ES2194957T3 - Metodo para el analisis en serie de expresion genica. - Google Patents

Metodo para el analisis en serie de expresion genica.

Info

Publication number
ES2194957T3
ES2194957T3 ES96306634T ES96306634T ES2194957T3 ES 2194957 T3 ES2194957 T3 ES 2194957T3 ES 96306634 T ES96306634 T ES 96306634T ES 96306634 T ES96306634 T ES 96306634T ES 2194957 T3 ES2194957 T3 ES 2194957T3
Authority
ES
Spain
Prior art keywords
gene expression
expression analysis
sage
transcripts
novel
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96306634T
Other languages
English (en)
Inventor
Kenneth W Kinzler
Victor E Velculescu
Bert Vogelstein
Lin Zhang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Johns Hopkins University
School of Medicine of Johns Hopkins University
Original Assignee
Johns Hopkins University
School of Medicine of Johns Hopkins University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/527,154 external-priority patent/US5695937A/en
Application filed by Johns Hopkins University, School of Medicine of Johns Hopkins University filed Critical Johns Hopkins University
Application granted granted Critical
Publication of ES2194957T3 publication Critical patent/ES2194957T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809Methods for determination or identification of nucleic acids involving differential detection
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing

Abstract

SE PROPORCIONA UN METODO DE ANALISIS SECUENCIAL, SAGE, METODO RAPIDO PARA EL ANALISIS CUANTITATIVO Y CUALITATIVO DE TRANSCRITOS. SE AISLAN Y ANALIZAN MARCADORES DE SECUENCIA CORTOS Y DEFINIDOS, CORRESPONDIENTES A LOS GENES EXPRESADOS. LA SECUENCIACION DE MAS DE 1000 MARCADORES DEFINIDOS EN UN CORTO PERIODO DE TIEMPO (P.EJ., HORAS), MUESTRA UN PATRON DE EXPRESION GENICA CARACTERISTICO DE LA FUNCION DE UNA CELULA O TEJIDO. ADEMAS, SAGE ES UTIL COMO UNA HERRAMIENTA DE DESCUBRIMIENTO DE GENES, PARA LA IDENTIFICACION Y AISLAMIENTO DE NUEVOS MARCADORES DE SECUENCIA, CORRESPONDIENTES A NUEVOS TRANSCRITOS Y GENES.
ES96306634T 1995-09-12 1996-09-12 Metodo para el analisis en serie de expresion genica. Expired - Lifetime ES2194957T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/527,154 US5695937A (en) 1995-09-12 1995-09-12 Method for serial analysis of gene expression
US08/544,861 US5866330A (en) 1995-09-12 1995-10-18 Method for serial analysis of gene expression

Publications (1)

Publication Number Publication Date
ES2194957T3 true ES2194957T3 (es) 2003-12-01

Family

ID=27062344

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96306634T Expired - Lifetime ES2194957T3 (es) 1995-09-12 1996-09-12 Metodo para el analisis en serie de expresion genica.

Country Status (12)

Country Link
US (3) US5866330A (es)
EP (2) EP0761822B1 (es)
JP (3) JP3334806B2 (es)
AT (1) ATE239093T1 (es)
AU (2) AU7018896A (es)
CA (1) CA2185379A1 (es)
DE (2) DE69627768T2 (es)
DK (1) DK0761822T3 (es)
ES (1) ES2194957T3 (es)
GB (1) GB2305241B (es)
IE (1) IE80465B1 (es)
WO (1) WO1997010363A1 (es)

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Also Published As

Publication number Publication date
GB2305241A (en) 1997-04-02
ATE239093T1 (de) 2003-05-15
AU6561496A (en) 1997-03-20
US6383743B1 (en) 2002-05-07
US5866330A (en) 1999-02-02
EP0761822A3 (en) 1998-08-05
EP0761822B1 (en) 2003-05-02
WO1997010363A1 (en) 1997-03-20
DK0761822T3 (da) 2003-08-18
GB9619024D0 (en) 1996-10-23
JP3334806B2 (ja) 2002-10-15
EP0761822A2 (en) 1997-03-12
US6746845B2 (en) 2004-06-08
EP1231284A3 (en) 2003-02-26
IE80465B1 (en) 1998-08-12
JPH10511002A (ja) 1998-10-27
DE761822T1 (de) 2001-01-11
EP1231284A2 (en) 2002-08-14
CA2185379A1 (en) 1997-03-13
DE69627768D1 (de) 2003-06-05
AU7018896A (en) 1997-04-01
US20030049653A1 (en) 2003-03-13
DE69627768T2 (de) 2004-04-08
AU707846B2 (en) 1999-07-22
JP2001155035A (ja) 2001-06-08
JP2001145495A (ja) 2001-05-29
GB2305241B (en) 1999-11-10

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