BR0011500A - Hialuronidase de hirudinaria manillensis, isolamento, purificação e método recombinante de produção - Google Patents
Hialuronidase de hirudinaria manillensis, isolamento, purificação e método recombinante de produçãoInfo
- Publication number
- BR0011500A BR0011500A BR0011500-2A BR0011500A BR0011500A BR 0011500 A BR0011500 A BR 0011500A BR 0011500 A BR0011500 A BR 0011500A BR 0011500 A BR0011500 A BR 0011500A
- Authority
- BR
- Brazil
- Prior art keywords
- hyaluronidase
- isolation
- purification
- hirudinaria manillensis
- production method
- Prior art date
Links
- 108010003272 Hyaluronate lyase Proteins 0.000 title abstract 3
- 102000001974 Hyaluronidases Human genes 0.000 title abstract 3
- 241000500851 Poecilobdella manillensis Species 0.000 title abstract 3
- 229960002773 hyaluronidase Drugs 0.000 title abstract 3
- 238000002955 isolation Methods 0.000 title abstract 3
- 238000004519 manufacturing process Methods 0.000 title abstract 3
- 238000000746 purification Methods 0.000 title abstract 3
- 208000031229 Cardiomyopathies Diseases 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 206010028980 Neoplasm Diseases 0.000 abstract 1
- 208000007536 Thrombosis Diseases 0.000 abstract 1
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 238000012512 characterization method Methods 0.000 abstract 1
- 229940088598 enzyme Drugs 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 238000010188 recombinant method Methods 0.000 abstract 1
- 230000001225 therapeutic effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Public Health (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Veterinary Medicine (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Cardiology (AREA)
- Heart & Thoracic Surgery (AREA)
- Diabetes (AREA)
- Hematology (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Patente de Invenção: "HIALURONIDASE DE HIRUDINARIA MANILLENSIS, ISOLAMENTO, PURIFICAçãO E MéTODO RECOMBINANTE DE PRODUçãO". A presente invenção refere-se ao isolamento, purificação e caracterização de uma hialuronidase a qual deriva da sanguessuga tropical Hirudinaria manillensis. Portanto, de acordo com a presente invenção, a enzima foi denominada "manilase". A invenção refere-se, além disso, ao método recombinante de produção de manilase o qual inclui a divulgação de seq³ências de DNA e aminoácido, bem como de vetores de expressão e sistemas hospedeiros. Finalmente, a invenção refere-se ao uso de manilase para fins terapêuticos, por exemplo, para o tratamento de doenças miocárdicas, eventos trombóticos e tumores.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP99111468 | 1999-06-12 | ||
| PCT/EP2000/005181 WO2000077221A1 (en) | 1999-06-12 | 2000-06-06 | Hyaluronidase from the hirudinaria manillensis, isolation, purification and recombinant method of production |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR0011500A true BR0011500A (pt) | 2002-03-12 |
Family
ID=8238352
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR0011500-2A BR0011500A (pt) | 1999-06-12 | 2000-06-06 | Hialuronidase de hirudinaria manillensis, isolamento, purificação e método recombinante de produção |
Country Status (23)
| Country | Link |
|---|---|
| US (1) | US7049124B1 (pt) |
| EP (1) | EP1185666B1 (pt) |
| JP (1) | JP2003502045A (pt) |
| KR (1) | KR20020011138A (pt) |
| CN (1) | CN1355850A (pt) |
| AR (1) | AR024335A1 (pt) |
| AT (1) | ATE324452T1 (pt) |
| AU (1) | AU6149300A (pt) |
| BR (1) | BR0011500A (pt) |
