CN1198924C - 在丝状真菌中表达克隆 - Google Patents
在丝状真菌中表达克隆 Download PDFInfo
- Publication number
- CN1198924C CN1198924C CN98812563.3A CN98812563A CN1198924C CN 1198924 C CN1198924 C CN 1198924C CN 98812563 A CN98812563 A CN 98812563A CN 1198924 C CN1198924 C CN 1198924C
- Authority
- CN
- China
- Prior art keywords
- cdna
- dna
- expression
- gene
- vector
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Mycology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Mushroom Cultivation (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP97204079.4 | 1997-12-22 | ||
| EP97204079 | 1997-12-22 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1283227A CN1283227A (zh) | 2001-02-07 |
| CN1198924C true CN1198924C (zh) | 2005-04-27 |
Family
ID=8229117
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN98812563.3A Expired - Lifetime CN1198924C (zh) | 1997-12-22 | 1998-12-22 | 在丝状真菌中表达克隆 |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US20020155536A1 (cg-RX-API-DMAC7.html) |
| EP (2) | EP1854890A1 (cg-RX-API-DMAC7.html) |
| JP (3) | JP4410413B2 (cg-RX-API-DMAC7.html) |
| CN (1) | CN1198924C (cg-RX-API-DMAC7.html) |
| AT (1) | ATE367439T1 (cg-RX-API-DMAC7.html) |
| AU (1) | AU736111C (cg-RX-API-DMAC7.html) |
| BR (1) | BR9814404A (cg-RX-API-DMAC7.html) |
| CA (1) | CA2313445C (cg-RX-API-DMAC7.html) |
| DE (1) | DE69838106T2 (cg-RX-API-DMAC7.html) |
| DK (1) | DK1042461T3 (cg-RX-API-DMAC7.html) |
| ES (1) | ES2287989T3 (cg-RX-API-DMAC7.html) |
| PL (1) | PL341760A1 (cg-RX-API-DMAC7.html) |
| WO (1) | WO1999032617A2 (cg-RX-API-DMAC7.html) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102286483B (zh) * | 2004-04-16 | 2014-06-04 | 中化帝斯曼制药有限公司荷兰公司 | 用于在真菌细胞中表达基因的真菌启动子 |
Families Citing this family (65)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7883872B2 (en) | 1996-10-10 | 2011-02-08 | Dyadic International (Usa), Inc. | Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose |
| ATE376057T1 (de) | 1999-10-01 | 2007-11-15 | Novozymes As | Enzymgranulat |
| KR20020026456A (ko) | 2000-04-13 | 2002-04-10 | 에말파브 마크 아론 | 사상균에서 발현된 dna 라이브러리의 고산출량 스크리닝 |
| DE60138947D1 (de) * | 2000-04-13 | 2009-07-23 | Dyadic Internat Usa Inc | Durchmusterung von bibliotheken exprimierter dna in filamentösen pilzen mit hoher durchsatzrate |
| AU7779800A (en) | 2000-09-21 | 2002-04-02 | Dsm Nv | Talaromyces xylanases |
| MXPA03005128A (es) | 2000-12-07 | 2004-12-06 | Dsm Ip Assets Bv | Hidrolizados proteicos enriquecidos en peptidos que tienen un residuo carboxiterminal de prolina. |
| WO2003054186A1 (en) | 2001-12-21 | 2003-07-03 | Dsm Ip Assets B.V. | New rennets |
| TWI328612B (en) | 2002-07-25 | 2010-08-11 | Dsm Ip Assets Bv | Genes and their encoded polypeptides involved in the biosynthetic pathway of vitamin b12, vectors and host cells comprising the genes, and process for producing vitamin b12 |
| WO2004078919A2 (en) | 2003-02-27 | 2004-09-16 | Midwest Research Institute | Superactive cellulase formulation using cellobiohydrolase-1 from penicillium funiculosum |
| US20050059024A1 (en) | 2003-07-25 | 2005-03-17 | Ambion, Inc. | Methods and compositions for isolating small RNA molecules |
| CA2565888C (en) | 2004-05-27 | 2014-10-14 | Novozymes, Inc. | Methods for transforming and expression screening of filamentous fungal cells with a dna library |
