ES2287989T3 - Clonacion de expresion en hongos filamentosos. - Google Patents
Clonacion de expresion en hongos filamentosos. Download PDFInfo
- Publication number
- ES2287989T3 ES2287989T3 ES98966868T ES98966868T ES2287989T3 ES 2287989 T3 ES2287989 T3 ES 2287989T3 ES 98966868 T ES98966868 T ES 98966868T ES 98966868 T ES98966868 T ES 98966868T ES 2287989 T3 ES2287989 T3 ES 2287989T3
- Authority
- ES
- Spain
- Prior art keywords
- baselineskip
- dna
- expression
- vector
- cloning
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 230000014509 gene expression Effects 0.000 title claims abstract description 76
- 241000233866 Fungi Species 0.000 title claims abstract description 38
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 118
- 108020004414 DNA Proteins 0.000 claims abstract description 64
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 57
- 238000000034 method Methods 0.000 claims abstract description 51
- 108091028043 Nucleic acid sequence Proteins 0.000 claims abstract description 32
- 239000013599 cloning vector Substances 0.000 claims abstract description 25
- 239000003550 marker Substances 0.000 claims abstract description 17
- 108700026220 vif Genes Proteins 0.000 claims abstract 2
- 239000013598 vector Substances 0.000 claims description 87
- 239000012634 fragment Substances 0.000 claims description 55
- 241000228245 Aspergillus niger Species 0.000 claims description 35
- 101150078331 ama-1 gene Proteins 0.000 claims description 23
- 102000004190 Enzymes Human genes 0.000 claims description 16
- 108090000790 Enzymes Proteins 0.000 claims description 16
- 241000228212 Aspergillus Species 0.000 claims description 15
- 241000351920 Aspergillus nidulans Species 0.000 claims description 11
- 240000006439 Aspergillus oryzae Species 0.000 claims description 4
- 241000499912 Trichoderma reesei Species 0.000 claims description 4
- 241000894007 species Species 0.000 claims description 4
- 241000223259 Trichoderma Species 0.000 claims description 3
- 238000012423 maintenance Methods 0.000 claims description 3
- 235000002247 Aspergillus oryzae Nutrition 0.000 claims description 2
- 241000131386 Aspergillus sojae Species 0.000 claims description 2
- 238000004458 analytical method Methods 0.000 abstract description 28
- 238000012258 culturing Methods 0.000 abstract description 4
- 239000002299 complementary DNA Substances 0.000 description 80
- 238000010367 cloning Methods 0.000 description 58
- 108091034117 Oligonucleotide Proteins 0.000 description 47
- 235000018102 proteins Nutrition 0.000 description 44
- 239000013612 plasmid Substances 0.000 description 43
- 108010011619 6-Phytase Proteins 0.000 description 38
- 210000004027 cell Anatomy 0.000 description 36
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 description 34
- 230000009466 transformation Effects 0.000 description 34
- 238000010276 construction Methods 0.000 description 33
- 229940085127 phytase Drugs 0.000 description 32
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 30
- 101150108358 GLAA gene Proteins 0.000 description 29
- 108020004635 Complementary DNA Proteins 0.000 description 28
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 26
- 239000013604 expression vector Substances 0.000 description 26
- 238000001514 detection method Methods 0.000 description 25
- 108010054576 Deoxyribonuclease EcoRI Proteins 0.000 description 22
- 108020004999 messenger RNA Proteins 0.000 description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 22
- 230000000694 effects Effects 0.000 description 21
- 239000000872 buffer Substances 0.000 description 20
- 241000588724 Escherichia coli Species 0.000 description 19
- 239000002609 medium Substances 0.000 description 19
- 239000000243 solution Substances 0.000 description 19
- 238000001400 expression cloning Methods 0.000 description 18
- 238000004519 manufacturing process Methods 0.000 description 18
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 17
- 238000006243 chemical reaction Methods 0.000 description 17
- 238000003786 synthesis reaction Methods 0.000 description 17
- DLFVBJFMPXGRIB-UHFFFAOYSA-N Acetamide Chemical compound CC(N)=O DLFVBJFMPXGRIB-UHFFFAOYSA-N 0.000 description 16
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 16
- AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 16
- 230000015572 biosynthetic process Effects 0.000 description 15
- 229940088598 enzyme Drugs 0.000 description 15
- 230000002538 fungal effect Effects 0.000 description 15
- 230000010354 integration Effects 0.000 description 14
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 13
- 230000029087 digestion Effects 0.000 description 13
- 238000011534 incubation Methods 0.000 description 12
- 239000000047 product Substances 0.000 description 12
- 238000003780 insertion Methods 0.000 description 11
- 230000037431 insertion Effects 0.000 description 11
- 238000012360 testing method Methods 0.000 description 11
