CN103054628A - Method for fast constructing and repairing skin defects by tissue engineering skins - Google Patents
Method for fast constructing and repairing skin defects by tissue engineering skins Download PDFInfo
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- CN103054628A CN103054628A CN 201310035216 CN201310035216A CN103054628A CN 103054628 A CN103054628 A CN 103054628A CN 201310035216 CN201310035216 CN 201310035216 CN 201310035216 A CN201310035216 A CN 201310035216A CN 103054628 A CN103054628 A CN 103054628A
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- skin
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- skin graft
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/36—Skin; Hair; Nails; Sebaceous glands; Cerumen; Epidermis; Epithelial cells; Keratinocytes; Langerhans cells; Ectodermal cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/60—Materials for use in artificial skin
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- Animal Behavior & Ethology (AREA)
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Abstract
The invention discloses a method for fast constructing and repairing skin defects by tissue engineering skins and belongs to the field of medical methods. The method can be used for solving the defects of low survival rate of skin grafts and long period of the traditional medical method, wherein the method is formed by a razor-thin graft and a cell suspension in combination with a composite skin grafting method. The method for fast constructing and repairing the skin defects by the tissue engineering skins has good effects of promoting wound healing, enhancing the survival rate of the razor-thin graft, and improving the color and texture of skins, and can effectively treat skin defects resulted from various reasons such as a trauma and a surgery.
Description
Technical field
The present invention relates to the therapy field, particularly a kind of quick organization engineering skin makes up the method for used for reparing skin defect.
Background technology
The large skin defect that a variety of causes causes is the subject matter that burns unit, shaping reconstructive surgery face such as burn, wound.In order effectively to solve the problem of skin source and the rear effect of reparation, new technology and method constantly occurs.Grafts, skin flap transplantation, stamp grafts, microparticle skin transplanting, netted skin grafting dermepenthesis, Composite skin etc.The research of organization engineering skin is also constantly being goed deep into, present organization engineering skin comprises from body or allosome epidermis sheet dermal substitute and contains epidermis and 3 kinds of double-deck Graftskins of corium, and best substitute still is Autologous epidermis sheet dermal substitute, because remaining great majority only produce effects in the patient of chronic ulcer and burn, simultaneously they also all in various degree existence cut difficulty, be easy to infection, immunologic rejection and irritated etc.Cultivate the epidermis sheet owing to lack the support of corium composition, extremely sensitive to mechanical damage, transplant rear Wound Contraction and the scar hyperplasia of easily forming, and owing to lack corium, do not realize real skin reconstruction.Yet Autologous epidermis sheet dermal substitute is owing to lack epidermis, and without living cells, easy infection is transplanted rear easily degraded, shunk, and easily forms cicatrix at the wound surface edge.
Although new method is all improved and the treatment level that has advanced wound repair to a certain extent, also all exist weak point separately, it is low to overcome the survival of grafted skin rate, the shortcoming that production life cycle is long.
Summary of the invention
A kind of quick organization engineering skin makes up the method for used for reparing skin defect, formed in conjunction with the method for Composite skin by razor graft, cell county liquid, wherein: the application dermatome cuts from the thick skin graft of body sword for subsequent use, and the skin graft area should be greater than the wound surface area about about 10% of need reparation; To take off the wound surface that cell Allodermis Matrix substrate is covered in required covering, its surface get before covering again from the thick skin graft of body sword, prune out unnecessary skin graft; Again with pallium cell injection or be sprayed onto in the Composite Skin skin-grafting area that carries out previously, through the skin graft edge with pallium cell injection to skin graft and take off between the cell Allodermis Matrix substrate; Carry out post surgery treatment by the method for Composite skin again.
As a further improvement on the present invention: described cell suspension is made by skin graft, places 0.2% trypsin solution that is heated to 37 degree to soak digestion 15 ~ 20 minutes skin graft, and epidermis is separated with corium; Digestion time may factor be different decides because of skin graft thickness, room temperature etc.; From trypsin solution, take out skin graft with aseptic tweezer, use two tweezers, attempt peeling off epidermis and corium; Be difficult for realizing if separate, skin graft is placed the trypsin solution that has heated again, hatched again 5 ~ 10 minutes; Postdigestive skin graft is separated into corium fully and epidermis is two-layer with two tweezers, can appear like this corium-epidermis joint portion, with the fixing skin graft layer of tweezers, from corium and epidermis joint portion surface scraping cell, the flushing of application compound recipe ringer's solution is collected the basal layer cell that scrapes and is made cell suspension with aseptic dissecting knife.
As a further improvement on the present invention: described digestion time take 15 minutes as minimum, total digestion time is no more than 60 minutes.
Compared with prior art, the invention has the beneficial effects as follows: wound healing, raising the livabitity of skin grafts, the rear skin colour quality of improving.
