KR880014104A - 식물조직의 형질전환 방법 - Google Patents

식물조직의 형질전환 방법 Download PDF

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KR880014104A
KR880014104A KR1019880005222A KR880005222A KR880014104A KR 880014104 A KR880014104 A KR 880014104A KR 1019880005222 A KR1019880005222 A KR 1019880005222A KR 880005222 A KR880005222 A KR 880005222A KR 880014104 A KR880014104 A KR 880014104A
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plant
maize
dna
transformation
low susceptibility
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클레리스 하비 알피니토 샤론
샤트저 디에트리크 폴
엘른 머리 린
미첼 시니발디 랄프
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예안 클라메르 . 한스 루돌프 하우스
산도즈 리미티드
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

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  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
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Abstract

내용 없음

Description

식물조직의 형질전환 방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (20)

  1. 식물 재료(물질)내에 DNA의 운반 및 형질전환이 일어나기에 충분한 시간동안 전류의 준재하에서 DNA 및 막-투과제를 포함하는 형질전환 용액을 세포 재료와 접촉시키는 것을 포함하는 것을 특징으로 하는 식물세포 재료의 형질전환 방법.
  2. 제 1 항에 있어서, 상기 DNA는 벡터 DNA인 것을 특징으로 하는 방법.
  3. 제 2 항에 있어서, 상기 DNA는 플라스미드 DNA인 것을 특징으로 하는 형질전환 방법.
  4. 제 1 항 내지 제 3 항중 어느 한 항에 있어서, 형질전환시킬 식물세포 내에 수평 겔 전기영동시스템을 도입하고, DNA에서 식물세포로 전류가 흐르도록 DNA 물질을 웰에 담는 것을 특징으로 하는 형질전환방법.
  5. 제 4 항에 있어서, 상기 막-투과제는 디메틸 설폭시드, 리솔레시틴, 소디움 도데실 설페이트 및 다른 세제류로부터 선택된 극성 막-투과제인 것을 특징으로 하는 형질전환 방법.
  6. 제 1 항 내지 제 5 항중 어느 한 항에 있어서, 상기 식물 재료는 식물조직, 식물배아, 분열조직, 액아, 줄기대 또는 켈러스인 것을 특징으로 하는 형질전환 방법.
  7. 제 6 항에 있어서, 상기 식물 재료는 옥수수 식물 재료인 것을 특징으로 하는 형질전환 방법.
  8. 제 1 항 내지 제 7 항에 기술된 방법에 따라서 식물 세포를 형질전환시키고, 배양조건하에서 형질전환된 식물세포를 배양하는 것을 포함하는 것을 특징으로 하는 형질전환된 번식성 식물의 제조방법.
  9. 제 1 항 내지 제 8 항에 기재된 방법에 의해 얻어진 형질전환된 번식성 식물 및 이것의 씨앗(종자) 및 자손.
  10. 건강하고, 번식력있는 식물내에서 성장 또는 재생할 수 있는 숙주 또는 수용체종 이외의 다른 원천에서 발생한 식물 제놈 DNA를 포함하는 것을 특징으로 하는 단자엽 식물세포 재료.
  11. 제 10 항에 있어서, 식물세포 재료는 옥수수인 것을 특징으로 하는 식물세포 재료.
  12. 숙주 또는 수용체종 이외의 다른 원천에서 발생한 식물제놈 DNA를 포함하는 것을 특징으로 하는 건강하고, 번식력있는 단자엽식물.
  13. 숙주 또는 수용체종 이외의 다른 원천에서 발생한 식물제놈 DNA를 포함하는 것을 특징으로 하는 건강하고, 번식력있는 쌍자엽식물.
  14. 제 12 항 또는 제 13 항에 있어서, 상기 식물로 옥수수인 것을 특징으로 하는 식물.
  15. 제 14 항에 있어서, 상기 옥수수는 해충 또는 제초제에 대해 낮은 감수성을 가지는 것을 특징으로 하는 옥수수.
  16. 제 15 항에 있어서, 상기 옥수수는 해충에 대해 낮은 감수성을 가지는 것을 특징으로 하는 옥수수.
  17. 제 16 항에 있어서, 상기 옥수수는 곤충에 대해 낮은 감수성을 가지는 것을 특징으로 하는 옥수수.
  18. 제 17 항에 있어서, 상기 옥수수는 나비목(레피도프테라) 해충에 대해 낮은 감수성을 가지는 것을 특징으로 하는 옥수수.
  19. 제 17 항에 있어서, 상기 옥수수는 땅정벌레목(콜레오프테라)에 대해 낮은 감수성을 가지는 것을 특징으로 하는 옥수수.
  20. 제 11 항 내지 제 18 항 중의 어느 한 항에 따른 식물의 종자.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019880005222A 1987-05-05 1988-05-04 식물조직의 형질전환 방법 Withdrawn KR880014104A (ko)

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US2771287A 1987-05-05 1987-05-05
US027,712 1987-05-05

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EP (2) EP0574356A1 (ko)
JP (1) JPS63301792A (ko)
KR (1) KR880014104A (ko)
CN (1) CN1030442A (ko)
AT (1) ATE100858T1 (ko)
AU (1) AU615905B2 (ko)
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PT87394B (pt) 1992-09-30
ES2061720T3 (es) 1994-12-16
IL86245A0 (en) 1988-11-15
IE63004B1 (en) 1995-03-08
NZ224470A (en) 1991-06-25
CN1030442A (zh) 1989-01-18
AU1550888A (en) 1988-11-10
JPS63301792A (ja) 1988-12-08
DE3887375D1 (de) 1994-03-10
PT87394A (pt) 1989-05-31
AU615905B2 (en) 1991-10-17
ZA883238B (en) 1990-01-31
EP0574356A1 (en) 1993-12-15
IE940815L (en) 1988-11-05
EP0290395A2 (en) 1988-11-09
PL272245A1 (en) 1989-02-06
CA1328236C (en) 1994-04-05
DE3887375T2 (de) 1994-06-09
BR8802175A (pt) 1988-12-06
EP0290395A3 (en) 1990-11-28
ATE100858T1 (de) 1994-02-15
IL86245A (en) 1995-01-24
DK240088A (da) 1988-11-06
EP0290395B1 (en) 1994-01-26

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