DE69411015T2 - Waessrige mehrphasige isolierung eines polypeptids - Google Patents

Waessrige mehrphasige isolierung eines polypeptids

Info

Publication number
DE69411015T2
DE69411015T2 DE69411015T DE69411015T DE69411015T2 DE 69411015 T2 DE69411015 T2 DE 69411015T2 DE 69411015 T DE69411015 T DE 69411015T DE 69411015 T DE69411015 T DE 69411015T DE 69411015 T2 DE69411015 T2 DE 69411015T2
Authority
DE
Germany
Prior art keywords
polypeptide
aqueous
phase insulation
enriched
phases
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69411015T
Other languages
English (en)
Other versions
DE69411015D1 (de
Inventor
Stuart Builder
Roger Hart
Philip Lester
John Ogez
David Reifsnyder
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genentech Inc
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Application granted granted Critical
Publication of DE69411015D1 publication Critical patent/DE69411015D1/de
Publication of DE69411015T2 publication Critical patent/DE69411015T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/803—Physical recovery methods, e.g. chromatography, grinding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/804—Single cell protein
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/808—Materials and products related to genetic engineering or hybrid or fused cell technology, e.g. hybridoma, monoclonal products

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Endocrinology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Diabetes (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE69411015T 1993-08-20 1994-08-10 Waessrige mehrphasige isolierung eines polypeptids Expired - Lifetime DE69411015T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/110,663 US5407810A (en) 1993-08-20 1993-08-20 Aqueous multiple-phase isolation of polypeptide
PCT/US1994/009089 WO1995006059A1 (en) 1993-08-20 1994-08-10 Aqueous multiple-phase isolation of polypeptide

Publications (2)

Publication Number Publication Date
DE69411015D1 DE69411015D1 (de) 1998-07-16
DE69411015T2 true DE69411015T2 (de) 1998-10-22

Family

ID=22334217

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69411015T Expired - Lifetime DE69411015T2 (de) 1993-08-20 1994-08-10 Waessrige mehrphasige isolierung eines polypeptids

Country Status (11)

Country Link
US (3) US5407810A (de)
EP (1) EP0714403B1 (de)
JP (1) JP3646195B2 (de)
AT (1) ATE167193T1 (de)
AU (1) AU673624B2 (de)
CA (1) CA2167910C (de)
DE (1) DE69411015T2 (de)
DK (1) DK0714403T3 (de)
ES (1) ES2119222T3 (de)
GR (1) GR3027656T3 (de)
WO (1) WO1995006059A1 (de)

