AR024335A1 - Hialuronidasa de hirudinaria manillensis, aislamiento, purificacion y metodo recombinante de produccion - Google Patents
Hialuronidasa de hirudinaria manillensis, aislamiento, purificacion y metodo recombinante de produccionInfo
- Publication number
- AR024335A1 AR024335A1 ARP000102882A ARP000102882A AR024335A1 AR 024335 A1 AR024335 A1 AR 024335A1 AR P000102882 A ARP000102882 A AR P000102882A AR P000102882 A ARP000102882 A AR P000102882A AR 024335 A1 AR024335 A1 AR 024335A1
- Authority
- AR
- Argentina
- Prior art keywords
- purification
- production
- manilase
- hilluronidase
- insulation
- Prior art date
Links
- 241000500851 Poecilobdella manillensis Species 0.000 title abstract 2
- 238000004519 manufacturing process Methods 0.000 title abstract 2
- 238000000746 purification Methods 0.000 title abstract 2
- 238000009413 insulation Methods 0.000 title 1
- 238000010188 recombinant method Methods 0.000 title 1
- 208000031229 Cardiomyopathies Diseases 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 108010003272 Hyaluronate lyase Proteins 0.000 abstract 1
- 102000001974 Hyaluronidases Human genes 0.000 abstract 1
- 206010028980 Neoplasm Diseases 0.000 abstract 1
- 208000007536 Thrombosis Diseases 0.000 abstract 1
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 238000012512 characterization method Methods 0.000 abstract 1
- 229940088598 enzyme Drugs 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 229960002773 hyaluronidase Drugs 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 230000001225 therapeutic effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Public Health (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Veterinary Medicine (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Cardiology (AREA)
- Heart & Thoracic Surgery (AREA)
- Diabetes (AREA)
- Hematology (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Aislamiento, la purificacion y caracterizacion de una nueva hialuronidasa que deriva de la sanguijuela tropical Hirudinaria manillensis. Por lo tanto, deacuerdo con la presente la nueva enzima se llamo manilasa. Se relaciona además con el métodoreco mbinante de produccion de manilasa que incluye larevelacion de secuencias de ADN y de aminoácidos así como vectores de expresion y sistemas de hospedantes. Finalmente, el uso de la manilasa para finesterapéuticos, por ejemplo, para eltratamiento de enfermedades del miocardio, eventos tromboticos y tumores.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP99111468 | 1999-06-12 |
Publications (1)
Publication Number | Publication Date |
---|---|
AR024335A1 true AR024335A1 (es) | 2002-10-02 |
Family
ID=8238352
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP000102882A AR024335A1 (es) | 1999-06-12 | 2000-06-12 | Hialuronidasa de hirudinaria manillensis, aislamiento, purificacion y metodo recombinante de produccion |
Country Status (23)
Country | Link |
---|---|
US (1) | US7049124B1 (es) |
EP (1) | EP1185666B1 (es) |
JP (1) | JP2003502045A (es) |
KR (1) | KR20020011138A (es) |
CN (1) | CN1355850A (es) |
AR (1) | AR024335A1 (es) |
AT (1) | ATE324452T1 (es) |
AU (1) | AU6149300A (es) |
BR (1) | BR0011500A (es) |
CA (1) | CA2376467A1 (es) |
CZ (1) | CZ20014383A3 (es) |
DE (1) | DE60027562T2 (es) |
DK (1) | DK1185666T3 (es) |
