The instrument bag of rapid build organization engineering skin
Technical field
The present invention relates to medical instruments technical field, particularly a kind of rapid build organization engineering skinInstrument bag.
Background technology
The surface of a wound is that body is lost because inherent or external factor cause skin complete, and it is repaired alwaysSince be the subject matter in clinical, can be divided into acute wound and chronic wound by the time clinically.Acute wound comprises the major diseases such as burn, wound or scar, skin-color disposition disease, good perniciousThe defect of skin forming after the operation such as tumour excision; And chronic wound comprises traumatic ulcer, diabetesUlcer, vascular ulcer, pressure ulcer, radiation ulcer, neoplastic ulcer, cicatricial are burstUlcer etc. In recent years, the incidence of disease of chronic wound is ascendant trend year by year, and its course of disease is long, difficult healing,Skin graft survival rate is low, and after skin-grafting, skin function is lost, and causes quality of life to decline. It is reported, full generationBoundary treaty has 1% people to be subjected to the puzzlement of refractory wounds, and the medical expense of 5% left and right is used to the surface of a woundRepair. Show according to wound healing association of U.S. data, exceed the treatment through half a year of 60% chronic woundAfter still cannot heal completely, annual payment for medical care is high. Europe is annual drops into taking of chronic wound treatmentWith accounting for 2% of hygiene and health expenditure, meanwhile, the doctor who repairs because of scar proliferation after wound repairTreatment expense is also up to 12,000,000,000 dollars, and these expenses are still increasing every year.
Operation reparation is current primary treatment method. But, no matter be traditional grafts, skinLobe transplanting, stamp skin grafting dermepenthesis, microparticle skin transplanting, netted skin grafting dermepenthesis, Composite skin etc., or newEmerging organization engineering skin (refers to dermal fibroblast and extracellular matrix substitute is mixedArtificial skin, or use merely the extracellular matrix of porous to substitute the corium implantation surface of a wound, on upper surface transplantChrotoplast covers or treats that self epithelium covers the skin that replaces disease damage), all fail at present effectively to solve woundThe difficult problem that face is repaired. Especially a following difficult problem: scar proliferation, sweat after major disease fire victim's rehabilitationAnadenia, skin lose original function, and (the lighter greatly consumes doctor to chronic wound patient protracted course of diseaseTreat resource, the serious harm life such as severe one accompanying infection, septicopyemia).
The apparatus that existing prosthesis is prepared due to needs is many, needs the time of preparation long,Under laboratory condition, carry out passage cultivation, the time needing is long, tends to affect handPostoperative effect.
Summary of the invention
The object of this invention is to provide a kind of instrument bag of rapid build organization engineering skin, can solveAt least one in above-mentioned prior art problem.
According to an aspect of the present invention, provide a kind of instrument bag of rapid build organization engineering skin,Comprise inclusion and be arranged on the surgical supplies in inclusion, surgical supplies comprise digestive ferment solution freeze-dried powder,De-cell Allodermis Matrix matrix, No. 23 knife blades, anodontia tweezers, syringe, syringe needle,The first sodium lactate Ringer ' solution (also claiming equilibrium liquid), the second sodium lactate Ringer ' solution, filter screen, sterilized waterAnd microheater.
In some embodiments, digestive ferment solution freeze-dried powder can be trypsin solution freeze-dried powder.
In some embodiments, anodontia tweezers can be the anodontia tweezers of ophthalmic.
In some embodiments, syringe can comprise two 5mL syringes and two 10mL notesEmitter.
In some embodiments, syringe needle and syringe match, and wherein, inject with 5mLDevice coupling can be tack syringe needle, what mate with 10mL syringe can be Tip-headed needle head.
In some embodiments, the body of the first sodium lactate Ringer ' solution and the second sodium lactate Ringer ' solutionThe long-pending 5mL that is.
In some embodiments, the aperture of filter screen can be 100 μ m.
In some embodiments, sterilized water can be aseptic double-distilled water, altogether 10mL.
In some embodiments, microheater can also be protected heating liquid to 37 in vial DEG CHold constant temperature.