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 10
- 238000000855 fermentation Methods 0.000 description 10
- 230000004151 fermentation Effects 0.000 description 10
- 238000000746 purification Methods 0.000 description 10
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 description 9
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 9
- 238000005119 centrifugation Methods 0.000 description 9
- 239000000203 mixture Substances 0.000 description 9
- 239000001965 potato dextrose agar Substances 0.000 description 9
- 210000001938 protoplast Anatomy 0.000 description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- 241000894006 Bacteria Species 0.000 description 8
- 102100022624 Glucoamylase Human genes 0.000 description 8
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 8
- 238000010804 cDNA synthesis Methods 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 8
- 238000009396 hybridization Methods 0.000 description 8
- 238000001556 precipitation Methods 0.000 description 8
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 7
- 238000001962 electrophoresis Methods 0.000 description 7
- 239000000499 gel Substances 0.000 description 7
- 238000002156 mixing Methods 0.000 description 7
- 239000008188 pellet Substances 0.000 description 7
- 239000006228 supernatant Substances 0.000 description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 6
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 238000003556 assay Methods 0.000 description 6
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 6
- 239000008103 glucose Substances 0.000 description 6
- 238000002955 isolation Methods 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 230000004048 modification Effects 0.000 description 6
- 238000012986 modification Methods 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 6
- 239000000725 suspension Substances 0.000 description 6
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 5
- 229920001817 Agar Polymers 0.000 description 5
- 108010048241 acetamidase Proteins 0.000 description 5
- 239000008272 agar Substances 0.000 description 5
- 230000001965 increasing effect Effects 0.000 description 5
- 230000001939 inductive effect Effects 0.000 description 5
- 239000011541 reaction mixture Substances 0.000 description 5
- 108091008146 restriction endonucleases Proteins 0.000 description 5
- 239000006152 selective media Substances 0.000 description 5
- 229920001221 xylan Polymers 0.000 description 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
- GHOKWGTUZJEAQD-ZETCQYMHSA-N (D)-(+)-Pantothenic acid Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-ZETCQYMHSA-N 0.000 description 4
- 241000228232 Aspergillus tubingensis Species 0.000 description 4
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 4
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 4
- 239000011543 agarose gel Substances 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 238000010805 cDNA synthesis kit Methods 0.000 description 4
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 4
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 4
- 238000010841 mRNA extraction Methods 0.000 description 4
- 230000001404 mediated effect Effects 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 235000004252 protein component Nutrition 0.000 description 4
- 230000002285 radioactive effect Effects 0.000 description 4
- 230000028327 secretion Effects 0.000 description 4
- 239000011734 sodium Substances 0.000 description 4
- 239000000600 sorbitol Substances 0.000 description 4
- 150000004823 xylans Chemical class 0.000 description 4
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 3
- 108010008885 Cellulose 1,4-beta-Cellobiosidase Proteins 0.000 description 3
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 3
- 241000206602 Eukaryota Species 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
- 108091026898 Leader sequence (mRNA) Proteins 0.000 description 3
- 239000007993 MOPS buffer Substances 0.000 description 3
- 108091092724 Noncoding DNA Proteins 0.000 description 3
- 229930182555 Penicillin Natural products 0.000 description 3
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 3
- 238000012300 Sequence Analysis Methods 0.000 description 3
- 229930006000 Sucrose Natural products 0.000 description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 239000001110 calcium chloride Substances 0.000 description 3
- 235000011148 calcium chloride Nutrition 0.000 description 3
- 229910001628 calcium chloride Inorganic materials 0.000 description 3
- 229940041514 candida albicans extract Drugs 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 238000011049 filling Methods 0.000 description 3
- 238000005194 fractionation Methods 0.000 description 3
- 101150073906 gpdA gene Proteins 0.000 description 3
- 238000002372 labelling Methods 0.000 description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 3
