ES2119222T3 - Aislamiento de polipeptidos en fases acuosas multiples. - Google Patents

Aislamiento de polipeptidos en fases acuosas multiples.

Info

Publication number
ES2119222T3
ES2119222T3 ES94925830T ES94925830T ES2119222T3 ES 2119222 T3 ES2119222 T3 ES 2119222T3 ES 94925830 T ES94925830 T ES 94925830T ES 94925830 T ES94925830 T ES 94925830T ES 2119222 T3 ES2119222 T3 ES 2119222T3
Authority
ES
Spain
Prior art keywords
polypeptide
aqueous phases
polypeptides
isolation
phases
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94925830T
Other languages
English (en)
Inventor
Stuart Builder
Roger Hart
Philip Lester
John Ogez
David Reifsnyder
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genentech Inc
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Application granted granted Critical
Publication of ES2119222T3 publication Critical patent/ES2119222T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/803—Physical recovery methods, e.g. chromatography, grinding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/804—Single cell protein
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/808—Materials and products related to genetic engineering or hybrid or fused cell technology, e.g. hybridoma, monoclonal products

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Endocrinology (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Diabetes (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

SE DESCRIBE UN METODO PARA AISLAR UN POLIPEPTIDO EXOGENO EN UNA CONFORMACION NO NATIVA DE CELULAS, TAL COMO UN CALDO DE FERMENTACION ACUOSO, EN EL CUAL SE PREPARA, EL METODO COMPRENDE LA PUESTA EN CONTACTO DEL POLIPEPTIDO CON UN AGENTE CAOTROPICO Y PREFERIBLEMENTE UN AGENTE DE REDUCCION Y CON ESPECIES DE FORMACION DE FASE PARA FORMAR FASES ACUOSAS MULTIPLES, SIENDO ENRIQUECIDA UNA DE LAS FASES EN EL POLIPEPTIDO Y DEBILITADA EN SOLIDOS DE BIOMASA Y ACIDOS NUCLEICOS QUE SE ORIGINA DE LAS CELULAS. PREFERIBLEMENTE, EL METODO DA COMO RESULTADO DOS FASES ACUOSAS, CON UNA FASE SUPERIOR QUE ESTA ENRIQUECIDA EN EL POLIPEPTIDO.
ES94925830T 1993-08-20 1994-08-10 Aislamiento de polipeptidos en fases acuosas multiples. Expired - Lifetime ES2119222T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/110,663 US5407810A (en) 1993-08-20 1993-08-20 Aqueous multiple-phase isolation of polypeptide

Publications (1)

Publication Number Publication Date
ES2119222T3 true ES2119222T3 (es) 1998-10-01

Family

ID=22334217

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94925830T Expired - Lifetime ES2119222T3 (es) 1993-08-20 1994-08-10 Aislamiento de polipeptidos en fases acuosas multiples.

Country Status (11)

Country Link
US (3) US5407810A (es)
EP (1) EP0714403B1 (es)
JP (1) JP3646195B2 (es)
AT (1) ATE167193T1 (es)
AU (1) AU673624B2 (es)
CA (1) CA2167910C (es)
DE (1) DE69411015T2 (es)
DK (1) DK0714403T3 (es)
ES (1) ES2119222T3 (es)
GR (1) GR3027656T3 (es)
WO (1) WO1995006059A1 (es)

