CN105920670A - Degradable male genital organ patch composite material with biological elasticity and preparation method thereof - Google Patents
Degradable male genital organ patch composite material with biological elasticity and preparation method thereof Download PDFInfo
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- CN105920670A CN105920670A CN201610338058.6A CN201610338058A CN105920670A CN 105920670 A CN105920670 A CN 105920670A CN 201610338058 A CN201610338058 A CN 201610338058A CN 105920670 A CN105920670 A CN 105920670A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
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- A—HUMAN NECESSITIES
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- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
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- A—HUMAN NECESSITIES
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
- A61L27/362—Skin, e.g. dermal papillae
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
- A61L27/3629—Intestinal tissue, e.g. small intestinal submucosa
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
- A61L27/3687—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by the use of chemical agents in the treatment, e.g. specific enzymes, detergents, capping agents, crosslinkers, anticalcification agents
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/58—Materials at least partially resorbable by the body
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- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
- A61B2017/00831—Material properties
- A61B2017/00862—Material properties elastic or resilient
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Abstract
The invention discloses a degradable male genital organ patch composite material with the biological elasticity and a preparation method thereof. The patch composite material is prepared from biomembrane substrate powder and a biodegradable high-molecular polymer according to the mass ratio of 4:1-1:4 and obtained through the steps that the biomembrane substrate powder and the biodegradable high-molecular polymer are dissolved into a solvent to prepare a solution, and the solution is poured into a mold to be molded and then taken out of the mold to be subjected to freeze drying, wherein the biomembrane substrate powder is powder prepared through the step that a biomembrane is degreased, decellularized and subjected to antigen removal. The composite material is good in mechanical property, easy to bend and capable of being metabolized. A natural biomembrane is adopted as a biomembrane substrate, and the biomembrane substrate has the good elasticity, biocompatibility and bioactivity; the adopted high-molecular polymer material is biodegradable high molecules and has the excellent slow degradation advantage.
Description
Technical field
The invention belongs to field of material technology, be specifically related to the male genital organ sticking patch composite wood of a kind of degradable tool bioelastic
Material and preparation method thereof.
Background technology
In recent years, along with the development of society, growth in the living standard, more and more male wish to be allowed by male genetic shaping
Oneself become more self-confident, but after traditional genitals packing material such as silica gel, autologous fat etc. is filled, metabolism is very fast, also can go out
Existing silica gel shifts, has the situation such as foreign body sensation, autologous fat out-of-flatness, allows numerous patients and doctor all feel blemish in an otherwise perfect thing.
Summary of the invention
In view of this, it is an object of the invention to provide the male genital organ sticking patch composite of a kind of degradable tool bioelastic
And preparation method thereof.The male genital organ sticking patch composite shapes that the present invention provides, for having circular cylindrical form, is more suitable for hands
Art uses；And this composite possesses good biocompatibility, biological activity and elasticity, degraded is slowly.
The technical scheme that the present invention takes is as follows:
1, the male genital organ sticking patch composite of a kind of degradable tool bioelastic, can be dropped with biology by biofilm matrix powder
Solve high molecular polymer in mass ratio 4～1:1～4 composition, biofilm matrix powder is dissolved with Biodegradable high-molecular polymer
Pour die for molding into after being prepared as solution in solvent, be then removed from the molds and lyophilization obtains；Described biomembrane base
Matter powder be biomembrane through defat, de-cell, except antigen after the powder made.
Preferably biofilm matrix powder and Biodegradable high-molecular polymer 4:1,3:1,2.5:1,2:1,1:1,1:2 in mass ratio,
1:2.5,1:3,1:4 form.
It should be noted that described mould can use routine techniques means to be designed according to the shape of required composite.
Preferably, described Biodegradable high-molecular polymer is pla-pcl, polylactic acid, polyglycolic acid, gathers dioxocyclohex
Polymer after ketone, poly (glycolide-lactide) or their modifications by copolymerization.
Preferably, described biomembrane is the submucous layer of small intestine of animal, mucous membrane of urinary bladder, pericardium, skin or the animal limb tendons.
Preferably, described include that the biomembrane after defat is taken off cell uses pepsin enzymolysis after being beaten into liquid except the step of antigen,
The step saltoutd.
With ferment treatment, the terminal peptide of collagen fibrous proteins can be hydrolyzed, improve the productivity of collagen protein；But also will not destroy
The triple helix structure of collagen protein, keeps its characteristic.After enzymolysis by saltouing, centrifugal by the way of separate acquisition biomembrane
Base portion.
