ES2061720T3 - Transformacion de tejido vegetal. - Google Patents

Transformacion de tejido vegetal.

Info

Publication number
ES2061720T3
ES2061720T3 ES88810280T ES88810280T ES2061720T3 ES 2061720 T3 ES2061720 T3 ES 2061720T3 ES 88810280 T ES88810280 T ES 88810280T ES 88810280 T ES88810280 T ES 88810280T ES 2061720 T3 ES2061720 T3 ES 2061720T3
Authority
ES
Spain
Prior art keywords
transformation
plant tissue
vegetable material
corn
involves
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES88810280T
Other languages
English (en)
Inventor
Sharon Clarice Harvey Alfinito
Paul Shartzer Dietrich
Lynn Ellen Murry
Ralph Michael Sinibaldi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sandoz AG
Original Assignee
Sandoz AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sandoz AG filed Critical Sandoz AG
Application granted granted Critical
Publication of ES2061720T3 publication Critical patent/ES2061720T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal protein (delta-endotoxin)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Other Liquid Machine Or Engine Such As Wave Power Use (AREA)
  • Fertilizers (AREA)
  • Hydroponics (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Cultivation Of Plants (AREA)
  • Saccharide Compounds (AREA)
  • Steroid Compounds (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

LA INVENCION PROPORCIONA UN METODO PARA TRANSFORMAR MATERIAL VEGETAL QUE IMPLICA PONER EN CONTACTO EL MATERIAL CON UNA SOLUCION DE TRANSFORMACION QUE CONTIENE ADN Y UN AGENTE DE PERMEACION DE MEMBRANA, EN PRESENCIA DE UNA CORRIENTE ELECTRICA DUTRANTE UN TIEMPO SUFICIENTE PARA QUE SE REALICE LA TRANSFORMACION. TAMBIEN SE REFIERE LA INVENCION AL MATERIAL VEGETAL Y A LAS PLANTAS FERTILES ASI OBTENIDOS, ASI COMO A NUEVO MATERIAL VEGETAL TRANSFORMADO, EN PARTICULAR DE MAIZ.
ES88810280T 1987-05-05 1988-05-03 Transformacion de tejido vegetal. Expired - Lifetime ES2061720T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US2771287A 1987-05-05 1987-05-05

Publications (1)

Publication Number Publication Date
ES2061720T3 true ES2061720T3 (es) 1994-12-16

Family

ID=21839344

Family Applications (1)

Application Number Title Priority Date Filing Date
ES88810280T Expired - Lifetime ES2061720T3 (es) 1987-05-05 1988-05-03 Transformacion de tejido vegetal.

Country Status (18)

Country Link
EP (2) EP0290395B1 (es)
JP (1) JPS63301792A (es)
KR (1) KR880014104A (es)
CN (1) CN1030442A (es)
AT (1) ATE100858T1 (es)
AU (1) AU615905B2 (es)
BR (1) BR8802175A (es)
CA (1) CA1328236C (es)
DE (1) DE3887375T2 (es)
DK (1) DK240088A (es)
ES (1) ES2061720T3 (es)
HU (1) HUT46741A (es)
IE (2) IE940815L (es)
IL (1) IL86245A (es)
NZ (1) NZ224470A (es)
PL (1) PL272245A1 (es)
PT (1) PT87394B (es)
ZA (1) ZA883238B (es)

