WO1997039130A3 - Method for expressing and secreting keratinase - Google Patents

Method for expressing and secreting keratinase Download PDF

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Publication number
WO1997039130A3
WO1997039130A3 PCT/US1997/006477 US9706477W WO9739130A3 WO 1997039130 A3 WO1997039130 A3 WO 1997039130A3 US 9706477 W US9706477 W US 9706477W WO 9739130 A3 WO9739130 A3 WO 9739130A3
Authority
WO
WIPO (PCT)
Prior art keywords
keratinase
host cell
secreting
expressing
keratinase enzyme
Prior art date
Application number
PCT/US1997/006477
Other languages
French (fr)
Other versions
WO1997039130A2 (en
Inventor
Jason C H Shih
Xiang Lin
Sui-Lam Wong
Original Assignee
Univ North Carolina
Univ Technologies Int
Jason C H Shih
Xiang Lin
Sui-Lam Wong
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ North Carolina, Univ Technologies Int, Jason C H Shih, Xiang Lin, Sui-Lam Wong filed Critical Univ North Carolina
Priority to AU31144/97A priority Critical patent/AU3114497A/en
Publication of WO1997039130A2 publication Critical patent/WO1997039130A2/en
Publication of WO1997039130A3 publication Critical patent/WO1997039130A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention provides a Bacillus subtilis host cell capable of expressing and secreting keratinase. The host cell contains a recombinant DNA molecule comprising vector DNA and DNA encoding Bacillus licheniformis PWD-1 keratinase enzyme operatively associated therewith. The present invention also provides a method for producing keratinase enzyme. The method includes the steps of (a) culturing a Bacillus subtilis host cell containing a recombinant DNA molecule comprising vector DNA and DNA encoding Bacillus licheniformis PWD-1 keratinase enzyme operatively associated therewith, and (b) collecting keratinase enzyme from the cell culture.
PCT/US1997/006477 1996-04-18 1997-04-16 Method for expressing and secreting keratinase WO1997039130A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU31144/97A AU3114497A (en) 1996-04-18 1997-04-16 Method for expressing and secreting keratinase

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US63444096A 1996-04-18 1996-04-18
US08/634,440 1996-04-18

Publications (2)

Publication Number Publication Date
WO1997039130A2 WO1997039130A2 (en) 1997-10-23
WO1997039130A3 true WO1997039130A3 (en) 1998-02-12

Family

ID=24543801

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1997/006477 WO1997039130A2 (en) 1996-04-18 1997-04-16 Method for expressing and secreting keratinase

Country Status (3)

Country Link
AU (1) AU3114497A (en)
WO (1) WO1997039130A2 (en)
ZA (1) ZA973310B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2444215T3 (en) 2002-07-29 2014-02-24 Zymtech Production As Method for the production of peptides and amino acids from material of animal origin comprising proteins
WO2004034776A2 (en) 2002-08-09 2004-04-29 North Carolina State University Methods and compositions for improving growth of meat-type poultry
EP1546384A4 (en) * 2002-09-13 2006-03-29 Univ North Carolina State Construction of bacillus licheniformis t1 strain and fermentation production of crude enzyme extract therefrom

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0310137A2 (en) * 1987-10-02 1989-04-05 Zymogenetics, Inc. BAR1 secretion signal
WO1989009278A1 (en) * 1988-03-31 1989-10-05 North Carolina State University Method of degrading keratinaceous material and bacteria useful therefor
WO1995033056A1 (en) * 1994-05-27 1995-12-07 North Carolina State University Dna encoding the bacillus licheniformis pwd-1 keratinase

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0310137A2 (en) * 1987-10-02 1989-04-05 Zymogenetics, Inc. BAR1 secretion signal
WO1989009278A1 (en) * 1988-03-31 1989-10-05 North Carolina State University Method of degrading keratinaceous material and bacteria useful therefor
WO1995033056A1 (en) * 1994-05-27 1995-12-07 North Carolina State University Dna encoding the bacillus licheniformis pwd-1 keratinase

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
LIN X ET AL: "Gene sequence and expression of keratinase in the feather-degrading Bacillus licheniformis PWD -1.", SIXTEENTH ANNUAL MEETING OF THE SOUTHERN POULTRY SCIENCE SOCIETY, ATLANTA, GEORGIA, USA, JANUARY 18-20, 1995. POULTRY SCIENCE 74 (SUPPL. 1). 1995. 201. ISSN: 0032-5791, XP002042753 *
PEI-ZHI WANG ET AL.: "Overlapping promoters transcribed by Bacillus subtilis sigma55 and sigma37 RNA polymerase holoenzymes during growth and stationary phases", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 259, no. 13, 10 July 1984 (1984-07-10), MD US, pages 8619 - 8625, XP002042749 *
XIANG LIN ET AL.: "Nucleotide sequence and expression of kerA, the gene encoding a keratinolytic protease of Bacillus licheniformis PWD-1", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 61, no. 4, April 1995 (1995-04-01), pages 1469 - 1474, XP002042752 *

Also Published As

Publication number Publication date
AU3114497A (en) 1997-11-07
ZA973310B (en) 1998-07-30
WO1997039130A2 (en) 1997-10-23

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