ES2225999T3 - Aplicacion de esfuerzo por flujo de cizallamiento a condrocitos. - Google Patents
Aplicacion de esfuerzo por flujo de cizallamiento a condrocitos.Info
- Publication number
- ES2225999T3 ES2225999T3 ES97948366T ES97948366T ES2225999T3 ES 2225999 T3 ES2225999 T3 ES 2225999T3 ES 97948366 T ES97948366 T ES 97948366T ES 97948366 T ES97948366 T ES 97948366T ES 2225999 T3 ES2225999 T3 ES 2225999T3
- Authority
- ES
- Spain
- Prior art keywords
- cells
- substrate
- shear flow
- effort
- chondrocytes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 210000004027 cell Anatomy 0.000 claims abstract description 99
- 210000001612 chondrocyte Anatomy 0.000 claims abstract description 84
- 230000012010 growth Effects 0.000 claims abstract description 43
- 210000000845 cartilage Anatomy 0.000 claims abstract description 41
- 239000000758 substrate Substances 0.000 claims abstract description 40
- 239000007788 liquid Substances 0.000 claims abstract description 31
- 239000001963 growth medium Substances 0.000 claims abstract description 28
- 210000000130 stem cell Anatomy 0.000 claims abstract description 25
- 239000002609 medium Substances 0.000 claims abstract description 23
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- 238000009630 liquid culture Methods 0.000 claims abstract description 13
- 210000004962 mammalian cell Anatomy 0.000 claims abstract description 8
- 102000000503 Collagen Type II Human genes 0.000 claims abstract description 7
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- 238000000034 method Methods 0.000 claims description 49
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- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 1
- 229920001917 Ficoll Polymers 0.000 description 1
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 1
- 229930182566 Gentamicin Natural products 0.000 description 1
- PMMYEEVYMWASQN-DMTCNVIQSA-N Hydroxyproline Chemical compound O[C@H]1CN[C@H](C(O)=O)C1 PMMYEEVYMWASQN-DMTCNVIQSA-N 0.000 description 1
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
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- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 102000016611 Proteoglycans Human genes 0.000 description 1
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- 210000001185 bone marrow Anatomy 0.000 description 1
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- 239000002729 catgut Substances 0.000 description 1
- 238000012832 cell culture technique Methods 0.000 description 1
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- 238000009792 diffusion process Methods 0.000 description 1
- IPZJQDSFZGZEOY-UHFFFAOYSA-N dimethylmethylene Chemical group C[C]C IPZJQDSFZGZEOY-UHFFFAOYSA-N 0.000 description 1
- PMMYEEVYMWASQN-UHFFFAOYSA-N dl-hydroxyproline Natural products OC1C[NH2+]C(C([O-])=O)C1 PMMYEEVYMWASQN-UHFFFAOYSA-N 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 210000001162 elastic cartilage Anatomy 0.000 description 1
- 238000010894 electron beam technology Methods 0.000 description 1
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- 230000002255 enzymatic effect Effects 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 239000012091 fetal bovine serum Substances 0.000 description 1
- 210000000968 fibrocartilage Anatomy 0.000 description 1
- 238000005188 flotation Methods 0.000 description 1
- 229960002518 gentamicin Drugs 0.000 description 1
- 210000004276 hyalin Anatomy 0.000 description 1
- 229960002591 hydroxyproline Drugs 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 235000015110 jellies Nutrition 0.000 description 1
- 239000008274 jelly Substances 0.000 description 1
- 210000003127 knee Anatomy 0.000 description 1
- 210000000629 knee joint Anatomy 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 238000011587 new zealand white rabbit Methods 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
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- 210000003460 periosteum Anatomy 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
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- 229920000058 polyacrylate Polymers 0.000 description 1
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- 229920000728 polyester Polymers 0.000 description 1
- 239000005020 polyethylene terephthalate Substances 0.000 description 1
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- 229920001343 polytetrafluoroethylene Polymers 0.000 description 1
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- OARRHUQTFTUEOS-UHFFFAOYSA-N safranin Chemical compound [Cl-].C=12C=C(N)C(C)=CC2=NC2=CC(C)=C(N)C=C2[N+]=1C1=CC=CC=C1 OARRHUQTFTUEOS-UHFFFAOYSA-N 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 238000003307 slaughter Methods 0.000 description 1
