WO2001053817A3 - Methods of and apparatus for separating and detecting nucleic acid - Google Patents

Methods of and apparatus for separating and detecting nucleic acid Download PDF

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Publication number
WO2001053817A3
WO2001053817A3 PCT/US2001/001963 US0101963W WO0153817A3 WO 2001053817 A3 WO2001053817 A3 WO 2001053817A3 US 0101963 W US0101963 W US 0101963W WO 0153817 A3 WO0153817 A3 WO 0153817A3
Authority
WO
WIPO (PCT)
Prior art keywords
gel
analyte
separating
methods
detection
Prior art date
Application number
PCT/US2001/001963
Other languages
French (fr)
Other versions
WO2001053817A9 (en
WO2001053817A2 (en
Inventor
Chris L Jones
Ezra S Abrams
Stephen G Kieffer-Higgins
Tianhong Zhang
Graham P Eacock
Michael J Webb
Christopher Mcdowell
Original Assignee
Mosaic Technologies
Chris L Jones
Ezra S Abrams
Kieffer Higgins Stephen G
Tianhong Zhang
Graham P Eacock
Michael J Webb
Christopher Mcdowell
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mosaic Technologies, Chris L Jones, Ezra S Abrams, Kieffer Higgins Stephen G, Tianhong Zhang, Graham P Eacock, Michael J Webb, Christopher Mcdowell filed Critical Mosaic Technologies
Priority to AU31028/01A priority Critical patent/AU3102801A/en
Priority to CA002397883A priority patent/CA2397883A1/en
Priority to JP2001554055A priority patent/JP2003520961A/en
Priority to EP01903178A priority patent/EP1255983A2/en
Publication of WO2001053817A2 publication Critical patent/WO2001053817A2/en
Publication of WO2001053817A3 publication Critical patent/WO2001053817A3/en
Publication of WO2001053817A9 publication Critical patent/WO2001053817A9/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A thin gel for separating and detecting a target molecule, (e.g. an analyte) and methods of use thereof is described. The gel can be optionally reinforced to facilitate removal from an electrophoretic apparatus, wherein the gel matrix comprises immobilized (e.g., covalently bound) capture probes capable of specifically hybridizing with, or binding to, the analyte to be detected. The gel is thin and very short assay times can be achieved facilitating rapid detection of analytes. An assay apparatus is suitable for use with an electrophoretic assay system for the detection of, or separation and detection of an analyte in a biological sample is also described.
PCT/US2001/001963 2000-01-19 2001-01-18 Methods of and apparatus for separating and detecting nucleic acid WO2001053817A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU31028/01A AU3102801A (en) 2000-01-19 2001-01-18 Methods of and apparatus for separating and detecting nucleic acid
CA002397883A CA2397883A1 (en) 2000-01-19 2001-01-18 Methods of and apparatus for separating and detecting nucleic acid
JP2001554055A JP2003520961A (en) 2000-01-19 2001-01-18 Methods and devices for separating and detecting nucleic acids
EP01903178A EP1255983A2 (en) 2000-01-19 2001-01-18 Methods of and apparatus for separating and detecting nucleic acid

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US17683900P 2000-01-19 2000-01-19
US60/176,839 2000-01-19

Publications (3)

Publication Number Publication Date
WO2001053817A2 WO2001053817A2 (en) 2001-07-26
WO2001053817A3 true WO2001053817A3 (en) 2002-02-07
WO2001053817A9 WO2001053817A9 (en) 2003-01-09

Family

ID=22646067

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/001963 WO2001053817A2 (en) 2000-01-19 2001-01-18 Methods of and apparatus for separating and detecting nucleic acid

Country Status (5)

Country Link
EP (1) EP1255983A2 (en)
JP (1) JP2003520961A (en)
AU (1) AU3102801A (en)
CA (1) CA2397883A1 (en)
WO (1) WO2001053817A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE335845T1 (en) 1997-05-16 2006-09-15 Exact Sciences Corp ELECTROPHORETIC ANALYSIS OF MOLECULES USING IMMOBILIZED PROBE
US6911308B2 (en) 2001-01-05 2005-06-28 Exact Sciences Corporation Methods for detecting, grading or monitoring an H. pylori infection
GB0212853D0 (en) * 2002-06-01 2002-07-17 Novartis Pharma Ag Separation of molecules
WO2005031335A1 (en) * 2003-09-26 2005-04-07 Mitsubishi Rayon Co., Ltd. Cataphoresis apparatus, cataphoresis method, and detection method for organism-related material using the apparatus and the method
JP5544759B2 (en) * 2009-05-21 2014-07-09 株式会社島津製作所 Sample analysis method using mass spectrometry
KR20150022785A (en) * 2012-05-31 2015-03-04 지이 헬스케어 바이오-사이언시스 에이비 An electrophoresis system and a separation and identification method
EP3978914A1 (en) * 2020-09-30 2022-04-06 iCare Diagnostics International Co. Ltd. Electrode, ph measuring device, and electrophoresis detection system

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4164464A (en) * 1977-03-25 1979-08-14 Instrumentation Specialties Company Sample concentrator
WO1993013412A1 (en) * 1991-12-31 1993-07-08 E.I. Du Pont De Nemours And Company Apparatus for direct blotting and automated electrophoresis, transfer and detection and processes utilizing the apparatus thereof
WO1994009185A1 (en) * 1992-10-14 1994-04-28 Labintelligence, Inc. Electrophoretic quantitation of specific binding complexes
US5348633A (en) * 1993-01-22 1994-09-20 Northeastern University Method for quantitating trace amounts of an analyte in a sample by affinity capillary electrophoresis
US5538614A (en) * 1994-08-17 1996-07-23 Han; Dawn D. Macromolecule recovery cassette

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4164464A (en) * 1977-03-25 1979-08-14 Instrumentation Specialties Company Sample concentrator
US4164464B1 (en) * 1977-03-25 1992-05-12 Instrumentation Specialties Co
WO1993013412A1 (en) * 1991-12-31 1993-07-08 E.I. Du Pont De Nemours And Company Apparatus for direct blotting and automated electrophoresis, transfer and detection and processes utilizing the apparatus thereof
WO1994009185A1 (en) * 1992-10-14 1994-04-28 Labintelligence, Inc. Electrophoretic quantitation of specific binding complexes
US5348633A (en) * 1993-01-22 1994-09-20 Northeastern University Method for quantitating trace amounts of an analyte in a sample by affinity capillary electrophoresis
US5538614A (en) * 1994-08-17 1996-07-23 Han; Dawn D. Macromolecule recovery cassette

Also Published As

Publication number Publication date
CA2397883A1 (en) 2001-07-26
WO2001053817A9 (en) 2003-01-09
AU3102801A (en) 2001-07-31
JP2003520961A (en) 2003-07-08
EP1255983A2 (en) 2002-11-13
WO2001053817A2 (en) 2001-07-26

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