ES2140372T3 - Metodo y equipo para detectar un acido nucleico diana usando sondas de captura inmovilizadas sobre un soporte de poliestireno por una proteina intermedia. - Google Patents
Metodo y equipo para detectar un acido nucleico diana usando sondas de captura inmovilizadas sobre un soporte de poliestireno por una proteina intermedia.Info
- Publication number
- ES2140372T3 ES2140372T3 ES90118620T ES90118620T ES2140372T3 ES 2140372 T3 ES2140372 T3 ES 2140372T3 ES 90118620 T ES90118620 T ES 90118620T ES 90118620 T ES90118620 T ES 90118620T ES 2140372 T3 ES2140372 T3 ES 2140372T3
- Authority
- ES
- Spain
- Prior art keywords
- daily
- detect
- nucleic acid
- equipment
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6832—Enhancement of hybridisation reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Dental Preparations (AREA)
- Transplanting Machines (AREA)
Abstract
SE DESCRIBE UN FORMATO PARA CAPTAR POR HIBRIDACION, UN DNA AMPLIFICADO PCR SOBRE UN SOLIDO SOPORTE. TRAS MARBETEAR EL DNA AMPLIFICADO CON BIOTIN, ESTE ES CAPTURADO POR LA HIBRIDACION PAR-BASE DE UNA SONDA ESPECIFICA DE UN OLIGONUCLEOTICO QUE SE UNE A UN SOPORTE SOLIDO, Y DE ESTA FORMA, SE DETECTA EL DNA CON UN ENSAYO DE DETECCION CROMOGENICA DEPENDIENTE DE BIOTIN. EN UN FORMATO ESPECIFICO ELEGIDO, SE DESCRIBEN LAS SONDAS QUE HACEN POSIBLE LA DETECCION DE CLAMIDIA TRACOMATIS.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US07/414,542 US5232829A (en) | 1989-09-29 | 1989-09-29 | Detection of chlamydia trachomatis by polymerase chain reaction using biotin labelled lina primers and capture probes |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2140372T3 true ES2140372T3 (es) | 2000-03-01 |
Family
ID=23641905
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES90118620T Expired - Lifetime ES2140372T3 (es) | 1989-09-29 | 1990-09-27 | Metodo y equipo para detectar un acido nucleico diana usando sondas de captura inmovilizadas sobre un soporte de poliestireno por una proteina intermedia. |
ES98111076T Expired - Lifetime ES2275292T3 (es) | 1989-09-29 | 1990-09-27 | Sondas y equipos para la deteccion de chlamidia trachomatis. |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES98111076T Expired - Lifetime ES2275292T3 (es) | 1989-09-29 | 1990-09-27 | Sondas y equipos para la deteccion de chlamidia trachomatis. |
Country Status (14)
Country | Link |
---|---|
US (1) | US5232829A (es) |
EP (2) | EP0875583B1 (es) |
JP (1) | JP2719225B2 (es) |
AT (2) | ATE345398T1 (es) |
AU (2) | AU6329090A (es) |
BR (1) | BR9004881A (es) |
CA (1) | CA2026280C (es) |
DE (2) | DE69033387T2 (es) |
DK (2) | DK0420260T3 (es) |
ES (2) | ES2140372T3 (es) |
IL (1) | IL95800A (es) |
NO (1) | NO302204B1 (es) |
NZ (2) | NZ235463A (es) |
ZA (1) | ZA907706B (es) |
Families Citing this family (101)
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US5753433A (en) * | 1909-12-05 | 1998-05-19 | Boehringer Mannheim Gmbh | Method for the sensitive detection of nucleic acids |
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US5800992A (en) | 1989-06-07 | 1998-09-01 | Fodor; Stephen P.A. | Method of detecting nucleic acids |
US6309822B1 (en) | 1989-06-07 | 2001-10-30 | Affymetrix, Inc. | Method for comparing copy number of nucleic acid sequences |
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US6551784B2 (en) | 1989-06-07 | 2003-04-22 | Affymetrix Inc | Method of comparing nucleic acid sequences |
US6040138A (en) * | 1995-09-15 | 2000-03-21 | Affymetrix, Inc. | Expression monitoring by hybridization to high density oligonucleotide arrays |