The specific embodiment
The inventor uses acellular corium when damaged and has accumulated rich experience with Composite skin from body thin skin sheet repairing body surface, has obtained preferably clinical effectiveness.But also existing simultaneously survival rate fails to reach desirable requirement, survives the problem that rear skin graft quality, color and luster and skin function and normal skin still have gap.In the recent period, the inventor is applied to a kind of method of quick acquisition skin base cell suspension clinical, this cell suspension at wound healing, improve the livabitity of skin grafts, improve more after the aspects such as skin colour quality good effect is arranged.Contain basal cell, Langerhans cell, melanocyte and fibroblast and epidermal stem cells through in preliminary this kind of research cell suspension, abundant competent cell group is its reason that produces clinical effectiveness.Epidermal stem cells is one of focus that is applied to as seed cell at present skin tissue engineering research, desirable organization engineering skin material and take off that the cell Allodermis Matrix also is proved to be as support, with the application that combines of these two kinds of technology, obtain a kind of method that fast, effectively constructs complete function skin.
A kind of quick organization engineering skin makes up the method for used for reparing skin defect, formed in conjunction with the method for Composite skin by razor graft, cell county liquid, wherein: the application dermatome cuts from the thick skin graft of body sword for subsequent use, and the skin graft area should be greater than the wound surface area about about 10% of need reparation; To take off the wound surface that cell Allodermis Matrix substrate is covered in required covering, its surface get before covering again from the thick skin graft of body sword, prune out unnecessary skin graft; Because comprising epidermis, desirable artificial skin is connected connection between the two with the corium double-layer structure closely; Have pliability and certain mechanical strength, be transplanted to and namely well attach with wound surface very soon behind the wound surface; Do not carry virus safely; It is ripe that epidermis and corium composition can be finished self propagation, differentiation and function as early as possible, forms closer to physiological nonvolatil Graftskin etc.; So, again with pallium cell injection or be sprayed onto in the Composite Skin skin-grafting area that carries out previously, through the skin graft edge with pallium cell injection to skin graft and take off between the cell Allodermis Matrix substrate; Carry out post surgery treatment by the method for Composite skin again.
Described cell suspension is made by skin graft, places 0.2% trypsin solution that is heated to 37 degree to soak digestion 15 ~ 20 minutes skin graft, and epidermis is separated with corium; Digestion time may factor be different decides because of skin graft thickness, room temperature etc.; From trypsin solution, take out skin graft with aseptic tweezer, use two tweezers, attempt peeling off epidermis and corium; Be difficult for realizing if separate, skin graft is placed the trypsin solution that has heated again, hatched again 5 ~ 10 minutes; Postdigestive skin graft is separated into corium fully and epidermis is two-layer with two tweezers, can appear like this corium-epidermis joint portion, with the fixing skin graft layer of tweezers, from corium and epidermis joint portion surface scraping cell, the flushing of application compound recipe ringer's solution is collected the basal layer cell that scrapes and is made cell suspension with aseptic dissecting knife; Total amount is not excessive during cell suspension processed as stated above, when cell quantity one regularly the suspension total amount more many cells concentration is lower.
Described digestion time take 15 minutes as minimum, skin graft is thicker, when room temperature is low the time of digestion longer, but total digestion time is no more than 60 minutes.
Case 1:
Wu, man, 5 years old.Be admitted to hospital and seek medical advice because examining as " the huge melanocytic naevi of congenital right upper extremity ".Human health screening sees that large skin of infant right upper extremity is black, hypertrophy slightly, and most of dark skin place has and increases thick hair growth.Routine examination has no obvious operation taboo before improving art after being admitted to hospital.Fiber crops descending " the quick organization engineering skin skin grafting of the huge black wool nevus of right upper extremity excision+thigh bark fetching right upper extremity " in trachea.Complete resection right upper extremity melanotic nevus (postoperative pathological is diagnosed as melanocytic naevi) in the art.It is for subsequent use that motor dermatome cuts the thick razor graft of 0.25mm in infant bilateral thigh place.About 6cm2 in institute's bark fetching sheet is used trypsin in 37 degree digestion 15 minutes.The skin graft epidermal area of digestion is separated cell that rear collection is positioned at basal layer, and to be prepared into the about 4ml of cell suspension for subsequent use with skin corium.1ml cell suspension is sprayed on behind the bilateral thigh skin donor site wound surface covers pressure dressing behind the yarn intellectual circle oil yarn.Right upper extremity melanotic nevus cut-away area lattice are sprayed on field of operation with the stromal cell suspension after covering acellular dermal matrix in implant therapy, cover from the body razor graft again, and the skin-grafting area pressure dressing is fixed.The treatments such as the conventional antiinflammatory fluid infusion of postoperative.The dressing outside postoperative was removed in 7 days of infant right upper extremity skin-grafting area sees that most skin-grafting areas survive well.Skin-grafting area skin colour fine texture is seen in postoperative further consultation in three months, and near normal skin, elasticity is good, and function of joint is normal.
