ES2061632T5 - Kluyveromyces como celula hospedante. - Google Patents

Kluyveromyces como celula hospedante.

Info

Publication number
ES2061632T5
ES2061632T5 ES88201632T ES88201632T ES2061632T5 ES 2061632 T5 ES2061632 T5 ES 2061632T5 ES 88201632 T ES88201632 T ES 88201632T ES 88201632 T ES88201632 T ES 88201632T ES 2061632 T5 ES2061632 T5 ES 2061632T5
Authority
ES
Spain
Prior art keywords
kluyveromyces
endogenous
product
hospitalizing
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES88201632T
Other languages
English (en)
Other versions
ES2061632T3 (es
Inventor
Den Berg Johannes A Dr Van
Ooyen Albert J J Dr Van
Krijn Dr Rietveld
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Koninklijke DSM NV
Original Assignee
DSM NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22144683&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2061632(T5) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from US07/078,539 external-priority patent/US4943529A/en
Application filed by DSM NV filed Critical DSM NV
Application granted granted Critical
Publication of ES2061632T3 publication Critical patent/ES2061632T3/es
Publication of ES2061632T5 publication Critical patent/ES2061632T5/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76—Albumins
    • C07K14/765—Serum albumin, e.g. HSA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01108—Lactase (3.2.1.108)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

LA INVENCION PROPORCIONA HUESPEDES DE KLUYVEROMYCES Y CASETTES DE EXPRESION DE DNA PARA SU EMPLEO EN KLUYVEROMICES PARA LA TRANSCRIPCION DE DNA ENDOGENO Y/O EXOGENO Y PRODUCCION DE PEPTIDOS, PARA MEJORAR LA PRODUCCION DE UN PRODUCTO ENDOGENO O PARA PRODUCIR UN PRODUCTO EXOGENO. LOS HUESPEDES DE KLUYVEROMYCES SON PARTICULARMENTE UTILES PARA LA SECRECION DE UN PRODUCTO PEPTIDICO DESEADO, EN LAS QUE LAS SECUENCIAS SEÑAL PUEDEN SER NATIVAS DEL PEPTIDO O SER PROPORCIONADAS A PARTIR DE SECUENCIAS SEÑAL ENDOGENAS O EXOGENAS, QUE INCLUYEN SECUENCIAS SINTETICAS, FUNCIONALES EN LOS KLUYVEROMYCES. LE PROPORCIONA TAMBIEN UN PROCEDIMIENTO PARA TRANSFORMAR KLUYVEROMYCES DE FORMA EFICAZ.
ES88201632T 1987-07-28 1988-07-28 Kluyveromyces como celula hospedante. Expired - Lifetime ES2061632T5 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/078,539 US4943529A (en) 1982-05-19 1987-07-28 Kluyveromyces as a host strain

Publications (2)

Publication Number Publication Date
ES2061632T3 ES2061632T3 (es) 1994-12-16
ES2061632T5 true ES2061632T5 (es) 2000-12-16

Family

ID=22144683

Family Applications (1)

Application Number Title Priority Date Filing Date
ES88201632T Expired - Lifetime ES2061632T5 (es) 1987-07-28 1988-07-28 Kluyveromyces como celula hospedante.

Country Status (20)