In some embodiments, in inclusion, can be provided with 13 grooves, place successively 10mL'sThe syringe needle of syringe, 10mL, be loaded with sterilized water bottle, be loaded with enzyme solutions freeze-dried powderBottle, microheater, be loaded with sodium lactate Ringer ' solution bottle, be loaded with sodium lactate ringer'sThe syringe of the bottle of family name's liquid, anodontia tweezers, No. 23 knife blades, 5mL, 5mL syringe needle,Filter screen and de-cell Allodermis Matrix matrix. 12 above-mentioned grooves can also be according to the difference of surgical conditionBe divided into two lines or three row with doctor's use habit, adopt different modes of emplacements.
Prepare time of the present invention, every utensil all needs to carry out aseptic sterilization, then encapsulates. Device of the present inventionTool bag has following advantage:
1, quick. In operating room, can complete, approximately 30 minutes consuming time altogether, organize work with the pastJourney skin need carry out passage cultivation etc. under laboratory condition to be compared, and has quick, disposable completingAdvantage.
2, instrument bag of the present invention has seed cell, also has dermal matrix, uses separately of the present inventionInstrument bag can build complete organization engineering skin structure, does not need to increase other apparatus again.
3, instrument bag seed cell of the present invention is basal layer cell, has class multipotential stem cell activity,Have multipotency differentiation function, the organization engineering skin being built into can recover skin function.
4, the not only healing rate quickening of organization engineering skin that uses instrument bag of the present invention to build, and,Institute's skin grafts can recover skin proper function (perspire and pain are touched the function of sense of heat aspect), has skinThe generation of appendicle.
Use time of the present invention, (desirable artificial skin comprises epidermis first to take from body skin graft or artificial skin graftWith corium double-layer structure and be connected between the two closely, it has pliability and certain mechanical strength,After being transplanted to the surface of a wound, well attach with the surface of a wound very soon, there is safety, do not take viruliferous advantage, tableSkin and corium composition can complete self propagation, differentiation and function maturation as early as possible, form closer to physiologyPermanent Graftskin etc.) for subsequent use, skin graft area should be greater than needs the surface of a wound area approximately 10% repairedLeft and right. Autologous skin graft or artificial skin graft are covered on the surface of a wound that needs to repair, prune unnecessary skin graft.
First insert in aseptic double-distilled water bottle with the 10mL syringe in instrument bag, extract aseptic double-distilled water10mL, then aseptic double-distilled water is injected to the bottle that enzyme solutions freeze-dried powder is housed, fully lyase solution freezesDry powder forms enzyme solutions. Approximately, after 2~3min, the skin graft of having pruned is put in enzyme solutions bottle, with micro-Type heater heats about 15min to enzyme solutions bottle. Take out skin graft and put into the first sodium lactate Ringer ' solutionIn, in and digestive ferment. Tear gently the epidermis of skin graft with the anodontia tweezers in instrument bag off, use instrument bagIn No. 23 knife blades on skin graft basalis under basal layer cell shaving. About 20-30 minuteAfter (being no more than at most one hour), take out skin graft, rinse and collect with the second sodium lactate Ringer ' solutionThe basal layer cell scraping, obtains cell suspension. Extract cell with the 5mL syringe in instrument bag outstandingAfter liquid, spray drops on filter screen, filters scurf, and cell suspension is for subsequent use.
In the time of skin-grafting, first on the surface of a wound after debridement, transplant de-cell Allodermis Matrix matrix, then spraying filteringAfter cell suspension, transplant again above-mentioned got autologous skin graft or artificial in de-cell Allodermis Matrix matrixSkin graft, can rapid build organization engineering skin.
Brief description of the drawings
Fig. 1 is the structure of the instrument bag of the rapid build organization engineering skin of one embodiment of the present inventionSchematic diagram.
Detailed description of the invention
Below in conjunction with accompanying drawing, the present invention is further detailed explanation.
Fig. 1 has schematically shown the rapid build organization engineering skin of one embodiment of the present inventionThe structure chart of instrument bag.
As shown in Figure 1, a kind of instrument bag of rapid build organization engineering skin of embodiment, comprisesInclusion and be arranged on the surgical supplies in inclusion.