| EA014853B1 (ru) * | 2004-10-15 | 2011-02-28 | ДСМ АйПи АССЕТС Б.В. | Амидаза из aspergillus niger и применение амидазы для получения пищевого продукта с пониженным содержанием акриламида |
| CN101107354B (zh) | 2005-01-24 | 2012-05-30 | 帝斯曼知识产权资产管理有限公司 | 用于在有丝真菌细胞中生产感兴趣化合物的方法 |
| EP1859036A2 (en) * | 2005-03-18 | 2007-11-28 | DSMIP Assets B.V. | Method of expression cloning in a host cell |
| EP1954812B1 (en) | 2005-11-29 | 2012-11-21 | DSM IP Assets B.V. | Dna binding site of a transcriptional activator useful in gene expression |
| WO2007063133A2 (en) | 2005-12-01 | 2007-06-07 | Dsm Ip Assets B.V. | Genes useful for the industrial production of citric acid |
| CA2657975A1 (en) | 2006-06-29 | 2008-01-03 | Dsm Ip Assets B.V. | A method for achieving improved polypeptide expression |
| WO2008053018A2 (en) | 2006-11-02 | 2008-05-08 | Dsm Ip Assets B.V. | Improved production of secreted proteins by filamentous fungi |
| WO2008073914A2 (en) | 2006-12-10 | 2008-06-19 | Dyadic International Inc. | Expression and high-throughput screening of complex expressed dna libraries in filamentous fungi |
| WO2008098933A1 (en) | 2007-02-15 | 2008-08-21 | Dsm Ip Assets B.V. | A recombinant host cell for the production of a compound of interest |
| PL2113027T3 (pl) | 2007-02-20 | 2014-11-28 | Dsm Ip Assets Bv | Nowa sialidaza |
| EP2197893B1 (en) | 2007-09-07 | 2013-07-24 | Dyadic International, Inc. | Novel fungal enzymes |
| US8703463B2 (en) | 2009-03-10 | 2014-04-22 | Dsm Ip Assets B.V. | Pregastric esterase and derivatives thereof |
| EP2406386B1 (en) | 2009-03-10 | 2019-01-23 | DSM IP Assets B.V. | Method for improving the yield of a polypeptide |
| WO2010121933A1 (en) | 2009-04-22 | 2010-10-28 | Dsm Ip Assets B.V. | Process for the production of a recombinant polypeptide of interest |
| US8734782B2 (en) | 2009-07-22 | 2014-05-27 | Dsm Ip Assets B.V. | Host cell for the production of a compound of interest |
| CA2779796A1 (en) | 2009-11-04 | 2011-05-12 | Dsm Ip Assets B.V. | Talaromyces transformants |
| CN103347997A (zh) | 2010-02-04 | 2013-10-09 | 帝斯曼知识产权资产管理有限公司 | 制备具有性周期的丝状真菌菌株的方法和制备有性杂交丝状真菌菌株的方法 |
| EA201201125A1 (ru) | 2010-02-11 | 2013-03-29 | ДСМ АйПи АССЕТС Б.В. | Клетка-хозяин, способная продуцировать ферменты, пригодные для деградации лигноцеллюлозного материала |
| AU2011273351A1 (en) | 2010-07-01 | 2013-01-17 | Dsm Ip Assets B.V. | A method for the production of a compound of interest |
| WO2012007446A1 (en) | 2010-07-13 | 2012-01-19 | Dsm Ip Assets B.V. | Use of a mutarotase in the production of dried powders |
| WO2012007445A1 (en) | 2010-07-13 | 2012-01-19 | Dsm Ip Assets B.V. | Mutarotase in crystallization |
| CN104508114A (zh) | 2012-06-19 | 2015-04-08 | 帝斯曼知识产权资产管理有限公司 | 用于在细胞中表达基因的启动子 |
| DK3327112T3 (en) | 2012-07-19 | 2020-05-04 | Dsm Ip Assets Bv | Agse-deficient stamme |
| KR102134498B1 (ko) | 2013-02-04 | 2020-07-16 | 디에스엠 아이피 어셋츠 비.브이. | 탄수화물 분해 폴리펩티드 및 이의 용도 |
| WO2014142647A1 (en) | 2013-03-14 | 2014-09-18 | Wageningen Universiteit | Fungals strains with improved citric acid and itaconic acid production |
| WO2014202624A2 (en) | 2013-06-19 | 2014-12-24 | Dsm Ip Assets B.V. | Rasamsonia gene and use thereof |
| WO2014202622A2 (en) | 2013-06-19 | 2014-12-24 | Dsm Ip Assets B.V. | Rasamsonia gene and use thereof |