Country Link
EP (1) EP0301670B2 (es)
JP (1) JPH02476A (es)
KR (1) KR970007024B1 (es)
CN (1) CN1026994C (es)
AR (1) AR247761A1 (es)
AT (1) ATE97446T1 (es)
AU (1) AU628018B2 (es)
CA (1) CA1323850C (es)
DE (1) DE3885668T3 (es)
DK (1) DK175203B1 (es)
ES (1) ES2061632T5 (es)
FI (1) FI107939B (es)
GR (1) GR3034358T3 (es)
HU (1) HU208553B (es)
IE (1) IE62458B1 (es)
IL (1) IL87258A (es)
NO (1) NO302485B1 (es)
NZ (1) NZ225597A (es)
PT (1) PT88133B (es)
ZA (1) ZA885536B (es)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5010182A (en) * 1987-07-28 1991-04-23 Chiron Corporation DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides
FR2649991B2 (fr) * 1988-08-05 1994-03-04 Rhone Poulenc Sante Utilisation de derives stables du plasmide pkd1 pour l'expression et la secretion de proteines heterologues dans les levures du genre kluyveromyces
CA2017176A1 (en) * 1989-05-22 1990-11-22 Yoshitomi Pharmaceutical Industries Ltd. Albumin gene-containing plasmid, transformant carrying same, production of such transformant and production of albumin
FR2650598B1 (fr) * 1989-08-03 1994-06-03 Rhone Poulenc Sante Derives de l'albumine a fonction therapeutique
US5326700A (en) * 1990-11-06 1994-07-05 Eli Lilly And Company DNA sequences encoding t-PA derivatives with cleavable sites
FR2677996B1 (fr) * 1991-06-21 1993-08-27 Rhone Poulenc Rorer Sa Vecteurs de clonage et/ou d'expression preparation et utilisation.
FR2678636A1 (fr) * 1991-07-02 1993-01-08 Rhone Poulenc Rorer Sa Procede de production de proteines recombinantes et cellules hote utilisees.
IT1251435B (it) * 1991-09-04 1995-05-09 Sclavo Spa Vettore genetico per la integrazione multipla stabile di sequenze di dna nel genoma dei lieviti kluyveromyces lactis e saccharomyces cerevisiae e plasmidi che lo contengono.
FR2686899B1 (fr) 1992-01-31 1995-09-01 Rhone Poulenc Rorer Sa Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant.
FR2692907B1 (fr) * 1992-06-25 1995-06-30 Rhone Poulenc Rorer Sa Levures kluyveromyces modifiees, preparation et utilisation.
FR2693475B1 (fr) * 1992-07-08 1994-09-02 Rhone Poulenc Rorer Sa Procédé d'identification et/ou de clonage de promoteurs transcriptionnels, et utilisation de ces promoteurs pour l'expression de gènes.
GB9526733D0 (en) 1995-12-30 1996-02-28 Delta Biotechnology Ltd Fusion proteins
GB9907461D0 (en) 1999-03-31 1999-05-26 King S College London Neurite regeneration
US6265186B1 (en) 1997-04-11 2001-07-24 Dsm N.V. Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces
CN1059236C (zh) * 1997-04-24 2000-12-06 复旦大学 脆壁克鲁维酵母的高效转化系统
US8372620B2 (en) 1998-12-23 2013-02-12 Dupont Nutrition Biosciences Aps Bacterial xylanases
CA2405557C (en) 2000-04-12 2013-09-24 Human Genome Sciences, Inc. Albumin fusion proteins
US6946134B1 (en) 2000-04-12 2005-09-20 Human Genome Sciences, Inc. Albumin fusion proteins
GB0020498D0 (en) 2000-08-18 2000-10-11 Sterix Ltd Compound
AU2000277798B2 (en) 2000-09-21 2006-06-22 Basf Aktiengesellschaft Talaromyces xylanases
CA2706364A1 (en) 2000-12-07 2002-06-13 Dsm Ip Assets B.V. Method for the prevention or reduction of haze in beverages
