CA2313445C - Expression cloning in filamentous fungi - Google Patents
Expression cloning in filamentous fungi Download PDFInfo
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- CA2313445C CA2313445C CA2313445A CA2313445A CA2313445C CA 2313445 C CA2313445 C CA 2313445C CA 2313445 A CA2313445 A CA 2313445A CA 2313445 A CA2313445 A CA 2313445A CA 2313445 C CA2313445 C CA 2313445C
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
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- Bioinformatics & Cheminformatics (AREA)
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- Bioinformatics & Computational Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP97204079.4 | 1997-12-22 | ||
| EP97204079 | 1997-12-22 | ||
| PCT/EP1998/008577 WO1999032617A2 (en) | 1997-12-22 | 1998-12-22 | Expression cloning in filamentous fungi |
Publications (2)
| Publication Number | Publication Date |
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| CA2313445A1 CA2313445A1 (en) | 1999-07-01 |
| CA2313445C true CA2313445C (en) | 2012-03-20 |
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|---|---|---|---|
| CA2313445A Expired - Fee Related CA2313445C (en) | 1997-12-22 | 1998-12-22 | Expression cloning in filamentous fungi |
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|---|---|
| US (1) | US20020155536A1 (cg-RX-API-DMAC7.html) |
| EP (2) | EP1854890A1 (cg-RX-API-DMAC7.html) |
| JP (3) | JP4410413B2 (cg-RX-API-DMAC7.html) |
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| EA014853B1 (ru) * | 2004-10-15 | 2011-02-28 | ДСМ АйПи АССЕТС Б.В. | Амидаза из aspergillus niger и применение амидазы для получения пищевого продукта с пониженным содержанием акриламида |
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| WO2008098933A1 (en) | 2007-02-15 | 2008-08-21 | Dsm Ip Assets B.V. | A recombinant host cell for the production of a compound of interest |
| PL2113027T3 (pl) | 2007-02-20 | 2014-11-28 | Dsm Ip Assets Bv | Nowa sialidaza |
| EP2197893B1 (en) | 2007-09-07 | 2013-07-24 | Dyadic International, Inc. | Novel fungal enzymes |
| US8703463B2 (en) | 2009-03-10 | 2014-04-22 | Dsm Ip Assets B.V. | Pregastric esterase and derivatives thereof |
| EP2406386B1 (en) | 2009-03-10 | 2019-01-23 | DSM IP Assets B.V. | Method for improving the yield of a polypeptide |
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| US8734782B2 (en) | 2009-07-22 | 2014-05-27 | Dsm Ip Assets B.V. | Host cell for the production of a compound of interest |
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| EA201201125A1 (ru) | 2010-02-11 | 2013-03-29 | ДСМ АйПи АССЕТС Б.В. | Клетка-хозяин, способная продуцировать ферменты, пригодные для деградации лигноцеллюлозного материала |
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| CN104508114A (zh) | 2012-06-19 | 2015-04-08 | 帝斯曼知识产权资产管理有限公司 | 用于在细胞中表达基因的启动子 |
| DK3327112T3 (en) | 2012-07-19 | 2020-05-04 | Dsm Ip Assets Bv | Agse-deficient stamme |
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| ES2706742T3 (es) | 2013-12-02 | 2019-04-01 | Dsm Ip Assets Bv | Proteína estructuradora del hielo |