ES (1) | ES2258976T3 (es) |
HK (1) | HK1046297A1 (es) |
HU (1) | HUP0201452A2 (es) |
MX (1) | MXPA01012806A (es) |
NO (1) | NO20016047L (es) |
PL (1) | PL352060A1 (es) |
PT (1) | PT1185666E (es) |
SK (1) | SK17612001A3 (es) |
WO (1) | WO2000077221A1 (es) |
ZA (1) | ZA200200233B (es) |
Families Citing this family (25)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6610292B2 (en) | 1995-11-22 | 2003-08-26 | Ista Pharmaceuticals, Inc. | Use of hyaluronidase in the manufacture of an ophthalmic preparation for liquefying vitreous humor in the treatment of eye disorders |
US5866120A (en) | 1995-11-22 | 1999-02-02 | Advanced Corneal Systems, Inc. | Method for accelerating clearance of hemorrhagic blood from the vitreous humor with hyaluronidase |
JP2002506013A (ja) | 1998-03-09 | 2002-02-26 | イスタ ファーマシューティカルズ インコーポレイテッド | 酵素角膜矯正術における角膜硬化剤の使用 |
WO2004092361A1 (en) * | 2003-04-15 | 2004-10-28 | Ista Pharmaceuticals, Inc. | Process for isolating and purifing ovine hyaluronidase |
AU2003239763A1 (en) * | 2003-05-27 | 2005-01-04 | Friedrich-Schiller- Universität Jena | Hyaluronate lyase-containing agent for treating acute myocardial infarction or symptoms thereof and for the treatment of postinfarction myocarditis and myocardial ischaemia |
WO2005030962A1 (en) * | 2003-09-26 | 2005-04-07 | Istituto Di Ricerche Di Biologia Molecolare P Angeletti Spa | Synthetic heparanase molecules and uses thereof |
US8288142B2 (en) * | 2007-06-19 | 2012-10-16 | Uvarkina Tamara P | Hyaluronidase and method of use thereof |
GB0717166D0 (en) * | 2007-09-04 | 2007-10-17 | Univ Brighton | Method for stabilisation of purified P-Glycoprotein |
KR20140130512A (ko) * | 2008-03-06 | 2014-11-10 | 할로자임, 아이엔씨 | 가용성 히알루로니다아제의 대규모 제조 |
EP2177227B1 (en) * | 2008-10-14 | 2013-03-20 | Burgard, Gunter, Dr. med. | Use of hyaluronidase for the prevention or treatment of arterial hypertension. |
WO2013057171A1 (en) * | 2011-10-18 | 2013-04-25 | Csl Behring Gmbh | Combined use of a sulfated glycosaminoglycan and a hyaluronidase for improving the bioavailability of factor viii |
EP2698636A1 (en) * | 2012-08-13 | 2014-02-19 | Fundació Institut d'Investigació Biomèdica de Bellvitge | Methods and reagents for prevention and/or treatment of transplant rejection |
US8784896B2 (en) | 2012-09-17 | 2014-07-22 | Biopep Solutions, Inc | Antioxidant therapy with a whole, leech saliva extract |
KR101454646B1 (ko) | 2012-11-05 | 2014-10-27 | (주)한국비엠아이 | 히알루로니다아제의 안정화 제제 및 이를 포함하는 액상제제 |
CN103060291B (zh) * | 2012-12-28 | 2014-10-08 | 青岛九龙生物医药有限公司 | 一种玻璃酸酶的提取方法 |
CN103695448B (zh) * | 2013-07-29 | 2016-08-17 | 江南大学 | 一种透明质酸酶编码基因及其发酵生产和纯化方法 |
US9279111B2 (en) * | 2013-07-29 | 2016-03-08 | Jiangnan University | Leech hyaluronidase and its application |
CN104263666B (zh) * | 2014-09-12 | 2017-07-14 | 江南大学 | 一种产小分子透明质酸的重组毕赤酵母及其构建方法 |
CN106148302A (zh) * | 2016-07-08 | 2016-11-23 | 中国人民解放军军事医学科学院生物工程研究所 | 一种重组蛋白质的纯化方法 |
CN106520728B (zh) * | 2016-11-08 | 2019-06-21 | 江南大学 | 一种提高水蛭透明质酸酶酶活的方法 |
CN109298113B (zh) * | 2018-12-11 | 2021-06-18 | 华熙生物科技股份有限公司 | 一种测定包含有柠檬酸的溶液中的透明质酸含量的方法 |
EP4139448A1 (en) * | 2020-04-20 | 2023-03-01 | Pharmact Holding AG | A modified bacterial hyaluronidase polypeptide, production process, pharmaceutical compositions and their uses |
CN112915106A (zh) * | 2021-02-05 | 2021-06-08 | 张虎山 | 一种肿瘤免疫微环境调控剂的制备及其应用 |
