IT1270866B - Vettore ricombinante e suo impiego per la preparazione esocellulare di anticorpi in forma di singola molecola da bacillus subtilis - Google Patents
Vettore ricombinante e suo impiego per la preparazione esocellulare di anticorpi in forma di singola molecola da bacillus subtilisInfo
- Publication number
- IT1270866B IT1270866B ITMI930456A ITMI930456A IT1270866B IT 1270866 B IT1270866 B IT 1270866B IT MI930456 A ITMI930456 A IT MI930456A IT MI930456 A ITMI930456 A IT MI930456A IT 1270866 B IT1270866 B IT 1270866B
- Authority
- IT
- Italy
- Prior art keywords
- recombinant vector
- antibodies
- exocellular
- single molecule
- preparation
- Prior art date
Links
- 235000014469 Bacillus subtilis Nutrition 0.000 title abstract 4
- 244000063299 Bacillus subtilis Species 0.000 title 1
- 230000028327 secretion Effects 0.000 abstract 3
- 108090000145 Bacillolysin Proteins 0.000 abstract 1
- 102000035092 Neutral proteases Human genes 0.000 abstract 1
- 108091005507 Neutral proteases Proteins 0.000 abstract 1
- 108700026220 vif Genes Proteins 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/46—Hybrid immunoglobulins
- C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/832—Bacillus
- Y10S435/839—Bacillus subtilis
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/808—Materials and products related to genetic engineering or hybrid or fused cell technology, e.g. hybridoma, monoclonal products
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/867—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof involving immunoglobulin or antibody produced via recombinant dna technology
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
Si descrive un vettore ricombinante per l'espressione e secrezione in B.subtilis di anticorpi in forma di singola molecola (scFv), dove detto vettore comprende il promotore del gene della proteasi neutra, una nuova sequenza di secrezione (I) ed una sequenza di DNA che codifica per un anticorpo acFv di interesse, un ceppo di B.subtilis trasformato con detto vettore ricombinante ed un procedimento per la produzione esocellulare di anticorpi scFv mediante coltura di detto ceppo di B.subtilis.Il vettore ricombinante consente l'espressione di scFv in forma completamente solubile e la sua secrezione in rese elevate.
Priority Applications (11)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ITMI930456A IT1270866B (it) | 1993-03-10 | 1993-03-10 | Vettore ricombinante e suo impiego per la preparazione esocellulare di anticorpi in forma di singola molecola da bacillus subtilis |
DE69425843T DE69425843T2 (de) | 1993-03-10 | 1994-03-05 | Rekombinantes Vektor für die exozelluläre Verwendung von in Bacillus Subtilis exprimierten einkettigen Antikörpern |
AT94200564T ATE196317T1 (de) | 1993-03-10 | 1994-03-05 | Rekombinantes vektor für die exozelluläre verwendung von in bacillus subtilis exprimierten einkettigen antikörpern |
EP94200564A EP0614982B1 (en) | 1993-03-10 | 1994-03-05 | Recombinant vector for the exocellular preparation of single chain antibodies expressed in bacillus subtilis |
PT94200564T PT614982E (pt) | 1993-03-10 | 1994-03-05 | Vector recombinante e o seu uso para a preparacao exocelular de anticorpos sob a forma de molecula unica a partir de bacillus subtilis |
ES94200564T ES2149837T3 (es) | 1993-03-10 | 1994-03-05 | Vector recombinante para la preparacion exocelular de anticuerpos monocatenarios expresados en bacillus subtilis. |
DK94200564T DK0614982T3 (da) | 1993-03-10 | 1994-03-05 | Rekombinant vektor til exocellulær fremstilling af antistoffer i enkeltkædeform udtrykt i Bacillus subtilis |
US08/207,169 US5674712A (en) | 1993-03-10 | 1994-03-08 | Recombinant vector and use thereof for exocellular preparation of antibodies in single molecule form from Bacillus subtilis |
JP6066642A JPH0775581A (ja) | 1993-03-10 | 1994-03-10 | バチルス・ズブチリスの発現及び分泌組換えベクター |
US08/839,082 US20020016446A1 (en) | 1993-03-10 | 1997-04-23 | Recombinant vector and use thereof for exocellular preparation of single molecula antibodies from bacillus subtilis |
GR20000402393T GR3034704T3 (en) | 1993-03-10 | 2000-10-30 | Recombinant vector for the exocellular preparation of single chain antibodies expressed in bacillus subtilis. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ITMI930456A IT1270866B (it) | 1993-03-10 | 1993-03-10 | Vettore ricombinante e suo impiego per la preparazione esocellulare di anticorpi in forma di singola molecola da bacillus subtilis |
Publications (3)
