ES2421294T3 - Producción microbiana de ácido L-ascórbico - Google Patents
Producción microbiana de ácido L-ascórbico Download PDFInfo
- Publication number
- ES2421294T3 ES2421294T3 ES04738146T ES04738146T ES2421294T3 ES 2421294 T3 ES2421294 T3 ES 2421294T3 ES 04738146 T ES04738146 T ES 04738146T ES 04738146 T ES04738146 T ES 04738146T ES 2421294 T3 ES2421294 T3 ES 2421294T3
- Authority
- ES
- Spain
- Prior art keywords
- ascorbic acid
- sorbosone
- microorganism
- production
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 title claims abstract description 342
- 229960005070 ascorbic acid Drugs 0.000 title claims abstract description 117
- 239000002211 L-ascorbic acid Substances 0.000 title claims abstract description 116
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- 238000004519 manufacturing process Methods 0.000 title claims description 98
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- 239000002773 nucleotide Substances 0.000 claims abstract description 60
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 60
- 108010053002 L-sorbosone dehydrogenase Proteins 0.000 claims abstract description 57
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- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims abstract description 16
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- 238000002270 exclusion chromatography Methods 0.000 description 1
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- YAGKRVSRTSUGEY-UHFFFAOYSA-N ferricyanide Chemical compound [Fe+3].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] YAGKRVSRTSUGEY-UHFFFAOYSA-N 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
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- 238000009776 industrial production Methods 0.000 description 1
- 229910001867 inorganic solvent Inorganic materials 0.000 description 1
- 239000003049 inorganic solvent Substances 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 1
- BJHIKXHVCXFQLS-UYFOZJQFSA-N keto-D-fructose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C(=O)CO BJHIKXHVCXFQLS-UYFOZJQFSA-N 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
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- MHWLWQUZZRMNGJ-UHFFFAOYSA-N nalidixic acid Chemical compound C1=C(C)N=C2N(CC)C=C(C(O)=O)C(=O)C2=C1 MHWLWQUZZRMNGJ-UHFFFAOYSA-N 0.000 description 1
- 229960000210 nalidixic acid Drugs 0.000 description 1
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- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 description 1
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- 229920000642 polymer Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
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- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 229940017794 potassium ascorbate Drugs 0.000 description 1
- 235000019275 potassium ascorbate Nutrition 0.000 description 1
- CONVKSGEGAVTMB-RXSVEWSESA-M potassium-L-ascorbate Chemical compound [K+].OC[C@H](O)[C@H]1OC(=O)C(O)=C1[O-] CONVKSGEGAVTMB-RXSVEWSESA-M 0.000 description 1
