ES2031826T3 - Procedimiento para producir nuevos peptidos activadores del plasminogeno. - Google Patents

Procedimiento para producir nuevos peptidos activadores del plasminogeno.

Info

Publication number
ES2031826T3
ES2031826T3 ES198686310009T ES86310009T ES2031826T3 ES 2031826 T3 ES2031826 T3 ES 2031826T3 ES 198686310009 T ES198686310009 T ES 198686310009T ES 86310009 T ES86310009 T ES 86310009T ES 2031826 T3 ES2031826 T3 ES 2031826T3
Authority
ES
Spain
Prior art keywords
plasminogen
procedure
produce new
new peptide
activator
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES198686310009T
Other languages
English (en)
Inventor
Nancy L. Haigwood
Guy Mullenbach
Ernest-Gunter Afting
Eric-Paul Paques
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens Healthcare Diagnostics GmbH Germany
Novartis Vaccines and Diagnostics Inc
Original Assignee
Behringwerke AG
Chiron Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Behringwerke AG, Chiron Corp filed Critical Behringwerke AG
Application granted granted Critical
Publication of ES2031826T3 publication Critical patent/ES2031826T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Diabetes (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

SE PRESENTAN NUEVAS COMPOSICIONES DE POLIPEPTIDOS BASADAS EN LA SERIE DE AMINOACIDOS DEL ACTIVADOR DE TEJIDO DE PLASMINOGENO (TPA), QUE TIENEN MEJORES PROPIEDADES QUE EL ACTIVADOR NATURAL DE TEJIDO DE PLASMINOGENO. EN ESPECIAL LA ACTIVIDAD ESPECIFICA AUMENTADA, LA RESPUESTA REDUCIDA A LA INHIBICION POR EL INHIBIDOR DEL ACTIVADOR DE PLASMINOGENO, LA ESTIMULACION DE FIBRINA DE LA ACTIVIDAD PLASMINOGENOLITICA Y/O LA AFINIDAD MEJORADA PARA LAS SUPERFICIES DE FIBRINA, SE CONSIGUEN POR MODIFICACION DE UNO O MAS LOCI POR SUPRESIONES O SUSTITUCIONES. PUEDEN MODIFICARSE UNO O AMBOS TERMINALES N-OC-.
ES198686310009T 1985-12-23 1986-12-22 Procedimiento para producir nuevos peptidos activadores del plasminogeno. Expired - Lifetime ES2031826T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US81287985A 1985-12-23 1985-12-23

Publications (1)

Publication Number Publication Date
ES2031826T3 true ES2031826T3 (es) 1993-01-01

Family

ID=25210860

Family Applications (1)

Application Number Title Priority Date Filing Date
ES198686310009T Expired - Lifetime ES2031826T3 (es) 1985-12-23 1986-12-22 Procedimiento para producir nuevos peptidos activadores del plasminogeno.

Country Status (10)

Country Link
US (4) US5501853A (es)
EP (1) EP0227462B1 (es)
JP (4) JP2575369B2 (es)
AT (1) ATE74379T1 (es)
AU (1) AU605124B2 (es)
DE (1) DE3684680D1 (es)
DK (1) DK623186A (es)
ES (1) ES2031826T3 (es)
GR (1) GR3004568T3 (es)
ZA (1) ZA869626B (es)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO175158C (no) * 1984-10-01 1994-09-07 Genzyme Corp Fremstilling av human livmorsvevplasminogenaktivator med rekombinant-DNA
DE3684680D1 (de) * 1985-12-23 1992-05-07 Chiron Corp Peptidplasminogenaktivatoren.
US5837518A (en) * 1986-01-31 1998-11-17 Genetics Institute, Inc. Thrombolytic proteins
WO1987004722A1 (en) * 1986-01-31 1987-08-13 Genetics Institute, Inc. Novel thrombolytic proteins
US5002887A (en) * 1986-01-31 1991-03-26 Genetics Institute, Inc. Truncated thrombolytic proteins
US5258298A (en) * 1986-01-31 1993-11-02 Genetics Institute, Inc. Deletion and glycosylation mutant of human tissue plasminogen activator
ATE70301T1 (de) * 1986-02-17 1991-12-15 Stichting Centraal Lab Gewebeplasminogen-aktivator-mutant, dafuer kodierende rekombinante genetische information und verfahren zur herstellung dieser mutanten, deren verwendung und pharmazeutische zusammensetzungen.
IE81073B1 (en) * 1986-03-18 2000-01-12 Genentech Inc Modified human tissue-type plasminogen activator and its preparation
US5047241A (en) * 1986-07-11 1991-09-10 American Home Products Corporation Tris-Kringle plasminogen activator with novel K2 insert
US5580559A (en) * 1986-12-05 1996-12-03 Ciba-Geigy Corporation Hybrid plasminogen activator
US5242819A (en) * 1986-12-05 1993-09-07 Ciba-Geigy Corporation DNA molecules encoding hybrid proteins of tissue plasminogen activator and urokinase
DK275788A (da) * 1987-05-22 1989-02-03 Zymogenetics Inc Fibrinolytiske proteiner
US5149533A (en) * 1987-06-04 1992-09-22 Zymogenetics, Inc. Modified t-PA with kringle-/replaced by another kringle
SE8702562L (sv) * 1987-06-18 1988-12-19 Kabigen Ab Nya fibrinolytiska enzymer
US5302390A (en) * 1987-07-01 1994-04-12 Beecham Group Plc Hybrid proteins of human plasminogen and human t-PA, pharmaceutical compositions and methods of treatment
JP2708749B2 (ja) * 1987-08-10 1998-02-04 エーザイ株式会社 修飾型ｔＰＡ含有注射用組成物
ATE103332T1 (de) * 1987-10-09 1994-04-15 Monsanto Co Modifizierter gewebeplasminogenaktivator.
US5037752A (en) * 1987-10-09 1991-08-06 Monsanto Company Modified tissue plasminogen activator substituted at cysteine-73 and lysine-277
US5244676A (en) * 1987-10-09 1993-09-14 Monsanto Company Modified tissue plasminogen activator with modified glycosylation site
US4963357A (en) * 1987-10-09 1990-10-16 Monsanto Company Tissue plasminogen activator modified by the substitution of Arg for Cys at position 73, methods and pharmaceutical compositions therefor
AU618039B2 (en) * 1987-10-29 1991-12-12 Yamanouchi Pharmaceutical Co., Ltd. Novel polypeptide compounds
US5094953A (en) * 1988-03-21 1992-03-10 Genentech, Inc. Human tissue plasminogen activator variants
US5346824A (en) * 1988-05-20 1994-09-13 Genentech, Inc. DNA encoding variants of tissue plasminogen activators and expression vectors and hosts thereof
IL90146A (en) * 1988-05-20 1994-10-07 Genentech Inc Glycosylation derivatives of tissue plasminogen activator
US5270198A (en) * 1988-05-20 1993-12-14 Genentech, Inc. DNA molecules encoding variants of tissue plasminogen activators, vectors, and host cells
DE3823805A1 (de) * 1988-07-14 1990-01-18 Basf Ag Neue polypeptide, ihre herstellung und verwendung
US5258180A (en) * 1988-09-02 1993-11-02 Genetech, Inc. Tissue plasminogen activator having fibrin specific properties and deletion of amino acids 466-970, compositions and methods of treatment
US5262170A (en) * 1988-09-02 1993-11-16 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties and substituted at amino acid positions 296-299, DNA molecules encoding them, vectors, and host cells
US5714145A (en) * 1988-09-02 1998-02-03 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties
US5108901A (en) * 1988-09-02 1992-04-28 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties
US5070021A (en) * 1989-01-10 1991-12-03 Monsanto Company Method of modifying oligosaccharide structure of tissue plasminogen activator
CA2007364A1 (en) * 1989-01-17 1990-07-17 David J. Livingston Gene sequences encoding modified residues situated in the protease domain of tpa
IT1243774B (it) * 1990-08-02 1994-06-28 Erba Carlo Spa Derivati della prourochinasi
US5223408A (en) * 1991-07-11 1993-06-29 Genentech, Inc. Method for making variant secreted proteins with altered properties
DK0643772T3 (da) * 1992-06-03 1998-02-02 Genentech Inc Glycosyleringsvarianter af vævsplasminogenaktivator med forbedrede terapeutiske egenskaber
ATE348173T1 (de) 1999-04-26 2007-01-15 Genentech Inc Zellenzuchtverfahren für glycoproteine
US6649597B1 (en) 2000-06-22 2003-11-18 The University Of Iowa Research Foundation Targeting vector to the urokinase plasminogen activator receptor
WO2002020802A1 (fr) * 2000-09-04 2002-03-14 Hunan Royal Biotech Lignee cellulaire exprimant un activateur de plasminogene tissulaire humain mutant, procede de recombinaison et procede de preparation de la proteine exprimee
IL145209A0 (en) * 2000-09-06 2002-06-30 Pfizer Prod Inc Pharmaceutical combinations for the treatment of stroke and traumatic brain injury
KR100701265B1 (ko) * 2006-01-16 2007-04-02 대한민국 재조합 사람 조직형 플라스미노겐 활성인자 발현벡터 및 플라스미노겐 활성인자를 소변으로 분비하는 형질전환돼지 생산방법
KR100834561B1 (ko) * 2006-12-08 2008-06-02 가톨릭대학교 산학협력단 크링글 도메인 1 및 2로 이루어진 비-글리코실화 재조합단백질, 그 제조 방법 및 이를 포함하는 조성물
US8314212B2 (en) 2008-02-08 2012-11-20 Lindsey Miles Plasminogen receptor PLG-RKT and antibodies thereof
KR100855024B1 (ko) 2008-04-30 2008-08-28 가톨릭대학교 산학협력단 크링글 도메인 １ 및 ２ 로 이루어진 비-글리코실화 재조합단백질

Family Cites Families (29)

* Cited by examiner, † Cited by third party
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SU1662352A3 (ru) * 1982-05-05 1991-07-07 Генентек, Инк (Фирма) Штамм бактерий ЕSснеRIснIа coLI - продуцент активатора плазминогена тканевого типа
IL68561A (en) * 1982-05-05 1991-01-31 Genentech Inc Preparation of polypeptide with human tissue plasminogen activator function,processes for making it,and dna and transformed cell intermediates thereof
EP0125254A1 (en) * 1982-10-28 1984-11-21 Beecham Group Plc Enzyme derivatives and their use in the treatment of thrombosis
GB8401786D0 (en) 1984-01-24 1984-02-29 Ray M S Design and operation of adsorbent container
US4753879A (en) * 1984-08-27 1988-06-28 Biogen N.V. Modified tissue plasminogen activators
NO175158C (no) * 1984-10-01 1994-09-07 Genzyme Corp Fremstilling av human livmorsvevplasminogenaktivator med rekombinant-DNA
JPS61247384A (ja) * 1984-10-18 1986-11-04 チモ−ジエネテイツクス　インコ−ポレ−テツド 酵母中でプラスミノ−ゲン活性化因子を発現する方法
DE3537176C2 (de) * 1984-10-18 1994-06-09 Zymogenetics Inc Gewebeplasminogenaktivator und Verfahren zu seiner Herstellung
EP0201153A3 (en) * 1985-02-09 1987-10-07 Beecham Group Plc Modified enzyme and process for its preparation
GB8503358D0 (en) * 1985-02-09 1985-03-13 Beecham Group Plc Compounds
GR861037B (en) * 1985-04-22 1986-07-16 Genentech Inc Novel human tissue plasminogen activator mutants
US5073494A (en) * 1985-04-22 1991-12-17 Genentech, Inc. Human tissue plasminogen activator substituted at position 275 or at positions 275 and 277
JPH0811066B2 (ja) * 1985-09-30 1996-02-07 インテグレーテッド・ジエネティックス・インコーポレーテッド 組換えｄｎａが生産するヒト子宮プラスミノ−ゲンアクチベ−タ−
GB8528321D0 (en) * 1985-11-18 1985-12-24 Ciba Geigy Ag Modified fibrinolytic agents
US5106741A (en) * 1985-12-20 1992-04-21 The Upjohn Company Tissue plasminogen activator (TPA) analogs
ES2053449T3 (es) * 1985-12-20 1994-08-01 Upjohn Co Analogos (tpa) de activadores de plasminogeno de tejido.
DE3684680D1 (de) * 1985-12-23 1992-05-07 Chiron Corp Peptidplasminogenaktivatoren.
ATE70301T1 (de) * 1986-02-17 1991-12-15 Stichting Centraal Lab Gewebeplasminogen-aktivator-mutant, dafuer kodierende rekombinante genetische information und verfahren zur herstellung dieser mutanten, deren verwendung und pharmazeutische zusammensetzungen.
US4751084A (en) * 1986-02-26 1988-06-14 Monsanto Company Tissue plasminogen activator from normal human colon cells
IE81073B1 (en) * 1986-03-18 2000-01-12 Genentech Inc Modified human tissue-type plasminogen activator and its preparation
WO1987005934A1 (en) * 1986-03-28 1987-10-08 Roberto Crea Protein analogues of tissue plasminogen activator
JPH0761266B2 (ja) * 1986-07-03 1995-07-05 株式会社ミドリ十字 変異ヒトプロウロキナ−ゼ
DK345087A (da) * 1986-07-11 1988-01-12 Novo Industri As Modificeret vaevsplasminogenaktivator
EP0266032A1 (en) * 1986-08-29 1988-05-04 Beecham Group Plc Modified fibrinolytic enzyme
PT86162B (pt) * 1986-11-21 1990-11-20 Smithkline Beecham Corp Expressao de proteina recombinante
NL8700013A (nl) * 1987-01-06 1988-08-01 Leuven Res & Dev Vzw Hybride plasminogeenactivatoren met verbeterde trombolytische eigenschappen en geneesmiddelen die deze plasminogeenactivatoren bevatten.
GB8705377D0 (en) * 1987-03-07 1987-04-08 Beecham Group Plc Compounds
US5009888A (en) * 1987-04-13 1991-04-23 Genzyme Corporation Therapeutic enzyme-antibody complexes
US5258180A (en) * 1988-09-02 1993-11-02 Genetech, Inc. Tissue plasminogen activator having fibrin specific properties and deletion of amino acids 466-970, compositions and methods of treatment

Also Published As

Publication number Publication date
JPH0787973A (ja) 1995-04-04
DE3684680D1 (de) 1992-05-07
EP0227462B1 (en) 1992-04-01
GR3004568T3 (es) 1993-04-28
JPH09187287A (ja) 1997-07-22
US5656269A (en) 1997-08-12
JP2575369B2 (ja) 1997-01-22
US5501853A (en) 1996-03-26
JPS62253380A (ja) 1987-11-05
JP2681000B2 (ja) 1997-11-19
ZA869626B (en) 1987-10-28
US5976530A (en) 1999-11-02
JPH0775583A (ja) 1995-03-20
AU605124B2 (en) 1991-01-10
JP2680999B2 (ja) 1997-11-19
AU6683086A (en) 1987-06-25
JP2769314B2 (ja) 1998-06-25
US5439679A (en) 1995-08-08
DK623186D0 (da) 1986-12-22
EP0227462A2 (en) 1987-07-01
ATE74379T1 (de) 1992-04-15
EP0227462A3 (en) 1987-10-07
DK623186A (da) 1987-08-26

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