JP4861830B2 - 糖タンパク質の産生において、グリカンのマンノシルリン酸化を除去する方法 - Google Patents
糖タンパク質の産生において、グリカンのマンノシルリン酸化を除去する方法 Download PDFInfo
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- JP4861830B2 JP4861830B2 JP2006546463A JP2006546463A JP4861830B2 JP 4861830 B2 JP4861830 B2 JP 4861830B2 JP 2006546463 A JP2006546463 A JP 2006546463A JP 2006546463 A JP2006546463 A JP 2006546463A JP 4861830 B2 JP4861830 B2 JP 4861830B2
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/005—Glycopeptides, glycoproteins
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- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US53246103P | 2003-12-24 | 2003-12-24 | |
| US60/532,461 | 2003-12-24 | ||
| PCT/IB2004/052887 WO2005065019A2 (en) | 2003-12-24 | 2004-12-22 | Methods for eliminating mannosylphosphorylation of glycans in the production of glycoproteins |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2007530014A JP2007530014A (ja) | 2007-11-01 |
| JP2007530014A5 JP2007530014A5 (cg-RX-API-DMAC7.html) | 2008-02-07 |
| JP4861830B2 true JP4861830B2 (ja) | 2012-01-25 |
Family
ID=34748799
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2006546463A Expired - Lifetime JP4861830B2 (ja) | 2003-12-24 | 2004-12-22 | 糖タンパク質の産生において、グリカンのマンノシルリン酸化を除去する方法 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US7259007B2 (cg-RX-API-DMAC7.html) |
| EP (2) | EP1696864B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP4861830B2 (cg-RX-API-DMAC7.html) |
| AU (2) | AU2004311545B2 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2551484C (cg-RX-API-DMAC7.html) |
| ES (1) | ES2528739T3 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2005065019A2 (cg-RX-API-DMAC7.html) |
Families Citing this family (52)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US20060024304A1 (en) * | 2000-06-28 | 2006-02-02 | Gerngross Tillman U | Immunoglobulins comprising predominantly a Man5GlcNAc2 glycoform |
| US7795002B2 (en) * | 2000-06-28 | 2010-09-14 | Glycofi, Inc. | Production of galactosylated glycoproteins in lower eukaryotes |
| US7598055B2 (en) * | 2000-06-28 | 2009-10-06 | Glycofi, Inc. | N-acetylglucosaminyltransferase III expression in lower eukaryotes |
| US20060034830A1 (en) * | 2000-06-28 | 2006-02-16 | Gerngross Tillman U | Immunoglobulins comprising predominantly a GalGlcNAcMan5GLcNAc2 glycoform |
| US7449308B2 (en) | 2000-06-28 | 2008-11-11 | Glycofi, Inc. | Combinatorial DNA library for producing modified N-glycans in lower eukaryotes |
| US7863020B2 (en) | 2000-06-28 | 2011-01-04 | Glycofi, Inc. | Production of sialylated N-glycans in lower eukaryotes |
| US7029872B2 (en) * | 2000-06-28 | 2006-04-18 | Glycofi, Inc | Methods for producing modified glycoproteins |
| US20060029604A1 (en) * | 2000-06-28 | 2006-02-09 | Gerngross Tillman U | Immunoglobulins comprising predominantly a GlcNAc2Man3GlcNAc2 glycoform |
| US7625756B2 (en) | 2000-06-28 | 2009-12-01 | GycoFi, Inc. | Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells |
| US8697394B2 (en) * | 2000-06-28 | 2014-04-15 | Glycofi, Inc. | Production of modified glycoproteins having multiple antennary structures |
| US20060034828A1 (en) * | 2000-06-28 | 2006-02-16 | Gerngross Tillman U | Immunoglobulins comprising predominantly a GlcNAcMAN5GLCNAC2 glycoform |
| CA2471551C (en) * | 2001-12-27 | 2014-09-30 | Glycofi, Inc. | Methods to engineer mammalian-type carbohydrate structures |
| US20060024292A1 (en) * | 2001-12-27 | 2006-02-02 | Gerngross Tillman U | Immunoglobulins comprising predominantly a Gal2GlcNAc2Man3GlcNAc2 glycoform |
| US20060034829A1 (en) * | 2001-12-27 | 2006-02-16 | Gerngross Tillman U | Immunoglobulins comprising predominantly a MAN3GLCNAC2 glycoform |
