WO2003100026A3 - Methods, compositions, and growth and differentiation factors for insulin-producing cells - Google Patents

Methods, compositions, and growth and differentiation factors for insulin-producing cells Download PDF

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Publication number
WO2003100026A3
WO2003100026A3 PCT/US2003/016734 US0316734W WO03100026A3 WO 2003100026 A3 WO2003100026 A3 WO 2003100026A3 US 0316734 W US0316734 W US 0316734W WO 03100026 A3 WO03100026 A3 WO 03100026A3
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WO
WIPO (PCT)
Prior art keywords
cells
producing cells
hormone
insulin
producing
Prior art date
Application number
PCT/US2003/016734
Other languages
French (fr)
Other versions
WO2003100026A2 (en
Inventor
David Scharp
Original Assignee
Novocell Inc
Becton Dickinson Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novocell Inc, Becton Dickinson Co filed Critical Novocell Inc
Priority to BRPI0311362-0A priority Critical patent/BR0311362A/en
Priority to JP2004508268A priority patent/JP2006506047A/en
Priority to AU2003240810A priority patent/AU2003240810A1/en
Priority to CA002487858A priority patent/CA2487858A1/en
Priority to EP03731406A priority patent/EP1507552A2/en
Publication of WO2003100026A2 publication Critical patent/WO2003100026A2/en
Publication of WO2003100026A3 publication Critical patent/WO2003100026A3/en

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    • C12N5/06—Animal cells or tissues; Human cells or tissues
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    • C12N5/0676—Pancreatic cells
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    • C12N2506/22—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from pancreatic cells

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Abstract

A method of converting differentiated non-hormone producing pancreatic cells into differentiated hormone producing cells is disclosed. The method comprises two steps: first, culturing cells under conditions which convert differentiated non-hormone producing cells into stem cells; and second, culturing stem cells under conditions which provide for differentiating stem cells into hormone-producing cells. The invention defines growth and differentiation factors that are presented to the stem cells to result in their differentiation into hormone-producing cells, especially insulin-producing cells. The invention provides a new source of large quantities of hormone producing cells such as insulin-producing cells that are not currently available for therapeutic uses such as the treatment of diabetes.
PCT/US2003/016734 2002-05-28 2003-05-28 Methods, compositions, and growth and differentiation factors for insulin-producing cells WO2003100026A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
BRPI0311362-0A BR0311362A (en) 2002-05-28 2003-05-28 methods, compositions and growth and differentiation factors for producing insulin producing cells
JP2004508268A JP2006506047A (en) 2002-05-28 2003-05-28 Methods, components and growth and differentiation factors for insulin producing cells
AU2003240810A AU2003240810A1 (en) 2002-05-28 2003-05-28 Methods, compositions, and growth and differentiation factors for insulin-producing cells
CA002487858A CA2487858A1 (en) 2002-05-28 2003-05-28 Methods, compositions, and growth and differentiation factors for insulin-producing cells
EP03731406A EP1507552A2 (en) 2002-05-28 2003-05-28 Methods, compositions, and growth and differentiation factors for insulin-producing cells

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US38400002P 2002-05-28 2002-05-28
US60/384,000 2002-05-28
US44373303A 2003-05-22 2003-05-22
US10/443,733 2003-05-22

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WO2003100026A2 WO2003100026A2 (en) 2003-12-04
WO2003100026A3 true WO2003100026A3 (en) 2004-11-04

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PCT/US2003/016734 WO2003100026A2 (en) 2002-05-28 2003-05-28 Methods, compositions, and growth and differentiation factors for insulin-producing cells

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EP (1) EP1507552A2 (en)
JP (1) JP2006506047A (en)
CN (1) CN1668324A (en)
AU (1) AU2003240810A1 (en)
BR (1) BR0311362A (en)
CA (1) CA2487858A1 (en)
WO (1) WO2003100026A2 (en)

Cited By (1)

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Publication number Priority date Publication date Assignee Title
US9132226B2 (en) 2008-11-14 2015-09-15 Viacyte, Inc. Encapsulation of pancreatic cells derived from human pluripotent stem cells

Families Citing this family (45)

* Cited by examiner, † Cited by third party
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SI1572984T1 (en) * 2002-12-16 2016-07-29 Technion Research & Development Foundation Ltd. Feeder-free, xeno-free culture system for human embryonic stem cells
US7541185B2 (en) 2003-12-23 2009-06-02 Cythera, Inc. Methods for identifying factors for differentiating definitive endoderm
US8647873B2 (en) 2004-04-27 2014-02-11 Viacyte, Inc. PDX1 expressing endoderm
US7985585B2 (en) 2004-07-09 2011-07-26 Viacyte, Inc. Preprimitive streak and mesendoderm cells
MX2009009225A (en) 2003-12-23 2009-09-28 Cythera Inc Definitive endoderm.
US20050266554A1 (en) 2004-04-27 2005-12-01 D Amour Kevin A PDX1 expressing endoderm
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