- 235000019341 magnesium sulphate Nutrition 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 238000010369 molecular cloning Methods 0.000 description 3
- 230000035772 mutation Effects 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 150000007523 nucleic acids Chemical class 0.000 description 3
- 102000039446 nucleic acids Human genes 0.000 description 3
- 229940049954 penicillin Drugs 0.000 description 3
- 230000026731 phosphorylation Effects 0.000 description 3
- 238000006366 phosphorylation reaction Methods 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 239000008223 sterile water Substances 0.000 description 3
- 229960005322 streptomycin Drugs 0.000 description 3
- 239000005720 sucrose Substances 0.000 description 3
- 239000012138 yeast extract Substances 0.000 description 3
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 2
- 239000004382 Amylase Substances 0.000 description 2
- 108010059892 Cellulase Proteins 0.000 description 2
- 108010084185 Cellulases Proteins 0.000 description 2
- 102000005575 Cellulases Human genes 0.000 description 2
- GHOKWGTUZJEAQD-UHFFFAOYSA-N Chick antidermatitis factor Natural products OCC(C)(C)C(O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-UHFFFAOYSA-N 0.000 description 2
- 229910021580 Cobalt(II) chloride Inorganic materials 0.000 description 2
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- 108700005088 Fungal Genes Proteins 0.000 description 2
- 108090000364 Ligases Proteins 0.000 description 2
- 102000003960 Ligases Human genes 0.000 description 2
- 238000000636 Northern blotting Methods 0.000 description 2
- 102000004316 Oxidoreductases Human genes 0.000 description 2
- 108090000854 Oxidoreductases Proteins 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 102000035195 Peptidases Human genes 0.000 description 2
- 241000235648 Pichia Species 0.000 description 2
- 229920001030 Polyethylene Glycol 4000 Polymers 0.000 description 2
- 108010076504 Protein Sorting Signals Proteins 0.000 description 2
- 108091034057 RNA (poly(A)) Proteins 0.000 description 2
- 238000002123 RNA extraction Methods 0.000 description 2
- 108020004511 Recombinant DNA Proteins 0.000 description 2
- 229920002684 Sepharose Polymers 0.000 description 2
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 101100370749 Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) trpC1 gene Proteins 0.000 description 2
- DFPAKSUCGFBDDF-ZQBYOMGUSA-N [14c]-nicotinamide Chemical compound N[14C](=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-ZQBYOMGUSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 2
- 125000003275 alpha amino acid group Chemical group 0.000 description 2
- ROOXNKNUYICQNP-UHFFFAOYSA-N ammonium persulfate Chemical compound [NH4+].[NH4+].[O-]S(=O)(=O)OOS([O-])(=O)=O ROOXNKNUYICQNP-UHFFFAOYSA-N 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 229960002685 biotin Drugs 0.000 description 2
- 235000020958 biotin Nutrition 0.000 description 2
- 239000011616 biotin Substances 0.000 description 2
- 235000010633 broth Nutrition 0.000 description 2
- 239000005018 casein Substances 0.000 description 2
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 2
- 235000021240 caseins Nutrition 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 239000012149 elution buffer Substances 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 102000006602 glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 2
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 2
- 239000010931 gold Substances 0.000 description 2
- 229910052737 gold Inorganic materials 0.000 description 2
- 101150095733 gpsA gene Proteins 0.000 description 2
- 230000005484 gravity Effects 0.000 description 2
- 239000012145 high-salt buffer Substances 0.000 description 2
- PHTQWCKDNZKARW-UHFFFAOYSA-N isoamylol Chemical compound CC(C)CCO PHTQWCKDNZKARW-UHFFFAOYSA-N 0.000 description 2
- -1 mananasas Proteins 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 230000003204 osmotic effect Effects 0.000 description 2
- 229940055726 pantothenic acid Drugs 0.000 description 2
- 235000019161 pantothenic acid Nutrition 0.000 description 2
- 239000011713 pantothenic acid Substances 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- 238000002331 protein detection Methods 0.000 description 2
- 238000003908 quality control method Methods 0.000 description 2
- 238000000163 radioactive labelling Methods 0.000 description 2
- 230000008929 regeneration Effects 0.000 description 2
- 238000011069 regeneration method Methods 0.000 description 2
- 229960002477 riboflavin Drugs 0.000 description 2
- 235000019192 riboflavin Nutrition 0.000 description 2
- 239000002151 riboflavin Substances 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 239000012723 sample buffer Substances 0.000 description 2
- 239000001632 sodium acetate Substances 0.000 description 2
- 235000017281 sodium acetate Nutrition 0.000 description 2