DK1339837T3 (da) 2000-12-07 2008-06-09 Dsm Ip Assets Bv Prolyl-endoprotease fra Aspergillus Niger
GB0116453D0 (en) 2001-07-05 2001-08-29 Imp College Innovations Ltd Method
CN101724075B (zh) 2001-10-10 2014-04-30 诺和诺德公司 肽的重构和糖缀合
EP2292273A3 (en) 2001-10-10 2011-11-23 BioGeneriX AG Remodeling and glycoconjugation of peptides
WO2004099231A2 (en) 2003-04-09 2004-11-18 Neose Technologies, Inc. Glycopegylation methods and proteins/peptides produced by the methods
EP2261250B1 (en) 2001-12-21 2015-07-01 Human Genome Sciences, Inc. GCSF-Albumin fusion proteins
EP1511835B9 (en) 2002-06-07 2015-02-25 DSM IP Assets B.V. Improved method for the prevention or reduction of haze in beverages
TWI328612B (en) 2002-07-25 2010-08-11 Dsm Ip Assets Bv Genes and their encoded polypeptides involved in the biosynthetic pathway of vitamin b12, vectors and host cells comprising the genes, and process for producing vitamin b12
GB0403864D0 (en) 2004-02-20 2004-03-24 Ucl Ventures Modulator
ES2246175B1 (es) * 2004-07-30 2007-03-16 Felix Claverie Martin "procedimiento para producir quimosina caprina recombinante para la coagulacion de la leche de cabra en la fabricacion de queso".
WO2007060247A2 (en) 2005-11-28 2007-05-31 Dsm Ip Assets B.V. Enzyme preparations yielding a clean taste
GB0600406D0 (en) 2006-01-10 2006-02-15 Univ Bath Crystal
EP1816201A1 (en) 2006-02-06 2007-08-08 CSL Behring GmbH Modified coagulation factor VIIa with extended half-life
NZ571699A (en) 2006-04-13 2011-07-29 Dsm Ip Assets Bv Liquid composition comprising an aspartic protease
NZ578882A (en) 2007-02-20 2012-01-12 Dsm Ip Assets Bv Sialidase from the fungus Penicilium chrysogenum
ES2608033T3 (es) 2007-03-22 2017-04-05 Dsm Ip Assets B.V. Nuevas lisil oxidasas
JP5435657B2 (ja) * 2008-03-18 2014-03-05 国立大学法人山口大学 凝集性酵母、及びその作製法
WO2011101339A2 (en) 2010-02-18 2011-08-25 Dsm Ip Assets B.V. Fermentation broth and filtration filter cake and uses thereof
WO2012007445A1 (en) 2010-07-13 2012-01-19 Dsm Ip Assets B.V. Mutarotase in crystallization
WO2012007446A1 (en) 2010-07-13 2012-01-19 Dsm Ip Assets B.V. Use of a mutarotase in the production of dried powders
CN115806889B (zh) * 2022-09-28 2024-06-11 山东大学 一种提高基因表达水平的酿酒酵母工程菌及其构建方法与应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL66614A (en) * 1981-08-28 1985-09-29 Genentech Inc Method of constructing a dna sequence encoding a polypeptide,microbial production of human serum albumin,and pharmaceutical compositions comprising it
MX197183A (es) * 1982-05-05 1994-02-28 Genentech Inc Activador de plasminogeno de tejido humano
EP0096910B1 (en) * 1982-05-19 1989-04-19 Unilever N.V. Yeast of the genus kluyveromyces modified for the expression of preprothaumatin or its various allelic and modified forms or their maturation forms
DE3382547D1 (de) * 1983-01-12 1992-05-27 Chiron Corp Sekretorische expression in eukaryoten.
IT1203758B (it) * 1986-03-27 1989-02-23 Univ Roma Vettori di clonazione e di espressione di geni eterologhi in lieviti e lieviti trasformati con tali vettori