| US6964784B2 (en) * | 2002-03-07 | 2005-11-15 | Optigenex, Inc. | Method of preparation and composition of a water soluble extract of the bioactive component of the plant species uncaria for enhancing immune, anti-inflammatory, anti-tumor and dna repair processes of warm blooded animals |
| US7332299B2 (en) | 2003-02-20 | 2008-02-19 | Glycofi, Inc. | Endomannosidases in the modification of glycoproteins in eukaryotes |
| CA2558635A1 (en) * | 2004-03-17 | 2005-09-29 | Glycofi, Inc. | Method of engineering a cytidine monophosphate-sialic acid synthetic pathway in fungi and yeast |
| JP4954866B2 (ja) | 2004-04-29 | 2012-06-20 | グライコフィ， インコーポレイテッド | 糖タンパク質の作製においてアルファ−マンノシダーゼ抵抗性グリカンを減少させるか又は排除する方法 |
| EP1942935A4 (en) * | 2005-09-02 | 2009-12-23 | Glycofi Inc | IMMUNOGLOBULINS CONTAINING PRESENTLY A GLCNACMAN3GLCNAC2 GLYCOFORM |
| AU2005336263A1 (en) * | 2005-09-09 | 2007-03-15 | Glycofi, Inc. | Immunoglobulin comprising predominantly a man7GlcNAc2, Man8GlcNAc2 glycoform |
| US8202523B2 (en) * | 2005-09-22 | 2012-06-19 | ProSci, Inc. | Glycosylated polypeptides produced in yeast mutants and methods of use thereof |
| EP1954815B1 (en) * | 2005-11-15 | 2015-02-25 | GlycoFi, Inc. | Production of glycoproteins with reduced o-glycosylation |
| CA2651456A1 (en) * | 2006-05-19 | 2007-11-29 | Glycofi, Inc. | Recombinant vectors |
| KR20090130029A (ko) * | 2007-03-07 | 2009-12-17 | 글리코파이, 인크. | 푸코실화가 변형된 당단백질의 제조 |
| JP5967861B2 (ja) | 2007-04-03 | 2016-08-10 | オキシレイン ユーケー リミテッド | 分子のグリコシル化 |
| WO2008136564A1 (en) * | 2007-05-04 | 2008-11-13 | Korea Research Institute Of Bioscience And Biotechnology | A novel ylmpo1 gene derived from yarrowia lipolytica and a process for preparing a glycoprotein not being mannosylphosphorylated by using a mutated yarrowia lipolytica in which ylmpo1 gene is disrupted |
| KR100915670B1 (ko) * | 2007-05-04 | 2009-09-04 | 한국생명공학연구원 | 야로위아 리폴리티카 유래의 신규 ＹｌＭＰＯ１ 유전자 및이의 파쇄 균주를 이용한 만노스 인산이 제거된 당단백질생산 방법 |
| US8637435B2 (en) * | 2007-11-16 | 2014-01-28 | Merck Sharp & Dohme Corp. | Eukaryotic cell display systems |
| JP2011514157A (ja) | 2008-02-20 | 2011-05-06 | グライコフィ， インコーポレイテッド | 蛋白質生産用ベクター及び酵母株 |
| CA2715212A1 (en) | 2008-03-03 | 2009-09-11 | Glycofi, Inc. | Surface display of recombinant proteins in lower eukaryotes |
| US8067339B2 (en) * | 2008-07-09 | 2011-11-29 | Merck Sharp & Dohme Corp. | Surface display of whole antibodies in eukaryotes |
| WO2010019487A1 (en) | 2008-08-12 | 2010-02-18 | Glycofi, Inc. | Improved vectors and yeast strains for protein production: ca2+ atpase overexpression |
| EP2401378B1 (en) | 2009-02-25 | 2013-08-14 | Merck Sharp & Dohme Corp. | Metabolic engineering of a galactose assimilation pathway in the glycoengineered yeast pichia pastoris |
| KR102000383B1 (ko) | 2009-09-29 | 2019-07-15 | 유니버시테이트 젠트 | 만노스-1-포스포-6-만노스 결합의 포스포-6-만노스로의 가수분해 |
| JP2013507921A (ja) | 2009-10-16 | 2013-03-07 | メルク・シャープ・エンド・ドーム・コーポレイション | 宿主細胞抗原に対する抗体に対する検出可能な交差結合活性を欠くタンパク質の、ピチア・パストリスにおける製造方法 |
| JP2013509180A (ja) | 2009-10-30 | 2013-03-14 | メルク・シャープ・エンド・ドーム・コーポレイション | 改善された分泌効率を有する組換えタンパク質の産生方法 |
| CN102725396A (zh) | 2009-10-30 | 2012-10-10 | 默沙东公司 | 在缺乏二肽基氨肽酶活性的巴斯德毕赤酵母中生产治疗性蛋白的方法 |
| US20130053550A1 (en) | 2009-11-19 | 2013-02-28 | Oxyrane Uk Limited | Yeast strains producing mammalian-like complex n-glycans |
| BR112012020882A2 (pt) | 2010-02-24 | 2015-11-03 | Merck Sharp & Dohme | métodos para produzir uma glicoproteína heteróloga e para produzir uma composição de glicoproteína, célula hospedeira, composição de glicoproteína, e, uso da pichia pastoris recombinante. |
| AU2011258425B2 (en) | 2010-05-27 | 2015-12-10 | Merck Sharp & Dohme Llc | Method for preparing antibodies having improved properties |