- FQENQNTWSFEDLI-UHFFFAOYSA-J sodium diphosphate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]P([O-])(=O)OP([O-])([O-])=O FQENQNTWSFEDLI-UHFFFAOYSA-J 0.000 description 2
- 229940048086 sodium pyrophosphate Drugs 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 230000008685 targeting Effects 0.000 description 2
- 235000019818 tetrasodium diphosphate Nutrition 0.000 description 2
- 239000001577 tetrasodium phosphonato phosphate Substances 0.000 description 2
- 229960003495 thiamine Drugs 0.000 description 2
- 230000014616 translation Effects 0.000 description 2
- 101150016309 trpC gene Proteins 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- 229940011671 vitamin b6 Drugs 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 2
- 229910000368 zinc sulfate Inorganic materials 0.000 description 2
- 239000011686 zinc sulphate Substances 0.000 description 2
- 235000009529 zinc sulphate Nutrition 0.000 description 2
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 1
- 241001019659 Acremonium <Plectosphaerellaceae> Species 0.000 description 1
- 102000007698 Alcohol dehydrogenase Human genes 0.000 description 1
- 108010021809 Alcohol dehydrogenase Proteins 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 101710152845 Arabinogalactan endo-beta-1,4-galactanase Proteins 0.000 description 1
- 241000228195 Aspergillus ficuum Species 0.000 description 1
- 101000757144 Aspergillus niger Glucoamylase Proteins 0.000 description 1
- 101150076489 B gene Proteins 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 108700038091 Beta-glucanases Proteins 0.000 description 1
- 0 CCCCCCCC(C1)[C@@](C)[C@@]1(*)[C@@](*)C(*)C(*)([C@](*)(CC1)C[C@@]1(*)C=C(C1)[C@@](*)C1C(*)[C@@](C)C(*=C)=CCC(*)CCCCCCCCC*#I(C)=C)c1ccccc1 Chemical compound CCCCCCCC(C1)[C@@](C)[C@@]1(*)[C@@](*)C(*)C(*)([C@](*)(CC1)C[C@@]1(*)C=C(C1)[C@@](*)C1C(*)[C@@](C)C(*=C)=CCC(*)CCCCCCCCC*#I(C)=C)c1ccccc1 0.000 description 1
- 241000221955 Chaetomium Species 0.000 description 1
- 241001503016 Ctenomyces Species 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 108010017826 DNA Polymerase I Proteins 0.000 description 1
- 102000004594 DNA Polymerase I Human genes 0.000 description 1
- 238000007399 DNA isolation Methods 0.000 description 1
- 101710132690 Endo-1,4-beta-xylanase A Proteins 0.000 description 1
- 101710147028 Endo-beta-1,4-galactanase Proteins 0.000 description 1
- 108090000371 Esterases Proteins 0.000 description 1
- 108010058643 Fungal Proteins Proteins 0.000 description 1
- 241000223218 Fusarium Species 0.000 description 1
- 108010093031 Galactosidases Proteins 0.000 description 1
- 102000002464 Galactosidases Human genes 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- 101100295959 Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) arcB gene Proteins 0.000 description 1
- GRRNUXAQVGOGFE-UHFFFAOYSA-N Hygromycin-B Natural products OC1C(NC)CC(N)C(O)C1OC1C2OC3(C(C(O)C(O)C(C(N)CO)O3)O)OC2C(O)C(CO)O1 GRRNUXAQVGOGFE-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 description 1
- 108091092195 Intron Proteins 0.000 description 1
- 102000004195 Isomerases Human genes 0.000 description 1
- 108090000769 Isomerases Proteins 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- 241000235649 Kluyveromyces Species 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 108060004795 Methyltransferase Proteins 0.000 description 1
- KWYHDKDOAIKMQN-UHFFFAOYSA-N N,N,N',N'-tetramethylethylenediamine Chemical compound CN(C)CCN(C)C KWYHDKDOAIKMQN-UHFFFAOYSA-N 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 1
- 238000010222 PCR analysis Methods 0.000 description 1
- 101150048253 PHYA gene Proteins 0.000 description 1
- 241000228143 Penicillium Species 0.000 description 1
- 241001442654 Percnon planissimum Species 0.000 description 1
- 108010002747 Pfu DNA polymerase Proteins 0.000 description 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 239000013614 RNA sample Substances 0.000 description 1
- 101150010882 S gene Proteins 0.000 description 1
- 241000235070 Saccharomyces Species 0.000 description 1
- 241000223255 Scytalidium Species 0.000 description 1
- 102000040739 Secretory proteins Human genes 0.000 description 1
- 108091058545 Secretory proteins Proteins 0.000 description 1
- 239000012506 Sephacryl® Substances 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 241001085826 Sporotrichum Species 0.000 description 1
- 241000228341 Talaromyces Species 0.000 description 1
- 241000228178 Thermoascus Species 0.000 description 1
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 1
- 241001494489 Thielavia Species 0.000 description 1
- ZMZDMBWJUHKJPS-UHFFFAOYSA-M Thiocyanate anion Chemical compound [S-]C#N ZMZDMBWJUHKJPS-UHFFFAOYSA-M 0.000 description 1
- 102000004357 Transferases Human genes 0.000 description 1
- 108090000992 Transferases Proteins 0.000 description 1
- 239000007984 Tris EDTA buffer Substances 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- 241001105470 Valenzuela Species 0.000 description 1
