ES2528739T3 - Métodos para eliminar la manosilfosforilación de glucanos en la producción de glucoproteínas - Google Patents

Métodos para eliminar la manosilfosforilación de glucanos en la producción de glucoproteínas Download PDF

Info

Publication number
ES2528739T3
ES2528739T3 ES04806613.8T ES04806613T ES2528739T3 ES 2528739 T3 ES2528739 T3 ES 2528739T3 ES 04806613 T ES04806613 T ES 04806613T ES 2528739 T3 ES2528739 T3 ES 2528739T3
Authority
ES
Spain
Prior art keywords
nucleic acid
seq
acid sequence
glucans
pastoris
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES04806613.8T
Other languages
English (en)
Spanish (es)
Inventor
Piotr Bobrowicz
Terrance Stadheim
Stefan Wildt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GlycoFi Inc
Original Assignee
GlycoFi Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GlycoFi Inc filed Critical GlycoFi Inc
Application granted granted Critical
Publication of ES2528739T3 publication Critical patent/ES2528739T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
ES04806613.8T 2003-12-24 2004-12-22 Métodos para eliminar la manosilfosforilación de glucanos en la producción de glucoproteínas Expired - Lifetime ES2528739T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US53246103P 2003-12-24 2003-12-24
US532461P 2003-12-24
PCT/IB2004/052887 WO2005065019A2 (en) 2003-12-24 2004-12-22 Methods for eliminating mannosylphosphorylation of glycans in the production of glycoproteins

Publications (1)

Publication Number Publication Date
ES2528739T3 true ES2528739T3 (es) 2015-02-12

Family

ID=34748799

Family Applications (1)

Application Number Title Priority Date Filing Date
ES04806613.8T Expired - Lifetime ES2528739T3 (es) 2003-12-24 2004-12-22 Métodos para eliminar la manosilfosforilación de glucanos en la producción de glucoproteínas

Country Status (7)

Country Link
US (1) US7259007B2 (cg-RX-API-DMAC7.html)
EP (2) EP1696864B1 (cg-RX-API-DMAC7.html)
JP (1) JP4861830B2 (cg-RX-API-DMAC7.html)
AU (2) AU2004311545B2 (cg-RX-API-DMAC7.html)
CA (1) CA2551484C (cg-RX-API-DMAC7.html)
ES (1) ES2528739T3 (cg-RX-API-DMAC7.html)
WO (1) WO2005065019A2 (cg-RX-API-DMAC7.html)