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US5407810A (en) * 1993-08-20 1995-04-18 Genentech, Inc. Aqueous multiple-phase isolation of polypeptide
TW517059B (en) * 1994-07-25 2003-01-11 Ciba Geigy Ag New process for the production of biologically active protein
AU3151495A (en) * 1994-08-01 1996-03-04 James T Hsu Lipoprotein cholesterol assays
WO1996007744A1 (en) * 1994-09-08 1996-03-14 Chiron Corporation A method of improved production of insulin-like growth factor
GB9618952D0 (en) 1996-09-11 1996-10-23 Sandoz Ltd Process
US6756484B1 (en) 1995-04-14 2004-06-29 Cephalon, Inc. IGF-I purification process
US5650496A (en) * 1995-04-14 1997-07-22 Cephalon, Inc. IGF-I purification process
US5789213A (en) * 1995-05-11 1998-08-04 Health Research Inc. Method and compositions for high efficiency loading, transfection and fusion of cells by electric pulses
US7071313B1 (en) 1995-06-07 2006-07-04 Cephalon, Inc. Methods of purifying authentic IGF from yeast hosts
JP2002514890A (ja) * 1995-06-07 2002-05-21 カイロン コーポレイション 酵母宿主から真性のｉｇｆを精製するための方法
US7193042B1 (en) 1995-06-07 2007-03-20 Chiron Corporation Methods for purifying authentic IGF from yeast hosts
ES2285632T3 (es) * 1995-06-07 2007-11-16 Novartis Vaccines And Diagnostics, Inc. Formulacion acuosa que comprende tfpi y agentes solubilizadores.
US5945515A (en) * 1995-07-31 1999-08-31 Chomczynski; Piotr Product and process for isolating DNA, RNA and proteins
SE9504019D0 (sv) * 1995-11-13 1995-11-13 Pharmacia Ab Method for production of proteins
US5734024A (en) * 1996-04-19 1998-03-31 Boris Y. Zaslavsky Method for determining the biological activity of recombinant human growth hormone
IT1291362B1 (it) * 1997-05-13 1999-01-07 Vectorpharma Int Composizioni farmaceutiche multicomponente bifasiche contenenti sostanze atte a modificare la partizione dei principi attivi
WO2000010674A1 (en) * 1997-08-19 2000-03-02 Arnon Chait Method for evaluation of the ratio of amounts of biomolecules or their sub-populations in a mixture
US20020052026A1 (en) * 1997-10-08 2002-05-02 Steven M. Vicik Methods of refolding proteins
US6265542B1 (en) 1997-10-24 2001-07-24 Genentech, Inc. Purification of molecules
AU5209399A (en) 1998-07-10 2000-02-01 Cornell Research Foundation Inc. Recombinant constructs and systems for secretion of proteins via type iii secretion systems
DK1115863T3 (da) 1998-09-22 2008-03-31 Genentech Inc Ucp4
JP2002533062A (ja) * 1998-11-30 2002-10-08 ジェネンテック・インコーポレーテッド Ｕｃｐ５
US7342102B2 (en) 1998-11-30 2008-03-11 Genentech, Inc. Uncoupling protein 5 (UCP5)
AU1891000A (en) * 1999-01-07 2000-07-24 Takeda Chemical Industries Ltd. Process for producing activated protein
US6437101B1 (en) 1999-05-07 2002-08-20 Akzo Nobel N.V. Methods for protein purification using aqueous two-phase extraction
US7229789B1 (en) * 2001-01-19 2007-06-12 N.V. Organon Methods and compositions for extracting proteins from cells
HUP0500733A3 (en) 2001-02-09 2010-01-28 Genentech Inc Crystallization of igf-1
EP1314739A1 (de) * 2001-11-22 2003-05-28 Bayer Ag Verfahren zur Renaturierung rekombinanter, Disulfid-enthaltender Proteine bei hohen Proteinkonzentrationen in Gegenwart von Aminen
US7998705B2 (en) * 2002-08-06 2011-08-16 FUJIFILM Diosynth Biotechnologies U.S.A., Inc Increased dynamic binding capacity in ion exchange chromatography by addition of polyethylene glycol
US6924124B1 (en) * 2002-08-23 2005-08-02 Immunex Corporation Feeding strategies for cell culture
US7465562B2 (en) * 2003-05-19 2008-12-16 Brandeis University Nucleic acid processing methods, kits and devices
US8415302B2 (en) 2004-01-28 2013-04-09 The Regents Of The University Of California Surgical applications for BMP binding protein
US8193312B2 (en) 2004-01-28 2012-06-05 The Regents Of The University Of California Bone morphogenic protein binding peptide
US8188219B2 (en) 2004-01-28 2012-05-29 The Regents Of The University Of California Bone morphogenic protein binding peptide
DE102004015965A1 (de) * 2004-04-01 2005-10-20 Aventis Pharma Gmbh Verfahren zur Gewinnung von Insulinen durch verbesserte Luftbegasung der Faltung
EP1761552B1 (de) * 2004-06-29 2008-09-17 Ares Trading S.A. Verfahren zur aufreinigung von il-18-bindendem protein
AU2005328325B2 (en) * 2004-11-29 2012-04-05 Department Of Veterans Affairs Activating extraction of demineralized bone matrix
EP1916997B1 (de) * 2005-08-05 2018-04-18 Amgen Inc. Pharmazeutische formulierungen aus stabilen wässrigen proteinen und ihre herstellung
US20070048786A1 (en) * 2005-09-01 2007-03-01 Arnon Chait Systems and methods for fractionation of protein mixtures
EP2445512B1 (de) 2009-06-23 2018-08-29 The Regents of The University of California Verbesserung der bmp-aufrechterhaltung
JP2017530702A (ja) * 2014-09-26 2017-10-19 ザイレコ，インコーポレイテッド 可溶化された酵素およびその使用
WO2016054375A1 (en) * 2014-10-01 2016-04-07 Impossible Foods Inc. Methods for extracting and purifying non-denatured proteins
JP2016172707A (ja) * 2015-03-18 2016-09-29 株式会社ＵｎｉＢｉｏ 植物体内で発現させた上皮細胞増殖因子の精製品の製造方法
US11051532B2 (en) 2017-09-22 2021-07-06 Impossible Foods Inc. Methods for purifying protein
US12011016B2 (en) 2020-09-14 2024-06-18 Impossible Foods Inc. Protein methods and compositions