- 108010027199 Xylosidases Proteins 0.000 description 1
- 241000235013 Yarrowia Species 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 230000004308 accommodation Effects 0.000 description 1
- 239000008351 acetate buffer Substances 0.000 description 1
- YBCVMFKXIKNREZ-UHFFFAOYSA-N acoh acetic acid Chemical compound CC(O)=O.CC(O)=O YBCVMFKXIKNREZ-UHFFFAOYSA-N 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 102000004139 alpha-Amylases Human genes 0.000 description 1
- 108090000637 alpha-Amylases Proteins 0.000 description 1
- 229940024171 alpha-amylase Drugs 0.000 description 1
- 229910001870 ammonium persulfate Inorganic materials 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 230000003625 amylolytic effect Effects 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- 101150008194 argB gene Proteins 0.000 description 1
- 230000000721 bacterilogical effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 101150038738 ble gene Proteins 0.000 description 1
- UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical class C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 1
- 239000000337 buffer salt Substances 0.000 description 1
- 108010089934 carbohydrase Proteins 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- ZYWFEOZQIUMEGL-UHFFFAOYSA-N chloroform;3-methylbutan-1-ol;phenol Chemical compound ClC(Cl)Cl.CC(C)CCO.OC1=CC=CC=C1 ZYWFEOZQIUMEGL-UHFFFAOYSA-N 0.000 description 1
- 239000013611 chromosomal DNA Substances 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 101150081158 crtB gene Proteins 0.000 description 1
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical class O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
- 238000003936 denaturing gel electrophoresis Methods 0.000 description 1
- 239000008121 dextrose Substances 0.000 description 1
- XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 description 1
- 235000011180 diphosphates Nutrition 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 239000013613 expression plasmid Substances 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000011152 fibreglass Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 238000012252 genetic analysis Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 102000035122 glycosylated proteins Human genes 0.000 description 1
- 108091005608 glycosylated proteins Proteins 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 125000001475 halogen functional group Chemical group 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 108010002430 hemicellulase Proteins 0.000 description 1
- 230000006801 homologous recombination Effects 0.000 description 1
- 238000002744 homologous recombination Methods 0.000 description 1
- IXCSERBJSXMMFS-UHFFFAOYSA-N hydrogen chloride Substances Cl.Cl IXCSERBJSXMMFS-UHFFFAOYSA-N 0.000 description 1
- ZMZDMBWJUHKJPS-UHFFFAOYSA-N hydrogen thiocyanate Natural products SC#N ZMZDMBWJUHKJPS-UHFFFAOYSA-N 0.000 description 1
- GRRNUXAQVGOGFE-NZSRVPFOSA-N hygromycin B Chemical compound O[C@@H]1[C@@H](NC)C[C@@H](N)[C@H](O)[C@H]1O[C@H]1[C@H]2O[C@@]3([C@@H]([C@@H](O)[C@@H](O)[C@@H](C(N)CO)O3)O)O[C@H]2[C@@H](O)[C@@H](CO)O1 GRRNUXAQVGOGFE-NZSRVPFOSA-N 0.000 description 1
- 229940097277 hygromycin b Drugs 0.000 description 1
- 235000015243 ice cream Nutrition 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 239000012160 loading buffer Substances 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 101150039489 lysZ gene Proteins 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 239000004570 mortar (masonry) Substances 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 description 1
- 108010090409 novozym 234 Proteins 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 229940046166 oligodeoxynucleotide Drugs 0.000 description 1
- 239000002751 oligonucleotide probe Substances 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000002351 pectolytic effect Effects 0.000 description 1
- 235000002949 phytic acid Nutrition 0.000 description 1
- 239000000467 phytic acid Substances 0.000 description 1
- 229940068041 phytic acid Drugs 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000004952 protein activity Effects 0.000 description 1
- 230000012743 protein tagging Effects 0.000 description 1
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
- 101150054232 pyrG gene Proteins 0.000 description 1
- 235000008160 pyridoxine Nutrition 0.000 description 1
- 239000011677 pyridoxine Substances 0.000 description 1
- 235000019171 pyridoxine hydrochloride Nutrition 0.000 description 1
- 239000011764 pyridoxine hydrochloride Substances 0.000 description 1
- 229940048084 pyrophosphate Drugs 0.000 description 1