Preferably, described solvent is hexafluoroisopropanol, chloroform, TEG, carbon tetrachloride or ethyl acetate.
Preferably, described mould is composite can be fixed to helical form or axially penetrate through the mould of column of an opening.
2, the preparation method of the male genital organ sticking patch composite of a kind of degradable tool bioelastic, comprises the steps:
1) biomembrane is produced；
2) defat: by step 1) obtain biomembrane be placed in soak degreasing 12h in the methanol-chloroform mixed liquor of volume ratio 1:1, go
Ionized water rinses 3 times；
3) de-cell: by step 2) material after defat is placed in the normal saline containing pancreatin and EDTA, and 4 DEG C of vibrations disappear
Changing 48h, ionized water cleans 3 times repeatedly；The weight/mass percentage composition of described pancreatin and EDTA is 0.05%；
4) soak and pull an oar: by step 3) process after material be cut in the glacial acetic acid solution adding volume fraction 0.5% after segment
4 DEG C are soaked 12h, making beating, add the glacial acetic acid solution of volume fraction 0.5% in pulping process, it is to avoid heating causes protein denaturation；
5) enzymolysis, saltout: the liquid after making beating is added enzymolysis 48h under the conditions of pepsin 4 DEG C, filters after enzymolysis and obtain
Obtain filtrate；Filtrate is put into 4 DEG C of 12h that saltout in the NaCl solution of 2mol/L, and under the conditions of 4 DEG C, 8000rpm is centrifuged 45min,
Obtain solids；
6) prepare powder: by step 5) centrifugal after the solids that obtains molten with the glacial acetic acid of volume fraction 0.5% under the conditions of 4 DEG C
Liquid dissolves, dialysis, and powder is made in lyophilizing；
7) composite is prepared: with solvent by Biodegradable high-molecular polymer and step 6) powder that obtains dissolves, pours into
In mould, mould is immersed in the water 2h molding；Material after molding is taken out and is placed in-20 DEG C of freezing 4h ,-95 DEG C of negative pressure lyophilizing,
Negative pressure is kept to obtain the male genital organ sticking patch composite of degradable tool bioelastic after 0.2hPa (hundred handkerchiefs) following 48h.
Preferably, described step 5) described in enzymolysis time pepsic consumption take off in terms of the biofilm weight after cell by defat, often
Gram it is 4000U by enzyme amount.
Preferably, described step 6) described in dialyse the bag filter molecular cut off 7000D used, the dialysis solution of use is for going
Ionized water.
Preferably, described step 6) described in lyophilizing first freezing shape 12h, then-95 DEG C of negative pressure lyophilizing at-20 DEG C, keep
Negative pressure is 48h below 0.2 hundred handkerchiefs.
It should be noted that biomembranous acquisition includes biomembranous stripping, goes the operations such as fat, obtaining step is this area
Conventional mode of operation.
It is conventional method that solvent dissolves the method for Biodegradable high-molecular polymer, as a example by polycaprolactone, and can be by it with poly-
Ether F127 dissolves in the mode of 90-160 DEG C of mixing.
Described Biodegradable high-molecular polymer can be polycaprolactone (PCL), polylactic acid (PLA, containing PLLA), gather
Any one in glycolic (PGA), PPDO (PPDO), poly (glycolide-lactide) (PLGA), it is also possible to be
One in its copolymer, such as PPDO-PLLA, PLLA-PCL, PLLA-PGA etc..
Described modification can be the modification mode such as blending and modifying, filling-modified, chemical modification, as long as modified material can be real
Existing technical scheme of the present invention.
The beneficial effects of the present invention is: the present invention take biofilm matrix as host material, by with biodegradable high score
The blending and modifying of sub-polymer and copolymer thereof obtains the male genital organ sticking patch composite of a kind of degradable tool bioelastic,
This composite machinery is functional, it is easy to bending, can metabolism, and this composite Materials Design becomes column or the spiral shell of axially open
Rotation shape, is more suitable for operation and uses.Biofilm matrix uses natural biological film, possesses good elasticity and biocompatibility；Use
Macromolecule polymer material be Biodegradable high-molecular, possess excellent specific property slowly of degrading.