| WO2014202620A2 (en) | 2013-06-19 | 2014-12-24 | Dsm Ip Assets B.V. | Rasamsonia gene and use thereof |
| WO2014202621A1 (en) | 2013-06-20 | 2014-12-24 | Dsm Ip Assets B.V. | Carbohydrate degrading polypeptide and uses thereof |
| ES2706742T3 (es) | 2013-12-02 | 2019-04-01 | Dsm Ip Assets Bv | Proteína estructuradora del hielo |
| ES2773889T3 (es) * | 2014-01-31 | 2020-07-15 | Api Corp | Hidroxilasa en la posición 4 de ácido pipecolínico y método para producir 4-hidroxiaminoácido usando la misma |
| WO2015177171A1 (en) | 2014-05-19 | 2015-11-26 | Dsm Ip Assets B.V. | Proline-specific endoprotease |
| WO2015177153A1 (en) | 2014-05-19 | 2015-11-26 | Dsm Ip Assets B.V. | Proline-specific endoprotease |
| WO2015177152A1 (en) | 2014-05-19 | 2015-11-26 | Dsm Ip Assets B.V. | Proline-specific endoprotease |
| EP3242950B1 (en) | 2015-01-06 | 2021-10-06 | DSM IP Assets B.V. | A crispr-cas system for a filamentous fungal host cell |
| AR104205A1 (es) | 2015-04-09 | 2017-07-05 | Dsm Ip Assets Bv | Fosfolipasa c |
| US20180160695A1 (en) | 2015-06-02 | 2018-06-14 | Dsm Ip Assets B.V. | Use of ice structuring protein afp19 expressed in filamentous fungal strains for preparing food |
| EP4361240A3 (en) | 2015-11-17 | 2024-07-31 | DSM IP Assets B.V. | Preparation of a stable beer |
| US10913938B2 (en) | 2016-07-29 | 2021-02-09 | Dsm Ip Assets B.V. | Polypeptides having cellulolytic enhancing activity and uses thereof |
| SI3296401T1 (sl) * | 2016-09-20 | 2020-07-31 | Clariant International Ltd | Metoda za selektivno vir ogljika-neodvisno ekspresijo protein-kodirajočih sekvenc v celici filamentozne glive |
| WO2017216392A1 (en) | 2016-09-23 | 2017-12-21 | Dsm Ip Assets B.V. | A guide-rna expression system for a host cell |
| EP3559221A1 (en) | 2016-12-21 | 2019-10-30 | DSM IP Assets B.V. | Lipolytic enzyme variants |
| US10918113B2 (en) | 2016-12-21 | 2021-02-16 | Dsm Ip Assets B.V. | Lipolytic enzyme variants |
| WO2018114938A1 (en) | 2016-12-21 | 2018-06-28 | Dsm Ip Assets B.V. | Lipolytic enzyme variants |
| WO2018114912A1 (en) | 2016-12-21 | 2018-06-28 | Dsm Ip Assets B.V. | Lipolytic enzyme variants |
| CN110431224A (zh) | 2017-03-13 | 2019-11-08 | 帝斯曼知识产权资产管理有限公司 | 锌双核簇转录调控因子缺陷型菌株 |
| CN110462044A (zh) | 2017-04-06 | 2019-11-15 | 帝斯曼知识产权资产管理有限公司 | 自引导整合构建体(sgic) |
| WO2019215078A1 (en) | 2018-05-07 | 2019-11-14 | Dsm Ip Assets B.V. | Process for enzymatic oil degumming |
| MX2020014191A (es) | 2018-06-19 | 2021-03-09 | Dsm Ip Assets Bv | Variantes de enzimas lipoliticas. |
| US20220389458A1 (en) | 2019-11-04 | 2022-12-08 | Dsm Ip Assets B.V. | Low volume transfection |
| JP2021093966A (ja) * | 2019-12-18 | 2021-06-24 | 本田技研工業株式会社 | 糸状菌への遺伝子導入方法及びその利用 |
| CA3225417A1 (en) | 2021-06-24 | 2022-12-29 | Fonterra Co-Operative Group Limited | Recombinant proteins |
| JP2025520009A (ja) | 2022-05-16 | 2025-07-01 | ディーエスエム アイピー アセッツ ビー.ブイ. | 脂肪分解酵素変異体 |
| WO2025012325A1 (en) | 2023-07-11 | 2025-01-16 | Dsm Ip Assets B.V. | Protein arginine deiminase |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK122686D0 (da) * | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
| WO1990015860A1 (en) * | 1989-06-16 | 1990-12-27 | Genencor, Inc. | Dna sequences, vectors, and fusion polypeptides to increase secretion of desired polypeptides from filamentous fungi |
