CN106860918A - A kind of method that skin histology is built based on biological 3D printing - Google Patents
A kind of method that skin histology is built based on biological 3D printing Download PDFInfo
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- CN106860918A CN106860918A CN201710038135.0A CN201710038135A CN106860918A CN 106860918 A CN106860918 A CN 106860918A CN 201710038135 A CN201710038135 A CN 201710038135A CN 106860918 A CN106860918 A CN 106860918A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/60—Materials for use in artificial skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
- A61L27/3612—Cartilage, synovial fluid
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
- A61L27/362—Skin, e.g. dermal papillae
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
- A61L27/3687—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by the use of chemical agents in the treatment, e.g. specific enzymes, detergents, capping agents, crosslinkers, anticalcification agents
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y10/00—Processes of additive manufacturing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y70/00—Materials specially adapted for additive manufacturing
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/40—Preparation and treatment of biological tissue for implantation, e.g. decellularisation, cross-linking
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Abstract
The present invention discloses the method for building skin histology based on biological 3D printing, and step is：Grease removal, skin histology；Processed using acid-enzyme binding-method, take supernatant；Saltoutd to potassium carbonate, sodium carbonate, sodium chloride, potassium chloride, potassium sulfate or sodium sulphate is added in crude extract；The collagen that will be obtained is poured into the Photocopy Room of biometric print machine as the ink raw material of printing skin, and Photocopy Room temperature control is 4 oC；It is 37 by being laminated printing technique in temperature oCThermal station on print arbitrary shape skin；The skin of printing is placed in freeze-drying in container；It is crosslinked 6 hours during lyophilized skin histology is placed in into formaldehyde or glutaraldehyde, and is cleaned with phosphate buffer；Finally it is immersed in lyophilized preservation in ethanol water.The present invention is easy to operate, can effectively facilitate skin repair, can provide beneficial reference for the clinical treatment of the scientific research of artificial skin engineering and skin injury, and the effective ways and technical support of clinicization are provided for diseases such as treatment skin trauma and burns.
Description
Technical field
The invention belongs to biology and organizational engineering technical field, and in particular to one kind builds skin based on biological 3D printing
The method of skin tissue.
Background technology
Skin is to be covered in Whole Body surface to have the organ of important physiological function, is made up of epidermis and skin corium.
When skin occurrence of large-area wound or missing, fatal harm will be caused to human body.Preferably repair, instead of defect
Skin histology should possess following feature：Skin wound can effectively be covered；Possesses certain mechanical strength；Gradually
Promote subcutaneous cell and angiogenic growth, form Autologous epidermis layer and corium Rotating fields and farthest grow to normal skin
Healing quality；It is economical and easily available, easy to use.
With the fast development of tissue engineering technique, organization engineering skin has turned into the focus of defect of skin treatment, early stage
Artificial skin or skin replacement product also have a lot, the artificial skin preparation cost being related in its technology is more high, while
Also increase the difficulty for ensureing that material tight is combined.Therefore, skin tissue engineering field is in the urgent need to developing a kind of new promotion
The method of the sponge material of skin repair, promotes skin repair purpose to be prepared into while can also effectively alleviate can effectively reach
This height and the defect that time-consuming.
Collagen is that vertebrate in-vivo content is most abundant, is distributed widest one group of scleroprotein, is largely present in bone, soft
In bone, tendon and skin, the 25%-33% of human body or other animal body total protein contents is accounted for.Wherein type i collagen accounts for the total glue of organism
The 90% of commercial weight, therefore use of the type i collagen in biomaterial is also the most extensive.The collagen sponge abundance in pigskin source
Economical and easily available, recovery rate is high, and it is similar to human collagen protein structure, is well suited for the reparation and regeneration of human organ.