Families Citing this family (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060024304A1 (en) * 2000-06-28 2006-02-02 Gerngross Tillman U Immunoglobulins comprising predominantly a Man5GlcNAc2 glycoform
US7795002B2 (en) * 2000-06-28 2010-09-14 Glycofi, Inc. Production of galactosylated glycoproteins in lower eukaryotes
US7598055B2 (en) * 2000-06-28 2009-10-06 Glycofi, Inc. N-acetylglucosaminyltransferase III expression in lower eukaryotes
US20060034830A1 (en) * 2000-06-28 2006-02-16 Gerngross Tillman U Immunoglobulins comprising predominantly a GalGlcNAcMan5GLcNAc2 glycoform
US7449308B2 (en) 2000-06-28 2008-11-11 Glycofi, Inc. Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
US7863020B2 (en) 2000-06-28 2011-01-04 Glycofi, Inc. Production of sialylated N-glycans in lower eukaryotes
US7029872B2 (en) * 2000-06-28 2006-04-18 Glycofi, Inc Methods for producing modified glycoproteins
US20060029604A1 (en) * 2000-06-28 2006-02-09 Gerngross Tillman U Immunoglobulins comprising predominantly a GlcNAc2Man3GlcNAc2 glycoform
US7625756B2 (en) 2000-06-28 2009-12-01 GycoFi, Inc. Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells
US8697394B2 (en) * 2000-06-28 2014-04-15 Glycofi, Inc. Production of modified glycoproteins having multiple antennary structures
US20060034828A1 (en) * 2000-06-28 2006-02-16 Gerngross Tillman U Immunoglobulins comprising predominantly a GlcNAcMAN5GLCNAC2 glycoform
CA2471551C (en) * 2001-12-27 2014-09-30 Glycofi, Inc. Methods to engineer mammalian-type carbohydrate structures
US20060024292A1 (en) * 2001-12-27 2006-02-02 Gerngross Tillman U Immunoglobulins comprising predominantly a Gal2GlcNAc2Man3GlcNAc2 glycoform
US20060034829A1 (en) * 2001-12-27 2006-02-16 Gerngross Tillman U Immunoglobulins comprising predominantly a MAN3GLCNAC2 glycoform
US6964784B2 (en) * 2002-03-07 2005-11-15 Optigenex, Inc. Method of preparation and composition of a water soluble extract of the bioactive component of the plant species uncaria for enhancing immune, anti-inflammatory, anti-tumor and dna repair processes of warm blooded animals
US7332299B2 (en) 2003-02-20 2008-02-19 Glycofi, Inc. Endomannosidases in the modification of glycoproteins in eukaryotes
CA2558635A1 (en) * 2004-03-17 2005-09-29 Glycofi, Inc. Method of engineering a cytidine monophosphate-sialic acid synthetic pathway in fungi and yeast
JP4954866B2 (ja) 2004-04-29 2012-06-20 グライコフィ， インコーポレイテッド 糖タンパク質の作製においてアルファ−マンノシダーゼ抵抗性グリカンを減少させるか又は排除する方法
EP1942935A4 (en) * 2005-09-02 2009-12-23 Glycofi Inc IMMUNOGLOBULINS CONTAINING PRESENTLY A GLCNACMAN3GLCNAC2 GLYCOFORM
AU2005336263A1 (en) * 2005-09-09 2007-03-15 Glycofi, Inc. Immunoglobulin comprising predominantly a man7GlcNAc2, Man8GlcNAc2 glycoform
US8202523B2 (en) * 2005-09-22 2012-06-19 ProSci, Inc. Glycosylated polypeptides produced in yeast mutants and methods of use thereof
EP1954815B1 (en) * 2005-11-15 2015-02-25 GlycoFi, Inc. Production of glycoproteins with reduced o-glycosylation
CA2651456A1 (en) * 2006-05-19 2007-11-29 Glycofi, Inc. Recombinant vectors
KR20090130029A (ko) * 2007-03-07 2009-12-17 글리코파이, 인크. 푸코실화가 변형된 당단백질의 제조
JP5967861B2 (ja) 2007-04-03 2016-08-10 オキシレイン ユーケー リミテッド 分子のグリコシル化
WO2008136564A1 (en) * 2007-05-04 2008-11-13 Korea Research Institute Of Bioscience And Biotechnology A novel ylmpo1 gene derived from yarrowia lipolytica and a process for preparing a glycoprotein not being mannosylphosphorylated by using a mutated yarrowia lipolytica in which ylmpo1 gene is disrupted