| CA2063592C (en) * | 1989-07-07 | 2003-01-21 | Marco L. F. Giuseppin | Process for preparing a protein by a fungus transformed by multicopy integration of an expression vector |
| JPH0595787A (ja) * | 1991-10-04 | 1993-04-20 | Jozo Shigen Kenkyusho:Kk | ホスホグリセリン酸キナ−ゼ遺伝子プロモ−タ−、 同タ−ミネ−タ−または/及びこれらからなる 遺伝子発現用ユニツト、並びに、ホスホグリセリ ン酸キナ−ゼのゲノム遺伝子及びcDNA遺伝子 |
| MX9206979A (es) * | 1991-12-04 | 1993-07-01 | Novo Nordisk As | Metodo de clonacion de proteinas en levaduras |
| JPH05268964A (ja) * | 1992-03-30 | 1993-10-19 | Meiji Milk Prod Co Ltd | 糸状菌プロモーター |
| ES2198410T3 (es) * | 1993-07-23 | 2004-02-01 | Dsm Ip Assets B.V. | Cepas recombinantes de hongos filamentosos libres de gen marcador de seleccion: un metodo para obtener dichas cepas y su utilizacion. |
| JP3638657B2 (ja) * | 1995-03-29 | 2005-04-13 | 株式会社資生堂 | Dna修復酵素 |
| EP0822990A4 (en) * | 1995-04-24 | 2002-07-03 | Chromaxome Corp | METHODS OF GENERATING AND SCREENING NEW METABOLIC PATHWAYS |
| JP3343567B2 (ja) * | 1995-06-29 | 2002-11-11 | 大関株式会社 | 糸状菌のエンハンサーdna塩基配列およびその用途 |
| CN101070537A (zh) * | 1996-01-10 | 2007-11-14 | 诺沃奇梅兹有限公司 | 用于在细胞中体内产生突变体文库方法 |
-
1998
- 1998-12-22 ES ES98966868T patent/ES2287989T3/es not_active Expired - Lifetime
- 1998-12-22 EP EP07112086A patent/EP1854890A1/en not_active Withdrawn
- 1998-12-22 CA CA2313445A patent/CA2313445C/en not_active Expired - Fee Related
- 1998-12-22 AU AU25151/99A patent/AU736111C/en not_active Ceased
- 1998-12-22 DK DK98966868T patent/DK1042461T3/da active
- 1998-12-22 DE DE69838106T patent/DE69838106T2/de not_active Expired - Lifetime
- 1998-12-22 AT AT98966868T patent/ATE367439T1/de active
- 1998-12-22 JP JP2000525536A patent/JP4410413B2/ja not_active Expired - Fee Related
- 1998-12-22 WO PCT/EP1998/008577 patent/WO1999032617A2/en not_active Ceased
- 1998-12-22 CN CN98812563.3A patent/CN1198924C/zh not_active Expired - Lifetime
- 1998-12-22 BR BR9814404-9A patent/BR9814404A/pt not_active Application Discontinuation
- 1998-12-22 EP EP98966868A patent/EP1042461B1/en not_active Revoked
- 1998-12-22 PL PL98341760A patent/PL341760A1/xx unknown
-
2001
- 2001-11-05 US US09/993,164 patent/US20020155536A1/en active Pending
-
2005
- 2005-05-25 JP JP2005152703A patent/JP2005261438A/ja active Pending
-
2009
- 2009-10-20 JP JP2009241780A patent/JP2010046080A/ja active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102286483B (zh) * | 2004-04-16 | 2014-06-04 | 中化帝斯曼制药有限公司荷兰公司 | 用于在真菌细胞中表达基因的真菌启动子 |
Also Published As
| Publication number | Publication date |
|---|---|
| DE69838106T2 (de) | 2008-04-03 |
| CA2313445A1 (en) | 1999-07-01 |
| EP1042461B1 (en) | 2007-07-18 |
| EP1042461A2 (en) | 2000-10-11 |
| BR9814404A (pt) | 2000-10-10 |
| US20020155536A1 (en) | 2002-10-24 |
| DK1042461T3 (da) | 2007-09-24 |
| JP4410413B2 (ja) | 2010-02-03 |
| AU736111B2 (en) | 2001-07-26 |
| PL341760A1 (en) | 2001-05-07 |
| ATE367439T1 (de) | 2007-08-15 |
| WO1999032617A2 (en) | 1999-07-01 |
| WO1999032617A3 (en) | 1999-09-10 |
| ES2287989T3 (es) | 2007-12-16 |
| JP2002509698A (ja) | 2002-04-02 |
| AU2515199A (en) | 1999-07-12 |
| JP2010046080A (ja) | 2010-03-04 |
| EP1854890A1 (en) | 2007-11-14 |
| CN1283227A (zh) | 2001-02-07 |
| CA2313445C (en) | 2012-03-20 |
| JP2005261438A (ja) | 2005-09-29 |
| AU736111C (en) | 2004-02-19 |
| DE69838106D1 (de) | 2007-08-30 |
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