The skin tissue repair material of this biological 3D printing is conformed into fibrocyte and blood vessel after site of injury gradually long
Enter spongy layer, the matrix components similar to skin are formed after 2-3 weeks.Therefore moving for cell at defective tissue can effectively be induced
Move, breed and break up.Using the artificial skin of collagen sponge structure induction defect skin regeneration in situ, can greatly shorten wound
Mouth healing cycle.Further, it is also possible to strengthen the skin elasticity after wound healing, pliability and reduce scar hyperplasia.
The content of the invention
The purpose of the present invention is to solve the shortcomings of the prior art, a kind of base that can be greatly facilitated skin repair is now provided
In the method that biological 3D printing builds skin histology.
In order to solve the above technical problems, the technical solution adopted by the present invention is：One kind builds skin based on biological 3D printing
The method of tissue, its innovative point is：Comprise the following steps：
（1）Grease removal, separates fish-skin, cartilage, tendon, beef tendon, pig's feet or skin histology；
（2）Processed using acid-enzyme binding-method, add pepsin digestion to extract 3 days, take supernatant；
（3）Saltoutd to potassium carbonate, sodium carbonate, sodium chloride, potassium chloride, potassium sulfate or sodium sulphate is added in crude extract；
（4）The collagen that will be obtained is poured into the Photocopy Room of biometric print machine as the ink raw material of printing skin, prints room temperature
Degree control is 4 oC；
（5）It is 37 by being laminated printing technique in temperature oCThermal station on print the skin of arbitrary shape and size；
（6）The skin that will be printed is placed in freeze-drying in container；
（7）It is crosslinked 6 hours during lyophilized skin histology is placed in into formaldehyde or glutaraldehyde, and is washed with phosphate buffer or pure water
Only；
（8）Finally it is immersed in lyophilized preservation in ethanol water.
Further, the step（1）In careful removal subcutaneous fat, tissue is cut into small pieces, temperature control is in 22-
30oC, with pure water, is put under normal temperature after drying and is positioned over 4 oCIt is standby in refrigerator.
Further, the step（2）Middle that tissue is soaked in acetum, extractive process is in 4 oCCarried out under environment,
The pepsin additional proportion is 10:1.
Further, the step（3）It is middle that filtrate is stirred well to the complete Precipitation of collagen, 10000 revs/min from
The heart 15 minutes, collects precipitation.
Further, the step（4）It is middle that collagen is moved to 4 oCPhotocopy Room is simultaneously mixed, and eliminates bubble.
Further, the step（5）In every layer of print thickness be 500 microns, print ten layers altogether, 5 millimeters of gross thickness,
The humidity of the print platform is 95%.
Further, the step（6）It is middle that printed skin histology is positioned over rapidly -80 oCLower freezing, then by it
It is transferred in freeze drier and freezes.
Further, the step（7）It is middle that lyophilized skin histology is completely soaked in crosslinking agent, friendship is removed after crosslinking
Connection agent, is cleaned with phosphate buffer or pure water.
Further, the step（8）In carefully skin histology is taken out and is soaked in ethanol water, it is suitable to be placed in
Continue freeze-drying in container.
Beneficial effects of the present invention are as follows：The inventive method is reliable, easy to operate, can effectively facilitate skin repair, energy
It is treatment skin trauma enough for the clinical treatment of scientific research and the skin injury of artificial skin engineering provides beneficial reference
And burn etc. disease provide clinicization effective ways and technical support.
Brief description of the drawings
Fig. 1 is dry by skin histology freezing is printed in a kind of method that skin histology is built based on biological 3D printing of the present invention
Form after dry；
Fig. 2 be the present invention it is a kind of based on biological 3D printing build skin histology method in by cck-8 methods detect collagen sponge
Biocompatibility is analyzed；
Fig. 3 be the present invention it is a kind of based on biological 3D printing build skin histology method in by cck-8 methods detect skin histology
Biocompatibility is analyzed；
Fig. 4 be the present invention it is a kind of based on biological 3D printing build skin histology method in by cck-8 methods detection printing skin
The influence of cell growth.