- 239000012857 radioactive material Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- KUIXZSYWBHSYCN-UHFFFAOYSA-L remazol brilliant blue r Chemical compound [Na+].[Na+].C1=C(S([O-])(=O)=O)C(N)=C2C(=O)C3=CC=CC=C3C(=O)C2=C1NC1=CC=CC(S(=O)(=O)CCOS([O-])(=O)=O)=C1 KUIXZSYWBHSYCN-UHFFFAOYSA-L 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 238000005204 segregation Methods 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000007974 sodium acetate buffer Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 229940079862 sodium lauryl sarcosinate Drugs 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
- ADWNFGORSPBALY-UHFFFAOYSA-M sodium;2-[dodecyl(methyl)amino]acetate Chemical compound [Na+].CCCCCCCCCCCCN(C)CC([O-])=O ADWNFGORSPBALY-UHFFFAOYSA-M 0.000 description 1
- 238000009987 spinning Methods 0.000 description 1
- 235000019157 thiamine Nutrition 0.000 description 1
- 239000011721 thiamine Substances 0.000 description 1
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 235000019190 thiamine hydrochloride Nutrition 0.000 description 1
- 239000011747 thiamine hydrochloride Substances 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 230000009105 vegetative growth Effects 0.000 description 1
- 239000011592 zinc chloride Substances 0.000 description 1
- 235000005074 zinc chloride Nutrition 0.000 description 1
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Mycology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Mushroom Cultivation (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP97204079 | 1997-12-22 | ||
| EP97204079 | 1997-12-22 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2287989T3 true ES2287989T3 (es) | 2007-12-16 |
Family
ID=8229117
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES98966868T Expired - Lifetime ES2287989T3 (es) | 1997-12-22 | 1998-12-22 | Clonacion de expresion en hongos filamentosos. |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US20020155536A1 (cg-RX-API-DMAC7.html) |
| EP (2) | EP1854890A1 (cg-RX-API-DMAC7.html) |
| JP (3) | JP4410413B2 (cg-RX-API-DMAC7.html) |
| CN (1) | CN1198924C (cg-RX-API-DMAC7.html) |
| AT (1) | ATE367439T1 (cg-RX-API-DMAC7.html) |
| AU (1) | AU736111C (cg-RX-API-DMAC7.html) |
| BR (1) | BR9814404A (cg-RX-API-DMAC7.html) |
| CA (1) | CA2313445C (cg-RX-API-DMAC7.html) |
| DE (1) | DE69838106T2 (cg-RX-API-DMAC7.html) |
| DK (1) | DK1042461T3 (cg-RX-API-DMAC7.html) |
| ES (1) | ES2287989T3 (cg-RX-API-DMAC7.html) |
| PL (1) | PL341760A1 (cg-RX-API-DMAC7.html) |
| WO (1) | WO1999032617A2 (cg-RX-API-DMAC7.html) |
Families Citing this family (66)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7883872B2 (en) | 1996-10-10 | 2011-02-08 | Dyadic International (Usa), Inc. | Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose |
| ATE376057T1 (de) | 1999-10-01 | 2007-11-15 | Novozymes As | Enzymgranulat |
| KR20020026456A (ko) | 2000-04-13 | 2002-04-10 | 에말파브 마크 아론 | 사상균에서 발현된 ｄｎａ 라이브러리의 고산출량 스크리닝 |
| DE60138947D1 (de) * | 2000-04-13 | 2009-07-23 | Dyadic Internat Usa Inc | Durchmusterung von bibliotheken exprimierter dna in filamentösen pilzen mit hoher durchsatzrate |
| AU7779800A (en) | 2000-09-21 | 2002-04-02 | Dsm Nv | Talaromyces xylanases |
| MXPA03005128A (es) | 2000-12-07 | 2004-12-06 | Dsm Ip Assets Bv | Hidrolizados proteicos enriquecidos en peptidos que tienen un residuo carboxiterminal de prolina. |
| WO2003054186A1 (en) | 2001-12-21 | 2003-07-03 | Dsm Ip Assets B.V. | New rennets |
| TWI328612B (en) | 2002-07-25 | 2010-08-11 | Dsm Ip Assets Bv | Genes and their encoded polypeptides involved in the biosynthetic pathway of vitamin b12, vectors and host cells comprising the genes, and process for producing vitamin b12 |
| WO2004078919A2 (en) | 2003-02-27 | 2004-09-16 | Midwest Research Institute | Superactive cellulase formulation using cellobiohydrolase-1 from penicillium funiculosum |
| US20050059024A1 (en) | 2003-07-25 | 2005-03-17 | Ambion, Inc. | Methods and compositions for isolating small RNA molecules |
| CA2560205A1 (en) * | 2004-04-16 | 2005-10-27 | Dsm Ip Assets B.V. | Fungal promoters for expressing a gene in a fungal cell |
| CA2565888C (en) | 2004-05-27 | 2014-10-14 | Novozymes, Inc. | Methods for transforming and expression screening of filamentous fungal cells with a dna library |
| EA014853B1 (ru) * | 2004-10-15 | 2011-02-28 | ДСМ АйПи АССЕТС Б.В. | Амидаза из aspergillus niger и применение амидазы для получения пищевого продукта с пониженным содержанием акриламида |
| CN101107354B (zh) | 2005-01-24 | 2012-05-30 | 帝斯曼知识产权资产管理有限公司 | 用于在有丝真菌细胞中生产感兴趣化合物的方法 |
| EP1859036A2 (en) * | 2005-03-18 | 2007-11-28 | DSMIP Assets B.V. | Method of expression cloning in a host cell |
| EP1954812B1 (en) | 2005-11-29 | 2012-11-21 | DSM IP Assets B.V. | Dna binding site of a transcriptional activator useful in gene expression |
| WO2007063133A2 (en) | 2005-12-01 | 2007-06-07 | Dsm Ip Assets B.V. | Genes useful for the industrial production of citric acid |
| CA2657975A1 (en) | 2006-06-29 | 2008-01-03 | Dsm Ip Assets B.V. | A method for achieving improved polypeptide expression |
| WO2008053018A2 (en) | 2006-11-02 | 2008-05-08 | Dsm Ip Assets B.V. | Improved production of secreted proteins by filamentous fungi |