| CA (1) | CA2376467A1 (pt) |
| CZ (1) | CZ20014383A3 (pt) |
| DE (1) | DE60027562T2 (pt) |
| DK (1) | DK1185666T3 (pt) |
| ES (1) | ES2258976T3 (pt) |
| HK (1) | HK1046297A1 (pt) |
| HU (1) | HUP0201452A2 (pt) |
| MX (1) | MXPA01012806A (pt) |
| NO (1) | NO20016047L (pt) |
| PL (1) | PL352060A1 (pt) |
| PT (1) | PT1185666E (pt) |
| SK (1) | SK17612001A3 (pt) |
| WO (1) | WO2000077221A1 (pt) |
| ZA (1) | ZA200200233B (pt) |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6610292B2 (en) | 1995-11-22 | 2003-08-26 | Ista Pharmaceuticals, Inc. | Use of hyaluronidase in the manufacture of an ophthalmic preparation for liquefying vitreous humor in the treatment of eye disorders |
| US5866120A (en) | 1995-11-22 | 1999-02-02 | Advanced Corneal Systems, Inc. | Method for accelerating clearance of hemorrhagic blood from the vitreous humor with hyaluronidase |
| JP2002506013A (ja) | 1998-03-09 | 2002-02-26 | イスタ ファーマシューティカルズ インコーポレイテッド | 酵素角膜矯正術における角膜硬化剤の使用 |
| WO2004092361A1 (en) * | 2003-04-15 | 2004-10-28 | Ista Pharmaceuticals, Inc. | Process for isolating and purifing ovine hyaluronidase |
| AU2003239763A1 (en) * | 2003-05-27 | 2005-01-04 | Friedrich-Schiller- Universität Jena | Hyaluronate lyase-containing agent for treating acute myocardial infarction or symptoms thereof and for the treatment of postinfarction myocarditis and myocardial ischaemia |
| WO2005030962A1 (en) * | 2003-09-26 | 2005-04-07 | Istituto Di Ricerche Di Biologia Molecolare P Angeletti Spa | Synthetic heparanase molecules and uses thereof |
| US8288142B2 (en) * | 2007-06-19 | 2012-10-16 | Uvarkina Tamara P | Hyaluronidase and method of use thereof |
| GB0717166D0 (en) * | 2007-09-04 | 2007-10-17 | Univ Brighton | Method for stabilisation of purified P-Glycoprotein |
| KR20140130512A (ko) * | 2008-03-06 | 2014-11-10 | 할로자임, 아이엔씨 | 가용성 히알루로니다아제의 대규모 제조 |
| EP2177227B1 (en) * | 2008-10-14 | 2013-03-20 | Burgard, Gunter, Dr. med. | Use of hyaluronidase for the prevention or treatment of arterial hypertension. |
| WO2013057171A1 (en) * | 2011-10-18 | 2013-04-25 | Csl Behring Gmbh | Combined use of a sulfated glycosaminoglycan and a hyaluronidase for improving the bioavailability of factor viii |
| EP2698636A1 (en) * | 2012-08-13 | 2014-02-19 | Fundació Institut d'Investigació Biomèdica de Bellvitge | Methods and reagents for prevention and/or treatment of transplant rejection |
| US8784896B2 (en) | 2012-09-17 | 2014-07-22 | Biopep Solutions, Inc | Antioxidant therapy with a whole, leech saliva extract |
| KR101454646B1 (ko) | 2012-11-05 | 2014-10-27 | (주)한국비엠아이 | 히알루로니다아제의 안정화 제제 및 이를 포함하는 액상제제 |
| CN103060291B (zh) * | 2012-12-28 | 2014-10-08 | 青岛九龙生物医药有限公司 | 一种玻璃酸酶的提取方法 |
| CN103695448B (zh) * | 2013-07-29 | 2016-08-17 | 江南大学 | 一种透明质酸酶编码基因及其发酵生产和纯化方法 |
| US9279111B2 (en) * | 2013-07-29 | 2016-03-08 | Jiangnan University | Leech hyaluronidase and its application |
| CN104263666B (zh) * | 2014-09-12 | 2017-07-14 | 江南大学 | 一种产小分子透明质酸的重组毕赤酵母及其构建方法 |
| CN106148302A (zh) * | 2016-07-08 | 2016-11-23 | 中国人民解放军军事医学科学院生物工程研究所 | 一种重组蛋白质的纯化方法 |
| CN106520728B (zh) * | 2016-11-08 | 2019-06-21 | 江南大学 | 一种提高水蛭透明质酸酶酶活的方法 |
| CN109298113B (zh) * | 2018-12-11 | 2021-06-18 | 华熙生物科技股份有限公司 | 一种测定包含有柠檬酸的溶液中的透明质酸含量的方法 |
| EP4139448A1 (en) * | 2020-04-20 | 2023-03-01 | Pharmact Holding AG | A modified bacterial hyaluronidase polypeptide, production process, pharmaceutical compositions and their uses |