Specific embodiment
Embodiments of the present invention are illustrated by particular specific embodiment below, those skilled in the art can be by this explanation
Content disclosed by book understands other advantages of the invention and effect easily.
A kind of method that skin histology is built based on biological 3D printing, is comprised the following steps：
（1）Grease removal, separates fish-skin, cartilage, tendon, beef tendon, pig's feet or skin histology；Careful removal subcutaneous fat, will organize
It is cut into small pieces, temperature control is in 22-30oC, with pure water, is put under normal temperature after drying and is positioned over 4 oCIt is standby in refrigerator.
（2）Processed using acid-enzyme binding-method, add pepsin digestion to extract 3 days, take supernatant；Tissue is soaked in vinegar
In acid solution, extractive process is in 4 oCCarried out under environment, pepsin additional proportion is 10:1.
（3）Saltoutd to potassium carbonate, sodium carbonate, sodium chloride, potassium chloride, potassium sulfate or sodium sulphate is added in crude extract；Will filter
Liquid is stirred well to the complete Precipitation of collagen, and 10000 revs/min are centrifuged 15 minutes, collect precipitation.
（4）The collagen that will be obtained is poured into the Photocopy Room of biometric print machine as the ink raw material of printing skin, is printed
Room temperature is controlled 4 oC；Collagen is moved to 4 oCPhotocopy Room is simultaneously mixed, and eliminates bubble.
（5）It is 37 by being laminated printing technique in temperature oCThermal station on print the skin of arbitrary shape and size；Every layer
Print thickness is 500 microns, altogether ten layers of printing, 5 millimeters of gross thickness, and the humidity of print platform is 95%.
（6）The skin that will be printed is placed in freeze-drying in container；Printed skin histology is positioned over rapidly -80 oCUnder
Freezing, is then transferred in freeze drier and is freezed.
（7）It is crosslinked 6 hours during lyophilized skin histology is placed in into formaldehyde or glutaraldehyde, and with phosphate buffer or pure water
Clean；Lyophilized skin histology is completely soaked in crosslinking agent, crosslinking agent is removed after crosslinking, with phosphate buffer or pure water
Clean.
（8）Finally it is immersed in lyophilized preservation in ethanol water；It is careful to take out skin histology and to be soaked in ethanol water-soluble
In liquid, it is placed in and continues freeze-drying in suitable vessel.
In order to further appreciate that the effect of the inventive method, the skin histology to being printed has carried out corresponding identification.
（1）Cck-8 cell biologicals compatibility is detected
Skin histology is completely dissolved in the watery hydrochloric acid of pH=3,1 volume dermal solutions and 9 volumes are proportionally respectively configured
The nutrient solution of nutrient solution and 2 volume dermal solutions and 8 volumes, control group similarly uses watery hydrochloric acid and the culture of above-mentioned volume ratio
Liquid replaces.HUVEC cells are inoculated in 96 orifice plates, cell density is 8x104/ mL, 100 μ L are added per hole, treat cell growth
Liquid is changed after to 80%, the dermal solutions that addition is prepared continue to cultivate 24 hours, 48 hours, 72 hours.Suck old nutrient solution and add and match somebody with somebody
The good μ L of cck-8 solution 100 are incubated 2-3 hours, liquid is transferred in new 96 orifice plate and detects absorbance under 450nm wavelength.
（2）3D culture of the cell on the skin histology of printing
The skin histology of printing is cut into 48 hole sizes and is placed in orifice plate bottom, 300 μ L BMSCs cell suspensions are uniformly added
In on sponge, wherein cell density is 1x105/ mL, liquid was changed every 3 days.It is placed in culture 3 days in incubator, after 14 days, inhales respectively
Remove old nutrient solution and add the μ L of cck-8 solution 100 that prepare to be incubated 2-3 hours, liquid is transferred to 450nm ripples in new 96 orifice plate
Lower detection absorbance long.