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US4565785A (en) * 1978-06-08 1986-01-21 The President And Fellows Of Harvard College Recombinant DNA molecule
DE2943016C2 (de) * 1979-10-24 1984-09-06 Dr. Rentschler Arzneimittel Gmbh & Co, 7958 Laupheim Verfahren zur Reinigung von Interferon
JPS5815989A (ja) * 1981-06-12 1983-01-29 Sanki Eng Kk 生理活性蛋白質の精製方法
US4673641A (en) * 1982-12-16 1987-06-16 Molecular Genetics Research And Development Limited Partnership Co-aggregate purification of proteins
SE8302483L (sv) * 1983-05-02 1984-11-03 Pharmacia Ind Forfarande vid rening av biologiskt aktiva substanser
US4710473A (en) * 1983-08-10 1987-12-01 Amgen, Inc. DNA plasmids
US4738921A (en) * 1984-09-27 1988-04-19 Eli Lilly And Company Derivative of the tryptophan operon for expression of fused gene products
US4795706A (en) * 1985-01-31 1989-01-03 Eli Lilly And Company Novel expression control sequences
US4690892A (en) * 1985-04-29 1987-09-01 Union Carbide Corporation Process for the recycle of phase-forming liquids of multi-phase aqueous systems
DE3527268A1 (de) * 1985-07-30 1987-02-05 Biotechnolog Forschung Gmbh Verfahren zur reinigung und gegebenenfalls gewinnung von formiat-dehydrogenase (fdh) aus candida boidinii und fdh-haltiges produkt
US4684723A (en) * 1985-09-11 1987-08-04 Miles Laboratories, Inc. Method of separating proteins from aqueous solutions
IL86090A (en) * 1987-04-16 1993-03-15 Cetus Oncology Corp Production of purified, biologically active, bacterially produced recombinant human csf-1
US4961969A (en) * 1987-05-11 1990-10-09 Cetus Corporation Process for recovering microbially produced interferon-β
US4843155A (en) * 1987-11-19 1989-06-27 Piotr Chomczynski Product and process for isolating RNA
DD288837A5 (de) * 1988-11-08 1991-04-11 Zi Fuer Mikrobiologie Und Experimentelle Therapie,De Verfahren zur anreicherung rekombinanter proteine
US5124444A (en) * 1989-07-24 1992-06-23 Microprobe Corporation Lactam-containing compositions and methods useful for the extraction of nucleic acids
US5158875A (en) * 1989-08-25 1992-10-27 Amgen Inc. Production of biologically active insulin-like growth factor i from high expression host cell systems
DD298424A5 (de) * 1989-11-22 1992-02-20 Universitaet Leipzig,De Verfahren zur gewinnung von alkalischer phosphatase aus kaelberdarm
GB8927008D0 (en) * 1989-11-29 1990-01-17 Ciba Geigy Novel process for the production of unfused protein in e.coli
US5093254A (en) * 1990-01-23 1992-03-03 The United States Of America, As Represented By The Secretary Of Commerce Aqueous two-phase protein extraction
JPH06500084A (ja) * 1990-08-20 1994-01-06 ノボ　ノルディスク　アクティーゼルスカブ 生物学的に活性な化合物、その製造方法及びその利用
SE9003534D0 (sv) * 1990-11-06 1990-11-06 Kabigen Ab A method for isolating and purifying peptides and proteins
US5288931A (en) * 1991-12-06 1994-02-22 Genentech, Inc. Method for refolding insoluble, misfolded insulin-like growth factor-I into an active conformation
US5407810A (en) * 1993-08-20 1995-04-18 Genentech, Inc. Aqueous multiple-phase isolation of polypeptide

Also Published As

Publication number Publication date
WO1995006059A1 (en) 1995-03-02
EP0714403A1 (de) 1996-06-05
JP3646195B2 (ja) 2005-05-11
GR3027656T3 (en) 1998-11-30
EP0714403B1 (de) 1998-06-10
US5723310A (en) 1998-03-03
JPH09501931A (ja) 1997-02-25
CA2167910A1 (en) 1995-03-02
ES2119222T3 (es) 1998-10-01
AU673624B2 (en) 1996-11-14
US5695958A (en) 1997-12-09
ATE167193T1 (de) 1998-06-15
US5407810A (en) 1995-04-18
CA2167910C (en) 2009-10-06
DE69411015D1 (de) 1998-07-16
DK0714403T3 (da) 1999-03-22
AU7561694A (en) 1995-03-21

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