Families Citing this family (69)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TR27832A (tr) * 1987-04-29 1995-08-31 Monsanto Co Zararli ucucu hasarata mukavim bitkiler.
DE3856241T2 (de) * 1987-05-20 1999-03-25 Novartis Ag Verfahren zur Herstellug von transgene Zea mays-Pflanzen, die aus Protoplasten oder aus von Protopflasten erhaltenen Zellen regeneriert wurden
US5350689A (en) * 1987-05-20 1994-09-27 Ciba-Geigy Corporation Zea mays plants and transgenic Zea mays plants regenerated from protoplasts or protoplast-derived cells
JPH02500566A (ja) * 1987-08-17 1990-03-01 プラント・ジェネティック・システムズ・エヌ・ブイ バチルス・スリンギエンシスからのｄｎａ配列で形質転換された植物
EP0331083A3 (de) * 1988-03-02 1990-11-28 Schweizerische Eidgenossenschaft Eidgenössische Technische Hochschule (Eth) Verfahren zur Herstellung transgener Pflanzen
GB8806643D0 (en) * 1988-03-21 1988-04-20 Ici Plc Genetic manipulation
US5990387A (en) 1988-06-10 1999-11-23 Pioneer Hi-Bred International, Inc. Stable transformation of plant cells
FI902635A0 (fi) * 1989-05-29 1990-05-25 Eidgenoess Tech Hochschule Foerfarande foer framstaellning av transgena vaexter.
DK168302B1 (da) * 1989-06-29 1994-03-07 Danisco Fremgangsmåde til indføring af molekyler, især genetisk materiale i planteceller
US7705215B1 (en) 1990-04-17 2010-04-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5550318A (en) * 1990-04-17 1996-08-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US6803499B1 (en) 1989-08-09 2004-10-12 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
EP0814166A3 (en) * 1989-08-09 1998-05-13 DeKalb Genetics Corporation Methods and compositions for the production of stably transformed fertile monocot plants and cells thereof
US5484956A (en) * 1990-01-22 1996-01-16 Dekalb Genetics Corporation Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
US6329574B1 (en) 1990-01-22 2001-12-11 Dekalb Genetics Corporation High lysine fertile transgenic corn plants
HU220773B1 (hu) * 1990-01-22 2002-05-28 Dekalb Genetics Corporation Eljárás termő transzgenikus kukoricanövények előállítására
US6777589B1 (en) 1990-01-22 2004-08-17 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US6946587B1 (en) 1990-01-22 2005-09-20 Dekalb Genetics Corporation Method for preparing fertile transgenic corn plants
DE4013099A1 (de) * 1990-04-25 1991-10-31 Hoechst Ag Verfahren zur transformation somatischer embryonen von nutzpflanzen
EP0535060B1 (de) * 1990-06-15 1995-02-22 Hoechst Schering AgrEvo GmbH Antifungisches polypeptid, verfahren zu seiner herstellung
US6395966B1 (en) 1990-08-09 2002-05-28 Dekalb Genetics Corp. Fertile transgenic maize plants containing a gene encoding the pat protein
US6403865B1 (en) 1990-08-24 2002-06-11 Syngenta Investment Corp. Method of producing transgenic maize using direct transformation of commercially important genotypes
AU643563B2 (en) * 1990-11-01 1993-11-18 Sapporo Breweries Limited Method for preparing transformed plant
DK0558676T3 (da) * 1990-11-23 2000-09-25 Aventis Cropscience Nv Fremgangsmåde til transformering af enkimbladede planter
US5384253A (en) * 1990-12-28 1995-01-24 Dekalb Genetics Corporation Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes
UA48104C2 (uk) * 1991-10-04 2002-08-15 Новартіс Аг Фрагмент днк, який містить послідовність,що кодує інсектицидний протеїн, оптимізовану для кукурудзи,фрагмент днк, який забезпечує направлену бажану для серцевини стебла експресію зв'язаного з нею структурного гена в рослині, фрагмент днк, який забезпечує специфічну для пилку експресію зв`язаного з нею структурного гена в рослині, рекомбінантна молекула днк, спосіб одержання оптимізованої для кукурудзи кодуючої послідовності інсектицидного протеїну, спосіб захисту рослин кукурудзи щонайменше від однієї комахи-шкідника
US5780709A (en) * 1993-08-25 1998-07-14 Dekalb Genetics Corporation Transgenic maize with increased mannitol content
US6118047A (en) 1993-08-25 2000-09-12 Dekalb Genetic Corporation Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction
US5693506A (en) * 1993-11-16 1997-12-02 The Regents Of The University Of California Process for protein production in plants
US5750870A (en) * 1994-06-17 1998-05-12 Agritope, Inc. Plant genetic transformation methods and transgenic plants
HUT76841A (en) * 1994-07-29 1997-11-28 Pioneer Hi Bred Int Transgenic cereal plants
US5736369A (en) * 1994-07-29 1998-04-07 Pioneer Hi-Bred International, Inc. Method for producing transgenic cereal plants