As Fig. 1 by the present invention it is a kind of based on biological 3D printing build skin histology method in printed skin histology freeze
Dried form.
As Fig. 2 be the present invention it is a kind of based on biological 3D printing build skin histology method in by cck-8 methods detect glue
Olynthus biocompatibility is analyzed.1 part of skin histology is well mixed with 9 parts of nutrient solutions, respectively detect 24 hours, 48 hours, 72
The absorbance of cell after hour, as a result finds that the different collagen sponge of crosslinking degree is respectively provided with preferable biocompatibility.
Control separate cell culture groups, non:Skin group, heat crosslinking are not crosslinked:Heat cross-linking sponge group,
glutaraldehyde crosslinking:Chemical crosslinking group
As Fig. 3 be the present invention it is a kind of based on biological 3D printing build skin histology method in by cck-8 methods detect skin group
Knit biocompatibility analysis.2 parts of skin histologies are well mixed with 8 parts of nutrient solutions, detect 24 hours, 48 hours, 72 hours respectively
The absorbance of cell, as a result finds that the different collagen sponge of crosslinking degree is respectively provided with preferable biocompatibility, Er Qiejiao afterwards
The propagation of cell can be obviously promoted within 24 hours after connection in culture（p < 0.01）, wherein be chemically crosslinked culture 48 hours it is brighter
It is aobvious（p < 0.001）.
Fig. 4 be the present invention it is a kind of based on biological 3D printing build skin histology method in by cck-8 methods detect print
The influence of skin cell growth.The skin histology that will have been pruned is put into 48 orifice plates, detect respectively the 3rd day, it is thin after the 14th day
The absorbance of born of the same parents.Result finds that heat cross-linking, chemical crosslinking sponge OD values compared with sponge is not crosslinked substantially increase（p <
0.05）.Heat cross-linking or chemical crosslinking sponge can be obviously promoted cell and grow into sponge when showing 14 days, and cell growth has obvious
Facilitation.
The inventive method is reliable, easy to operate, can effectively facilitate skin repair, can be the science of artificial skin engineering
The clinical treatment of research and skin injury provides beneficial reference, for the diseases such as treatment skin trauma and burn provide clinicization
Effective ways and technical support.
Above-described embodiment is presently preferred embodiments of the present invention, is not the limitation to technical solution of the present invention, as long as
Without the technical scheme that creative work can be realized on the basis of above-described embodiment, it is regarded as falling into patent of the present invention
Rights protection scope in.
Claims (9)
1. it is a kind of based on biological 3D printing build skin histology method, it is characterised in that：Comprise the following steps：
（1）Grease removal, separates fish-skin, cartilage, tendon, beef tendon, pig's feet or skin histology；
（2）Processed using acid-enzyme binding-method, add pepsin digestion to extract 3 days, take supernatant；
（3）Saltoutd to potassium carbonate, sodium carbonate, sodium chloride, potassium chloride, potassium sulfate or sodium sulphate is added in crude extract；
（4）The collagen that will be obtained is poured into the Photocopy Room of biometric print machine as the ink raw material of printing skin, prints room temperature
Degree control is 4 oC；
（5）It is 37 by being laminated printing technique in temperature oCThermal station on print the skin of arbitrary shape and size；
（6）The skin that will be printed is placed in freeze-drying in container；
（7）It is crosslinked 6 hours during lyophilized skin histology is placed in into formaldehyde or glutaraldehyde, and is washed with phosphate buffer or pure water
Only；
（8）Finally it is immersed in lyophilized preservation in ethanol water.
2. it is according to claim 1 it is a kind of based on biological 3D printing build skin histology method, it is characterised in that：It is described
Step（1）In careful removal subcutaneous fat, tissue is cut into small pieces, temperature control is in 22-30oC, with pure water, is put in often
4 are positioned over after being dried under temperature oCIt is standby in refrigerator.
