WO2003066896A3 - Method for the detection of mutations - Google Patents

Method for the detection of mutations Download PDF

Info

Publication number
WO2003066896A3
WO2003066896A3 PCT/EP2003/001258 EP0301258W WO03066896A3 WO 2003066896 A3 WO2003066896 A3 WO 2003066896A3 EP 0301258 W EP0301258 W EP 0301258W WO 03066896 A3 WO03066896 A3 WO 03066896A3
Authority
WO
WIPO (PCT)
Prior art keywords
oligos
linkage
complex
mutations
analysis
Prior art date
Application number
PCT/EP2003/001258
Other languages
German (de)
French (fr)
Other versions
WO2003066896A2 (en
Inventor
Christian Albrecht
Filipp Oesterhelt
Boris Steipe
Hermann Gaub
Hauke Clausen-Schaumann
Michael Cieplik
Original Assignee
Nanotype Gmbh
Christian Albrecht
Filipp Oesterhelt
Boris Steipe
Hermann Gaub
Hauke Clausen-Schaumann
Michael Cieplik
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE2002105419 external-priority patent/DE10205419A1/en
Priority claimed from DE2002107918 external-priority patent/DE10207918A1/en
Application filed by Nanotype Gmbh, Christian Albrecht, Filipp Oesterhelt, Boris Steipe, Hermann Gaub, Hauke Clausen-Schaumann, Michael Cieplik filed Critical Nanotype Gmbh
Priority to EP03737328A priority Critical patent/EP1565569A2/en
Priority to AU2003244501A priority patent/AU2003244501A1/en
Publication of WO2003066896A2 publication Critical patent/WO2003066896A2/en
Publication of WO2003066896A3 publication Critical patent/WO2003066896A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method for the detection of mutations in nucleic acids is disclosed, comprising a) a number of nucleic acid strands for analysis are brought into contact with at least two types of oligonucleotides, receptor oligos and probe oligos, under conditions which bring about hybridisation of the oligos with the DNA for analysis to give a sample complex and a reference complex in such a manner that a double-sided linkage of sample complex and reference complex is produced which is or may be immobilised, b) the linkage is then optionally immobilised, c) a tensile force is applied to the linkage until one of the complexes separates and d) whichever of the complexes has separated is determined.
PCT/EP2003/001258 2002-02-09 2003-02-07 Method for the detection of mutations WO2003066896A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP03737328A EP1565569A2 (en) 2002-02-09 2003-02-07 Method for the detection of mutations
AU2003244501A AU2003244501A1 (en) 2002-02-09 2003-02-07 Method for the detection of mutations

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE10205419.3 2002-02-09
DE2002105419 DE10205419A1 (en) 2002-02-09 2002-02-09 Method for detecting nucleic acid mutations, useful e.g. for diagnosis and HLA typing, based on the force required to separate matched and mismatched hybrids
DE10207918.8 2002-02-23
DE2002107918 DE10207918A1 (en) 2002-02-23 2002-02-23 Method for detecting nucleic acid mutations, useful e.g. for diagnosis and HLA typing, based on the force required to separate matched and mismatched hybrids

Publications (2)

Publication Number Publication Date
WO2003066896A2 WO2003066896A2 (en) 2003-08-14
WO2003066896A3 true WO2003066896A3 (en) 2004-03-25

Family

ID=27735663

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/001258 WO2003066896A2 (en) 2002-02-09 2003-02-07 Method for the detection of mutations

Country Status (3)

Country Link
EP (1) EP1565569A2 (en)
AU (1) AU2003244501A1 (en)
WO (1) WO2003066896A2 (en)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10126798A1 (en) 2001-06-01 2002-12-19 Nanotype Gmbh Highly parallel determination of analytes, useful e.g. for diagnostic detection of proteins, by immobilization then contacting with surface carrying specific probes
KR100580644B1 (en) * 2004-02-16 2006-05-16 삼성전자주식회사 A method for noncovalently immobilizing a biomolecule on a solid substrate and a microarray produced by the same
EP2390350A1 (en) 2010-05-27 2011-11-30 Centre National de la Recherche Scientifique (CNRS) Method of DNA sequencing by polymerisation
EP2390351A1 (en) 2010-05-27 2011-11-30 Centre National de la Recherche Scientifique (CNRS) Method of DNA sequencing by hybridisation
FR2993282B1 (en) 2012-07-13 2017-11-10 Expanscience Lab METHOD FOR IDENTIFYING MOLECULAR MARKERS OF CHILD'S SKIN
FR3001889B1 (en) 2013-02-11 2021-02-12 Expanscience Lab USE OF A COMPOSITION CONTAINING AVOCADO PERSEOSE IN THE PROTECTION OF EPIDERMAL STEM CELLS.
FR3011008B1 (en) 2013-09-24 2017-12-29 Expanscience Lab METHODS OF EVALUATING DELETER EFFECTS OF UV ON CHILD'S SKIN
EP2886663A1 (en) 2013-12-19 2015-06-24 Centre National de la Recherche Scientifique (CNRS) Nanopore sequencing using replicative polymerases and helicases
FR3016373B1 (en) 2014-01-10 2018-01-19 Laboratoires Expanscience MAMMELON SKIN MODEL RECONSTITUTES
FR3019186B1 (en) 2014-03-31 2019-06-07 Laboratoires Expanscience METHODS OF EVALUATING THE DELETE EFFECTS OF URINE ON CHILDREN'S SKIN
FR3045669B1 (en) 2015-12-16 2019-04-05 Laboratoires Expanscience METHODS OF EVALUATING THE EFFECTS OF DEHYDRATION ON CHILDREN'S SKIN
FR3053053B1 (en) 2016-06-23 2018-07-13 Laboratoires Expanscience MODELS OF ATOPIC JUVENILE DERMATITIS
FR3059550B1 (en) 2016-12-01 2020-01-03 Universite De Rouen Normandie TREATMENT OF DISORDERS CAUSED BY FETAL ALCOHOLIZATION (TCAF)
FR3068045B1 (en) 2017-06-22 2021-06-04 Expanscience Lab RECONSTITUTED SENSITIVE SKIN MODELS
FR3081707A1 (en) 2018-05-30 2019-12-06 Universite De Rouen Normandie TREATMENT OF NEUROLOGICAL DISORDERS CAUSED BY FETAL ALCOHOLIZATION
CA3106418A1 (en) 2018-07-20 2020-01-23 Pierre Fabre Medicament Receptor for vista
FR3091351B1 (en) 2018-12-27 2021-05-21 Univ Rouen Centre Hospitalier FABRY'S DISEASE BIOMARKER
FR3098405B1 (en) 2019-07-12 2021-06-25 Expanscience Lab Composition comprising passionflower seed polyphenols, avocado peptides and witch hazel extract and use to treat and / or prevent stretch marks
EP3922641A1 (en) 2020-06-09 2021-12-15 Institut Gustave-Roussy Methods for detecting and treating covid patients requiring intensive care
FR3133198A1 (en) 2022-03-04 2023-09-08 Pierre Fabre Dermo-Cosmetique RECONSTITUTED SKIN MODEL