KR100915670B1 (ko) * 2007-05-04 2009-09-04 한국생명공학연구원 야로위아 리폴리티카 유래의 신규 ＹｌＭＰＯ１ 유전자 및이의 파쇄 균주를 이용한 만노스 인산이 제거된 당단백질생산 방법
US8637435B2 (en) * 2007-11-16 2014-01-28 Merck Sharp & Dohme Corp. Eukaryotic cell display systems
JP2011514157A (ja) 2008-02-20 2011-05-06 グライコフィ， インコーポレイテッド 蛋白質生産用ベクター及び酵母株
CA2715212A1 (en) 2008-03-03 2009-09-11 Glycofi, Inc. Surface display of recombinant proteins in lower eukaryotes
US8067339B2 (en) * 2008-07-09 2011-11-29 Merck Sharp & Dohme Corp. Surface display of whole antibodies in eukaryotes
WO2010019487A1 (en) 2008-08-12 2010-02-18 Glycofi, Inc. Improved vectors and yeast strains for protein production: ca2+ atpase overexpression
EP2401378B1 (en) 2009-02-25 2013-08-14 Merck Sharp & Dohme Corp. Metabolic engineering of a galactose assimilation pathway in the glycoengineered yeast pichia pastoris
KR102000383B1 (ko) 2009-09-29 2019-07-15 유니버시테이트 젠트 만노스-1-포스포-6-만노스 결합의 포스포-6-만노스로의 가수분해
JP2013507921A (ja) 2009-10-16 2013-03-07 メルク・シャープ・エンド・ドーム・コーポレイション 宿主細胞抗原に対する抗体に対する検出可能な交差結合活性を欠くタンパク質の、ピチア・パストリスにおける製造方法
JP2013509180A (ja) 2009-10-30 2013-03-14 メルク・シャープ・エンド・ドーム・コーポレイション 改善された分泌効率を有する組換えタンパク質の産生方法
CN102725396A (zh) 2009-10-30 2012-10-10 默沙东公司 在缺乏二肽基氨肽酶活性的巴斯德毕赤酵母中生产治疗性蛋白的方法
US20130053550A1 (en) 2009-11-19 2013-02-28 Oxyrane Uk Limited Yeast strains producing mammalian-like complex n-glycans
BR112012020882A2 (pt) 2010-02-24 2015-11-03 Merck Sharp & Dohme métodos para produzir uma glicoproteína heteróloga e para produzir uma composição de glicoproteína, célula hospedeira, composição de glicoproteína, e, uso da pichia pastoris recombinante.
AU2011258425B2 (en) 2010-05-27 2015-12-10 Merck Sharp & Dohme Llc Method for preparing antibodies having improved properties
BR112013007616B1 (pt) 2010-09-29 2023-01-24 Oxyrane Uk Limited Métodos para retirar o cap ("uncapping") de porções de manose-1- fosfo-6-manose e desmanosilar n-glicanos fosforilados em uma glicoproteína, para direcionar uma glicoproteína para o interior de uma célula de mamífero, e para converter uma glicoproteína de uma primeira forma que não se liga substancialmente a um receptor de manose-6-fosfato em uma segunda forma que se liga substancialmente a um receptor de manose-6-fosfato em uma célula de mamífero
JP6131190B2 (ja) 2010-09-29 2017-05-17 オキシレイン ユーケー リミテッド リン酸化ｎ−グリカンの脱マンノシル化
US9365846B2 (en) 2010-12-01 2016-06-14 Merck Sharp & Dohme Corp. Surface, anchored Fc-bait antibody display system
WO2012115903A2 (en) * 2011-02-25 2012-08-30 Merck Sharp & Dohme Corp. Yeast strain for the production of proteins with modified o-glycosylation
AU2012258907A1 (en) 2011-05-25 2013-11-07 Merck Sharp & Dohme Corp. Method for preparing Fc-containing polypeptides having improved properties
WO2013062940A1 (en) 2011-10-28 2013-05-02 Merck Sharp & Dohme Corp. Engineered lower eukaryotic host strains for recombinant protein expression
CA2867237C (en) 2012-03-15 2024-01-02 Wouter Vervecken Methods and materials for treatment of pompe's disease
CN104271816A (zh) 2012-05-11 2015-01-07 默沙东公司 表面锚定的轻链诱饵抗体展示系统
DK2852610T3 (en) 2012-05-23 2018-09-03 Glykos Finland Oy PRODUCTION OF FUCOSYLED GLYCOPROTEIN
AU2013335065B2 (en) 2012-10-22 2018-10-25 Merck Sharp & Dohme Llc CRZ1 mutant fungal cells
EP2925345B1 (en) * 2012-12-03 2018-09-05 Merck Sharp & Dohme Corp. Method for making o-glycosylated carboxy terminal portion (ctp) peptide-based insulin and insulin analogues
AU2015293949B2 (en) 2014-07-21 2019-07-25 Teknologian Tutkimuskeskus Vtt Oy Production of glycoproteins with mammalian-like N-glycans in filamentous fungi