| ES2773889T3 (es) * | 2014-01-31 | 2020-07-15 | Api Corp | Hidroxilasa en la posición 4 de ácido pipecolínico y método para producir 4-hidroxiaminoácido usando la misma |
| WO2015177171A1 (en) | 2014-05-19 | 2015-11-26 | Dsm Ip Assets B.V. | Proline-specific endoprotease |
| WO2015177153A1 (en) | 2014-05-19 | 2015-11-26 | Dsm Ip Assets B.V. | Proline-specific endoprotease |
| WO2015177152A1 (en) | 2014-05-19 | 2015-11-26 | Dsm Ip Assets B.V. | Proline-specific endoprotease |
| EP3242950B1 (en) | 2015-01-06 | 2021-10-06 | DSM IP Assets B.V. | A crispr-cas system for a filamentous fungal host cell |
| AR104205A1 (es) | 2015-04-09 | 2017-07-05 | Dsm Ip Assets Bv | Fosfolipasa c |
| US20180160695A1 (en) | 2015-06-02 | 2018-06-14 | Dsm Ip Assets B.V. | Use of ice structuring protein afp19 expressed in filamentous fungal strains for preparing food |
| EP4361240A3 (en) | 2015-11-17 | 2024-07-31 | DSM IP Assets B.V. | Preparation of a stable beer |
| US10913938B2 (en) | 2016-07-29 | 2021-02-09 | Dsm Ip Assets B.V. | Polypeptides having cellulolytic enhancing activity and uses thereof |
| SI3296401T1 (sl) * | 2016-09-20 | 2020-07-31 | Clariant International Ltd | Metoda za selektivno vir ogljika-neodvisno ekspresijo protein-kodirajočih sekvenc v celici filamentozne glive |
| WO2017216392A1 (en) | 2016-09-23 | 2017-12-21 | Dsm Ip Assets B.V. | A guide-rna expression system for a host cell |
| EP3559221A1 (en) | 2016-12-21 | 2019-10-30 | DSM IP Assets B.V. | Lipolytic enzyme variants |
| US10918113B2 (en) | 2016-12-21 | 2021-02-16 | Dsm Ip Assets B.V. | Lipolytic enzyme variants |
| WO2018114938A1 (en) | 2016-12-21 | 2018-06-28 | Dsm Ip Assets B.V. | Lipolytic enzyme variants |
| WO2018114912A1 (en) | 2016-12-21 | 2018-06-28 | Dsm Ip Assets B.V. | Lipolytic enzyme variants |
| CN110431224A (zh) | 2017-03-13 | 2019-11-08 | 帝斯曼知识产权资产管理有限公司 | 锌双核簇转录调控因子缺陷型菌株 |
| CN110462044A (zh) | 2017-04-06 | 2019-11-15 | 帝斯曼知识产权资产管理有限公司 | 自引导整合构建体(sgic) |
| WO2019215078A1 (en) | 2018-05-07 | 2019-11-14 | Dsm Ip Assets B.V. | Process for enzymatic oil degumming |
| MX2020014191A (es) | 2018-06-19 | 2021-03-09 | Dsm Ip Assets Bv | Variantes de enzimas lipoliticas. |
| US20220389458A1 (en) | 2019-11-04 | 2022-12-08 | Dsm Ip Assets B.V. | Low volume transfection |
| JP2021093966A (ja) * | 2019-12-18 | 2021-06-24 | 本田技研工業株式会社 | 糸状菌への遺伝子導入方法及びその利用 |
| CA3225417A1 (en) | 2021-06-24 | 2022-12-29 | Fonterra Co-Operative Group Limited | Recombinant proteins |
| JP2025520009A (ja) | 2022-05-16 | 2025-07-01 | ディーエスエム アイピー アセッツ ビー．ブイ． | 脂肪分解酵素変異体 |
| WO2025012325A1 (en) | 2023-07-11 | 2025-01-16 | Dsm Ip Assets B.V. | Protein arginine deiminase |
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| DK122686D0 (da) * | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
| WO1990015860A1 (en) * | 1989-06-16 | 1990-12-27 | Genencor, Inc. | Dna sequences, vectors, and fusion polypeptides to increase secretion of desired polypeptides from filamentous fungi |
| CA2063592C (en) * | 1989-07-07 | 2003-01-21 | Marco L. F. Giuseppin | Process for preparing a protein by a fungus transformed by multicopy integration of an expression vector |
