ATE196317T1 - Rekombinantes vektor für die exozelluläre verwendung von in bacillus subtilis exprimierten einkettigen antikörpern - Google Patents

Rekombinantes vektor für die exozelluläre verwendung von in bacillus subtilis exprimierten einkettigen antikörpern

Info

Publication number
ATE196317T1
ATE196317T1 AT94200564T AT94200564T ATE196317T1 AT E196317 T1 ATE196317 T1 AT E196317T1 AT 94200564 T AT94200564 T AT 94200564T AT 94200564 T AT94200564 T AT 94200564T AT E196317 T1 ATE196317 T1 AT E196317T1
Authority
AT
Austria
Prior art keywords
recombinant vector
exocellular
scfv
single chain
bacillus subtilis
Prior art date
Application number
AT94200564T
Other languages
English (en)
Inventor
Guido Grandi
Ferra Francesca De
Claudio Tosi
Ornella Tortora
Anna Cuzzoni
Original Assignee
Enitecnologie Spa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Enitecnologie Spa filed Critical Enitecnologie Spa
Application granted granted Critical
Publication of ATE196317T1 publication Critical patent/ATE196317T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/832—Bacillus
    • Y10S435/839—Bacillus subtilis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/808—Materials and products related to genetic engineering or hybrid or fused cell technology, e.g. hybridoma, monoclonal products
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/867—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof involving immunoglobulin or antibody produced via recombinant dna technology

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT94200564T 1993-03-10 1994-03-05 Rekombinantes vektor für die exozelluläre verwendung von in bacillus subtilis exprimierten einkettigen antikörpern ATE196317T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ITMI930456A IT1270866B (it) 1993-03-10 1993-03-10 Vettore ricombinante e suo impiego per la preparazione esocellulare di anticorpi in forma di singola molecola da bacillus subtilis

Publications (1)

Publication Number Publication Date
ATE196317T1 true ATE196317T1 (de) 2000-09-15

Family

ID=11365305

Family Applications (1)

Application Number Title Priority Date Filing Date
AT94200564T ATE196317T1 (de) 1993-03-10 1994-03-05 Rekombinantes vektor für die exozelluläre verwendung von in bacillus subtilis exprimierten einkettigen antikörpern

Country Status (10)

Country Link
US (2) US5674712A (de)
EP (1) EP0614982B1 (de)
JP (1) JPH0775581A (de)
AT (1) ATE196317T1 (de)
DE (1) DE69425843T2 (de)
DK (1) DK0614982T3 (de)
ES (1) ES2149837T3 (de)
GR (1) GR3034704T3 (de)
IT (1) IT1270866B (de)
PT (1) PT614982E (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0702721B1 (de) * 1993-06-09 2001-01-03 Unilever N.V. Verfahren zur herstellung von fusionsproteinen, die scfv fragmente enthalten, in transformierten schimmelpilzen
US5665570A (en) * 1994-02-10 1997-09-09 Takeda Chemical Industries, Ltd. Antibody-encoding recombinant DNA and its use
US5891680A (en) * 1995-02-08 1999-04-06 Whitehead Institute For Biomedical Research Bioactive fusion proteins comprising the p35 and p40 subunits of IL-12
US5801064A (en) * 1995-12-04 1998-09-01 Foresman; Mark D. Assay methods and reagents for detecting autoantibodies
WO1997023639A1 (en) * 1995-12-22 1997-07-03 Toray Industries, Inc. Process for producing biologically active fused proteins
WO1997046589A2 (en) 1996-06-07 1997-12-11 Neorx Corporation Humanized antibodies that bind to the same antigen as bound by antibody nr-lu-13, and their use in pretargeting methods
EP1965213A3 (de) * 1998-02-04 2009-07-15 Invitrogen Corporation Microarrays und ihre Verwendungen
US6576478B1 (en) * 1998-07-14 2003-06-10 Zyomyx, Inc. Microdevices for high-throughput screening of biomolecules
CA2408291C (en) 2000-05-04 2014-07-15 Yale University High density protein arrays for screening of protein activity
AU2001288342A1 (en) * 2000-08-21 2002-03-04 Smith Kline Beecham Corporation Anti-rank ligand monoclonal antibodies useful in treatment of rank ligand mediated disorders
AU2003210996A1 (en) * 2002-02-11 2003-09-04 Zymogenetics, Inc. Materials and methods for preparing dimeric growth factors
US20070148166A1 (en) * 2002-05-13 2007-06-28 Wu Madeline C S Scfvs in photosynthetic microbes