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US605050A (en) * 1898-05-31 Machine for making metal spokes
US532461A (en) 1895-01-15 Surgical splint
CH586901A5 (cg-RX-API-DMAC7.html) * 1975-09-22 1977-04-15 Contraves Ag
DE2707231C3 (de) * 1977-02-19 1981-09-24 Hellma GmbH u.Co KG Glastechnische-optische Werkstätten, 7840 Müllheim Halter für Küvetten
DE2832091A1 (de) * 1978-07-21 1980-01-31 Eidenschink Henning Optisches verfahren zur bestimmung der teilchengroesse kolloidaler loesungen und messgeraet zur durchfuehrung des verfahrens
US4278437A (en) * 1979-04-09 1981-07-14 Jan Haggar Fluid specimen holder for biological fluid testing
USD271335S (en) * 1981-03-16 1983-11-08 Simons Sanford L Cuvette holder
JPH01291830A (ja) * 1988-05-20 1989-11-24 Terumo Corp 採血管ホルダー
JP3199850B2 (ja) * 1992-08-04 2001-08-20 興和株式会社 血小板凝集能測定装置
US5530540A (en) * 1994-08-03 1996-06-25 Wyatt Technology Corporation Light scattering measurement cell for very small volumes
US5900132A (en) * 1995-03-23 1999-05-04 Beckman Coulter, Inc. Capillary holder
SE504193C2 (sv) * 1995-04-21 1996-12-02 Hemocue Ab Kapillär mikrokyvett
US5733507A (en) * 1995-06-07 1998-03-31 Inphocyte, Inc. Biological cell sample holder for use in infrared and/or Raman spectroscopy analysis holder
HUP0003903A1 (en) * 1995-11-16 2000-10-28 Process for settling down particles floating in liquid and for observing them optically
DE69610218T2 (de) * 1995-11-17 2001-01-25 Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno, Den Haag/S'gravenhage Photometrisches messsystem und halter für ein solches system
US5817519A (en) * 1995-12-28 1998-10-06 Bayer Corporation Automated method and device for identifying and quantifying platelets and for determining platelet activation state using whole blood samples
DE29607239U1 (de) * 1996-04-23 1996-06-05 J & M Analytische Mess- und Regeltechnik GmbH, 73431 Aalen Kapillarhalter
US6618144B1 (en) * 1999-10-15 2003-09-09 The Administrators Of The Tulane Educational Fund Device and method of simultaneously measuring the light scattering from multiple liquid samples containing polymers and/or colloids
US6043871A (en) * 1997-03-03 2000-03-28 Brigham Young University System and method for measuring blood platelet function
US6004818A (en) * 1997-08-07 1999-12-21 Chrono-Log Corporation Aggregometer with disposable test cell
AU3649199A (en) * 1998-04-17 1999-11-08 Ljl Biosystems, Inc. Sample-holding devices and systems
DE69910006T2 (de) * 1998-05-13 2004-05-27 Bayer Corp. Durchflussküvette zur spektroskopischen untersuchung einer probe
US6016193A (en) * 1998-06-23 2000-01-18 Awareness Technology, Inc. Cuvette holder for coagulation assay test
GB2339019B (en) * 1998-06-30 2000-06-14 Khaled Karrai Sample holder apparatus
EP0984062A1 (en) * 1998-09-04 2000-03-08 Cytos Biotechnology AG Production of human erythropoietin
US6228652B1 (en) * 1999-02-16 2001-05-08 Coulter International Corp. Method and apparatus for analyzing cells in a whole blood sample
USD442287S1 (en) * 1999-11-11 2001-05-15 Hemocue Ab Cuvette holder
US6375027B1 (en) * 1999-11-18 2002-04-23 Harvey M. Thomas Holder for blood sample tubes
WO2001058493A1 (en) * 2000-02-11 2001-08-16 Maxygen Aps Conjugates of follicle stimulating hormones
WO2001088143A1 (en) 2000-05-17 2001-11-22 Mitsubishi Pharma Corporation Process for producing protein with reduction of acidic sugar chain and glycoprotein produced thereby
JP2001349825A (ja) * 2000-06-06 2001-12-21 Kowa Co キュベット・スタンド及びキュベット付きスタンド
US7029872B2 (en) * 2000-06-28 2006-04-18 Glycofi, Inc Methods for producing modified glycoproteins
US6798520B2 (en) * 2002-03-22 2004-09-28 Diversa Corporation Method for intensifying the optical detection of samples that are held in solution in the through-hole wells of a holding tray
US6992759B2 (en) * 2002-10-21 2006-01-31 Nippon Shokubai Co., Ltd. Sample holder for spectrum measurement and spectrophotometer
US6867857B2 (en) * 2002-10-29 2005-03-15 Nanostream, Inc. Flow cell for optical analysis of a fluid
US6774994B1 (en) * 2003-08-13 2004-08-10 Wyatt Technology Corporation Method and apparatus for determining absolute number densities of particles in suspension
US7671974B2 (en) * 2003-10-29 2010-03-02 Chf Solutions Inc. Cuvette apparatus and system for measuring optical properties of a liquid such as blood
WO2008136564A1 (en) * 2007-05-04 2008-11-13 Korea Research Institute Of Bioscience And Biotechnology A novel ylmpo1 gene derived from yarrowia lipolytica and a process for preparing a glycoprotein not being mannosylphosphorylated by using a mutated yarrowia lipolytica in which ylmpo1 gene is disrupted

Also Published As

Publication number Publication date
EP1696864A4 (en) 2008-03-26
CA2551484C (en) 2015-03-31
WO2005065019A2 (en) 2005-07-21
JP2007530014A (ja) 2007-11-01
AU2004311545B2 (en) 2008-11-06
CA2551484A1 (en) 2005-07-21
WO2005065019A3 (en) 2007-01-25
EP1696864B1 (en) 2014-11-12
AU2004311545A1 (en) 2005-07-21
US7259007B2 (en) 2007-08-21
EP1696864A2 (en) 2006-09-06
EP2341128A1 (en) 2011-07-06
AU2008249203B2 (en) 2012-08-16
JP4861830B2 (ja) 2012-01-25
US20060160179A1 (en) 2006-07-20
AU2008249203A1 (en) 2008-12-18

Similar Documents

Publication Publication Date Title
EP1696864B1 (en) Methods for eliminating mannosylphosphorylation of glycans in the production of glycoproteins
JP4954866B2 (ja) 糖タンパク質の作製においてアルファ−マンノシダーゼ抵抗性グリカンを減少させるか又は排除する方法
JP2007534330A5 (cg-RX-API-DMAC7.html)
US8062879B2 (en) URA5 gene and methods for stable genetic integration in yeast
KR20140015137A (ko) 분비 효율이 개선된 재조합 단백질의 생산 방법
JP2011514157A (ja) 蛋白質生産用ベクター及び酵母株
JP2012506710A (ja) 宿主細胞中での糖鎖付加タンパク質の産生のための新規ツール
MX2012004994A (es) Metodo para producir proteinas terapeuticas en pichia pastoris que carecen de actividad de dipeptidil aminopeptidasa.
US7479389B2 (en) ARG1, ARG2, ARG3, HIS1, HIS2, HIS5, HIS6 genes and methods for stable genetic integration
KR20140114278A (ko) 변형된 ｏ-글리코실화를 갖는 단백질의 생산을 위한 효모 균주
KR20140134272A (ko) 효모에서 만노실트랜스퍼라제 활성을 감소시키는 방법
US20120100619A1 (en) Pichia pastoris loci encoding enzymes in the methionine biosynthetic pathway
JP5752582B2 (ja) 糖タンパク質の作製においてアルファ−マンノシダーゼ抵抗性グリカンを減少させるか又は排除する方法
CA2876864A1 (en) Methods for eliminating mannosylphosphorylation of glycans in the production of glycoproteins
KR101419266B1 (ko) 한세눌라 폴리모르파 유래의 단백질 ｏ-만노실 전이효소의 신규 유전자 및 이 유전자가 결손된 한세눌라 폴리모르파 변이주
WO2012064619A1 (en) Pichia pastoris loci encoding enzymes in the proline biosynthetic pathway
US20120100617A1 (en) Pichia pastoris loci encoding enzymes in the adenine biosynthetic pathway
US20120100622A1 (en) Pichia pastoris loci encoding enzymes in the uracil biosynthetic pathway
US20120100618A1 (en) Pichia pastoris loci encoding enzymes in the histidine biosynthetic pathway