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6342353B1 (en) * 1997-02-21 2002-01-29 Centre National De La Recherche Scientifique (Cnrs) Method for characterizing nucleic acid duplex
WO2002014862A2 (en) * 2000-08-11 2002-02-21 Nanotype Gmbh Method and device for characterising and/or for detecting a bonding complex

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6342353B1 (en) * 1997-02-21 2002-01-29 Centre National De La Recherche Scientifique (Cnrs) Method for characterizing nucleic acid duplex
WO2002014862A2 (en) * 2000-08-11 2002-02-21 Nanotype Gmbh Method and device for characterising and/or for detecting a bonding complex

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ALBRECHT CHRISTIAN ET AL: "DNA: a programmable force sensor.", SCIENCE. UNITED STATES 18 JUL 2003, vol. 301, no. 5631, 18 July 2003 (2003-07-18), pages 367 - 370, XP002256755, ISSN: 1095-9203 *
NOY A ET AL: "STRETCHING AND BREAKING DUPLEX DNA BY CHEMICAL FORCE MICROSCOPY", CHEMISTRY AND BIOLOGY, CURRENT BIOLOGY, LONDON, GB, vol. 4, no. 7, 1 July 1997 (1997-07-01), pages 519 - 527, XP002071033, ISSN: 1074-5521 *
STRUNZ TORSTEN ET AL: "Dynamic force spectroscopy of single DNA molecules", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 96, no. 20, 28 September 1999 (1999-09-28), Sept. 28, 1999, pages 11277 - 11282, XP002256754, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
AU2003244501A1 (en) 2003-09-02
EP1565569A2 (en) 2005-08-24
WO2003066896A2 (en) 2003-08-14

Similar Documents

Publication Publication Date Title
WO2003066896A3 (en) Method for the detection of mutations
WO2000077260A8 (en) Genomic profiling: a rapid method for testing a complex biological sample for the presence of many types of organisms
D’Agata et al. Ultrasensitive detection of non-amplified genomic DNA by nanoparticle-enhanced surface plasmon resonance imaging
EP0360940A3 (en) Polynucleotide determination with selectable cleavage sites
WO2006007567A3 (en) Methods and compositions to detect nucleic acids in a biological sample
WO2004046687A3 (en) Methods of biosensing using fluorescent polymers and quencher-tether-ligand bioconjugates
AU6704596A (en) Method for detecting nucleic acids using nucleotide probes permitting both specific capture and detection
WO2000049182A3 (en) Analytical methods and materials for nucleic acid detection
WO2002055985A3 (en) Methods and reagents for identifying rare fetal cells in the material circulation
WO2006077102A3 (en) Method for the detection of hpv and probes, primers and kits
WO2001007640A3 (en) Method for detecting single nucleotide polymorphisms
EP1448581A4 (en) Methods for detection and quantification of analytes in complex mixtures
WO2006076047A3 (en) Electrocatalytic nucleic acid hybridization detection
CA2172248A1 (en) Self-sustained sequence replication electrochemiluminescent nucleic acid assay
WO2001016378A3 (en) Chromosome-wide analysis of protein-dna interactions
WO2006078660A3 (en) Nucleic acid enzyme ligation sensor
WO2004111271A3 (en) Means and method for the detection of target nucleotide sequences using ligation assays with improved oligonucleotide probe pairs
WO2006088910A3 (en) Sequence-specific detection of nucleotide sequences
WO2005001113A3 (en) Methods for detecting nucleic acid variations
WO2003033735A3 (en) Test strip assay system and method for the detection of specific nucleic acid sequences
WO2007056332A3 (en) Molecular diagnosis of autoimmune diseases
JP2006129866A5 (en)
WO2000068421A3 (en) Method for detecting microorganisms in a sample
WO2002070736A3 (en) Method for the detection of nucleic acid molecules
WO2001029264A3 (en) Nucleic acid primers and probes for detecting tumor cells

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SC SD SE SG SK SL TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWE Wipo information: entry into national phase

Ref document number: 2003737328

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2003737328

Country of ref document: EP

NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP

WWW Wipo information: withdrawn in national office

Ref document number: 2003737328

Country of ref document: EP