| JPH0595787A (ja) * | 1991-10-04 | 1993-04-20 | Jozo Shigen Kenkyusho:Kk | ホスホグリセリン酸キナ−ゼ遺伝子プロモ−タ−、 同タ−ミネ−タ−または／及びこれらからなる 遺伝子発現用ユニツト、並びに、ホスホグリセリ ン酸キナ−ゼのゲノム遺伝子及びｃＤＮＡ遺伝子 |
| MX9206979A (es) * | 1991-12-04 | 1993-07-01 | Novo Nordisk As | Metodo de clonacion de proteinas en levaduras |
| JPH05268964A (ja) * | 1992-03-30 | 1993-10-19 | Meiji Milk Prod Co Ltd | 糸状菌プロモーター |
| ES2198410T3 (es) * | 1993-07-23 | 2004-02-01 | Dsm Ip Assets B.V. | Cepas recombinantes de hongos filamentosos libres de gen marcador de seleccion: un metodo para obtener dichas cepas y su utilizacion. |
| JP3638657B2 (ja) * | 1995-03-29 | 2005-04-13 | 株式会社資生堂 | Ｄｎａ修復酵素 |
| EP0822990A4 (en) * | 1995-04-24 | 2002-07-03 | Chromaxome Corp | METHODS OF GENERATING AND SCREENING NEW METABOLIC PATHWAYS |
| JP3343567B2 (ja) * | 1995-06-29 | 2002-11-11 | 大関株式会社 | 糸状菌のエンハンサーｄｎａ塩基配列およびその用途 |
| CN101070537A (zh) * | 1996-01-10 | 2007-11-14 | 诺沃奇梅兹有限公司 | 用于在细胞中体内产生突变体文库方法 |
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1998
- 1998-12-22 ES ES98966868T patent/ES2287989T3/es not_active Expired - Lifetime
- 1998-12-22 EP EP07112086A patent/EP1854890A1/en not_active Withdrawn
- 1998-12-22 CA CA2313445A patent/CA2313445C/en not_active Expired - Fee Related
- 1998-12-22 AU AU25151/99A patent/AU736111C/en not_active Ceased
- 1998-12-22 DK DK98966868T patent/DK1042461T3/da active
- 1998-12-22 DE DE69838106T patent/DE69838106T2/de not_active Expired - Lifetime
- 1998-12-22 AT AT98966868T patent/ATE367439T1/de active
- 1998-12-22 JP JP2000525536A patent/JP4410413B2/ja not_active Expired - Fee Related
- 1998-12-22 WO PCT/EP1998/008577 patent/WO1999032617A2/en not_active Ceased
- 1998-12-22 CN CN98812563.3A patent/CN1198924C/zh not_active Expired - Lifetime
- 1998-12-22 BR BR9814404-9A patent/BR9814404A/pt not_active Application Discontinuation
- 1998-12-22 EP EP98966868A patent/EP1042461B1/en not_active Revoked
- 1998-12-22 PL PL98341760A patent/PL341760A1/xx unknown
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2001
- 2001-11-05 US US09/993,164 patent/US20020155536A1/en active Pending
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2005
- 2005-05-25 JP JP2005152703A patent/JP2005261438A/ja active Pending
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Also Published As
| Publication number | Publication date |
|---|---|
| DE69838106T2 (de) | 2008-04-03 |
| CA2313445A1 (en) | 1999-07-01 |
| EP1042461B1 (en) | 2007-07-18 |
| EP1042461A2 (en) | 2000-10-11 |
| BR9814404A (pt) | 2000-10-10 |
| US20020155536A1 (en) | 2002-10-24 |
| DK1042461T3 (da) | 2007-09-24 |
| JP4410413B2 (ja) | 2010-02-03 |
| AU736111B2 (en) | 2001-07-26 |
| PL341760A1 (en) | 2001-05-07 |
| ATE367439T1 (de) | 2007-08-15 |
| WO1999032617A2 (en) | 1999-07-01 |
| WO1999032617A3 (en) | 1999-09-10 |
| ES2287989T3 (es) | 2007-12-16 |
| JP2002509698A (ja) | 2002-04-02 |
| AU2515199A (en) | 1999-07-12 |
| CN1198924C (zh) | 2005-04-27 |
| JP2010046080A (ja) | 2010-03-04 |
| EP1854890A1 (en) | 2007-11-14 |
| CN1283227A (zh) | 2001-02-07 |
| JP2005261438A (ja) | 2005-09-29 |
| AU736111C (en) | 2004-02-19 |
| DE69838106D1 (de) | 2007-08-30 |
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