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1206302B (it) * 1987-06-26 1989-04-14 Serono Cesare Ist Ricerca Ormone follicolo-stimolante urinario
IT1228925B (it) * 1987-08-07 1991-07-10 Eniricerche Spa Procedimento per la preparazione dell'ormone della crescita umano
IT1223577B (it) * 1987-12-22 1990-09-19 Eniricerche Spa Procedimento migliorato per la preparazione dell'ormone della crescita umano naturale in forma pura
IT1231156B (it) * 1989-07-19 1991-11-19 Eniricerche Spa Espressione e secrezione di interleuchina 1 beta umana matura in bacillus subtilis e mezzi e metodi per il suo ottenimento.

Also Published As

Publication number Publication date
ES2149837T3 (es) 2000-11-16
DK0614982T3 (da) 2000-12-18
ITMI930456A0 (it) 1993-03-10
GR3034704T3 (en) 2001-01-31
DE69425843D1 (de) 2000-10-19
EP0614982A1 (de) 1994-09-14
PT614982E (pt) 2001-01-31
ITMI930456A1 (it) 1994-09-10
US5674712A (en) 1997-10-07
IT1270866B (it) 1997-05-13
JPH0775581A (ja) 1995-03-20
US20020016446A1 (en) 2002-02-07
DE69425843T2 (de) 2001-03-29
EP0614982B1 (de) 2000-09-13

Similar Documents

Publication Publication Date Title
EP0610046A3 (en) Expression vectors encoding bispecific fusion proteins and methods of producing biologically active bispesific fusion proteins in a mammalian cell.
PT1124941E (pt) Processo para recuperar polipeptidos heterologos a partir de celulas bacterianas
ATE196317T1 (de) Rekombinantes vektor für die exozelluläre verwendung von in bacillus subtilis exprimierten einkettigen antikörpern
EP1167537A4 (de) Verfahren zur Herstellung von monoklonalen Antikörpern
ATE506374T1 (de) Exprimierungskontrolle unter verwendung von variabelen intergenischen sequenzen
NZ334211A (en) Antibody fragment comprising a linear heavy chain associated with two light chain fragments
CA2203373A1 (en) Process for improved recombinant production of disulphide bond-containing polypeptides via a protein disulfide isomerase
EP1020528A3 (de) Verfahren zur Hestellung von ausgeschiedene Proteine
CY1110494T1 (el) Μεταστροφες μεταβολικου ρυθμου σε ζυμωσεις που εκφραζουν ανασυνδυασμενες πρωτεϊνες
DE3587524D1 (de) Herstellung von chimaeren antikoerpern.
DE3785186D1 (de) Bindungsmolekuele mit einzelpolypeptidkette.
EP0737747A3 (de) Cytoplasmatische Expression von Antikörpern, Antikörperfragmenten und Antikörperfragment-Fusionsmolekülen in E. coli
NO940736L (no) DNA sekvenser som koder for gelonin polypeptid
AU1515992A (en) Improvement of the yield when disulfide-bonded proteins are secreted
IL150676A0 (en) NOVEL STRa6 POLYPEPTIDES
PT742821E (pt) Processo de preparacao de uma biblioteca multicombinatoria de vectores de expressao de genes de anticorpos
AU7066187A (en) Enhanced protein production in bacteria by employing a novel ribosome binding site
GR3021040T3 (en) Process for production of stranger protein in streptomycetes
DE69534743D1 (de) FÜR DAS IgG-Fc-BINDENDE PROTEIN KODIERENDES GEN
DK203588A (da) Fremgangsmaade til fremstilling af proteiner
WO1994011507A3 (en) Production of monoclonal recombinant antibodies without the use of hybridomas by in vitro spleen fragment culture combined with isothermal self-sustained sequence replication of rna
WO1997039130A3 (en) Method for expressing and secreting keratinase
JPS6434290A (en) Hybrid promoter

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee