ES2059401T3 - Procedimiento microbiologico para la obtencion de una proteina mediante cultivo de una cepa bacteriana mutante. - Google Patents
Procedimiento microbiologico para la obtencion de una proteina mediante cultivo de una cepa bacteriana mutante.Info
- Publication number
- ES2059401T3 ES2059401T3 ES87400813T ES87400813T ES2059401T3 ES 2059401 T3 ES2059401 T3 ES 2059401T3 ES 87400813 T ES87400813 T ES 87400813T ES 87400813 T ES87400813 T ES 87400813T ES 2059401 T3 ES2059401 T3 ES 2059401T3
- Authority
- ES
- Spain
- Prior art keywords
- protein
- bacterial strain
- cultivation
- obtaining
- mutant bacterial
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormones [GH] (Somatotropin)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
Abstract
ESTE PROCEDIMIENTO CONSISTE EN EMPLEAR UNA CEPA BACTERIANA QUE LLEVA UNA MUTACION LIMITANTE Y HASTA SUPRESORA DE LA EXPRESION DE LOS OPERONES, CUYA TRANSCRIPCION DEPENDE DE LA FIJACION DEL COMPLEJO AMPC-CRP, EN LA QUE HA SIDO INTRODUCIDA UNA SECUENCIA DE ADN CODIFICANTE PARA UN PRECURSOR DE UNA PROTEINA. APLICACION: PRODUCCION DE HGH.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR8605133A FR2597114B1 (fr) | 1986-04-10 | 1986-04-10 | Procede microbiologique pour l'obtention d'une proteine par culture d'une souche bacterienne mutante |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2059401T3 true ES2059401T3 (es) | 1994-11-16 |
Family
ID=9334099
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES87400813T Expired - Lifetime ES2059401T3 (es) | 1986-04-10 | 1987-04-10 | Procedimiento microbiologico para la obtencion de una proteina mediante cultivo de una cepa bacteriana mutante. |
Country Status (7)
Country | Link |
---|---|
US (1) | US4945047A (es) |
EP (1) | EP0245138B1 (es) |
JP (4) | JP2528872B2 (es) |
CA (1) | CA1304021C (es) |
DE (1) | DE3786947T2 (es) |
ES (1) | ES2059401T3 (es) |
FR (1) | FR2597114B1 (es) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5284768A (en) * | 1988-08-24 | 1994-02-08 | Sanofi | Signal peptide, DNA sequences coding for the latter, expression vectors carrying one of these sequences, gram-negative bacteria transformed by these vectors, and process for the periplasmic production of a polypeptide |
FR2636644B1 (fr) * | 1988-08-24 | 1990-12-28 | Sanofi Sa | Sequence d'adn participant a la regulation de l'expression d'une sequence d'adn codant pour un precurseur d'un polypeptide, vecteurs d'expression et procede de production periplasmique du polypeptide |
US5279947A (en) * | 1988-08-24 | 1994-01-18 | Sanofi | DNA sequence participating in the regulation of the expression of a DNA sequence coding for a precursor of a polypeptide, expression vectors and process for the periplasmic production of the polypeptide |
AR088699A1 (es) | 2011-11-09 | 2014-06-25 | Sanofi Sa | Diacilglicerol lipasa y usos de la misma |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS59125895A (ja) * | 1983-01-07 | 1984-07-20 | Rikagaku Kenkyusho | ヒト生長ホルモン遺伝子の合成法 |
US4755465A (en) * | 1983-04-25 | 1988-07-05 | Genentech, Inc. | Secretion of correctly processed human growth hormone in E. coli and Pseudomonas |
JPS60221091A (ja) * | 1983-12-21 | 1985-11-05 | Kyowa Hakko Kogyo Co Ltd | 新規プロモ−タ− |
JPS60234584A (ja) * | 1984-05-09 | 1985-11-21 | Morio Ikehara | 組換プラスミド |
-
1986
- 1986-04-10 FR FR8605133A patent/FR2597114B1/fr not_active Expired - Lifetime
-
1987
- 1987-04-09 US US07/036,263 patent/US4945047A/en not_active Expired - Lifetime
- 1987-04-10 EP EP87400813A patent/EP0245138B1/fr not_active Expired - Lifetime
- 1987-04-10 JP JP62088603A patent/JP2528872B2/ja not_active Expired - Lifetime
- 1987-04-10 CA CA000534359A patent/CA1304021C/en not_active Expired - Lifetime
- 1987-04-10 DE DE87400813T patent/DE3786947T2/de not_active Expired - Lifetime
- 1987-04-10 ES ES87400813T patent/ES2059401T3/es not_active Expired - Lifetime
-
1996
- 1996-02-07 JP JP8021278A patent/JP2661894B2/ja not_active Expired - Lifetime
-
1997
- 1997-02-18 JP JP9034088A patent/JPH09224685A/ja active Pending
- 1997-02-18 JP JP9034087A patent/JP2708403B2/ja not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
CA1304021C (en) | 1992-06-23 |
JPH08228789A (ja) | 1996-09-10 |
JPH09224685A (ja) | 1997-09-02 |
FR2597114A1 (fr) | 1987-10-16 |
JPH09224684A (ja) | 1997-09-02 |
JP2661894B2 (ja) | 1997-10-08 |
JP2528872B2 (ja) | 1996-08-28 |
US4945047A (en) | 1990-07-31 |
JPS637793A (ja) | 1988-01-13 |
FR2597114B1 (fr) | 1994-02-25 |
DE3786947D1 (de) | 1993-09-16 |
EP0245138A1 (fr) | 1987-11-11 |
EP0245138B1 (fr) | 1993-08-11 |
JP2708403B2 (ja) | 1998-02-04 |
DE3786947T2 (de) | 1994-02-24 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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