| CN112915106A (zh) * | 2021-02-05 | 2021-06-08 | 张虎山 | 一种肿瘤免疫微环境调控剂的制备及其应用 |
| WO2023067008A1 (en) * | 2021-10-19 | 2023-04-27 | Pharmact Holding Ag | A production of a purified modified bacterial hyaluronidase polypeptide, pharmaceutical compositions and their uses |
| CN116640745B (zh) * | 2023-06-29 | 2023-12-08 | 江南大学 | 透明质酸酶突变体及其在水解硫酸软骨素中的应用 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8504025D0 (en) * | 1985-02-16 | 1985-03-20 | Biopharm Ltd | Hyaluronidase |
| DE4440892A1 (de) * | 1994-11-17 | 1996-05-23 | Gruenenthal Gmbh | Proteine mit fibrinolytischen und gerinnungshemmenden Eigenschaften |
| US5747027A (en) * | 1995-04-07 | 1998-05-05 | The Regents Of The University Of California | BH55 hyaluronidase |
-
2000
- 2000-06-06 PL PL00352060A patent/PL352060A1/xx unknown
- 2000-06-06 CN CN00808873A patent/CN1355850A/zh active Pending
- 2000-06-06 CA CA002376467A patent/CA2376467A1/en not_active Abandoned
- 2000-06-06 KR KR1020017016004A patent/KR20020011138A/ko not_active Application Discontinuation
- 2000-06-06 MX MXPA01012806A patent/MXPA01012806A/es unknown
- 2000-06-06 EP EP00947835A patent/EP1185666B1/en not_active Expired - Lifetime
- 2000-06-06 DK DK00947835T patent/DK1185666T3/da active
- 2000-06-06 HU HU0201452A patent/HUP0201452A2/hu unknown
- 2000-06-06 DE DE60027562T patent/DE60027562T2/de not_active Expired - Fee Related
- 2000-06-06 AT AT00947835T patent/ATE324452T1/de not_active IP Right Cessation
- 2000-06-06 BR BR0011500-2A patent/BR0011500A/pt not_active IP Right Cessation
- 2000-06-06 PT PT00947835T patent/PT1185666E/pt unknown
- 2000-06-06 JP JP2001503663A patent/JP2003502045A/ja active Pending
- 2000-06-06 CZ CZ20014383A patent/CZ20014383A3/cs unknown
- 2000-06-06 WO PCT/EP2000/005181 patent/WO2000077221A1/en active IP Right Grant
- 2000-06-06 AU AU61493/00A patent/AU6149300A/en not_active Abandoned
- 2000-06-06 US US10/009,500 patent/US7049124B1/en not_active Expired - Fee Related
- 2000-06-06 ES ES00947835T patent/ES2258976T3/es not_active Expired - Lifetime
- 2000-06-06 SK SK1761-2001A patent/SK17612001A3/sk unknown
- 2000-06-12 AR ARP000102882A patent/AR024335A1/es not_active Application Discontinuation
-
2001
- 2001-12-11 NO NO20016047A patent/NO20016047L/no not_active Application Discontinuation
-
2002
- 2002-01-10 ZA ZA200200233A patent/ZA200200233B/en unknown
- 2002-10-30 HK HK02107860.0A patent/HK1046297A1/zh unknown
Also Published As
| Publication number | Publication date |
|---|---|
| NO20016047D0 (no) | 2001-12-11 |
| DE60027562T2 (de) | 2006-11-02 |
| US7049124B1 (en) | 2006-05-23 |
| DE60027562D1 (de) | 2006-06-01 |
| PL352060A1 (en) | 2003-07-28 |
| CZ20014383A3 (cs) | 2002-03-13 |
| HK1046297A1 (zh) | 2003-01-03 |
| ATE324452T1 (de) | 2006-05-15 |
| CA2376467A1 (en) | 2000-12-21 |
| ES2258976T3 (es) | 2006-09-16 |
| SK17612001A3 (sk) | 2002-04-04 |
| EP1185666A1 (en) | 2002-03-13 |
| MXPA01012806A (es) | 2002-09-02 |
| NO20016047L (no) | 2001-12-11 |
| EP1185666B1 (en) | 2006-04-26 |
| AU6149300A (en) | 2001-01-02 |
| CN1355850A (zh) | 2002-06-26 |
| KR20020011138A (ko) | 2002-02-07 |
| AR024335A1 (es) | 2002-10-02 |
| WO2000077221A1 (en) | 2000-12-21 |
| JP2003502045A (ja) | 2003-01-21 |
| ZA200200233B (en) | 2003-06-25 |
| PT1185666E (pt) | 2006-09-29 |
| DK1185666T3 (da) | 2006-07-03 |
| HUP0201452A2 (en) | 2002-08-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE A 5A, 6A E 7A ANUIDADES. |
|
| B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 1927 DE 11/12/2007. |