WO2002040630A3 - Process of decellularizing biological matrices and acellular biological matrices useful in tissue engineering - Google Patents

Process of decellularizing biological matrices and acellular biological matrices useful in tissue engineering Download PDF

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Publication number
WO2002040630A3
WO2002040630A3 PCT/IL2001/001060 IL0101060W WO0240630A3 WO 2002040630 A3 WO2002040630 A3 WO 2002040630A3 IL 0101060 W IL0101060 W IL 0101060W WO 0240630 A3 WO0240630 A3 WO 0240630A3
Authority
WO
WIPO (PCT)
Prior art keywords
biological matrices
acellular
decellularizing
biological matrix
tissue engineering
Prior art date
Application number
PCT/IL2001/001060
Other languages
French (fr)
Other versions
WO2002040630A2 (en
Inventor
Gilad E Amiel
Original Assignee
Gilad E Amiel
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gilad E Amiel filed Critical Gilad E Amiel
Priority to AU2002224003A priority Critical patent/AU2002224003A1/en
Publication of WO2002040630A2 publication Critical patent/WO2002040630A2/en
Publication of WO2002040630A3 publication Critical patent/WO2002040630A3/en

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
    • A61L27/3691—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by physical conditions of the treatment, e.g. applying a compressive force to the composition, pressure cycles, ultrasonic/sonication or microwave treatment, lyophilisation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
    • A61L27/3687—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by the use of chemical agents in the treatment, e.g. specific enzymes, detergents, capping agents, crosslinkers, anticalcification agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/40—Preparation and treatment of biological tissue for implantation, e.g. decellularisation, cross-linking

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Botany (AREA)
  • Dermatology (AREA)
  • Medicinal Chemistry (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Transplantation (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Zoology (AREA)
  • Peptides Or Proteins (AREA)
  • Materials For Medical Uses (AREA)
  • Prostheses (AREA)

Abstract

A method of decellularizing a biological matrix so as to obtain an acellular biological matrix, as well as clinical uses of the acellular biological matrix are disclosed. The method is effected by (a) obtaining a tissue of interest; (b) treating the tissue of interest with a decellularization solution containing a cell lysis reagent, preferably a combination of a non-ionic detergent with a mild base; (c) removing cell remnants, thereby obtaining the acellular biological matrix; and optionally (d) dehydrating the acellular biological matrix.
PCT/IL2001/001060 2000-11-15 2001-11-15 Process of decellularizing biological matrices and acellular biological matrices useful in tissue engineering WO2002040630A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002224003A AU2002224003A1 (en) 2000-11-15 2001-11-15 Process of decellularizing biological matrices and acellular biological matrices useful in tissue engineering

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IL13970800A IL139708A0 (en) 2000-11-15 2000-11-15 Process of decellularizing biological matrices and acellular biological matrices useful in tissue engineering
IL139708 2000-11-15

Publications (2)

Publication Number Publication Date
WO2002040630A2 WO2002040630A2 (en) 2002-05-23
WO2002040630A3 true WO2002040630A3 (en) 2004-06-10

Family

ID=11074822

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IL2001/001060 WO2002040630A2 (en) 2000-11-15 2001-11-15 Process of decellularizing biological matrices and acellular biological matrices useful in tissue engineering

Country Status (3)

Country Link
AU (1) AU2002224003A1 (en)
IL (1) IL139708A0 (en)
WO (1) WO2002040630A2 (en)

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KR101595108B1 (en) 2006-10-06 2016-02-18 안트로제네시스 코포레이션 Nativetelopeptide Placental Collagen Compositions