CA2234656C (en) * 1995-10-13 2008-01-08 Dow Agrosciences Llc Modified bacillus thuringiensis gene for lepidopteran control in plants
US6713063B1 (en) 1996-11-20 2004-03-30 Monsanto Technology, Llc Broad-spectrum δ-endotoxins
US6017534A (en) 1996-11-20 2000-01-25 Ecogen, Inc. Hybrid Bacillus thuringiensis δ-endotoxins with novel broad-spectrum insecticidal activity
ES2229395T3 (es) 1996-11-20 2005-04-16 Monsanto Technology Llc Endotoxinas delta de amplio espectro.
US6121436A (en) 1996-12-13 2000-09-19 Monsanto Company Antifungal polypeptide and methods for controlling plant pathogenic fungi
US6040497A (en) * 1997-04-03 2000-03-21 Dekalb Genetics Corporation Glyphosate resistant maize lines
CA2888685C (en) 1997-04-03 2017-05-09 T. Michael Spencer Glyphosate resistant maize lines
AU2669599A (en) 1998-02-12 1999-08-30 Regents Of The University Of California, The Compositions for receptor/liposome mediated transfection and methods of using same
US6121521A (en) * 1998-04-01 2000-09-19 Novartis Ag Chimeric insecticidal protein and DNA coding therefor
GB0002814D0 (en) 2000-02-09 2000-03-29 Univ York Nucleic acids and their uses
AU2003254052B2 (en) 2002-07-19 2008-06-12 University Of South Carolina Compositions and methods for the modulation of gene expression in plants
ATE405658T1 (de) 2002-07-26 2008-09-15 Basf Plant Science Gmbh Neue selektionsverfahren
EP1502954A1 (en) 2003-07-31 2005-02-02 greenovation Biotech GmbH Utilisation of constructs comprising recombination sequence motifes for enhancing gene expression in moss
ES2346224T4 (es) 2003-11-10 2011-06-01 Icon Genetics Gmbh Sistema de expresión de planta derivado de virus de arn.
EP1616959A1 (en) 2004-07-07 2006-01-18 Icon Genetics AG Biological safe transient protein expression in plants
US8334430B2 (en) 2005-10-13 2012-12-18 Monsanto Technology Llc Methods for producing hybrid seed
US8153863B2 (en) 2007-03-23 2012-04-10 New York University Transgenic plants expressing GLK1 and CCA1 having increased nitrogen assimilation capacity
GB0709835D0 (en) 2007-05-22 2007-07-04 Plant Bioscience Ltd Composition and method for modulating plant root hair development
GB2465748B (en) 2008-11-25 2012-04-25 Algentech Sas Plant cell transformation method
GB2465749B (en) 2008-11-25 2013-05-08 Algentech Sas Plant cell transformation method
GB2467167B (en) 2009-01-26 2013-09-11 Algentech Sas Gene targeting in plants
EP2443241A1 (en) 2009-06-15 2012-04-25 Plant Bioscience Limited Production of viral capsids
BR112012002454A2 (pt) 2009-08-05 2017-07-04 Chromocell Corp plantas, micróbios e organismos melhorados
EP2357239A1 (en) 2009-10-29 2011-08-17 Universität zu Köln Methods and means for a selectable marker system in plants
GB201008720D0 (en) 2010-05-25 2010-07-07 Univ Aberdeen RxLR-leader peptides and protein translocation
US20130295609A1 (en) 2010-11-04 2013-11-07 Medicago Inc. Plant expression system
AP2013007316A0 (en) 2011-07-01 2013-12-31 Monsanto Technology Llc Methods and compositions for selective regulation of protein expression
WO2014147235A1 (en) 2013-03-22 2014-09-25 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Kits comprising plus-sense single stranded rna viral vectors and methods for producing polypeptides using the kits
ES2706499T3 (es) 2013-08-14 2019-03-29 Inst Of Genetics And Developmental Biology Métodos de modulación del tamaño de la semilla y de los órganos de plantas
GB201319876D0 (en) 2013-11-11 2013-12-25 Plant Bioscience Ltd Methods of modulating seed and organ size in plants
AU2015234595B2 (en) 2014-03-27 2021-03-25 Medicago Inc. Modified CPMV enhancer elements
RU2731295C2 (ru) 2014-07-11 2020-09-01 Медикаго Инк. Модификация продуцирования белков в растениях
ES2857741T3 (es) 2015-07-02 2021-09-29 Medicago Inc Activador de la vía del ácido jasmónico
EP3260542A1 (en) 2016-06-20 2017-12-27 Algentech Protein production in plant cells
WO2019185129A1 (en) 2018-03-27 2019-10-03 The University Court Of The University Of Glasgow Bacterial pathogen derived resistance in plants
EP4351320A1 (en) 2021-06-09 2024-04-17 ImmunityBio, Inc. Methods and systems for producing a protein of interest in a plant
WO2024074888A2 (en) 2022-10-03 2024-04-11 The Regents Of The University Of California Circumventing barriers to hybrid crops from genetically distant crosses