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- JQXXHWHPUNPDRT-WLSIYKJHSA-N rifampicin Chemical compound O([C@](C1=O)(C)O/C=C/[C@@H]([C@H]([C@@H](OC(C)=O)[C@H](C)[C@H](O)[C@H](C)[C@@H](O)[C@@H](C)\C=C\C=C(C)/C(=O)NC=2C(O)=C3C([O-])=C4C)C)OC)C4=C1C3=C(O)C=2\C=N\N1CC[NH+](C)CC1 JQXXHWHPUNPDRT-WLSIYKJHSA-N 0.000 description 1
- 229960001225 rifampicin Drugs 0.000 description 1
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- PPASLZSBLFJQEF-RKJRWTFHSA-M sodium ascorbate Substances [Na+].OC[C@@H](O)[C@H]1OC(=O)C(O)=C1[O-] PPASLZSBLFJQEF-RKJRWTFHSA-M 0.000 description 1
- 235000010378 sodium ascorbate Nutrition 0.000 description 1
- 229960005055 sodium ascorbate Drugs 0.000 description 1
- 235000017550 sodium carbonate Nutrition 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- 235000010344 sodium nitrate Nutrition 0.000 description 1
- 239000004317 sodium nitrate Substances 0.000 description 1
- 239000012064 sodium phosphate buffer Substances 0.000 description 1
- PPASLZSBLFJQEF-RXSVEWSESA-M sodium-L-ascorbate Chemical compound [Na+].OC[C@H](O)[C@H]1OC(=O)C(O)=C1[O-] PPASLZSBLFJQEF-RXSVEWSESA-M 0.000 description 1
- 238000005063 solubilization Methods 0.000 description 1
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- 229960002180 tetracycline Drugs 0.000 description 1
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- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
- 229940038773 trisodium citrate Drugs 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/02—Oxygen as only ring hetero atoms
- C12P17/04—Oxygen as only ring hetero atoms containing a five-membered hetero ring, e.g. griseofulvin, vitamin C
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/58—Aldonic, ketoaldonic or saccharic acids
- C12P7/60—2-Ketogulonic acid
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP03017677 | 2003-08-14 | ||
| EP03017677 | 2003-08-14 | ||
| PCT/CH2004/000511 WO2005017159A2 (en) | 2003-08-14 | 2004-08-16 | Microbial production of l-ascorbic acid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2421294T3 true ES2421294T3 (es) | 2013-08-30 |
Family
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| ES04738146T Expired - Lifetime ES2421294T3 (es) | 2003-08-14 | 2004-08-16 | Producción microbiana de ácido L-ascórbico |
| ES10184019.7T Expired - Lifetime ES2593811T3 (es) | 2003-08-14 | 2004-08-16 | Producción microbiana de ácido L-ascórbico |
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| Application Number | Title | Priority Date | Filing Date |
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| ES10184019.7T Expired - Lifetime ES2593811T3 (es) | 2003-08-14 | 2004-08-16 | Producción microbiana de ácido L-ascórbico |
Country Status (9)
| Country | Link |
|---|---|
| US (3) | US7700723B2 (https=) |
| EP (2) | EP2348113B1 (https=) |
| JP (1) | JP4896718B2 (https=) |
| KR (1) | KR101311562B1 (https=) |
| CN (2) | CN104004776A (https=) |
| BR (1) | BRPI0413542A (https=) |
| EA (1) | EA009287B1 (https=) |
| ES (2) | ES2421294T3 (https=) |
| WO (2) | WO2005017172A1 (https=) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006084724A1 (en) * | 2005-02-11 | 2006-08-17 | Dsm Ip Assets B.V. | Novel gene rcs 28 |
| WO2006084645A1 (en) * | 2005-02-11 | 2006-08-17 | Dsm Ip Assets B.V. | Novel gene rcs 10 |
