ES2155854T3 - Procedimiento de produccion de proteinas de fusion que comprende fragmentos de scfv con la ayuda de un molde transformado. - Google Patents

Procedimiento de produccion de proteinas de fusion que comprende fragmentos de scfv con la ayuda de un molde transformado.

Info

Publication number
ES2155854T3
ES2155854T3 ES94920443T ES94920443T ES2155854T3 ES 2155854 T3 ES2155854 T3 ES 2155854T3 ES 94920443 T ES94920443 T ES 94920443T ES 94920443 T ES94920443 T ES 94920443T ES 2155854 T3 ES2155854 T3 ES 2155854T3
Authority
ES
Spain
Prior art keywords
gene
aspergillus
present
scfv
mould
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94920443T
Other languages
English (en)
Inventor
Leon Gerardus Joseph Frenken
Gorcom Robert F M Van
Johanna G M Hessing
Den Hondel Cornelis Antoni Van
Wouter Musters
Johannes Mariaa Verbakel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nederlandse Organisatie voor Toegepast Natuurwetenschappelijk Onderzoek TNO
Unilever NV
Original Assignee
Nederlandse Organisatie voor Toegepast Natuurwetenschappelijk Onderzoek TNO
Unilever NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nederlandse Organisatie voor Toegepast Natuurwetenschappelijk Onderzoek TNO, Unilever NV filed Critical Nederlandse Organisatie voor Toegepast Natuurwetenschappelijk Onderzoek TNO
Application granted granted Critical
Publication of ES2155854T3 publication Critical patent/ES2155854T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/26—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against hormones ; against hormone releasing or inhibiting factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Endocrinology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Cosmetics (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

LA PRESENTE INVENCION PROPORCIONA UN PROCESO PARA PRODUCIR PROTEINAS DE FUSION QUE COMPRENDEN FRAGMENTOS DE SCFV MEDIANTE UN MOLDE DE ASPERGILLUS TRANSFORMADO QUE CONTIENE UNA SECUENCIA DE ADN QUE CODIFICA EL FRAGMENTO SCFV BAJO EL CONTROL DE AL MENOS UNA EXPRESION Y/O UNA REGION DE REGULACION DE SECRECION DERIVADA DE UN MOLDE SELECCIONADO DEL GRUPO QUE CONSTA DE SECUENCIAS PROMOTORAS, SECUENCIAS TERMINADORAS Y SECUENCIAS DE ADN DE CODIFICACION DE SECUENCIA DE SEÑAL O DERIVADOS FUNCIONALES O ANALOGOS DE LAS MISMAS. TAL REGION DE REGULACION PUEDE DERIVARSE DEL GEN ENDOXILANASA II (GEN EXIA) DE ASPERGILLUS NIGER VAR. AWAMORI PRESENTE EN EL PLASMIDO PAW14B O PUEDE SER LA COMBINACION DE UN PROMOTOR Y UNA SECUENCIA DE ADN DE CODIFICACION DE SECUENCIA DE SEÑAL DERIVADA DE UN GEN DE GLUCOMILASA EX ASPERGILLUS MAS UNA SECUENCIA TERMINADORA DE UN GEN TRPC EX ASPERGILLUS. PREFERIBLEMENTE SE PRODUCE UNA PROTEINA DE FUSION QUE COMPRENDE "PROTEINA DE MOLDE SEGREGADA - (KEX2 -) SCFV". ADEMAS SE PROPORCIONAN NUEVOS PRODUCTOS QUE COMPRENDEN UN FRAGMENTO DE SCFV O PRODUCTO DE FUSION DEL MISMO, COMPOSICIONES, POR EJEMPLO, PRODUCTOS DE CONSUMIDOR, QUE CONTIENEN TANTO NUEVOS COMO ANTIGUOS PRODUCTOS ASI PRODUCIDOS. PREFERIBLEMENTE EL FRAGMENTO SCFV RECONOCE UN COMPUESTO PRESENTE EN EL ECOSISTEMA HUMANO, COMO MICROORGANISMOS O ENZIMAS. TALES COMPUESTOS PUEDEN ESTAR PRESENTES EN LA CAVIDAD ORAL, POR EJEMPLO, IMPLICADOS EN LA FORMACION DE PLACA, CARIES, GINGIVITIS, ENFERMEDADES PERIODONTALES, O MAL ALIENTO, O EN LA PIEL HUMANA, POR EJEMPLO, IMPLICADOS EN LA FORMACION DE MAL OLOR, INFLAMACION O PERDIDA DE PELO, O PUEDE SER UNA HORMONA, POR EJEMPLO, HCG.
ES94920443T 1993-06-09 1994-06-09 Procedimiento de produccion de proteinas de fusion que comprende fragmentos de scfv con la ayuda de un molde transformado. Expired - Lifetime ES2155854T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP93201660 1993-06-09
EP93201661 1993-06-09
EP93201706 1993-06-14

Publications (1)

Publication Number Publication Date
ES2155854T3 true ES2155854T3 (es) 2001-06-01

Family

ID=27235312

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94920443T Expired - Lifetime ES2155854T3 (es) 1993-06-09 1994-06-09 Procedimiento de produccion de proteinas de fusion que comprende fragmentos de scfv con la ayuda de un molde transformado.