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- FGMPLJWBKKVCDB-UHFFFAOYSA-N trans-L-hydroxy-proline Natural products ON1CCCC1C(O)=O FGMPLJWBKKVCDB-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0655—Chondrocytes; Cartilage
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/14—Scaffolds; Matrices
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Cell Biology (AREA)
- Rheumatology (AREA)
- Immunology (AREA)
- Mechanical Engineering (AREA)
- Molecular Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Excavating Of Shafts Or Tunnels (AREA)
- Lubricants (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Materials For Medical Uses (AREA)
- Curing Cements, Concrete, And Artificial Stone (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/753,104 US5928945A (en) | 1996-11-20 | 1996-11-20 | Application of shear flow stress to chondrocytes or chondrocyte stem cells to produce cartilage |
| US753104 | 1996-11-20 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2225999T3 true ES2225999T3 (es) | 2005-03-16 |
Family
ID=25029173
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES97948366T Expired - Lifetime ES2225999T3 (es) | 1996-11-20 | 1997-11-18 | Aplicacion de esfuerzo por flujo de cizallamiento a condrocitos. |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US5928945A (enExample) |
| EP (1) | EP1019489B1 (enExample) |
| JP (1) | JP4014645B2 (enExample) |
| KR (1) | KR20000057136A (enExample) |
| AT (1) | ATE273382T1 (enExample) |
| AU (1) | AU744686B2 (enExample) |
| CA (1) | CA2272547A1 (enExample) |
| DE (1) | DE69730251T2 (enExample) |
| ES (1) | ES2225999T3 (enExample) |
| NZ (1) | NZ335991A (enExample) |
| WO (1) | WO1998022573A1 (enExample) |
Families Citing this family (122)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7468075B2 (en) | 2001-05-25 | 2008-12-23 | Conformis, Inc. | Methods and compositions for articular repair |
| US9603711B2 (en) | 2001-05-25 | 2017-03-28 | Conformis, Inc. | Patient-adapted and improved articular implants, designs and related guide tools |
| US8882847B2 (en) | 2001-05-25 | 2014-11-11 | Conformis, Inc. | Patient selectable knee joint arthroplasty devices |
| US8771365B2 (en) | 2009-02-25 | 2014-07-08 | Conformis, Inc. | Patient-adapted and improved orthopedic implants, designs, and related tools |
| US8735773B2 (en) | 2007-02-14 | 2014-05-27 | Conformis, Inc. | Implant device and method for manufacture |
| US8480754B2 (en) | 2001-05-25 | 2013-07-09 | Conformis, Inc. | Patient-adapted and improved articular implants, designs and related guide tools |
| US8556983B2 (en) | 2001-05-25 | 2013-10-15 | Conformis, Inc. | Patient-adapted and improved orthopedic implants, designs and related tools |
| US8545569B2 (en) | 2001-05-25 | 2013-10-01 | Conformis, Inc. | Patient selectable knee arthroplasty devices |
| IL124722A0 (en) * | 1998-06-02 | 1999-01-26 | Oron Amir | Ischemia laser treatment |
| US8563232B2 (en) | 2000-09-12 | 2013-10-22 | Lifenet Health | Process for devitalizing soft-tissue engineered medical implants, and devitalized soft-tissue medical implants produced |
| US7063726B2 (en) * | 1998-06-30 | 2006-06-20 | Lifenet | Plasticized bone grafts and methods of making and using same |
| US20100030340A1 (en) * | 1998-06-30 | 2010-02-04 | Wolfinbarger Jr Lloyd | Plasticized Grafts and Methods of Making and Using Same |
| US6293970B1 (en) * | 1998-06-30 | 2001-09-25 | Lifenet | Plasticized bone and soft tissue grafts and methods of making and using same |
| US7239908B1 (en) | 1998-09-14 | 2007-07-03 | The Board Of Trustees Of The Leland Stanford Junior University | Assessing the condition of a joint and devising treatment |
| AU772012B2 (en) | 1998-09-14 | 2004-04-08 | Board Of Trustees Of The Leland Stanford Junior University | Assessing the condition of a joint and preventing damage |
| US6287340B1 (en) | 1999-05-14 | 2001-09-11 | Trustees Of Tufts College | Bioengineered anterior cruciate ligament |
| WO2000073417A1 (en) | 1999-05-27 | 2000-12-07 | The Research Foundation Of State University Of New York | In vitro cell culture device including cartilage and methods of using the same |
| US6652872B2 (en) | 1999-07-06 | 2003-11-25 | Ramat At Tel Aviv University Ltd. | Scaffold formed of tissue treated to eliminate cellular and cytosolic elements |
| US6632651B1 (en) | 1999-07-06 | 2003-10-14 | Ramot At Tel Aviv University Ltd. | Tissue maintenance system that applies rhythmic pulses of pressure |
| US7005274B1 (en) * | 1999-09-15 | 2006-02-28 | Migenix Corp. | Methods and compositions for diagnosing and treating arthritic disorders and regulating bone mass |