Publication Number | Publication Date |
---|---|
ITMI930456A0 ITMI930456A0 (it) | 1993-03-10 |
ITMI930456A1 ITMI930456A1 (it) | 1994-09-10 |
IT1270866B true IT1270866B (it) | 1997-05-13 |
Family
ID=11365305
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ITMI930456A IT1270866B (it) | 1993-03-10 | 1993-03-10 | Vettore ricombinante e suo impiego per la preparazione esocellulare di anticorpi in forma di singola molecola da bacillus subtilis |
Country Status (10)
Country | Link |
---|---|
US (2) | US5674712A (it) |
EP (1) | EP0614982B1 (it) |
JP (1) | JPH0775581A (it) |
AT (1) | ATE196317T1 (it) |
DE (1) | DE69425843T2 (it) |
DK (1) | DK0614982T3 (it) |
ES (1) | ES2149837T3 (it) |
GR (1) | GR3034704T3 (it) |
IT (1) | IT1270866B (it) |
PT (1) | PT614982E (it) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1994029457A2 (en) * | 1993-06-09 | 1994-12-22 | Unilever N.V. | Process for producing fusion proteins comprising scfv fragments by a transformed mould |
US5665570A (en) * | 1994-02-10 | 1997-09-09 | Takeda Chemical Industries, Ltd. | Antibody-encoding recombinant DNA and its use |
US5891680A (en) * | 1995-02-08 | 1999-04-06 | Whitehead Institute For Biomedical Research | Bioactive fusion proteins comprising the p35 and p40 subunits of IL-12 |
US5801064A (en) * | 1995-12-04 | 1998-09-01 | Foresman; Mark D. | Assay methods and reagents for detecting autoantibodies |
CA2213512A1 (en) * | 1995-12-22 | 1997-07-03 | Toray Industries, Inc. | Method for producing bioactive fusion protein |
CA2257357C (en) | 1996-06-07 | 2010-04-13 | Neorx Corporation | Humanized antibodies with modified glycosylation |
ATE393912T1 (de) | 1998-02-04 | 2008-05-15 | Invitrogen Corp | Microarrays und ihre verwendungen |
US6576478B1 (en) * | 1998-07-14 | 2003-06-10 | Zyomyx, Inc. | Microdevices for high-throughput screening of biomolecules |
JP2004511753A (ja) | 2000-05-04 | 2004-04-15 | イエール ユニバーシティー | タンパク質活性のスクリーニング用タンパク質チップ |
EP1458411A4 (en) * | 2000-08-21 | 2004-09-22 | Smithkline Beecham Corp | ANTI-RANK LIGAND MONOCLONAL ANTIBODIES SUITABLE FOR THE TREATMENT OF RANK LIGAND RELATED DISORDERS |
AU2003210996A1 (en) * | 2002-02-11 | 2003-09-04 | Zymogenetics, Inc. | Materials and methods for preparing dimeric growth factors |
US20070148166A1 (en) * | 2002-05-13 | 2007-06-28 | Wu Madeline C S | Scfvs in photosynthetic microbes |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IT1206302B (it) * | 1987-06-26 | 1989-04-14 | Serono Cesare Ist Ricerca | Ormone follicolo-stimolante urinario |
IT1228925B (it) * | 1987-08-07 | 1991-07-10 | Eniricerche Spa | Procedimento per la preparazione dell'ormone della crescita umano |
IT1223577B (it) * | 1987-12-22 | 1990-09-19 | Eniricerche Spa | Procedimento migliorato per la preparazione dell'ormone della crescita umano naturale in forma pura |
IT1231156B (it) * | 1989-07-19 | 1991-11-19 | Eniricerche Spa | Espressione e secrezione di interleuchina 1 beta umana matura in bacillus subtilis e mezzi e metodi per il suo ottenimento. |
-
1993
- 1993-03-10 IT ITMI930456A patent/IT1270866B/it active IP Right Grant
-
1994
- 1994-03-05 AT AT94200564T patent/ATE196317T1/de not_active IP Right Cessation
- 1994-03-05 PT PT94200564T patent/PT614982E/pt unknown
- 1994-03-05 ES ES94200564T patent/ES2149837T3/es not_active Expired - Lifetime
- 1994-03-05 DK DK94200564T patent/DK0614982T3/da active
- 1994-03-05 EP EP94200564A patent/EP0614982B1/en not_active Expired - Lifetime
- 1994-03-05 DE DE69425843T patent/DE69425843T2/de not_active Expired - Fee Related
- 1994-03-08 US US08/207,169 patent/US5674712A/en not_active Expired - Fee Related
- 1994-03-10 JP JP6066642A patent/JPH0775581A/ja active Pending
-
1997
- 1997-04-23 US US08/839,082 patent/US20020016446A1/en not_active Abandoned
-
2000
- 2000-10-30 GR GR20000402393T patent/GR3034704T3/el not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
US20020016446A1 (en) | 2002-02-07 |
DE69425843D1 (de) | 2000-10-19 |
PT614982E (pt) | 2001-01-31 |
JPH0775581A (ja) | 1995-03-20 |
EP0614982B1 (en) | 2000-09-13 |
ITMI930456A0 (it) | 1993-03-10 |
EP0614982A1 (en) | 1994-09-14 |
DE69425843T2 (de) | 2001-03-29 |
GR3034704T3 (en) | 2001-01-31 |
DK0614982T3 (da) | 2000-12-18 |
ITMI930456A1 (it) | 1994-09-10 |
US5674712A (en) | 1997-10-07 |
ATE196317T1 (de) | 2000-09-15 |
ES2149837T3 (es) | 2000-11-16 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
0001 | Granted | ||
TA | Fee payment date (situation as of event date), data collected since 19931001 |
Effective date: 19970313 |