BR0311362A - métodos, composições e fatores de crescimento e de diferenciação para produzir células produtoras de insulina - Google Patents

métodos, composições e fatores de crescimento e de diferenciação para produzir células produtoras de insulina

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Publication number
BR0311362A
BR0311362A BRPI0311362-0A BR0311362A BR0311362A BR 0311362 A BR0311362 A BR 0311362A BR 0311362 A BR0311362 A BR 0311362A BR 0311362 A BR0311362 A BR 0311362A
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BR
Brazil
Prior art keywords
cells
producing
growth
producing cells
differentiation factors
Prior art date
Application number
BRPI0311362-0A
Other languages
English (en)
Inventor
David Scharp
Sharon C Presnell
Mohammad Heidaran
Perry Haaland
Paul P Latta
Margaret Coutts
Catherine Mcintyre
Original Assignee
Novocell Inc
Becton Dickinson Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novocell Inc, Becton Dickinson Co filed Critical Novocell Inc
Publication of BR0311362A publication Critical patent/BR0311362A/pt

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    • C12N5/06—Animal cells or tissues; Human cells or tissues
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    • C12N5/0676—Pancreatic cells
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Abstract

"MéTODOS, COMPOSIçõES E FATORES DE CRESCIMENTO E DE DIFERENCIAçãO PARA PRODUZIR CéLULAS PRODUTORAS DE INSULINA". A presente invenção refere-se a um método para converter células pancreáticas diferenciadas não produtoras de hormónio em células diferenciadas produtoras de hormónio. o método compreende duas etapas: primeiro, cultivar células sob condições que convertem células diferenciadas não produtoras de hormónio em células tronco, e, segundo, cultivar células tronco sob condições que proporcionam diferenciar células tronco em células produtoras de hormónio. A invenção define os fatores de crescimento e diferenciação que são apresentados às células tronco para resultar em sua diferenciação nas células produtoras de hormónio, especialmente células produtoras de insulina. A invenção oferece uma nova fonte de grandes quantidades de células produtoras de hormónio, tais como células produtoras de insulina, as quais não estão correntemente disponiveis para usos terapêuticos, tal como o tratamento de diabetes.
BRPI0311362-0A 2002-05-28 2003-05-28 métodos, composições e fatores de crescimento e de diferenciação para produzir células produtoras de insulina BR0311362A (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US38400002P 2002-05-28 2002-05-28
US44373303A 2003-05-22 2003-05-22
PCT/US2003/016734 WO2003100026A2 (en) 2002-05-28 2003-05-28 Methods, compositions, and growth and differentiation factors for insulin-producing cells

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BR0311362A true BR0311362A (pt) 2006-10-31