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JP4092397B2 (en) * 2002-09-10 2008-05-28 国立循環器病センター総長 Treatment of living tissue for transplantation by applying ultrahigh hydrostatic pressure
US7459154B2 (en) * 2002-12-26 2008-12-02 Cell Genesys, Inc. Methods and reagents for the enhancement of virus transduction in the bladder epithelium
GB2423934B (en) 2003-09-04 2007-11-28 Cook Biotech Inc Extracellular matrix composite materials, and manufacture and use thereof
AU2005323327B2 (en) * 2005-01-05 2009-11-05 The Cleveland Clinic Foundation Method for fixing tissue
EP1863545B1 (en) 2005-03-19 2015-11-18 Cook Biotech, Inc. Prosthetic implants including ECM composite material
US9493735B2 (en) 2005-04-15 2016-11-15 Wake Forest University Health Sciences Bioreactor system and method of enhancing functionality of muscle cultured in vitro
EP1719533A1 (en) * 2005-05-04 2006-11-08 Tecnobiomedica S.p.A. Method of preparing acellularized, biocompatible, implantable material
US8568761B2 (en) * 2005-07-15 2013-10-29 Cormatrix Cardiovascular, Inc. Compositions for regenerating defective or absent myocardium
US9072816B2 (en) * 2006-01-18 2015-07-07 Cormatrix Cardiovascular, Inc. Composition for modulating inflammation of cardiovascular tissue
SG170731A1 (en) 2005-08-26 2011-05-30 Univ Minnesota Decellularization and recellularization of organs and tissues
EP1965812A4 (en) * 2005-11-08 2010-02-24 Georgia Tech Res Inst Acellularized biomaterial from embryonic stem cells
US20160082153A1 (en) * 2006-01-18 2016-03-24 Cormatrix Cardiovascular, Inc. Method and System for Treatment of Damaged Biological Tissue
GB0606231D0 (en) 2006-03-29 2006-05-10 Univ Leeds Improvements relating to decellurisation of tissue matrices for bladder implantation
AU2013203211B2 (en) * 2006-10-06 2015-05-28 Celularity Inc. Native (telopeptide) placental collagen compositions
CA2667214C (en) 2006-10-23 2015-12-01 Cook Biotech Incorporated Processed ecm materials with enhanced component profiles
CZ301086B6 (en) * 2007-10-17 2009-11-04 Bio-Skin, A. S. Sterile autogenous, allogenic or xenogenic implant and process for preparing thereof
WO2010019753A2 (en) * 2008-08-14 2010-02-18 Kci Licensing, Inc. Tissue scaffolds
WO2012005760A1 (en) * 2010-06-30 2012-01-12 Miromatrix Medical Inc. Use of perfusion decellularized organs for matched recellularization
EP2611472B1 (en) 2010-09-01 2016-02-10 Regents of the University of Minnesota Methods of recellularizing a tissue or organ for improved transplantability
ES2724587T3 (en) 2011-01-06 2019-09-12 Humacyte Inc Constructs generated by tissue engineering
US20130013083A1 (en) * 2011-01-06 2013-01-10 Humacyte Tissue-Engineered Constructs
AU2012262311B2 (en) 2011-05-31 2016-04-28 Lifecell Corporation Adipose tissue matrices
CN104053992B (en) * 2012-01-16 2016-12-14 皇家飞利浦有限公司 Determine the existence of target molecule in the body fluid including cell
ES2716993T3 (en) 2012-03-08 2019-06-18 Lifecell Corp Matrices of collagen and tissue activated by enzymes
US9290738B2 (en) 2012-06-13 2016-03-22 Miromatrix Medical Inc. Methods of decellularizing bone
ES2676043T3 (en) 2012-07-05 2018-07-16 Lifecell Corporation Tissue Drainage Collectors
CN103536967B (en) * 2012-07-10 2016-04-13 上海微创医疗器械（集团）有限公司 A kind of method for removing cells preparing extracellular matrix support material
BR112015000547B1 (en) 2012-07-13 2020-11-10 Lifecell Corporation method for treating tissue
AU2013323747B2 (en) 2012-09-26 2017-02-02 Lifecell Corporation Processed adipose tissue
US10035980B2 (en) 2013-01-14 2018-07-31 Florida State University Research Foundation, Inc. Extracellular matrix derived from stem cells and methods for production
BR112015023642A2 (en) 2013-03-15 2017-07-18 Miromatrix Medical Inc use of decellularized liver perfusion to allow cell recellularization
CN104353115B (en) * 2014-10-29 2016-06-01 南通大学附属医院 Take off the test kit of cytoskeleton, the preparation of support for pancreas and plant method for planting again
CN105013014A (en) * 2015-07-31 2015-11-04 武汉蓝普医品有限公司 Preparation method and application of acellular matrix biological material
CN105920670A (en) * 2016-05-19 2016-09-07 李世荣 Degradable male genital organ patch composite material with biological elasticity and preparation method thereof
CN109715140B (en) 2016-06-15 2022-06-24 通用医疗公司 Metabolic labeling and molecular enhancement of biological materials using bio-orthogonal reactions
CA3029579A1 (en) 2016-07-05 2018-01-11 Lifecell Corporation Tissue matrices incorporating multiple tissue types
WO2018048899A1 (en) 2016-09-06 2018-03-15 Micromatrix Medical Inc. Use of resected liver serum for whole liver engineering
EP3632481B1 (en) * 2017-05-30 2023-08-23 Adeka Corporation Method for producing decellularized material for transplantation and graft composition comprising biocompatible material including said material
CN107596446B (en) * 2017-09-16 2020-07-28 温州医科大学 Decellularization kit for maintaining tissue ultrastructure and nutrition microenvironment
AU2018351051A1 (en) 2017-10-18 2020-03-19 Lifecell Corporation Adipose tissue products and methods of production
US11123375B2 (en) 2017-10-18 2021-09-21 Lifecell Corporation Methods of treating tissue voids following removal of implantable infusion ports using adipose tissue products
US11246994B2 (en) 2017-10-19 2022-02-15 Lifecell Corporation Methods for introduction of flowable acellular tissue matrix products into a hand
ES2960617T3 (en) 2017-10-19 2024-03-05 Lifecell Corp Fluid Acellular Tissue Matrix Products and Production Methods
CN110876816A (en) * 2018-09-06 2020-03-13 韩嫣 Pig bladder source bioactive repair material
BR112021024043A2 (en) 2019-05-30 2022-02-08 Lifecell Corp biological breast implant
CN111569151B (en) * 2020-05-12 2021-12-17 上海亚朋生物技术有限公司 Acellular dermal matrix tissue engineering scaffold and preparation method thereof

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Cited By (2)

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Publication number Priority date Publication date Assignee Title
KR101595108B1 (en) 2006-10-06 2016-02-18 안트로제네시스 코포레이션 Nativetelopeptide Placental Collagen Compositions
KR101644985B1 (en) 2006-10-06 2016-08-02 안트로제네시스 코포레이션 Native(telopeptide) Placental Collagen Compositions

Also Published As

Publication number Publication date
WO2002040630A2 (en) 2002-05-23
AU2002224003A1 (en) 2002-05-27
IL139708A0 (en) 2002-02-10

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