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TW517059B (en) * 1994-07-25 2003-01-11 Ciba Geigy Ag New process for the production of biologically active protein
WO1996004556A1 (en) * 1994-08-01 1996-02-15 Hsu James T Lipoprotein cholesterol assays
EP0779927A1 (en) * 1994-09-08 1997-06-25 Chiron Corporation A method of improved production of insulin-like growth factor
GB9618952D0 (en) 1996-09-11 1996-10-23 Sandoz Ltd Process
US6756484B1 (en) 1995-04-14 2004-06-29 Cephalon, Inc. IGF-I purification process
US5650496A (en) * 1995-04-14 1997-07-22 Cephalon, Inc. IGF-I purification process
US5789213A (en) * 1995-05-11 1998-08-04 Health Research Inc. Method and compositions for high efficiency loading, transfection and fusion of cells by electric pulses
DE69630524T2 (de) * 1995-06-07 2004-08-05 Chiron Corp., Emeryville Methoden zur aufreinigung von authentischem igf aus hefewirtszellen
DE69635051T2 (de) * 1995-06-07 2006-06-14 Chiron Corp Methode zur lösung, reinigung und rückfaltung von protein
US7193042B1 (en) 1995-06-07 2007-03-20 Chiron Corporation Methods for purifying authentic IGF from yeast hosts
US7071313B1 (en) 1995-06-07 2006-07-04 Cephalon, Inc. Methods of purifying authentic IGF from yeast hosts
US5945515A (en) * 1995-07-31 1999-08-31 Chomczynski; Piotr Product and process for isolating DNA, RNA and proteins
SE9504019D0 (sv) * 1995-11-13 1995-11-13 Pharmacia Ab Method for production of proteins
US5734024A (en) * 1996-04-19 1998-03-31 Boris Y. Zaslavsky Method for determining the biological activity of recombinant human growth hormone
IT1291362B1 (it) * 1997-05-13 1999-01-07 Vectorpharma Int Composizioni farmaceutiche multicomponente bifasiche contenenti sostanze atte a modificare la partizione dei principi attivi
WO2000010674A1 (en) * 1997-08-19 2000-03-02 Arnon Chait Method for evaluation of the ratio of amounts of biomolecules or their sub-populations in a mixture
US20020052026A1 (en) * 1997-10-08 2002-05-02 Steven M. Vicik Methods of refolding proteins
US6265542B1 (en) 1997-10-24 2001-07-24 Genentech, Inc. Purification of molecules
WO2000017353A1 (en) 1998-09-22 2000-03-30 Genentech, Inc. Ucp4
WO2000002996A2 (en) 1998-07-10 2000-01-20 Cornell Research Foundation, Inc. Recombinant constructs and systems for secretion of proteins via type iii secretion systems
DE69943058D1 (de) * 1998-11-30 2011-02-03 Genentech Inc Loslösendes protein 5 - ucp5
US7342102B2 (en) 1998-11-30 2008-03-11 Genentech, Inc. Uncoupling protein 5 (UCP5)
CA2358609A1 (en) * 1999-01-07 2000-07-13 Takeda Chemical Industries, Ltd. Process for producing activated protein
US6437101B1 (en) 1999-05-07 2002-08-20 Akzo Nobel N.V. Methods for protein purification using aqueous two-phase extraction
US7229789B1 (en) * 2001-01-19 2007-06-12 N.V. Organon Methods and compositions for extracting proteins from cells
HUP0500733A3 (en) 2001-02-09 2010-01-28 Genentech Inc Crystallization of igf-1
EP1314739A1 (en) * 2001-11-22 2003-05-28 Bayer Ag Process for renaturation of recombinant, disulfide containing proteins at high protein concentrations in the presence of amines
US7998705B2 (en) * 2002-08-06 2011-08-16 FUJIFILM Diosynth Biotechnologies U.S.A., Inc Increased dynamic binding capacity in ion exchange chromatography by addition of polyethylene glycol
US6924124B1 (en) * 2002-08-23 2005-08-02 Immunex Corporation Feeding strategies for cell culture
CA2526409A1 (en) * 2003-05-19 2004-12-02 Brandeis University Nucleic acid processing methods, kits and devices
US8415302B2 (en) 2004-01-28 2013-04-09 The Regents Of The University Of California Surgical applications for BMP binding protein
US8188219B2 (en) 2004-01-28 2012-05-29 The Regents Of The University Of California Bone morphogenic protein binding peptide
US8193312B2 (en) 2004-01-28 2012-06-05 The Regents Of The University Of California Bone morphogenic protein binding peptide
DE102004015965A1 (de) * 2004-04-01 2005-10-20 Aventis Pharma Gmbh Verfahren zur Gewinnung von Insulinen durch verbesserte Luftbegasung der Faltung
EP1761552B1 (en) 2004-06-29 2008-09-17 Ares Trading S.A. Process for the purification of il-18 binding protein
EP1853283B1 (en) * 2004-11-29 2015-09-16 The Regents of The University of California Activating extraction of demineralized bone matrix
CA2915270C (en) * 2005-08-05 2017-07-11 Amgen Inc. Stable aqueous protein or antibody pharmaceutical formulations and their preparation
US20070048786A1 (en) * 2005-09-01 2007-03-01 Arnon Chait Systems and methods for fractionation of protein mixtures
US9072709B2 (en) 2009-06-23 2015-07-07 The Regents Of The University Of California Enhancement of bone morphogenic protein (BMP) retention with BMP binding peptide (BBP)
KR20170060056A (ko) * 2014-09-26 2017-05-31 질레코 인코포레이티드 가용화된 효소 및 이의 용도
ES2893293T3 (es) * 2014-10-01 2022-02-08 Impossible Foods Inc Métodos de extracción y purificación de proteínas no desnaturalizadas
JP2016172707A (ja) * 2015-03-18 2016-09-29 株式会社ＵｎｉＢｉｏ 植物体内で発現させた上皮細胞増殖因子の精製品の製造方法
US11051532B2 (en) 2017-09-22 2021-07-06 Impossible Foods Inc. Methods for purifying protein
US12011016B2 (en) 2020-09-14 2024-06-18 Impossible Foods Inc. Protein methods and compositions

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Also Published As

Publication number Publication date
EP0714403A1 (en) 1996-06-05
DE69411015D1 (de) 1998-07-16
AU673624B2 (en) 1996-11-14
CA2167910A1 (en) 1995-03-02
CA2167910C (en) 2009-10-06
JP3646195B2 (ja) 2005-05-11
US5695958A (en) 1997-12-09
GR3027656T3 (en) 1998-11-30
ATE167193T1 (de) 1998-06-15
AU7561694A (en) 1995-03-21
DE69411015T2 (de) 1998-10-22
WO1995006059A1 (en) 1995-03-02
EP0714403B1 (en) 1998-06-10
US5407810A (en) 1995-04-18
US5723310A (en) 1998-03-03
JPH09501931A (ja) 1997-02-25
DK0714403T3 (da) 1999-03-22

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