In sum, after those of ordinary skill in the art reads file of the present invention, according to this
Bright technical scheme and technical conceive need not creative mental work and make other various corresponding conversion scheme, all belong to the scope that the present invention protects.
Claims (3)
1. a quick organization engineering skin makes up the method for used for reparing skin defect, formed in conjunction with the method for Composite skin by razor graft, cell county liquid, it is characterized in that: the application dermatome cuts from the thick skin graft of body sword for subsequent use, and the skin graft area should be greater than the wound surface area about about 10% of need reparation; To take off the wound surface that cell Allodermis Matrix substrate is covered in required covering, its surface get before covering again from the thick skin graft of body sword, prune out unnecessary skin graft; Again with pallium cell injection or be sprayed onto in the Composite Skin skin-grafting area that carries out previously, through the skin graft edge with pallium cell injection to skin graft and take off between the cell Allodermis Matrix substrate; Carry out post surgery treatment by the method for Composite skin again.
2. make up the method for used for reparing skin defect according to a kind of quick organization engineering skin claimed in claim 1, it is characterized in that: described cell suspension is made by skin graft, place 0.2% trypsin solution that is heated to 37 degree to soak digestion 15 ~ 20 minutes skin graft, epidermis is separated with corium; Digestion time may factor be different decides because of skin graft thickness, room temperature etc.; From trypsin solution, take out skin graft with aseptic tweezer, use two tweezers, attempt peeling off epidermis and corium; Be difficult for realizing if separate, skin graft is placed the trypsin solution that has heated again, hatched again 5 ~ 10 minutes; Postdigestive skin graft is separated into corium fully and epidermis is two-layer with two tweezers, can appear like this corium-epidermis joint portion, with the fixing skin graft layer of tweezers, from corium and epidermis joint portion surface scraping cell, the flushing of application compound recipe ringer's solution is collected the basal layer cell that scrapes and is made cell suspension with aseptic dissecting knife.
3. the method that makes up used for reparing skin defect according to a kind of quick organization engineering skin claimed in claim 2 is characterized in that: the digestion time of described cell suspension take 15 minutes as minimum, total digestion time is no more than 60 minutes.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201310035216 CN103054628A (en) | 2013-01-30 | 2013-01-30 | Method for fast constructing and repairing skin defects by tissue engineering skins |
| PCT/CN2013/088833 WO2014117597A2 (en) | 2013-01-30 | 2013-12-09 | Rapid tissue engineering method for skin construction and repair of skin damage |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201310035216 CN103054628A (en) | 2013-01-30 | 2013-01-30 | Method for fast constructing and repairing skin defects by tissue engineering skins |
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| Publication Number | Publication Date |
|---|---|
| CN103054628A true CN103054628A (en) | 2013-04-24 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201310035216 Pending CN103054628A (en) | 2013-01-30 | 2013-01-30 | Method for fast constructing and repairing skin defects by tissue engineering skins |
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| CN (1) | CN103054628A (en) |
| WO (1) | WO2014117597A2 (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103990179A (en) * | 2014-05-24 | 2014-08-20 | 河北爱能生物科技有限公司 | Method for preparing xenogeneic acellular matrix and product thereof |
| CN103989531A (en) * | 2014-06-04 | 2014-08-20 | 朱家源 | Instrument bag for fast building tissue engineering skin |
| CN104013999A (en) * | 2014-06-11 | 2014-09-03 | 朱家源 | Tissue engineering skin and preparation method thereof |
| CN112391334A (en) * | 2020-11-17 | 2021-02-23 | 深圳清华大学研究院 | Method for preparing epidermal cell suspension |
-
2013
- 2013-01-30 CN CN 201310035216 patent/CN103054628A/en active Pending
- 2013-12-09 WO PCT/CN2013/088833 patent/WO2014117597A2/en active Application Filing
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103990179A (en) * | 2014-05-24 | 2014-08-20 | 河北爱能生物科技有限公司 | Method for preparing xenogeneic acellular matrix and product thereof |
| CN103989531A (en) * | 2014-06-04 | 2014-08-20 | 朱家源 | Instrument bag for fast building tissue engineering skin |
| CN103989531B (en) * | 2014-06-04 | 2016-05-11 | 朱家源 | The instrument bag of rapid build organization engineering skin |
| CN104013999A (en) * | 2014-06-11 | 2014-09-03 | 朱家源 | Tissue engineering skin and preparation method thereof |
| CN104013999B (en) * | 2014-06-11 | 2016-05-11 | 朱家源 | 3D prints organization engineering skin of rapid build and preparation method thereof |
| CN112391334A (en) * | 2020-11-17 | 2021-02-23 | 深圳清华大学研究院 | Method for preparing epidermal cell suspension |
| CN112391334B (en) * | 2020-11-17 | 2023-03-21 | 深圳清华大学研究院 | Method for preparing epidermal cell suspension |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2014117597A2 (en) | 2014-08-07 |
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Application publication date: 20130424 |