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US5695926A (en) * | 1990-06-11 | 1997-12-09 | Bio Merieux | Sandwich hybridization assays using very short capture probes noncovalently bound to a hydrophobic support |
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JPH07107537B2 (ja) * | 1987-08-03 | 1995-11-15 | イーストマン コダック カンパニー | アビジン−及びビオチン−固定試薬,分析要素並びにその使用法 |
CA1339351C (en) * | 1987-10-15 | 1997-08-26 | Michael S. Urdea | Nucleic acid multimers and amplified nucleic acid hybridization assays using same |
IT1224253B (it) * | 1988-04-08 | 1990-09-26 | Sclavo Spa | Oligonucleotidi sintetici utili per la determinazione di chlamydia trachomatis in un campione biologico |
ATE173508T1 (de) * | 1988-05-20 | 1998-12-15 | Hoffmann La Roche | Befestigung von sequenzspezifischen proben |
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CA2031659A1 (en) * | 1990-01-26 | 1991-07-27 | John B. Findlay | Water-insoluble reagent, nucleic acid probe, test kit and diagnostic and purification methods |
-
1989
- 1989-09-29 US US07/414,542 patent/US5232829A/en not_active Expired - Lifetime
-
1990
- 1990-09-26 NZ NZ235463A patent/NZ235463A/en unknown
- 1990-09-26 IL IL9580090A patent/IL95800A/en not_active IP Right Cessation
- 1990-09-26 CA CA002026280A patent/CA2026280C/en not_active Expired - Lifetime
- 1990-09-26 ZA ZA907706A patent/ZA907706B/xx unknown
- 1990-09-26 NZ NZ247522A patent/NZ247522A/en unknown
- 1990-09-27 AU AU63290/90A patent/AU6329090A/en not_active Abandoned
- 1990-09-27 DK DK90118620T patent/DK0420260T3/da active
- 1990-09-27 AT AT98111076T patent/ATE345398T1/de not_active IP Right Cessation
- 1990-09-27 DE DE69033387T patent/DE69033387T2/de not_active Expired - Lifetime
- 1990-09-27 EP EP98111076A patent/EP0875583B1/en not_active Expired - Lifetime
- 1990-09-27 ES ES90118620T patent/ES2140372T3/es not_active Expired - Lifetime
- 1990-09-27 AT AT90118620T patent/ATE187499T1/de not_active IP Right Cessation
- 1990-09-27 DE DE69034232T patent/DE69034232T2/de not_active Expired - Lifetime
- 1990-09-27 DK DK98111076T patent/DK0875583T3/da active
- 1990-09-27 ES ES98111076T patent/ES2275292T3/es not_active Expired - Lifetime
- 1990-09-27 EP EP90118620A patent/EP0420260B1/en not_active Expired - Lifetime
- 1990-09-28 NO NO904240A patent/NO302204B1/no not_active IP Right Cessation
- 1990-09-28 BR BR909004881A patent/BR9004881A/pt not_active Application Discontinuation
- 1990-09-29 JP JP2262934A patent/JP2719225B2/ja not_active Expired - Lifetime
-
1994
- 1994-09-02 AU AU72804/94A patent/AU685144B2/en not_active Expired
Also Published As
Publication number | Publication date |
---|---|
EP0875583A2 (en) | 1998-11-04 |
DE69033387T2 (de) | 2000-03-30 |
IL95800A (en) | 1994-10-07 |
ES2275292T3 (es) | 2007-06-01 |
DK0420260T3 (da) | 2000-03-27 |
JP2719225B2 (ja) | 1998-02-25 |
ATE345398T1 (de) | 2006-12-15 |
CA2026280C (en) | 2003-04-15 |
DE69034232D1 (de) | 2006-12-28 |
AU7280494A (en) | 1994-11-24 |
ATE187499T1 (de) | 1999-12-15 |
BR9004881A (pt) | 1991-09-10 |
DE69033387D1 (de) | 2000-01-13 |
US5232829A (en) | 1993-08-03 |
EP0420260A2 (en) | 1991-04-03 |
EP0420260B1 (en) | 1999-12-08 |
EP0875583B1 (en) | 2006-11-15 |
NZ235463A (en) | 1994-03-25 |
CA2026280A1 (en) | 1991-03-30 |
AU6329090A (en) | 1991-04-11 |
EP0420260A3 (en) | 1991-09-18 |
AU685144B2 (en) | 1998-01-15 |
DK0875583T3 (da) | 2007-02-26 |
IL95800A0 (en) | 1991-06-30 |
EP0875583A3 (en) | 1999-02-17 |
NZ247522A (en) | 1994-03-25 |
NO904240L (no) | 1991-04-02 |
NO302204B1 (no) | 1998-02-02 |
DE69034232T2 (de) | 2007-03-01 |
JPH03206898A (ja) | 1991-09-10 |
ZA907706B (en) | 1991-06-26 |
NO904240D0 (no) | 1990-09-28 |
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