| WO2008073914A2 (en) | 2006-12-10 | 2008-06-19 | Dyadic International Inc. | Expression and high-throughput screening of complex expressed dna libraries in filamentous fungi |
| WO2008098933A1 (en) | 2007-02-15 | 2008-08-21 | Dsm Ip Assets B.V. | A recombinant host cell for the production of a compound of interest |
| PL2113027T3 (pl) | 2007-02-20 | 2014-11-28 | Dsm Ip Assets Bv | Nowa sialidaza |
| EP2197893B1 (en) | 2007-09-07 | 2013-07-24 | Dyadic International, Inc. | Novel fungal enzymes |
| US8703463B2 (en) | 2009-03-10 | 2014-04-22 | Dsm Ip Assets B.V. | Pregastric esterase and derivatives thereof |
| EP2406386B1 (en) | 2009-03-10 | 2019-01-23 | DSM IP Assets B.V. | Method for improving the yield of a polypeptide |
| WO2010121933A1 (en) | 2009-04-22 | 2010-10-28 | Dsm Ip Assets B.V. | Process for the production of a recombinant polypeptide of interest |
| US8734782B2 (en) | 2009-07-22 | 2014-05-27 | Dsm Ip Assets B.V. | Host cell for the production of a compound of interest |
| CA2779796A1 (en) | 2009-11-04 | 2011-05-12 | Dsm Ip Assets B.V. | Talaromyces transformants |
| CN103347997A (zh) | 2010-02-04 | 2013-10-09 | 帝斯曼知识产权资产管理有限公司 | 制备具有性周期的丝状真菌菌株的方法和制备有性杂交丝状真菌菌株的方法 |
| EA201201125A1 (ru) | 2010-02-11 | 2013-03-29 | ДСМ АйПи АССЕТС Б.В. | Клетка-хозяин, способная продуцировать ферменты, пригодные для деградации лигноцеллюлозного материала |
| AU2011273351A1 (en) | 2010-07-01 | 2013-01-17 | Dsm Ip Assets B.V. | A method for the production of a compound of interest |
| WO2012007446A1 (en) | 2010-07-13 | 2012-01-19 | Dsm Ip Assets B.V. | Use of a mutarotase in the production of dried powders |
| WO2012007445A1 (en) | 2010-07-13 | 2012-01-19 | Dsm Ip Assets B.V. | Mutarotase in crystallization |
| CN104508114A (zh) | 2012-06-19 | 2015-04-08 | 帝斯曼知识产权资产管理有限公司 | 用于在细胞中表达基因的启动子 |
| DK3327112T3 (en) | 2012-07-19 | 2020-05-04 | Dsm Ip Assets Bv | Agse-deficient stamme |
| KR102134498B1 (ko) | 2013-02-04 | 2020-07-16 | 디에스엠 아이피 어셋츠 비.브이. | 탄수화물 분해 폴리펩티드 및 이의 용도 |
| WO2014142647A1 (en) | 2013-03-14 | 2014-09-18 | Wageningen Universiteit | Fungals strains with improved citric acid and itaconic acid production |
| WO2014202624A2 (en) | 2013-06-19 | 2014-12-24 | Dsm Ip Assets B.V. | Rasamsonia gene and use thereof |
| WO2014202622A2 (en) | 2013-06-19 | 2014-12-24 | Dsm Ip Assets B.V. | Rasamsonia gene and use thereof |
| WO2014202620A2 (en) | 2013-06-19 | 2014-12-24 | Dsm Ip Assets B.V. | Rasamsonia gene and use thereof |
| WO2014202621A1 (en) | 2013-06-20 | 2014-12-24 | Dsm Ip Assets B.V. | Carbohydrate degrading polypeptide and uses thereof |
| ES2706742T3 (es) | 2013-12-02 | 2019-04-01 | Dsm Ip Assets Bv | Proteína estructuradora del hielo |
| ES2773889T3 (es) * | 2014-01-31 | 2020-07-15 | Api Corp | Hidroxilasa en la posición 4 de ácido pipecolínico y método para producir 4-hidroxiaminoácido usando la misma |
| WO2015177171A1 (en) | 2014-05-19 | 2015-11-26 | Dsm Ip Assets B.V. | Proline-specific endoprotease |
| WO2015177153A1 (en) | 2014-05-19 | 2015-11-26 | Dsm Ip Assets B.V. | Proline-specific endoprotease |
| WO2015177152A1 (en) | 2014-05-19 | 2015-11-26 | Dsm Ip Assets B.V. | Proline-specific endoprotease |
| EP3242950B1 (en) | 2015-01-06 | 2021-10-06 | DSM IP Assets B.V. | A crispr-cas system for a filamentous fungal host cell |
| AR104205A1 (es) | 2015-04-09 | 2017-07-05 | Dsm Ip Assets Bv | Fosfolipasa c |
| US20180160695A1 (en) | 2015-06-02 | 2018-06-14 | Dsm Ip Assets B.V. | Use of ice structuring protein afp19 expressed in filamentous fungal strains for preparing food |
| EP4361240A3 (en) | 2015-11-17 | 2024-07-31 | DSM IP Assets B.V. | Preparation of a stable beer |
| US10913938B2 (en) | 2016-07-29 | 2021-02-09 | Dsm Ip Assets B.V. | Polypeptides having cellulolytic enhancing activity and uses thereof |
| SI3296401T1 (sl) * | 2016-09-20 | 2020-07-31 | Clariant International Ltd | Metoda za selektivno vir ogljika-neodvisno ekspresijo protein-kodirajočih sekvenc v celici filamentozne glive |
| WO2017216392A1 (en) | 2016-09-23 | 2017-12-21 | Dsm Ip Assets B.V. | A guide-rna expression system for a host cell |
| EP3559221A1 (en) | 2016-12-21 | 2019-10-30 | DSM IP Assets B.V. | Lipolytic enzyme variants |
| US10918113B2 (en) | 2016-12-21 | 2021-02-16 | Dsm Ip Assets B.V. | Lipolytic enzyme variants |
| WO2018114938A1 (en) | 2016-12-21 | 2018-06-28 | Dsm Ip Assets B.V. | Lipolytic enzyme variants |
| WO2018114912A1 (en) | 2016-12-21 | 2018-06-28 | Dsm Ip Assets B.V. | Lipolytic enzyme variants |
| CN110431224A (zh) | 2017-03-13 | 2019-11-08 | 帝斯曼知识产权资产管理有限公司 | 锌双核簇转录调控因子缺陷型菌株 |
| CN110462044A (zh) | 2017-04-06 | 2019-11-15 | 帝斯曼知识产权资产管理有限公司 | 自引导整合构建体(sgic) |
| WO2019215078A1 (en) | 2018-05-07 | 2019-11-14 | Dsm Ip Assets B.V. | Process for enzymatic oil degumming |
| MX2020014191A (es) | 2018-06-19 | 2021-03-09 | Dsm Ip Assets Bv | Variantes de enzimas lipoliticas. |
| US20220389458A1 (en) | 2019-11-04 | 2022-12-08 | Dsm Ip Assets B.V. | Low volume transfection |
