ES2414286T3 - Detección de ácidos nucleicos - Google Patents

Detección de ácidos nucleicos Download PDF

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Publication number
ES2414286T3
ES2414286T3 ES08709576T ES08709576T ES2414286T3 ES 2414286 T3 ES2414286 T3 ES 2414286T3 ES 08709576 T ES08709576 T ES 08709576T ES 08709576 T ES08709576 T ES 08709576T ES 2414286 T3 ES2414286 T3 ES 2414286T3
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ES
Spain
Prior art keywords
primer
nucleic acid
nucleotide
enrichment
amplification
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ES08709576T
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English (en)
Spanish (es)
Inventor
Guoliang Fu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
360 Genomics Ltd
Oxitec Ltd
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360 Genomics Ltd
Oxitec Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
ES08709576T 2007-03-01 2008-02-29 Detección de ácidos nucleicos Active ES2414286T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0703996 2007-03-01
GB0703996A GB0703996D0 (en) 2007-03-01 2007-03-01 Nucleic acid detection
PCT/GB2008/000707 WO2008104794A2 (en) 2007-03-01 2008-02-29 Nucleic acid detection

Publications (1)

Publication Number Publication Date
ES2414286T3 true ES2414286T3 (es) 2013-07-18

Family

ID=37965755

Family Applications (1)

Application Number Title Priority Date Filing Date
ES08709576T Active ES2414286T3 (es) 2007-03-01 2008-02-29 Detección de ácidos nucleicos

Country Status (8)

Country Link
US (1) US9340832B2 (enExample)
EP (1) EP2129797B8 (enExample)
JP (1) JP5394937B2 (enExample)
CN (1) CN101680029B (enExample)
DK (1) DK2129797T3 (enExample)
ES (1) ES2414286T3 (enExample)
GB (1) GB0703996D0 (enExample)
WO (1) WO2008104794A2 (enExample)

Families Citing this family (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2071035A1 (en) * 2007-12-13 2009-06-17 Epigenomics AG Facilitator and method for amplification
US8709726B2 (en) 2008-03-11 2014-04-29 Sequenom, Inc. Nucleic acid-based tests for prenatal gender determination
US8962247B2 (en) 2008-09-16 2015-02-24 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses
US8476013B2 (en) 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
CN102301005A (zh) 2008-12-17 2011-12-28 生命技术公司 用于检测等位基因变体的方法、组合物和试剂盒
JP5805064B2 (ja) 2009-03-27 2015-11-04 ライフ テクノロジーズ コーポレーション 対立遺伝子変種を検出するための方法、組成物、およびキット
CN101633957A (zh) * 2009-06-26 2010-01-27 北大工学院绍兴技术研究院 用于检测小rna的方法及试剂盒
US9926593B2 (en) 2009-12-22 2018-03-27 Sequenom, Inc. Processes and kits for identifying aneuploidy
CN102985560A (zh) * 2010-03-30 2013-03-20 莫诺匡特私人有限公司 调控寡核苷酸功能性的方法
CN101871007B (zh) * 2010-05-07 2014-04-30 无锡锐奇基因生物科技有限公司 用标记探针进行检测和熔解曲线分析的方法
EP2598655B1 (en) * 2010-07-29 2016-10-05 F.Hoffmann-La Roche Ag Qualitative and quantitative detection of microbial nucleic acids
US9353406B2 (en) * 2010-10-22 2016-05-31 Fluidigm Corporation Universal probe assay methods
CA2834218C (en) 2011-04-29 2021-02-16 Sequenom, Inc. Quantification of a minority nucleic acid species using inhibitory oligonucleotides
WO2012151328A2 (en) 2011-05-02 2012-11-08 President And Fellows Of Harvard College Spatial sequestration of dynamic nucleic acid circuits
EP3382039B1 (en) * 2011-05-04 2023-09-13 Biocept, Inc. Methods for detecting nucleic acid sequence variants
JP5299981B1 (ja) * 2011-09-08 2013-09-25 独立行政法人理化学研究所 プライマーセット及びそれを用いた標的核酸配列の増幅方法並びに変異核酸の検出方法
GB201116131D0 (en) * 2011-09-19 2011-11-02 Epistem Ltd Probe
US20140377762A1 (en) * 2011-12-19 2014-12-25 360 Genomics Ltd. Method for enriching and detection of variant target nucleic acids
CN102559661B (zh) * 2012-01-18 2014-06-04 厦门基科生物科技有限公司 一种新型连接酶反应介导的扩增方法及用途
US9862994B2 (en) 2012-02-09 2018-01-09 Dana-Farber Cancer Institute, Inc. Selective nucleic acid amplification from nucleic acid pools
EP2820129A1 (en) 2012-03-02 2015-01-07 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US10504613B2 (en) 2012-12-20 2019-12-10 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
WO2014011928A1 (en) 2012-07-13 2014-01-16 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
ES3049590T3 (en) * 2012-11-02 2025-12-17 Life Technologies Corp Small rna capture, detection and quantification
US9222122B2 (en) 2012-11-20 2015-12-29 Src, Inc. System and method for rapid detection and identification of nucleic acid labeled tags
EP2971100A1 (en) 2013-03-13 2016-01-20 Sequenom, Inc. Primers for dna methylation analysis
GB201310584D0 (en) * 2013-06-13 2013-07-31 Base4 Innovation Ltd Droplet storage method
EP3022323B1 (en) * 2013-07-18 2019-04-24 President and Fellows of Harvard College Specific nucleic acid amplification with compounded selectivity
CN111128302B (zh) * 2013-11-26 2022-10-11 杭州联川基因诊断技术有限公司 一种设计pcr引物的方法
EP3736344A1 (en) 2014-03-13 2020-11-11 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
CN104677866A (zh) * 2014-03-25 2015-06-03 中国科学院大连化学物理研究所 测定CP装载siRNA体系中siRNA释放的方法
US9909167B2 (en) 2014-06-23 2018-03-06 The Board Of Trustees Of The Leland Stanford Junior University On-slide staining by primer extension
GB201412316D0 (en) 2014-07-10 2014-08-27 Momentum Bioscience Ltd Detecting the absence of micro-organisms
CN114250278B (zh) 2015-03-13 2024-08-06 生命技术公司 捕获、检测和定量小rna的方法、组合物与试剂盒
US20200087726A1 (en) * 2015-05-10 2020-03-19 Quandx Inc. Ultra sensitive probes for detection of nucleic acid
US20180127803A1 (en) * 2015-05-10 2018-05-10 Quandx Inc. Ultra sensitive probes for detection of nucleic acid
CN105274096A (zh) * 2015-09-07 2016-01-27 中国人民解放军第三军医大学第一附属医院 桥式序列区掺入错配碱基的桥式荧光探针及其应用和方法
CN105506082B (zh) * 2015-12-24 2019-04-09 杭州师范大学附属医院 利用链转移型引物扩增核酸及融合探针的方法
WO2018022809A1 (en) 2016-07-27 2018-02-01 The Board Of Trustees Of The Leland Stanford Junior University Highly-multiplexed fluorescent imaging
US10081829B1 (en) 2017-06-13 2018-09-25 Genetics Research, Llc Detection of targeted sequence regions
US10947599B2 (en) 2017-06-13 2021-03-16 Genetics Research, Llc Tumor mutation burden
EP3638813A4 (en) 2017-06-13 2021-06-02 Genetics Research, LLC, D/B/A ZS Genetics, Inc. ISOLATION OF TARGET NUCLEIC ACIDS
US10527608B2 (en) 2017-06-13 2020-01-07 Genetics Research, Llc Methods for rare event detection
CN107881218B (zh) * 2017-11-23 2021-09-28 中国科学院合肥物质科学研究院 一种用于端粒酶活性检测的球形核酸荧光探针及其制备方法和用途
CN108251502B (zh) * 2017-12-27 2021-12-24 深圳华大基因股份有限公司 一种外周血游离肿瘤dna的富集方法、试剂盒及其应用
CN110240999B (zh) * 2018-03-09 2022-09-06 浙江品级基因科技有限公司 一种提高循环肿瘤dna检出率的检测装置及方法
CA3102892A1 (en) 2018-06-12 2019-12-19 Keygene N.V. Nucleic acid amplification method
AU2019307835B2 (en) 2018-07-19 2021-08-19 Biofidelity Ltd Improved polynucleotide sequence detection method
US11921049B2 (en) * 2018-11-09 2024-03-05 Shaofeng Ding Pyrophosphorolysis activated fluorescence to measure PAP amplification of nucleic acid
CN109828120B (zh) * 2019-03-12 2021-07-30 南京大学 基于靶标激发金纳米粒子表面dna循环的蛋白质荧光分析方法
US20220372485A1 (en) * 2019-10-15 2022-11-24 University Of Florida Research Foundation, Incorporated Aptamer based system to quantify anti-thrombin iii in blood
GB201919186D0 (en) 2019-12-23 2020-02-05 Biofidelity Ltd Simplified polynucleotide sequence detection method
US20240150818A1 (en) * 2021-02-16 2024-05-09 Biofidelity Ltd Methods for polynucleotide detection
CN113234803A (zh) * 2021-06-17 2021-08-10 上海理工大学 一种新型核酸扩增方法

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2650840B1 (fr) 1989-08-11 1991-11-29 Bertin & Cie Procede rapide de detection et/ou d'identification d'une seule base sur une sequence d'acide nucleique, et ses applications
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
GB9211979D0 (en) * 1992-06-05 1992-07-15 Buchard Ole Uses of nucleic acid analogues
US6376178B1 (en) * 1993-09-03 2002-04-23 Duke University Method of nucleic acid sequencing
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US6174670B1 (en) * 1996-06-04 2001-01-16 University Of Utah Research Foundation Monitoring amplification of DNA during PCR
GB2333597B (en) * 1996-10-29 2001-04-18 Univ Nebraska At Lincoln Method for detecting point mutations in dna utilizing fluorescence energy transfer
US5849497A (en) * 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
FR2779154B1 (fr) 1998-05-27 2002-07-12 Bio Merieux Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre
GB9811415D0 (en) 1998-05-28 1998-07-22 Dedar Limited Gasifiers
GB9902971D0 (en) * 1999-02-11 1999-03-31 Zeneca Ltd Assay
AU3274500A (en) * 1999-03-18 2000-10-09 Exiqon A/S Detection of mutations in genes by specific lna primers
US20020025519A1 (en) 1999-06-17 2002-02-28 David J. Wright Methods and oligonucleotides for detecting nucleic acid sequence variations
EP1250452A1 (en) * 1999-12-02 2002-10-23 DNA Sciences, Inc. Methods for determining single nucleotide variations and genotyping
JP3638516B2 (ja) * 2000-09-28 2005-04-13 株式会社日立製作所 核酸検出方法および核酸検出キット
US6391592B1 (en) * 2000-12-14 2002-05-21 Affymetrix, Inc. Blocker-aided target amplification of nucleic acids
US8512953B2 (en) 2001-09-24 2013-08-20 One Lambda Diagnostic probe detection system
EP1481088B1 (en) * 2002-03-01 2008-09-17 Integrated Dna Technologies, Inc. Polynomial amplification of nucleic acids
US20030211483A1 (en) 2002-05-09 2003-11-13 Schroeder Benjamin G. Methods for the enrichment of low-abundance polynucleotides
EP1558759A4 (en) * 2002-07-01 2007-06-06 Univ State Cleveland PROCESS FOR DETECTING MUTATED POLYNUCLEOTIDES WITHIN A LARGE POPULATION OF WILD TYPE POLYNUCLEOTIDES
CA2494993A1 (en) * 2002-08-21 2004-03-04 Epoch Biosciences, Inc. Abasic site endonuclease assay
GB2395556A (en) 2002-11-22 2004-05-26 Dynal Biotech Ltd Nucleic acid probe
WO2004065628A1 (en) 2003-01-21 2004-08-05 Guoliang Fu Quantitative multiplex detection of nucleic acids
JP4608483B2 (ja) 2003-02-18 2011-01-12 アプライド バイオシステムズ， エルエルシー ポリヌクレオチドの複合的分析のための組成物および方法
EP1687437A4 (en) * 2003-10-13 2008-01-02 Genaco Biomedial Products Inc METHOD AND KIT FOR MULTIPLEX AMPLIFICATION OF NUCLEIC ACID PRIMERS
US20060183136A1 (en) * 2004-11-05 2006-08-17 Genevieve Pont-Kingdon Method for haplotyping and genotyping by melting curve analysis of hybridization probes
WO2007008997A2 (en) 2005-07-12 2007-01-18 The Board Of Trustees Of The Leland Stanford Junior University Template specific inhibition of pcr
WO2007045890A1 (en) 2005-10-21 2007-04-26 Primerdesign Ltd Probe
US20100009355A1 (en) 2006-03-14 2010-01-14 Kolodney Michael S Selective amplification of minority mutations using primer blocking high-affinity oligonucleotides
GB0703997D0 (en) * 2007-03-01 2007-04-11 Oxitec Ltd Methods for detecting nucleic sequences

Also Published As

Publication number Publication date
CN101680029B (zh) 2013-09-25
US9340832B2 (en) 2016-05-17
JP2010519897A (ja) 2010-06-10
WO2008104794A3 (en) 2008-11-20
GB0703996D0 (en) 2007-04-11
EP2129797B8 (en) 2013-05-15
CN101680029A (zh) 2010-03-24
DK2129797T3 (da) 2013-07-08
JP5394937B2 (ja) 2014-01-22
US20100227320A1 (en) 2010-09-09
WO2008104794A2 (en) 2008-09-04
EP2129797B1 (en) 2013-04-03
EP2129797A2 (en) 2009-12-09

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