| US7585323B2 (en) * | 2000-05-05 | 2009-09-08 | Medlden, Llc | In vitro mechanical loading of musculoskeletal tissues |
| WO2002017983A1 (en) * | 2000-08-28 | 2002-03-07 | Osteogenesis Co., Ltd. | Medicinal compositions for forming tissue around bone or tooth, process for preparing the same, injections for forming tissue around bone or tooth and process for preparing the same |
| AU2001290888B8 (en) | 2000-09-14 | 2007-07-26 | The Board Of Trustees Of The Leland Stanford Junior University | Assessing the condition of a joint and devising treatment |
| ATE414310T1 (de) | 2000-09-14 | 2008-11-15 | Univ Leland Stanford Junior | Verfahren zur manipulation medizinischer bilder |
| AU2001296873A1 (en) | 2000-09-14 | 2002-03-26 | Leland Stanford Junior University | Technique for manipulating medical images |
| US7063942B2 (en) * | 2000-10-06 | 2006-06-20 | Victor Krstec | System and method to simulate hemodynamics |
| US6835390B1 (en) * | 2000-11-17 | 2004-12-28 | Jon Vein | Method for producing tissue engineered meat for consumption |
| DE10061704A1 (de) * | 2000-12-12 | 2002-06-20 | Hans Joerg Bauer | Verfahren und Vorrichtung zur Herstellung von biologischem Gewebe in einer Wachstumskammer |
| US7326564B2 (en) | 2001-02-20 | 2008-02-05 | St. Jude Medical, Inc. | Flow system for medical device evaluation and production |
| US20040151705A1 (en) | 2002-03-22 | 2004-08-05 | Shuichi Mizuno | Neo-cartilage constructs and a method for preparation thereof |
| WO2002076285A2 (en) | 2001-03-23 | 2002-10-03 | Histogenics Corporation | Composition and methods fro the production of biological tissues and tissue constructs |
| JP2002306157A (ja) * | 2001-04-17 | 2002-10-22 | Takagi Ind Co Ltd | 細胞・組織培養装置 |
| JP2002315566A (ja) | 2001-04-24 | 2002-10-29 | Takagi Ind Co Ltd | 細胞・組織培養装置 |
| DE60239674D1 (de) | 2001-05-25 | 2011-05-19 | Conformis Inc | Verfahren und zusammensetzungen zur reparatur der oberfläche von gelenken |
| DE10130512B4 (de) * | 2001-06-25 | 2007-08-16 | Bionethos Holding Gmbh | Vorrichtung zur Druckperfusion für das Züchten und/oder für das Behandeln von Zellen |
| US7303578B2 (en) | 2001-11-01 | 2007-12-04 | Photothera, Inc. | Device and method for providing phototherapy to the brain |
| US8308784B2 (en) | 2006-08-24 | 2012-11-13 | Jackson Streeter | Low level light therapy for enhancement of neurologic function of a patient affected by Parkinson's disease |
| US7534255B1 (en) * | 2003-01-24 | 2009-05-19 | Photothera, Inc | Low level light therapy for enhancement of neurologic function |
| US9993659B2 (en) * | 2001-11-01 | 2018-06-12 | Pthera, Llc | Low level light therapy for enhancement of neurologic function by altering axonal transport rate |
| US10683494B2 (en) | 2001-11-01 | 2020-06-16 | Pthera LLC | Enhanced stem cell therapy and stem cell production through the administration of low level light energy |
| US20030166274A1 (en) * | 2001-11-15 | 2003-09-04 | Hewitt Charles W. | Three-dimensional matrix for producing living tissue equivalents |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US5041138A (en) * | 1986-11-20 | 1991-08-20 | Massachusetts Institute Of Technology | Neomorphogenesis of cartilage in vivo from cell culture |
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-
1996
- 1996-11-20 US US08/753,104 patent/US5928945A/en not_active Expired - Lifetime
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1997
- 1997-11-18 JP JP52382798A patent/JP4014645B2/ja not_active Expired - Fee Related
- 1997-11-18 AT AT97948366T patent/ATE273382T1/de not_active IP Right Cessation
- 1997-11-18 WO PCT/US1997/021088 patent/WO1998022573A1/en not_active Ceased
- 1997-11-18 EP EP97948366A patent/EP1019489B1/en not_active Expired - Lifetime
- 1997-11-18 CA CA002272547A patent/CA2272547A1/en not_active Abandoned
- 1997-11-18 AU AU54451/98A patent/AU744686B2/en not_active Ceased
- 1997-11-18 NZ NZ335991A patent/NZ335991A/xx unknown
- 1997-11-18 DE DE69730251T patent/DE69730251T2/de not_active Expired - Lifetime
- 1997-11-18 KR KR1019990704403A patent/KR20000057136A/ko not_active Ceased
- 1997-11-18 ES ES97948366T patent/ES2225999T3/es not_active Expired - Lifetime
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| WO1998022573A1 (en) | 1998-05-28 |
| AU5445198A (en) | 1998-06-10 |
| EP1019489A4 (en) | 2002-08-28 |
| DE69730251T2 (de) | 2005-09-15 |
| CA2272547A1 (en) | 1998-05-28 |
| EP1019489B1 (en) | 2004-08-11 |
| US5928945A (en) | 1999-07-27 |
| KR20000057136A (ko) | 2000-09-15 |
| ATE273382T1 (de) | 2004-08-15 |
| JP4014645B2 (ja) | 2007-11-28 |
| DE69730251D1 (de) | 2004-09-16 |
| NZ335991A (en) | 2000-07-28 |
| AU744686B2 (en) | 2002-02-28 |
| JP2001504697A (ja) | 2001-04-10 |
| EP1019489A1 (en) | 2000-07-19 |
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