Accompanying drawing explanation
In order to make the purpose of the present invention, technical scheme and beneficial effect clearer, the present invention provides drawings described below:
Fig. 1 is the male genital organ sticking patch composite photo of degradable tool bioelastic；
Fig. 2 is composite photo after bending；
Fig. 3 is the gained composite of the present invention scanning electron microscopic picture under 400 times；
Fig. 4 is the gained composite of the present invention scanning electron microscopic picture under 1000 times；
Fig. 5 is the gained composite of the present invention scanning electron microscopic picture under 2000 times；
Fig. 6 is the column composite form picture axially penetrating through an opening prepared by the present invention；
Fig. 7 is helical form composite form picture prepared by the present invention；
Fig. 8 is the dynamic mechanical curve chart of gained composite of the present invention.
Detailed description of the invention
Embodiment 1
Using submucous layer of small intestine as biofilm matrix raw material, technical scheme is described in detail.
The male genital organ sticking patch composite detailed preparation process of degradable tool bioelastic is:
1) biomembrane is produced: mechanical means removes pig small intestine placenta percreta, placenta percreta, muscle layer, obtains submucous layer of small intestine, repeatedly rushes
Wash standby；
2) defat: the trees-Osima jacoti, Osima excavata of acquirement is placed in soak degreasing in the methanol of volume ratio 1:1 and chloroform mixed solution
12h, deionized water rinses 3 times；
3) de-cell: the trees-Osima jacoti, Osima excavata material after above-mentioned defat is placed in the normal saline containing pancreatin and EDTA 4 DEG C
Vibration digestion 48h, ionized water cleans 3 times repeatedly；The weight/mass percentage composition of described pancreatin and EDTA is 0.05%；Described life
Reason saline is the sodium chloride solution of mass fraction 0.9%；
4) soak and pull an oar: the trees-Osima jacoti, Osima excavata after de-cell is cut into 2cm segment, adding the ice of volume fraction 0.5%
4 DEG C of vibration soaked overnight in acetum；Put into after immersion in beater and pull an oar, for avoiding heating to cause in operating process
Protein denaturation, adds the glacial acetic acid solution of appropriate volume fraction 0.5%, can also reduce the viscosity of serosity simultaneously；
5) enzymolysis, saltout: the liquid after making beating is added pepsin enzymolysis (after pepsic consumption takes off cell with defat
Biofilm weight meter, every gram is 4000U by enzyme amount), 4 DEG C freeze room digestion 48h；With gauze by pepsin enzyme after digestion
The submucous layer of small intestine serosity solved filters, and filtrate puts into 4 DEG C of 12h that saltout in 2mol/L NaCl solution, substantially divides seen from after saltouing
Layer；Being put in 4 centrifuge tubes, be placed in high speed centrifuge 4 DEG C, 8000rpm is centrifuged 45min；
6) powder is prepared: taken out by the solid matter after centrifugal, in the glacial acetic acid solution of addition volume fraction 0.5% under the conditions of 4 DEG C
Dissolving, the solution dialysis after dissolving, to remove pepsin and NaCl, specially loads molecular cut off (MWCO) 7000D
Bag filter in, be put in deionized water dialysis, every 4h changes water once, and dialyse 48h altogether；The solution dialysed is placed in cultivation
In ware, shape 12h at-20 DEG C of refrigerator freezings, be then placed in low-temperature freeze-drying machine ,-95 DEG C of negative pressure lyophilizing, keep negative pressure to exist
0.2hPa (hundred handkerchiefs) following 48h；The material of lyophilizing is placed in analysis grinder grind and beat powder and make powder；
7) composite is prepared: with chloroform by Biodegradable high-molecular polymer poly caprolactone (PCL) and step 6) prepare
Powder dissolve, pour in mould, be immersed in the water 2h molding, at-20 DEG C of refrigerator freezing 4h after taking-up, put into low temperature cold lyophilizing
In dry machine ,-95 DEG C of negative pressure lyophilizing, keep negative pressure at 0.2hPa (hundred handkerchiefs) following 48h, finally obtain the biological bullet of degradable tool
The male genital organ sticking patch composite of property.
Embodiment 2
With the pericardium of pig for biofilm matrix raw material, technical scheme is described in detail.