| JP2021093966A (ja) * | 2019-12-18 | 2021-06-24 | 本田技研工業株式会社 | 糸状菌への遺伝子導入方法及びその利用 |
| CA3225417A1 (en) | 2021-06-24 | 2022-12-29 | Fonterra Co-Operative Group Limited | Recombinant proteins |
| JP2025520009A (ja) | 2022-05-16 | 2025-07-01 | ディーエスエム アイピー アセッツ ビー．ブイ． | 脂肪分解酵素変異体 |
| WO2025012325A1 (en) | 2023-07-11 | 2025-01-16 | Dsm Ip Assets B.V. | Protein arginine deiminase |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK122686D0 (da) * | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
| WO1990015860A1 (en) * | 1989-06-16 | 1990-12-27 | Genencor, Inc. | Dna sequences, vectors, and fusion polypeptides to increase secretion of desired polypeptides from filamentous fungi |
| CA2063592C (en) * | 1989-07-07 | 2003-01-21 | Marco L. F. Giuseppin | Process for preparing a protein by a fungus transformed by multicopy integration of an expression vector |
| JPH0595787A (ja) * | 1991-10-04 | 1993-04-20 | Jozo Shigen Kenkyusho:Kk | ホスホグリセリン酸キナ−ゼ遺伝子プロモ−タ−、 同タ−ミネ−タ−または／及びこれらからなる 遺伝子発現用ユニツト、並びに、ホスホグリセリ ン酸キナ−ゼのゲノム遺伝子及びｃＤＮＡ遺伝子 |
| MX9206979A (es) * | 1991-12-04 | 1993-07-01 | Novo Nordisk As | Metodo de clonacion de proteinas en levaduras |
| JPH05268964A (ja) * | 1992-03-30 | 1993-10-19 | Meiji Milk Prod Co Ltd | 糸状菌プロモーター |
| ES2198410T3 (es) * | 1993-07-23 | 2004-02-01 | Dsm Ip Assets B.V. | Cepas recombinantes de hongos filamentosos libres de gen marcador de seleccion: un metodo para obtener dichas cepas y su utilizacion. |
| JP3638657B2 (ja) * | 1995-03-29 | 2005-04-13 | 株式会社資生堂 | Ｄｎａ修復酵素 |
| EP0822990A4 (en) * | 1995-04-24 | 2002-07-03 | Chromaxome Corp | METHODS OF GENERATING AND SCREENING NEW METABOLIC PATHWAYS |
| JP3343567B2 (ja) * | 1995-06-29 | 2002-11-11 | 大関株式会社 | 糸状菌のエンハンサーｄｎａ塩基配列およびその用途 |
| CN101070537A (zh) * | 1996-01-10 | 2007-11-14 | 诺沃奇梅兹有限公司 | 用于在细胞中体内产生突变体文库方法 |
-
1998
- 1998-12-22 ES ES98966868T patent/ES2287989T3/es not_active Expired - Lifetime
- 1998-12-22 EP EP07112086A patent/EP1854890A1/en not_active Withdrawn
- 1998-12-22 CA CA2313445A patent/CA2313445C/en not_active Expired - Fee Related
- 1998-12-22 AU AU25151/99A patent/AU736111C/en not_active Ceased
- 1998-12-22 DK DK98966868T patent/DK1042461T3/da active
- 1998-12-22 DE DE69838106T patent/DE69838106T2/de not_active Expired - Lifetime
- 1998-12-22 AT AT98966868T patent/ATE367439T1/de active
- 1998-12-22 JP JP2000525536A patent/JP4410413B2/ja not_active Expired - Fee Related
- 1998-12-22 WO PCT/EP1998/008577 patent/WO1999032617A2/en not_active Ceased
- 1998-12-22 CN CN98812563.3A patent/CN1198924C/zh not_active Expired - Lifetime
- 1998-12-22 BR BR9814404-9A patent/BR9814404A/pt not_active Application Discontinuation
- 1998-12-22 EP EP98966868A patent/EP1042461B1/en not_active Revoked
- 1998-12-22 PL PL98341760A patent/PL341760A1/xx unknown
-
2001
- 2001-11-05 US US09/993,164 patent/US20020155536A1/en active Pending
-
2005
- 2005-05-25 JP JP2005152703A patent/JP2005261438A/ja active Pending
-
2009
- 2009-10-20 JP JP2009241780A patent/JP2010046080A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| DE69838106T2 (de) | 2008-04-03 |
| CA2313445A1 (en) | 1999-07-01 |
| EP1042461B1 (en) | 2007-07-18 |
| EP1042461A2 (en) | 2000-10-11 |
| BR9814404A (pt) | 2000-10-10 |
| US20020155536A1 (en) | 2002-10-24 |
| DK1042461T3 (da) | 2007-09-24 |
| JP4410413B2 (ja) | 2010-02-03 |
| AU736111B2 (en) | 2001-07-26 |
| PL341760A1 (en) | 2001-05-07 |
| ATE367439T1 (de) | 2007-08-15 |
| WO1999032617A2 (en) | 1999-07-01 |
| WO1999032617A3 (en) | 1999-09-10 |
| JP2002509698A (ja) | 2002-04-02 |
| AU2515199A (en) | 1999-07-12 |
| CN1198924C (zh) | 2005-04-27 |
| JP2010046080A (ja) | 2010-03-04 |
| EP1854890A1 (en) | 2007-11-14 |
| CN1283227A (zh) | 2001-02-07 |
| CA2313445C (en) | 2012-03-20 |
| JP2005261438A (ja) | 2005-09-29 |
| AU736111C (en) | 2004-02-19 |
| DE69838106D1 (de) | 2007-08-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2287989T3 (es) | Clonacion de expresion en hongos filamentosos. | |
| CN102224245B (zh) | 在丝状真菌细胞中使用阳性和阴性选择性基因的方法 | |
| JP5043254B2 (ja) | 糸状面細胞内の問題のｄｎａライブラリーの作製及びスクリーニング | |
| US11149268B2 (en) | Assembly system for a eukaryotic cell | |
| US8067157B2 (en) | Expression cloning in filamentous fungi | |
| CN104603273B (zh) | 重组系统 | |
| US8883482B2 (en) | Methods for increasing homologous recombination of a nucleic acid sequence | |
| US20140106398A1 (en) | Vector-host system | |
| IE55817B1 (en) | Cloning system for kluyveromyces species | |
| CN100480391C (zh) | 用于在真菌细胞中表达基因的启动子变体 | |
| JP2021521821A (ja) | 粘性が低下した表現型を含む糸状菌株 | |
| WO2001040489A1 (en) | Methods for producing a polypeptide using a consensus translational initiator sequence | |
| JP2002515252A (ja) | 糸状菌変異体細胞においてポリペプチドを生産するための方法 | |
| US20030199038A1 (en) | Method for preparing polypeptide variants | |
| JP4531868B2 (ja) | １を超える非リボソームｒｎａをコードしているドメインの染色体ゲノムに組込まれた少なくとも２コピーの所望遺伝子を含む、特にクルイベロミセスによる酵母細胞 | |
| MXPA00006122A (en) | Expression cloning in filamentous fungi |