Also Published As

Publication number Publication date
KR970007024B1 (ko) 1997-05-02
AU2014888A (en) 1989-03-02
EP0301670A1 (en) 1989-02-01
CA1323850C (en) 1993-11-02
AR247761A1 (es) 1995-03-31
IL87258A (en) 1994-02-27
IE62458B1 (en) 1995-02-08
PT88133B (pt) 1995-03-01
NZ225597A (en) 1991-08-27
DK422188A (da) 1989-01-29
IL87258A0 (en) 1988-12-30
FI883540A0 (fi) 1988-07-28
DK175203B1 (da) 2004-07-05
DE3885668T3 (de) 2000-08-17
HUT47637A (en) 1989-03-28
GR3034358T3 (en) 2000-12-29
KR890002407A (ko) 1989-04-10
EP0301670B1 (en) 1993-11-18
JPH02476A (ja) 1990-01-05
NO302485B1 (no) 1998-03-09
NO883347D0 (no) 1988-07-28
FI107939B (fi) 2001-10-31
FI883540A (fi) 1989-01-29
IE882314L (en) 1989-01-28
DE3885668D1 (de) 1993-12-23
ATE97446T1 (de) 1993-12-15
DK422188D0 (da) 1988-07-28
NO883347L (no) 1989-01-30
AU628018B2 (en) 1992-09-10
ES2061632T3 (es) 1994-12-16
CN1040052A (zh) 1990-02-28
HU208553B (en) 1993-11-29
DE3885668T2 (de) 1994-05-05
ZA885536B (en) 1989-04-26
EP0301670B2 (en) 2000-06-14
PT88133A (pt) 1989-06-30
CN1026994C (zh) 1994-12-14

Similar Documents

Publication Publication Date Title
ES2061632T5 (es) Kluyveromyces como celula hospedante.
DK0471754T3 (da) Produktion af vaskulær endotelcelle vækstfaktor, samt DNA kodende for denne
ES2127164T1 (es) Expresion de lactoferrina recombinante y fragmentos polipeptidicos de lactoferrina procesados a partir de un producto de fusion en aspergillus.
ES2094111T3 (es) Proteinas con actividad de factor viii, procedimiento para su produccion que utilizan celulas modificadas por ingenieria genetica y composiciones farmaceuticas que las contienen.
DE69323879D1 (de) Herstellung von rekombinanten, menschlichem lactoferrin
AR000610A1 (es) Un método para la expresión de un polipéptido recombinante por una célula huésped
ES2054878T3 (es) Expresion de la proapolipoproteina a-i humana.
DE69018920D1 (de) Verwendung von dns-fragmenten, die ein signalpeptid kodieren zur sekretion von reifen proteinen in rekombinanten hefen, expressions-kassetten, transformierte hefen und verfahren zur herstellung dieser proteine.
NO882946L (no) Konstruksjon av syntetisk dna og anvendelse derav i syntese av store polypeptider.
ATE195148T1 (de) Prozess zur rekombinanten herstellung von hiv-2 - proteinen sowie zellen, die hiv-2 - proteine exprimiert
DE60044690D1 (de) Verfahren zur Herstellung von rekombinanten Proteinen mittels Inhibitoren von Apoptose
ATE173023T1 (de) Dna-sequenzen kodierend für das polypeptid gelonin
AR024335A1 (es) Hialuronidasa de hirudinaria manillensis, aislamiento, purificacion y metodo recombinante de produccion
CH491864A (de) Verfahren zur Herstellung biologisch aktiver Peptide
ES2125864T3 (es) Vectores bacterianos.
AR241794A1 (es) Procedimiento para obtener inteerleucina-2 humana, en una celula de ovario de hamster de la china.
ES2059436T3 (es) Proteina de la membrana exterior f de pseudomonas aeruginosa.
IT1212508B (it) Analoghi retro - invertiti dei penta - ed esapeptidi c - terminali della sostanza p. utili come vasodilatatori
ES2058486T3 (es) Inmunogenos nativos y recombinantes del grupo b de eimeria tenella utiles como vacunas para las coccidiosis.
HUT65369A (en) Bcrf1 proteins as inhibitors of gamma-interferon
ES8704184A1 (es) Un metodo para producir un polipeptidos similar a lipocortina humana.
JPS56127315A (en) Method for obtaining human relaxin
AR214337A1 (es) Metodo para obtener micelios en elevados rendimientos medioriolus de polyporaceae ante el cultivo de basidiomycete perteneciente al genero c
AR040521A1 (es) Gen sisntetico que codifica al factor estimulante de colonias de granulocitos humano para la expresion e. coli
ES2061524T3 (es) Un vector plasmidico para la expresion en bacillus y empleado para la clonacion del gen estructural que codifica la hormona de crecimiento humana y un metodo para la produccion de la hormona.

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 301670

Country of ref document: ES