The male genital organ sticking patch composite detailed preparation process of degradable tool bioelastic is:
1) take biomembrane: the heart of pig is separated with pericardium by mechanical means, and by the fatty scraped clean on pericardium, repeatedly rush
Wash the pericardium of clean near-transparent shape is standby；
2) defat: the pericardium of acquirement is placed in soak degreasing 12h in the methanol of volume ratio 1:1 and chloroform mixed solution, go from
Sub-water rinses 3 times；
3) de-cell: the pericardium material after above-mentioned defat is placed in 4 DEG C of vibrations in the normal saline containing pancreatin and EDTA and disappears
Changing 48h, ionized water cleans 3 times repeatedly；The weight/mass percentage composition of described pancreatin and EDTA is 0.05%；Described normal saline
Sodium chloride solution for mass fraction 0.9%；
4) soaking and pull an oar: the pericardium after de-cell is cut into 2*2cm fritter, the glacial acetic acid adding volume fraction 0.5% is molten
4 DEG C of vibration soaked overnight in liquid；Put into after immersion in beater and pull an oar, for avoiding heating to cause protein in operating process
Degeneration, adds the glacial acetic acid solution of appropriate volume fraction 0.5%, can also reduce the viscosity of serosity simultaneously；
5) enzymolysis, saltout: the liquid after making beating is added pepsin enzymolysis (after pepsic consumption takes off cell with defat
Biofilm weight meter, every gram is 4000U by enzyme amount), 4 DEG C freeze room digestion 48h；With gauze by pepsin enzyme after digestion
The pericardium serosity solved filters, and filtrate puts into 4 DEG C of 12h that saltout in 2mol/L NaCl solution, visible substantially layering after saltouing；Will
It is put in 4 centrifuge tubes, is placed in high speed centrifuge 4 DEG C, and 8000rpm is centrifuged 45min；
6) powder is prepared: taken out by the solid matter after centrifugal, in the glacial acetic acid solution of addition volume fraction 0.5% under the conditions of 4 DEG C
Dissolving, the solution dialysis after dissolving, to remove pepsin and NaCl, specially loads molecular cut off (MWCO) 7000D
Bag filter in, be put in deionized water dialysis, every 4h changes water once, and dialyse 48h altogether；The solution dialysed is placed in cultivation
In ware, shape 12h at-20 DEG C of refrigerator freezings, be then placed in low-temperature freeze-drying machine ,-95 DEG C of negative pressure lyophilizing, keep negative pressure to exist
0.2hPa (hundred handkerchiefs) following 48h；The material of lyophilizing is placed in analysis grinder grind and beat powder and make powder；
7) composite is prepared: with TEG by 10wt% Biodegradable high-molecular polymer P GA and 0.6wt%F127
Mixing at 90 DEG C, with step 6 when solution temperature to be mixed drops to 40 DEG C) powder (5wt%) prepared mixes, and pours into
In mould, it is immersed in the water 2h molding, at-20 DEG C of refrigerator freezing 4h after taking-up, puts in low-temperature freeze-drying machine ,-95 DEG C of negative pressure
Lyophilizing, keeps negative pressure to mend at 0.2hPa (hundred handkerchiefs) following 48h, the male genital organ finally obtaining degradable tool bioelastic
Sheet composite.
Described Biodegradable high-molecular polymer can also is that polylactic acid (PLA, containing PLLA), PPDO
(PPDO), any one in poly (glycolide-lactide) (PLGA), it is also possible to be the one in its copolymer, such as PPDO-PLLA,
PLLA-PCL, PLLA-PGA etc..Biodegradable high-molecular polymer is dissolved in solvent and can use fusion method, with F127
As catalyst, be heated to 90-160 DEG C of mixing, treat solution be down to less than 40 DEG C stand-by.
Fig. 1 is male genital organ sticking patch composite photo；Fig. 2 is composite state after bending, and institute of the present invention is described
Obtain composite machinery functional, it is easy to bending；Fig. 3-5 is the embodiment of the present invention 1 gained composite 400,1000,
Scanning electron microscopic picture under 2000 times, it is seen that diameter is in the space of 50-200 μm, and this space is conducive to autogenous cell and tissue
Growing into, meanwhile, this sponge structure also can be as the carrier of some activity modifying materials (such as somatomedin and polypeptide)；Fig. 6-7
The helical form prepared for the present invention and the column composite form picture axially penetrating through an opening, be prepared as this shape and be more suitable for hands
Art uses.Fig. 8 is the dynamic mechanical curve of the composite sample of the embodiment of the present invention 1 gained, and this test is at DMA
Q800 type dynamic thermomechanical analysis apparatus is carried out, and sample makes the standard batten of thin film stretching, measuring samples size: 12.3578
× 6.2800 × 0.7200mm, arranges " multi-frequency, strain " pattern, and heating rate is 3 DEG C of min-1, angular frequency 1Hz.
Test result storage modulu G of this material ' far above loss modulus G ", this illustrates that this composite panels presents elastomeric material
Performance and non-cohesive material feature.