WO2023067008A1 (en) * | 2021-10-19 | 2023-04-27 | Pharmact Holding Ag | A production of a purified modified bacterial hyaluronidase polypeptide, pharmaceutical compositions and their uses |
CN116640745B (zh) * | 2023-06-29 | 2023-12-08 | 江南大学 | 透明质酸酶突变体及其在水解硫酸软骨素中的应用 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB8504025D0 (en) * | 1985-02-16 | 1985-03-20 | Biopharm Ltd | Hyaluronidase |
DE4440892A1 (de) * | 1994-11-17 | 1996-05-23 | Gruenenthal Gmbh | Proteine mit fibrinolytischen und gerinnungshemmenden Eigenschaften |
US5747027A (en) * | 1995-04-07 | 1998-05-05 | The Regents Of The University Of California | BH55 hyaluronidase |
-
2000
- 2000-06-06 PL PL00352060A patent/PL352060A1/xx unknown
- 2000-06-06 CN CN00808873A patent/CN1355850A/zh active Pending
- 2000-06-06 CA CA002376467A patent/CA2376467A1/en not_active Abandoned
- 2000-06-06 KR KR1020017016004A patent/KR20020011138A/ko not_active Application Discontinuation
- 2000-06-06 MX MXPA01012806A patent/MXPA01012806A/es unknown
- 2000-06-06 EP EP00947835A patent/EP1185666B1/en not_active Expired - Lifetime
- 2000-06-06 DK DK00947835T patent/DK1185666T3/da active
- 2000-06-06 HU HU0201452A patent/HUP0201452A2/hu unknown
- 2000-06-06 DE DE60027562T patent/DE60027562T2/de not_active Expired - Fee Related
- 2000-06-06 AT AT00947835T patent/ATE324452T1/de not_active IP Right Cessation
- 2000-06-06 BR BR0011500-2A patent/BR0011500A/pt not_active IP Right Cessation
- 2000-06-06 PT PT00947835T patent/PT1185666E/pt unknown
- 2000-06-06 JP JP2001503663A patent/JP2003502045A/ja active Pending
- 2000-06-06 CZ CZ20014383A patent/CZ20014383A3/cs unknown
- 2000-06-06 WO PCT/EP2000/005181 patent/WO2000077221A1/en active IP Right Grant
- 2000-06-06 AU AU61493/00A patent/AU6149300A/en not_active Abandoned
- 2000-06-06 US US10/009,500 patent/US7049124B1/en not_active Expired - Fee Related
- 2000-06-06 ES ES00947835T patent/ES2258976T3/es not_active Expired - Lifetime
- 2000-06-06 SK SK1761-2001A patent/SK17612001A3/sk unknown
- 2000-06-12 AR ARP000102882A patent/AR024335A1/es not_active Application Discontinuation
-
2001
- 2001-12-11 NO NO20016047A patent/NO20016047L/no not_active Application Discontinuation
-
2002
- 2002-01-10 ZA ZA200200233A patent/ZA200200233B/en unknown
- 2002-10-30 HK HK02107860.0A patent/HK1046297A1/zh unknown
Also Published As
Publication number | Publication date |
---|---|
NO20016047D0 (no) | 2001-12-11 |
DE60027562T2 (de) | 2006-11-02 |
US7049124B1 (en) | 2006-05-23 |
DE60027562D1 (de) | 2006-06-01 |
PL352060A1 (en) | 2003-07-28 |
CZ20014383A3 (cs) | 2002-03-13 |
HK1046297A1 (zh) | 2003-01-03 |
ATE324452T1 (de) | 2006-05-15 |
CA2376467A1 (en) | 2000-12-21 |
ES2258976T3 (es) | 2006-09-16 |
SK17612001A3 (sk) | 2002-04-04 |
EP1185666A1 (en) | 2002-03-13 |
MXPA01012806A (es) | 2002-09-02 |
NO20016047L (no) | 2001-12-11 |
EP1185666B1 (en) | 2006-04-26 |
AU6149300A (en) | 2001-01-02 |
CN1355850A (zh) | 2002-06-26 |
KR20020011138A (ko) | 2002-02-07 |
WO2000077221A1 (en) | 2000-12-21 |
JP2003502045A (ja) | 2003-01-21 |
ZA200200233B (en) | 2003-06-25 |
BR0011500A (pt) | 2002-03-12 |
PT1185666E (pt) | 2006-09-29 |
DK1185666T3 (da) | 2006-07-03 |
HUP0201452A2 (en) | 2002-08-28 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FA | Abandonment or withdrawal |