3. it is according to claim 1 it is a kind of based on biological 3D printing build skin histology method, it is characterised in that：It is described
Step（2）Middle that tissue is soaked in acetum, extractive process is in 4 oCCarried out under environment, the pepsin additional proportion
It is 10:1.
4. it is according to claim 1 it is a kind of based on biological 3D printing build skin histology method, it is characterised in that：It is described
Step（3）Middle that filtrate is stirred well into the complete Precipitation of collagen, 10000 revs/min are centrifuged 15 minutes, collect precipitation.
5. it is according to claim 1 it is a kind of based on biological 3D printing build skin histology method, it is characterised in that：It is described
Step（4）It is middle that collagen is moved to 4 oCPhotocopy Room is simultaneously mixed, and eliminates bubble.
6. it is according to claim 1 it is a kind of based on biological 3D printing build skin histology method, it is characterised in that：It is described
Step（5）In every layer of print thickness be 500 microns, print ten layers altogether, 5 millimeters of gross thickness, the humidity of the print platform is
95%。
7. it is according to claim 1 it is a kind of based on biological 3D printing build skin histology method, it is characterised in that：It is described
Step（6）It is middle that printed skin histology is positioned over rapidly -80 oCLower freezing, is then transferred in freeze drier and is freezed.
8. it is according to claim 1 it is a kind of based on biological 3D printing build skin histology method, it is characterised in that：It is described
Step（7）It is middle that lyophilized skin histology is completely soaked in crosslinking agent, crosslinking agent is removed after crosslinking, with phosphate buffer or
Pure water is cleaned.
9. it is according to claim 1 it is a kind of based on biological 3D printing build skin histology method, it is characterised in that：It is described
Step（8）In carefully skin histology is taken out and is soaked in ethanol water, be placed in and continue freeze-drying in suitable vessel.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710038135.0A CN106860918A (en) | 2017-01-19 | 2017-01-19 | A kind of method that skin histology is built based on biological 3D printing |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710038135.0A CN106860918A (en) | 2017-01-19 | 2017-01-19 | A kind of method that skin histology is built based on biological 3D printing |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110960730A (en) * | 2019-12-23 | 2020-04-07 | 吉林大学 | 3D printed bionic rejection-resistant artificial skin and preparation method thereof |
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| CN104068945A (en) * | 2014-06-27 | 2014-10-01 | 深圳齐康医疗器械有限公司 | Artificial skin and preparation method thereof |
| CN105885436A (en) * | 2016-04-26 | 2016-08-24 | 中山大学附属第医院 | Biological ink material for 3D printing and preparation method and application thereof |
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2017
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| CN103432627A (en) * | 2013-08-26 | 2013-12-11 | 北京瑞健高科生物科技有限公司 | Method for preparing animal acellular tissue matrix material and tissue matrix material prepared by same |
| CN104068945A (en) * | 2014-06-27 | 2014-10-01 | 深圳齐康医疗器械有限公司 | Artificial skin and preparation method thereof |
| CN105885436A (en) * | 2016-04-26 | 2016-08-24 | 中山大学附属第医院 | Biological ink material for 3D printing and preparation method and application thereof |
| CN105920670A (en) * | 2016-05-19 | 2016-09-07 | 李世荣 | Degradable male genital organ patch composite material with biological elasticity and preparation method thereof |
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| FALGUNI PATI 等: "Printing three-dimensional tissue analogues with decellularized extracellular matrix bioink", 《NATURE COMMUNICATIONS》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110960730A (en) * | 2019-12-23 | 2020-04-07 | 吉林大学 | 3D printed bionic rejection-resistant artificial skin and preparation method thereof |
| CN110960730B (en) * | 2019-12-23 | 2021-10-26 | 吉林大学 | 3D printed bionic rejection-resistant artificial skin and preparation method thereof |
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