Finally illustrating, preferred embodiment above is only in order to illustrate technical scheme and unrestricted, although by above-mentioned
The present invention is described in detail by preferred embodiment, it is to be understood by those skilled in the art that can in form and
In details, it is made various change, without departing from claims of the present invention limited range.
Claims (10)
1. the male genital organ sticking patch composite of a degradable tool bioelastic, it is characterised in that by biofilm matrix powder
End forms with Biodegradable high-molecular polymer in mass ratio 4～1:1～4, by biofilm matrix powder and biodegradable high score
Sub-polymer is dissolved in solvent after being prepared as solution and pours die for molding into, is then removed from the molds and lyophilization obtains；
Described biofilm matrix powder be biomembrane through defat, de-cell, except antigen after the powder made.
The male genital organ sticking patch composite of a kind of degradable tool bioelastic the most according to claim 1, its feature
Being, described Biodegradable high-molecular polymer is pla-pcl, polylactic acid, polyglycolic acid, PPDO, gathers
Polymer after second lactide or their modifications by copolymerization.
The male genital organ sticking patch composite of a kind of degradable tool bioelastic the most according to claim 1, its feature
Being, described biomembrane is the submucous layer of small intestine of animal, mucous membrane of urinary bladder, pericardium, skin or the animal limb tendons.
The male genital organ sticking patch composite of a kind of degradable tool bioelastic the most according to claim 1, its feature
It is, described includes that the biomembrane after defat is taken off cell uses pepsin enzymolysis after being beaten into liquid except the step of antigen, saltout
Step.
The male genital organ sticking patch composite of a kind of degradable tool bioelastic the most according to claim 1, its feature
Being, described solvent is hexafluoroisopropanol, chloroform, TEG, carbon tetrachloride or ethyl acetate.
The male genital organ sticking patch composite of a kind of degradable tool bioelastic the most according to claim 1, its feature
Being, described mould is composite can be fixed to helical form or axially penetrate through the mould of column of an opening.
7. the preparation of the male genital organ sticking patch composite of a kind of degradable tool bioelastic described in any one of claim 1～6
Method, it is characterised in that comprise the steps:
1) biomembrane is produced；
2) defat: by step 1) obtain biomembrane be placed in soak degreasing 12h in the methanol-chloroform mixed liquor of volume ratio 1:1, go
Ionized water rinses 3 times；
3) de-cell: by step 2) material after defat is placed in the normal saline containing pancreatin and EDTA, and 4 DEG C of vibrations disappear
Changing 48h, ionized water cleans 3 times repeatedly；The weight/mass percentage composition of described pancreatin and EDTA is 0.05%；
4) soak and pull an oar: by step 3) process after material be cut in the glacial acetic acid solution adding volume fraction 0.5% after segment
4 DEG C are soaked 12h, making beating, add the glacial acetic acid solution of volume fraction 0.5% in pulping process, it is to avoid heating causes protein denaturation；
5) enzymolysis, saltout: the liquid after making beating is added enzymolysis 48h under the conditions of pepsin 4 DEG C, filters after enzymolysis and obtain
Obtain filtrate；Filtrate is put into 4 DEG C of 12h that saltout in the NaCl solution of 2mol/L, and under the conditions of 4 DEG C, 8000rpm is centrifuged 45min,
Obtain solids；
6) prepare powder: by step 5) centrifugal after the solids that obtains molten with the glacial acetic acid of volume fraction 0.5% under the conditions of 4 DEG C
Liquid dissolves, dialysis, and powder is made in lyophilizing；
7) composite is prepared: with solvent by Biodegradable high-molecular polymer and step 6) powder that obtains dissolves, pours into
In mould, mould is immersed in the water 2h molding；Material after molding is taken out and is placed in-20 DEG C of freezing 4h ,-95 DEG C of negative pressure lyophilizing,
Negative pressure is kept to obtain the male genital organ sticking patch composite of degradable tool bioelastic after 0.2hPa (hundred handkerchiefs) following 48h.
Preparation method the most according to claim 7, it is characterised in that described step 5) described in enzymolysis time pepsin
Consumption take off in terms of the biofilm weight after cell by defat, every gram is 4000U by enzyme amount.
Preparation method the most according to claim 7, it is characterised in that described step 6) described in dialyse use dialysis
Bag molecular cut off 7000D, the dialysis solution of use is deionized water.
Preparation method the most according to claim 7, it is characterised in that described step 6) described in lyophilizing first at-20 DEG C
Freezing shaping 12h, then-95 DEG C of negative pressure lyophilizing, keep negative pressure 48h below 0.2 hundred handkerchiefs.
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