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1983001176A1 (en) * 1981-10-01 1983-04-14 Int Plant Research Inst Process for the genetic modification of cereals with transformation vectors
AU9054882A (en) * 1981-10-01 1983-04-27 International Plant Research Institute Process for the genetic modification of cerials with transformation vectors
BR8404834A (pt) * 1983-09-26 1985-08-13 Agrigenetics Res Ass Metodo para modificar geneticamente uma celula vegetal
CA1208146A (en) * 1983-10-27 1986-07-22 Tai-Kin Wong Method of transferring genes into cells
WO1985001856A1 (en) * 1983-11-03 1985-05-09 Johannes Martenis Jacob De Wet Method for the transfer of exogenous genes in plants using pollen as a vector
JPS63123325A (ja) * 1986-08-14 1988-05-27 マックス−プランク−ゲゼルシャフト・ツア・フェルデルング・デア・ヴィッセンシャフテン・エ−・ファウ トランスジェニック単子葉植物、その種子および植物の調製方法
IL84459A (en) * 1986-12-05 1993-07-08 Agracetus Apparatus and method for the injection of carrier particles carrying genetic material into living cells
TR27832A (tr) * 1987-04-29 1995-08-31 Monsanto Co Zararli ucucu hasarata mukavim bitkiler.

Also Published As

Publication number Publication date
DE3887375T2 (de) 1994-06-09
IL86245A0 (en) 1988-11-15
DK240088D0 (da) 1988-05-03
PT87394B (pt) 1992-09-30
NZ224470A (en) 1991-06-25
ATE100858T1 (de) 1994-02-15
PL272245A1 (en) 1989-02-06
DK240088A (da) 1988-11-06
IE881323L (en) 1988-11-05
KR880014104A (ko) 1988-12-22
CA1328236C (en) 1994-04-05
PT87394A (pt) 1989-05-31
EP0290395A3 (en) 1990-11-28
EP0290395A2 (en) 1988-11-09
IE63004B1 (en) 1995-03-08
JPS63301792A (ja) 1988-12-08
EP0290395B1 (en) 1994-01-26
AU615905B2 (en) 1991-10-17
DE3887375D1 (de) 1994-03-10
BR8802175A (pt) 1988-12-06
AU1550888A (en) 1988-11-10
ZA883238B (en) 1990-01-31
CN1030442A (zh) 1989-01-18
EP0574356A1 (en) 1993-12-15
IE940815L (en) 1988-11-05
HUT46741A (en) 1988-11-28
IL86245A (en) 1995-01-24

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