Country Status (10)

Country Link
EP (1) EP0702721B1 (es)
JP (1) JPH08511160A (es)
AT (1) ATE198490T1 (es)
AU (1) AU7123494A (es)
CA (1) CA2162878A1 (es)
DE (1) DE69426527T2 (es)
DK (1) DK0702721T3 (es)
ES (1) ES2155854T3 (es)
FI (1) FI114870B (es)
WO (1) WO1994029457A2 (es)

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DK0826054T3 (da) * 1995-05-18 2004-04-19 Genencor Int Ekspression af glycosyltransferase i Aspergillus
AU8437398A (en) 1997-06-13 1998-12-30 Unilever Plc Bleaching enzymes
EP0954978B1 (en) 1998-03-12 2011-11-30 VHsquared Limited New products comprising inactivated yeasts or moulds provided with active antibodies
ATE535154T1 (de) 1998-03-12 2011-12-15 Vhsquared Ltd Produkten die inaktivierte hefen oder schimmel enthalten, die auf ihrer aussenoberfläche aktive antikörper haben
AU6153100A (en) 1999-07-27 2001-02-13 Davis, Paul James Bleaching detergent compositions
EP1368380B1 (en) 2000-12-19 2009-09-02 Unilever N.V. Stabilisation of heavy chain Camelid antibodies with potassium salts
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EP2557090A3 (en) 2006-12-19 2013-05-29 Ablynx N.V. Amino acid sequences directed against GPCRs and polypeptides comprising the same for the treatment of GPCR-related diseases and disorders
WO2008074840A2 (en) 2006-12-19 2008-06-26 Ablynx N.V. Amino acid sequences directed against a metalloproteinase from the adam family and polypeptides comprising the same for the treatment of adam-related diseases and disorders
WO2008081031A2 (en) * 2007-01-05 2008-07-10 Novozymes A/S Methods of increasing the production yield of a secreted antibody in a filamentous fungus by overexpression of the chaperone bip
EP2260058A2 (en) 2008-04-07 2010-12-15 Ablynx N.V. Single variable domains against the notch pathways
CN102388069B (zh) 2009-04-10 2016-01-20 埃博灵克斯股份有限公司 用于治疗il-6r相关疾病和病症的改进的针对il-6r的氨基酸序列和包含其的多肽
US9403898B2 (en) 2009-05-07 2016-08-02 Novozymes Biopharma Dk A/S Method for purifying albumin
RU2016103067A (ru) 2009-06-05 2018-11-20 Аблинкс Нв Улучшенные аминокислотные последовательности, направленные против респираторно-синцитиального вируса человека (hrsv), и полипептиды, включающие такие последовательности, для профилактики и/или лечения инфекций дыхательных путей
US9644022B2 (en) 2009-11-30 2017-05-09 Ablynx N.V. Amino acid sequences directed against human respiratory syncytial virus (HRSV) and polypeptides comprising the same for the prevention and/or treatment of respiratory tract infections
US8962807B2 (en) 2009-12-14 2015-02-24 Ablynx N.V. Single variable domain antibodies against OX40L, constructs and therapeutic use
WO2011083141A2 (en) 2010-01-08 2011-07-14 Ablynx Nv Method for generation of immunoglobulin sequences by using lipoprotein particles
US9120855B2 (en) 2010-02-10 2015-09-01 Novartis Ag Biologic compounds directed against death receptor 5
WO2011117423A1 (en) 2010-03-26 2011-09-29 Ablynx N.V. Immunoglobulin single variable domains directed against cxcr7
CN102906118B (zh) 2010-05-20 2017-07-28 埃博灵克斯股份有限公司 与her3相关的生物材料
EP2621953B1 (en) 2010-09-30 2017-04-05 Ablynx N.V. Biological materials related to c-met
WO2012062713A1 (en) 2010-11-08 2012-05-18 Novartis Ag Cxcr2 binding polypeptides
US20150158948A9 (en) 2011-03-28 2015-06-11 Francis Descamps Bispecific anti-cxcr7 immunoglobulin single variable domains