| US20080193972A1 (en) * | 2005-02-11 | 2008-08-14 | Bastian Chevreux | Gene Rcs 25 |
| WO2006084719A1 (en) * | 2005-02-11 | 2006-08-17 | Dsm Ip Assets B.V. | Fermentive vitamin c production |
| US20080176297A1 (en) * | 2005-02-11 | 2008-07-24 | Bastien Chevreux | Gene Rcs 21 |
| WO2006084737A1 (en) * | 2005-02-11 | 2006-08-17 | Dsm Ip Assets B.V. | Gene rcs 23 |
| WO2006084717A1 (en) * | 2005-02-11 | 2006-08-17 | Dsm Ip Assets B.V. | Gene sms 02 |
| WO2006084643A2 (en) * | 2005-02-11 | 2006-08-17 | Dsm Ip Assets B.V. | Novel gene vcs 07 |
| US20080305532A1 (en) * | 2005-02-11 | 2008-12-11 | Bastien Chevreux | Gene For Coenzyme Pqq Synthesis Protein B From Gluconobacter Oxydans |
| CN101316929A (zh) | 2005-09-08 | 2008-12-03 | 帝斯曼知识产权资产管理有限公司 | 新颖的基因sms 37 |
| EP1931785A2 (en) * | 2005-09-09 | 2008-06-18 | DSMIP Assets B.V. | Novel gene gms 01 |
| CN101292025A (zh) * | 2005-09-09 | 2008-10-22 | 帝斯曼知识产权资产管理有限公司 | 来自Gluconobacter oxydans的NADP依赖性葡糖脱氢酶 |
| FR2906817B1 (fr) * | 2006-10-04 | 2012-04-06 | Gervais Danone Sa | Procede de culture pour favoriser la production de vitamine k2 par les bacteries lactiques et ses applications a la preparation de produits alimentaires |
| EP1918382A1 (en) * | 2006-11-02 | 2008-05-07 | DSMIP Assets B.V. | Semi-continuous cascade fermentation process for pantothenate production |
| EP1918383A1 (en) * | 2006-11-02 | 2008-05-07 | DSMIP Assets B.V. | Semi-continuous fermentation process for pantothenate production |
| CN101918575A (zh) * | 2008-01-10 | 2010-12-15 | 帝斯曼知识产权资产管理有限公司 | 新颖的基因sms 44 |
| CN103073601B (zh) * | 2013-02-01 | 2015-08-19 | 东北制药集团股份有限公司 | 一种2-酮基-l-古龙酸溶液加晶种的可控浓缩结晶方法 |
| NZ743055A (en) * | 2013-03-08 | 2020-03-27 | Xyleco Inc | Equipment protecting enclosures |
| AU2014241191A1 (en) | 2013-03-27 | 2015-10-22 | Sample Technologies, Inc. | Recombinant phage and bacterial detection methods |
| CN105531382A (zh) * | 2013-06-19 | 2016-04-27 | 六品科技公司 | 基于噬菌体的细菌检测法 |
| CA2921883C (en) * | 2013-08-23 | 2020-03-24 | Sandoz Ag | Novel adsorbent composition and use thereof |
| WO2015148680A1 (en) * | 2014-03-25 | 2015-10-01 | Ginkgo Bioworks, Inc. | Methods and genetic systems for cell engineering |
| CN116053017B (zh) * | 2022-11-04 | 2023-08-22 | 医波(厦门)科技有限公司 | 一种复合磁性微球及其制备方法和应用 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5082785A (en) | 1987-01-30 | 1992-01-21 | Hoffmann-La Roche Inc. | Biosynthesis of 2 keto-l-gulonic acid |
| EP0373181B1 (en) | 1988-01-14 | 1995-11-08 | F. Hoffmann-La Roche Ag | Novel coenzyme independent l-sorbosone dehydrogenase |
| EP0911415B1 (en) * | 1990-09-17 | 2005-12-07 | DSM IP Assets B.V. | Microbiological manufacture of L-ascorbic acid |
| US5376544A (en) | 1992-09-08 | 1994-12-27 | Rutgers The State University Of New Jersey | Enzymes for the production of 2-keto-L-gulonic acid |
| AU6731896A (en) * | 1995-07-17 | 1997-02-18 | Fraunhofer-Gesellschaft Zur Forderung Der Angewandten Forschung E.V. | Cyclitol |
| DE69821326T2 (de) * | 1997-12-01 | 2004-11-18 | Dsm Ip Assets B.V. | Aldehyd-Dehydrogenase |
| US20020012979A1 (en) * | 1998-06-08 | 2002-01-31 | Alan Berry | Vitamin c production in microorganisms and plants |