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EP (1) EP1507552A2 (pt)
JP (1) JP2006506047A (pt)
CN (1) CN1668324A (pt)
AU (1) AU2003240810A1 (pt)
BR (1) BR0311362A (pt)
CA (1) CA2487858A1 (pt)
WO (1) WO2003100026A2 (pt)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1572984T3 (en) 2002-12-16 2016-06-13 Technion Res & Dev Foundation FEEDER CELL-FREE, XENOPHRIC CULTIVATION SYSTEM FOR HUMAN EMBRYONAL STEM CELLS
US8647873B2 (en) 2004-04-27 2014-02-11 Viacyte, Inc. PDX1 expressing endoderm
US7541185B2 (en) 2003-12-23 2009-06-02 Cythera, Inc. Methods for identifying factors for differentiating definitive endoderm
US7985585B2 (en) 2004-07-09 2011-07-26 Viacyte, Inc. Preprimitive streak and mesendoderm cells
DK1709159T3 (da) 2003-12-23 2019-07-29 Viacyte Inc Definitiv endoderm
US7625753B2 (en) 2003-12-23 2009-12-01 Cythera, Inc. Expansion of definitive endoderm cells
US8586357B2 (en) 2003-12-23 2013-11-19 Viacyte, Inc. Markers of definitive endoderm
US20050266554A1 (en) 2004-04-27 2005-12-01 D Amour Kevin A PDX1 expressing endoderm
EP2377922B1 (en) * 2004-04-27 2020-04-08 Viacyte, Inc. PDX1 expressing endoderm
JPWO2006001471A1 (ja) * 2004-06-29 2008-04-17 国立大学法人 東京大学 グルコース応答性インスリン分泌を促進するための医薬組成物
EP1786896B1 (en) * 2004-07-09 2018-01-10 Viacyte, Inc. Methods for identifying factors for differentiating definitive endoderm
US8187878B2 (en) 2004-08-13 2012-05-29 University Of Georgia Research Foundation, Inc. Methods for increasing definitive endoderm differentiation of pluripotent human embryonic stem cells with PI-3 kinase inhibitors
US8039254B2 (en) 2004-11-22 2011-10-18 Ramot At Tel-Aviv University Ltd. Populations of expanded and re-differentiated adult islet beta cells capable of producing insulin and methods of generating same
JP2008528038A (ja) * 2005-01-31 2008-07-31 エス セル インターナショナル ピーティーイー リミテッド 胚性幹細胞の指示された分化及びその利用
JP4534039B2 (ja) * 2005-04-12 2010-09-01 国立大学法人群馬大学 糖代謝促進剤並びに肥満及び糖尿病治療薬のスクリーニング方法
WO2006108651A2 (en) * 2005-04-12 2006-10-19 Develogen Aktiengesellschaft Use of activin products for preventing and treating diabetes and/or metabolic syndrome
JP5584875B2 (ja) * 2005-04-13 2014-09-10 国立大学法人 千葉大学 成体膵由来膵幹細胞の製造方法
DK2674485T3 (da) 2005-10-27 2019-08-26 Viacyte Inc Pdx-1 udtrykkende dorsal og ventral fortarm endoderm
EP2650360B1 (en) 2006-03-02 2019-07-24 Viacyte, Inc. Endocrine precursor cells, pancreatic hormone-expressing cells and methods of production
US11254916B2 (en) 2006-03-02 2022-02-22 Viacyte, Inc. Methods of making and using PDX1-positive pancreatic endoderm cells
US7695965B2 (en) 2006-03-02 2010-04-13 Cythera, Inc. Methods of producing pancreatic hormones
US20080044848A1 (en) * 2006-06-09 2008-02-21 Heidaran Mohammad A Placental niche and use thereof to culture stem cells
US7695963B2 (en) 2007-09-24 2010-04-13 Cythera, Inc. Methods for increasing definitive endoderm production
EP2356227B1 (en) 2008-11-14 2018-03-28 Viacyte, Inc. Encapsulation of pancreatic cells derived from human pluripotent stem cells
US9328331B2 (en) * 2009-05-21 2016-05-03 Joslin Diabetes Center, Inc. Compositions and methods for promoting beta cell maturity
EP2281874A1 (en) * 2009-07-31 2011-02-09 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Production of ß-Cells
WO2011071085A1 (ja) * 2009-12-08 2011-06-16 国立大学法人東京大学 多能性幹細胞から分化誘導された細胞の生産方法
WO2011081222A1 (ja) 2009-12-29 2011-07-07 武田薬品工業株式会社 膵ホルモン産生細胞の製造法
WO2011110722A1 (es) * 2010-03-12 2011-09-15 Fundación Progreso Y Salud Método para la proliferación "in vitro" de células procedentes de tejidos de origen endodérmico
CN103429739B (zh) 2010-05-12 2018-11-13 哥伦比亚大学纽约管理委员会 制备产生和分泌胰岛素的肠内分泌细胞的方法
US9404087B2 (en) 2010-12-15 2016-08-02 Kadimastem Ltd. Insulin producing cells derived from pluripotent stem cells
WO2012122318A2 (en) * 2011-03-07 2012-09-13 Massachusetts Institute Of Technology Methods for transfecting cells with nucleic acids
MX2015017103A (es) 2013-06-11 2016-11-07 Harvard College Celulas beta derivadas de células madre ( sc-beta ) y composiciones y métodos para generarlas.
DK2840132T3 (en) 2013-08-22 2017-02-20 Georg-August-Universität Göttingen Stiftung Öffenlichen Rechts Universitätsmedizin Method of Manufacturing Constructed Heart Muscle (EHM)
US9404086B2 (en) * 2014-03-26 2016-08-02 Ge Healthcare Uk Limited Method for cell differentiation
EP3160503B1 (en) 2014-06-26 2021-02-17 The Trustees of Columbia University in the City of New York Inhibition of serotonin expression in gut enteroendocrine cells results in conversion to insulin-positive cells
US10443042B2 (en) 2014-12-18 2019-10-15 President And Fellows Of Harvard College Serum-free in vitro directed differentiation protocol for generating stem cell-derived beta cells and uses thereof
WO2016100930A1 (en) 2014-12-18 2016-06-23 President And Fellows Of Harvard College Methods for generating stem cell-derived b cells and methods of use thereof
US10190096B2 (en) 2014-12-18 2019-01-29 President And Fellows Of Harvard College Methods for generating stem cell-derived β cells and uses thereof
IT201700066486A1 (it) * 2017-06-15 2018-12-15 Azienda Ospedaliero Univ Policlinico S Orsola Malpighi Bologn A Siero sanguigno per uso nel trattamento di patologie neurodegenerative oftalmologiche
EP3656852A4 (en) * 2017-07-20 2021-04-21 Riken RETINAL TISSUE MATURATION PROCESS CONTAINING A CONTINUOUS EPITHELIUM
BR112020009275A2 (pt) 2017-11-15 2020-10-27 Semma Therapeutics, Inc. composições de fabricação de célula de ilhota e métodos de uso
EP3741844A4 (en) * 2018-01-16 2021-09-15 Keio University METHOD OF DERIVING CORNEAL DOTHELIAN REPLACEMENT CELLS FROM IPS CELLS
EP3833365A4 (en) 2018-08-10 2022-05-11 Vertex Pharmaceuticals Incorporated ISLE DIFFERENTIATION DERIVED FROM STEM CELLS
TWI776368B (zh) * 2020-05-29 2022-09-01 國璽幹細胞應用技術股份有限公司 細胞分化培養基組合物、高分泌量胰島素產生細胞及其製備方法
CN115433684A (zh) * 2022-10-20 2022-12-06 云南师范大学 一种盘基网柄菌细胞同步化方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5935852A (en) * 1997-07-03 1999-08-10 Genetics Institute, Inc. DNA molecules encoding mammalian cerberus-like proteins

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