In inclusion 1, be provided with 13 grooves, place successively the syringe 231 of 10mL, note with 10mLTip syringe needle 241, the bottle that is loaded with sterilized water 200 that emitter 231 is mated, be loaded withBottle, the microheater 29 of 2.5g enzyme solutions freeze-dried powder 25, be loaded with the first Ru SuannalingeshiBottle, the bottle that is loaded with the second sodium lactate Ringer ' solution 272, the nothing of liquid 271 (also claiming equilibrium liquid)The syringe 232 of 22, No. 23 knife blades 21 of tooth tweezers, 5mL, with 232 of 5mL syringesTack syringe needle 242, filter screen 28 and the de-cell Allodermis Matrix matrix 26 of joining. In inclusion 1, go backBe provided with a vacant groove.
Wherein, digestive ferment solution freeze-dried powder 25 is trypsin solution freeze-dried powder; Anodontia tweezers 22 areOphthalmic is anodontia tweezers 22; The first sodium lactate Ringer ' solution 271 and the second sodium lactate Ringer ' solution 272Volume be 5mL; The aperture of filter screen 28 is 100 μ m; Sterilized water 200 is aseptic double-distilled water, bodyAmass as 10mL; Microheater 29 can and keep constant temperature by heating liquid to 37 in vial DEG C.
Prepare time of the present invention, every utensil all needs to carry out aseptic sterilization, then encapsulates. Device of the present inventionTool bag has following advantage:
1, quick. In operating room, can complete, approximately 30 minutes consuming time altogether, organize work with the pastJourney skin need carry out passage cultivation etc. under laboratory condition to be compared, and has quick, disposable completingAdvantage.
2, instrument bag of the present invention has seed cell, also has dermal matrix, uses separately of the present inventionInstrument bag can build complete organization engineering skin structure, does not need to increase other apparatus again.
3, instrument bag seed cell of the present invention is basal layer cell, has class multipotential stem cell activity,Have multipotency differentiation function, the organization engineering skin being built into can recover skin function.
4, the not only healing rate quickening of organization engineering skin that uses instrument bag of the present invention to build, and,Institute's skin grafts can recover skin proper function (perspire and pain are touched the function of sense of heat aspect), has skinThe generation of appendicle.
Use time of the present invention, (desirable artificial skin comprises epidermis first to take from body skin graft or artificial skin graftWith corium double-layer structure and be connected between the two closely, it has pliability and certain mechanical strength,After being transplanted to the surface of a wound, well attach with the surface of a wound very soon, there is safety, do not take viruliferous advantage, tableSkin and corium composition can complete self propagation, differentiation and function maturation as early as possible, form closer to physiologyPermanent Graftskin etc.) for subsequent use, skin graft area should be greater than needs the surface of a wound area approximately 10% repairedLeft and right. Autologous skin graft or artificial skin graft are covered on the surface of a wound that needs to repair, prune unnecessary skin graft.
First the tip syringe needle in instrument bag 241 and 10mL syringe 231 are screwed, then willIt inserts in aseptic double-distilled water bottle, extracts aseptic double-distilled water 10mL. Aseptic double-distilled water 200 is injected to dressHave in the bottle of enzyme solutions freeze-dried powder 25, fully lyase solution freeze-dried powder 25 forms enzyme solutions. Approximately 2~After 3min, the skin graft of having pruned is put in enzyme solutions bottle, enzyme solutions bottle is entered with microheater 29The about 15min of row heating. Take out skin graft put into the first sodium lactate Ringer ' solution 271, in and digestive ferment.Tear gently the epidermis of skin graft off with the anodontia tweezers 22 in instrument bag, by 23 trumpeter's arts in instrument bagBlade 21 on skin graft basalis under basal layer cell shaving. About 20-30 minute is not (super at mostAfter an hour), take out skin graft, rinse and collect with second sodium lactate Ringer ' solution 272 and scrapeBasal layer cell, obtains cell suspension. Tack syringe needle 242 and 5mL syringe 232 are revolvedTightly, then spray and drop on filter screen 28 with its extraction cell suspension, filter scurf.
In the time of skin-grafting, first on the surface of a wound after debridement, transplant de-cell Allodermis Matrix matrix 26, then sprayedCell suspension after filter, transplants above-mentioned got autologous skin graft or people again in de-cell Allodermis Matrix matrixWork skin graft, can rapid build organization engineering skin. Above-described is only enforcements more of the present inventionMode. For the person of ordinary skill of the art, in the prerequisite that does not depart from the invention designUnder, can also make some distortion and improvement, these all belong to protection scope of the present invention.