| GB0119864D0 (en) * | 2001-08-15 | 2001-10-10 | Cerestar Holding Bv | Process for the manufacture of 2-keto-L-gulonic acid |
| US20050153412A1 (en) * | 2002-04-22 | 2005-07-14 | Tatsuo Hoshino | Aldehyde dehydrogenase II |
| CN1659275A (zh) * | 2002-06-06 | 2005-08-24 | Dsmip资产有限公司 | 醛脱氢酶 |
| KR101070110B1 (ko) * | 2002-09-27 | 2011-10-05 | 디에스엠 아이피 어셋츠 비.브이. | 알데히드 데하이드로게나제 유전자 |
| KR101021234B1 (ko) | 2002-09-27 | 2011-03-11 | 디에스엠 아이피 어셋츠 비.브이. | 미생물에 의한 비타민 ｃ의 생성 방법 |
| EP1543137A1 (en) * | 2002-09-27 | 2005-06-22 | DSM IP Assets B.V. | Process for producing vitamin c |
| CN1914326B (zh) | 2004-01-30 | 2012-04-18 | 帝斯曼知识产权资产管理有限公司 | 维生素c的微生物生产方法 |
| US20080226126A1 (en) * | 2005-01-31 | 2008-09-18 | Yoshinori Ohno | Object-Tracking Apparatus, Microscope System, and Object-Tracking Program |
| WO2006084719A1 (en) | 2005-02-11 | 2006-08-17 | Dsm Ip Assets B.V. | Fermentive vitamin c production |
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2004
- 2004-08-16 WO PCT/CH2004/000512 patent/WO2005017172A1/en not_active Ceased
- 2004-08-16 CN CN201410171433.3A patent/CN104004776A/zh active Pending
- 2004-08-16 BR BRPI0413542-3A patent/BRPI0413542A/pt not_active Application Discontinuation
- 2004-08-16 ES ES04738146T patent/ES2421294T3/es not_active Expired - Lifetime
- 2004-08-16 EA EA200600408A patent/EA009287B1/ru not_active IP Right Cessation
- 2004-08-16 JP JP2006522868A patent/JP4896718B2/ja not_active Expired - Fee Related
- 2004-08-16 EP EP10184019.7A patent/EP2348113B1/en not_active Expired - Lifetime
- 2004-08-16 ES ES10184019.7T patent/ES2593811T3/es not_active Expired - Lifetime
- 2004-08-16 CN CN200480023361.2A patent/CN1882691B/zh not_active Expired - Fee Related
- 2004-08-16 KR KR1020067003014A patent/KR101311562B1/ko not_active Expired - Fee Related
- 2004-08-16 WO PCT/CH2004/000511 patent/WO2005017159A2/en not_active Ceased
- 2004-08-16 EP EP04738146.2A patent/EP1664303B1/en not_active Expired - Lifetime
- 2004-08-16 US US10/567,763 patent/US7700723B2/en not_active Expired - Lifetime
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2010
- 2010-02-08 US US12/701,748 patent/US8338144B2/en not_active Expired - Fee Related
-
2012
- 2012-11-21 US US13/683,487 patent/US20130217080A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US8338144B2 (en) | 2012-12-25 |
| CN104004776A (zh) | 2014-08-27 |
| EA200600408A1 (ru) | 2006-08-25 |
| JP2007502102A (ja) | 2007-02-08 |
| US20070184534A1 (en) | 2007-08-09 |
| WO2005017159A2 (en) | 2005-02-24 |
| WO2005017159A3 (en) | 2005-11-03 |
| US7700723B2 (en) | 2010-04-20 |
| EP2348113A2 (en) | 2011-07-27 |
| EP2348113A3 (en) | 2012-11-14 |
| EP1664303A2 (en) | 2006-06-07 |
| CN1882691B (zh) | 2014-05-28 |
| US20130217080A1 (en) | 2013-08-22 |
| JP4896718B2 (ja) | 2012-03-14 |
| CN1882691A (zh) | 2006-12-20 |
| KR101311562B1 (ko) | 2013-09-26 |
| WO2005017172A1 (en) | 2005-02-24 |
| BRPI0413542A (pt) | 2006-10-17 |
| EA009287B1 (ru) | 2007-12-28 |
| EP1664303B1 (en) | 2013-04-24 |
| US20100203598A1 (en) | 2010-08-12 |
| ES2593811T3 (es) | 2016-12-13 |
| KR20060064629A (ko) | 2006-06-13 |
| EP2348113B1 (en) | 2016-06-29 |
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