2015-06-26 | 2018-05-02 | Seqirus UK Limited | Antigenically matched influenza vaccines |
| US9890363B2 (en) | 2015-07-06 | 2018-02-13 | Wisconsin Alumni Research Foundation (Warf) | Influenza virus replication for vaccine development |
| WO2017005880A1 (en) | 2015-07-07 | 2017-01-12 | Seqirus UK Limited | Influenza potency assays |
| JP2018530314A (ja) | 2015-08-28 | 2018-10-18 | ウィスコンシン アルムニ リサーチ ファンデイション | ウイルス様粒子からの感染性インフルエンザウイルスの生成 |
| EP3417056A1 (en) | 2016-02-19 | 2018-12-26 | Wisconsin Alumni Research Foundation (WARF) | Improved influenza b virus replication for vaccine development |
| WO2019046211A1 (en) * | 2017-08-28 | 2019-03-07 | Wisconsin Alumni Research Foundation | NANOCAPSULES FOR THE ADMINISTRATION OF RIBONUCLEOPROTEINS |
| JP2021500891A (ja) | 2017-10-25 | 2021-01-14 | ウィスコンシン アルムニ リサーチ ファンデイション | 卵における複製のための安定化されたｈａを有する組換えインフルエンザウイルス |
| CN107964035B (zh) * | 2017-12-06 | 2020-07-24 | 中国科学院微生物研究所 | 一种用于流感病毒的有限复制型黏膜免疫疫苗 |
| US12343390B2 (en) | 2018-08-07 | 2025-07-01 | Wisconsin Alumni Research Foundation (Warf) | Recombinant biologically contained filovirus vaccine |
| US11389523B2 (en) | 2018-08-20 | 2022-07-19 | Wisconsin Alumni Research Foundation (Warf) | Vectors for eliciting immune responses to non-dominant epitopes in the hemagglutinin (HA) protein |
| CN109097341B (zh) * | 2018-08-28 | 2021-09-24 | 青岛农业大学 | 一种同时表达ha和hef的复制缺陷型重组流感病毒 |
| JP2022527235A (ja) | 2019-01-23 | 2022-06-01 | 義裕 河岡 | インフルエンザウイルスにおける付加的遺伝子に遺伝的安定性を付与する突然変異 |
| EP3921413A1 (en) | 2019-02-08 | 2021-12-15 | Wisconsin Alumni Research Foundation (WARF) | Humanized cell line |
| CN114929269A (zh) | 2019-05-01 | 2022-08-19 | 威斯康星校友研究基金会(Warf) | 用于疫苗开发的改进的流感病毒复制 |
| WO2021041624A2 (en) | 2019-08-27 | 2021-03-04 | Yoshihiro Kawaoka | Recombinant influenza viruses with stabilized ha for replication in eggs |
| US11739303B2 (en) | 2020-01-24 | 2023-08-29 | Wisconsin Alumni Research Foundation (Warf) | Recombinant influenza viruses with stabilized NA |
| WO2021195410A1 (en) | 2020-03-25 | 2021-09-30 | Wisconsin Alumni Research Foundation (Warf) | Recombinant multivalent influenza viruses |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5840520A (en) * | 1989-08-28 | 1998-11-24 | Aviron | Recombinant negative strand RNA virus expression systems |
| US5658772A (en) | 1989-12-22 | 1997-08-19 | E. I. Du Pont De Nemours And Company | Site-specific recombination of DNA in plant cells |
| US5919676A (en) * | 1993-06-24 | 1999-07-06 | Advec, Inc. | Adenoviral vector system comprising Cre-loxP recombination |
| EP0704533A1 (en) * | 1994-09-30 | 1996-04-03 | Bayer Ag | An attenuated vaccination virus, a method to make the virus and a pharmaceutical compositions comprising the virus |
| AU3760495A (en) * | 1994-09-30 | 1996-04-26 | Aviron | Chimeric Influenza Virus And Electroporation Method |
| CN1200675A (zh) * | 1995-10-20 | 1998-12-02 | 耶路撒冷希伯来语大学依苏姆研究开发公司 | 流感疫苗 |
| US5994526A (en) * | 1996-06-21 | 1999-11-30 | Plant Genetic Systems | Gene expression in plants |
| EP1035209A1 (en) * | 1999-03-06 | 2000-09-13 | ARTEMIS Pharmaceuticals GmbH | Stable recombinant influenza viruses free of helper viruses |
| IL145702A0 (en) * | 1999-04-06 | 2002-07-25 | Wisconsin Alumni Res Found | Recombination influenza viruses for vaccines and gene theraphy |
-
2000
- 2000-04-05 IL IL14570200A patent/IL145702A0/xx unknown