UA117218C2 (uk) 2011-05-05 2018-07-10 Мерк Патент Гмбх Поліпептид, спрямований проти il-17a, il-17f та/або il17-a/f
CN104271598A (zh) 2011-06-23 2015-01-07 埃博灵克斯股份有限公司 针对IgE的免疫球蛋白单可变结构域
CA2850261C (en) 2011-09-30 2021-04-20 Ablynx Nv C-met immunoglobulin single variable domains
US9328174B2 (en) 2012-05-09 2016-05-03 Novartis Ag Chemokine receptor binding polypeptides
WO2014087010A1 (en) 2012-12-07 2014-06-12 Ablynx N.V. IMPROVED POLYPEPTIDES DIRECTED AGAINST IgE
DK3071598T3 (da) 2013-05-29 2021-09-13 Vybion Inc Enkeltkædede intrabodies, der ændrer nedbrydning af huntingtin-mutant
NL2013661B1 (en) 2014-10-21 2016-10-05 Ablynx Nv KV1.3 Binding immunoglobulins.
WO2016097313A1 (en) 2014-12-19 2016-06-23 Ablynx N.V. Cysteine linked nanobody dimers
CN114920848A (zh) 2015-05-13 2022-08-19 埃博灵克斯股份有限公司 基于cd3反应性的t细胞募集多肽
HUE045437T2 (hu) 2015-05-13 2019-12-30 Ablynx Nv TCR-alfa-béta reaktivitásán alapulva T-sejtet toborzó polipeptidek
WO2017013270A1 (en) 2015-07-23 2017-01-26 Universite De Strasbourg Use of leptin signaling inhibitor for protecting kidneys from patients having ciliopathy
IL261188B (en) 2016-03-04 2022-08-01 Jn Biosciences Llc An anti-tigit antibody that binds to the tigit polypeptide on one or more amino acid residues
EP3526253A1 (en) 2016-10-14 2019-08-21 Institut Curie New anti-lsp1 antibody
WO2018091606A1 (en) 2016-11-16 2018-05-24 Ablynx Nv T cell recruiting polypeptides capable of binding cd123 and tcr alpha/beta
EP3624854A1 (en) 2017-05-16 2020-03-25 Université de Strasbourg Protein-drug conjugates and their use in the treatment of cancers
BR112019025097A2 (pt) 2017-06-02 2020-07-28 Merck Patent Gmbh imunoglobulinas de ligação a mmp13
KR20200013247A (ko) 2017-06-02 2020-02-06 아블린쓰 엔.브이. 아그레칸 결합 면역글로불린
JP7249961B2 (ja) 2017-06-02 2023-03-31 メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツング Ａｄａｍｔｓ５、ｍｍｐ１３およびアグリカンに結合するポリペプチド
EP3630847A1 (en) 2017-06-02 2020-04-08 Merck Patent GmbH Adamts binding immunoglobulins
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AR114269A1 (es) 2018-02-26 2020-08-12 Ablynx Nv Secuencias nucleotídicas mejoradas que codifican para péptidos de unión
KR101875564B1 (ko) * 2018-05-24 2018-07-09 국민대학교산학협력단 포도당 및 글리세롤을 이용한 유가식 배양을 통한 리시놀레산으로 오메가-하이드록시운덱-9-에논산의 생물전환공정
EP3636657A1 (en) 2018-10-08 2020-04-15 Ablynx N.V. Chromatography-free antibody purification method
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EP4263610A1 (en) 2020-12-18 2023-10-25 Ablynx N.V. T cell recruiting polypeptides based on tcr alpha/beta reactivity
EP4337691A1 (en) 2021-05-12 2024-03-20 Université de Strasbourg Single domain antibody specific for phosphorylated h2ax and its uses
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WO1993008300A1 (en) * 1991-10-18 1993-04-29 The University Of Calgary Expression-secretion vectors for the production of biologically active fv fragments
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Also Published As

Publication number Publication date
DE69426527D1 (de) 2001-02-08
AU7123494A (en) 1995-01-03
WO1994029457A2 (en) 1994-12-22
EP0702721A1 (en) 1996-03-27
DE69426527T2 (de) 2001-08-30
FI114870B (fi) 2005-01-14
JPH08511160A (ja) 1996-11-26
DK0702721T3 (da) 2001-05-21
CA2162878A1 (en) 1994-12-22
FI955446A0 (fi) 1995-11-13
FI955446A (fi) 1995-12-11
EP0702721B1 (en) 2001-01-03
ATE198490T1 (de) 2001-01-15
WO1994029457A3 (en) 1995-03-09

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