| BR112013007616B1 (pt) | 2010-09-29 | 2023-01-24 | Oxyrane Uk Limited | Métodos para retirar o cap ("uncapping") de porções de manose-1- fosfo-6-manose e desmanosilar n-glicanos fosforilados em uma glicoproteína, para direcionar uma glicoproteína para o interior de uma célula de mamífero, e para converter uma glicoproteína de uma primeira forma que não se liga substancialmente a um receptor de manose-6-fosfato em uma segunda forma que se liga substancialmente a um receptor de manose-6-fosfato em uma célula de mamífero |
| JP6131190B2 (ja) | 2010-09-29 | 2017-05-17 | オキシレイン ユーケー リミテッド | リン酸化ｎ−グリカンの脱マンノシル化 |
| US9365846B2 (en) | 2010-12-01 | 2016-06-14 | Merck Sharp & Dohme Corp. | Surface, anchored Fc-bait antibody display system |
| WO2012115903A2 (en) * | 2011-02-25 | 2012-08-30 | Merck Sharp & Dohme Corp. | Yeast strain for the production of proteins with modified o-glycosylation |
| AU2012258907A1 (en) | 2011-05-25 | 2013-11-07 | Merck Sharp & Dohme Corp. | Method for preparing Fc-containing polypeptides having improved properties |
| WO2013062940A1 (en) | 2011-10-28 | 2013-05-02 | Merck Sharp & Dohme Corp. | Engineered lower eukaryotic host strains for recombinant protein expression |
| CA2867237C (en) | 2012-03-15 | 2024-01-02 | Wouter Vervecken | Methods and materials for treatment of pompe's disease |
| CN104271816A (zh) | 2012-05-11 | 2015-01-07 | 默沙东公司 | 表面锚定的轻链诱饵抗体展示系统 |
| DK2852610T3 (en) | 2012-05-23 | 2018-09-03 | Glykos Finland Oy | PRODUCTION OF FUCOSYLED GLYCOPROTEIN |
| AU2013335065B2 (en) | 2012-10-22 | 2018-10-25 | Merck Sharp & Dohme Llc | CRZ1 mutant fungal cells |
| EP2925345B1 (en) * | 2012-12-03 | 2018-09-05 | Merck Sharp & Dohme Corp. | Method for making o-glycosylated carboxy terminal portion (ctp) peptide-based insulin and insulin analogues |
| AU2015293949B2 (en) | 2014-07-21 | 2019-07-25 | Teknologian Tutkimuskeskus Vtt Oy | Production of glycoproteins with mammalian-like N-glycans in filamentous fungi |
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| WO2001088143A1 (en) * | 2000-05-17 | 2001-11-22 | Mitsubishi Pharma Corporation | Process for producing protein with reduction of acidic sugar chain and glycoprotein produced thereby |
| US20020137134A1 (en) * | 2000-06-28 | 2002-09-26 | Gerngross Tillman U. | Methods for producing modified glycoproteins |
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-
2004
- 2004-12-22 EP EP04806613.8A patent/EP1696864B1/en not_active Expired - Lifetime
- 2004-12-22 AU AU2004311545A patent/AU2004311545B2/en not_active Ceased
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- 2004-12-22 JP JP2006546463A patent/JP4861830B2/ja not_active Expired - Lifetime
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- 2004-12-22 EP EP10180809A patent/EP2341128A1/en not_active Withdrawn
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2008
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001088143A1 (en) * | 2000-05-17 | 2001-11-22 | Mitsubishi Pharma Corporation | Process for producing protein with reduction of acidic sugar chain and glycoprotein produced thereby |
| US20020137134A1 (en) * | 2000-06-28 | 2002-09-26 | Gerngross Tillman U. | Methods for producing modified glycoproteins |
Also Published As
| Publication number | Publication date |
|---|---|
| EP1696864A4 (en) | 2008-03-26 |
| ES2528739T3 (es) | 2015-02-12 |
| CA2551484C (en) | 2015-03-31 |
| WO2005065019A2 (en) | 2005-07-21 |
| JP2007530014A (ja) | 2007-11-01 |
| AU2004311545B2 (en) | 2008-11-06 |
| CA2551484A1 (en) | 2005-07-21 |
| WO2005065019A3 (en) | 2007-01-25 |
| EP1696864B1 (en) | 2014-11-12 |
| AU2004311545A1 (en) | 2005-07-21 |
| US7259007B2 (en) | 2007-08-21 |
| EP1696864A2 (en) | 2006-09-06 |
| EP2341128A1 (en) | 2011-07-06 |
| AU2008249203B2 (en) | 2012-08-16 |
| US20060160179A1 (en) | 2006-07-20 |
| AU2008249203A1 (en) | 2008-12-18 |
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