- 2000-04-05 DE DE122008000061C patent/DE122008000061I1/de active Pending
- 2000-04-05 ES ES07009953T patent/ES2373405T3/es not_active Expired - Lifetime
- 2000-04-05 KR KR1020017012743A patent/KR100702275B1/ko not_active Expired - Lifetime
- 2000-04-05 BR BRPI0009580A patent/BRPI0009580B8/pt not_active IP Right Cessation
- 2000-04-05 DK DK07009953.6T patent/DK1820853T3/da active
- 2000-04-05 DK DK10011896.7T patent/DK2345716T3/en active
- 2000-04-05 EP EP07009953A patent/EP1820853B1/en not_active Expired - Lifetime
- 2000-04-05 CA CA2365526A patent/CA2365526C/en not_active Expired - Lifetime
- 2000-04-05 MX MXPA01010082A patent/MXPA01010082A/es active IP Right Grant
- 2000-04-05 DK DK00920151T patent/DK1185615T3/da active
- 2000-04-05 DE DE122008000058C patent/DE122008000058I1/de active Pending
- 2000-04-05 PT PT00920151T patent/PT1185615E/pt unknown
- 2000-04-05 CN CN00807495XA patent/CN1350578B/zh not_active Expired - Lifetime
- 2000-04-05 JP JP2000609542A patent/JP5543048B2/ja not_active Expired - Lifetime
- 2000-04-05 DE DE60035778T patent/DE60035778T2/de not_active Expired - Lifetime
- 2000-04-05 DE DE122008000035C patent/DE122008000035I1/de active Pending
- 2000-04-05 AT AT00920151T patent/ATE368729T1/de active
- 2000-04-05 ES ES10011896.7T patent/ES2533622T3/es not_active Expired - Lifetime
- 2000-04-05 EP EP14200291.4A patent/EP2910629B1/en not_active Expired - Lifetime
- 2000-04-05 PT PT07009953T patent/PT1820853E/pt unknown
- 2000-04-05 CA CA2928263A patent/CA2928263A1/en not_active Abandoned
- 2000-04-05 EP EP00920151A patent/EP1185615B1/en not_active Expired - Lifetime
- 2000-04-05 CN CN201010155623.8A patent/CN101851636B/zh not_active Expired - Lifetime
- 2000-04-05 DE DE200812000014 patent/DE122008000014I1/de active Pending
- 2000-04-05 AU AU40733/00A patent/AU4073300A/en not_active Abandoned
- 2000-04-05 ES ES00920151T patent/ES2290024T3/es not_active Expired - Lifetime
- 2000-04-05 EP EP10011896.7A patent/EP2345716B1/en not_active Expired - Lifetime
- 2000-04-05 WO PCT/US2000/009021 patent/WO2000060050A2/en not_active Ceased
- 2000-04-05 AT AT07009953T patent/ATE525461T1/de active
-
2001
- 2001-09-30 IL IL145702A patent/IL145702A/en not_active IP Right Cessation
-
2007
- 2007-10-08 CY CY20071101295T patent/CY1108520T1/el unknown
-
2008
- 2008-11-13 NL NL300364C patent/NL300364I2/nl unknown
- 2008-11-13 NL NL300365C patent/NL300365I2/nl unknown
-
2011
- 2011-04-21 JP JP2011095240A patent/JP5775351B2/ja not_active Expired - Lifetime
- 2011-07-26 DE DE201112100037 patent/DE122011100037I1/de active Pending
- 2011-07-26 FR FR11C0027C patent/FR11C0027I2/fr active Active
- 2011-12-12 CY CY20111101234T patent/CY1112140T1/el unknown
-
2015
- 2015-03-31 JP JP2015071406A patent/JP6224645B2/ja not_active Expired - Lifetime
-
2016
- 2016-02-25 HK HK16102164.8A patent/HK1214298A1/en unknown
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2533622T3 (es) | Virus de la gripe recombinantes para vacunas y terapia génica | |
| US9157096B2 (en) | Reassortant influenza viruses for vaccines | |
| US20090017444A1 (en) | Screening method for modulators of viral transcription or replication | |
| JP2004500830A (ja) | 変異イオンチャンネルタンパク質を含んで成るウイルス | |
| AU2008201521B2 (en) | Recombinant influenza viruses for vaccines and gene therapy | |
| HK1148778B (en) | Recombinant influenza viruses for vaccines and gene therapy |