EP2048228B8 - Human liver progenitors - Google Patents

Human liver progenitors Download PDF

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Publication number
EP2048228B8
EP2048228B8 EP08019659.5A EP08019659A EP2048228B8 EP 2048228 B8 EP2048228 B8 EP 2048228B8 EP 08019659 A EP08019659 A EP 08019659A EP 2048228 B8 EP2048228 B8 EP 2048228B8
Authority
EP
European Patent Office
Prior art keywords
progenitors
suspension
human liver
cell
cell lineages
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Revoked
Application number
EP08019659.5A
Other languages
German (de)
English (en)
French (fr)
Other versions
EP2048228A2 (en
EP2048228A3 (en
EP2048228B1 (en
Inventor
Lola M. Reid
Hiroshi Kubota
Nicholas Moss
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of North Carolina at Chapel Hill
Original Assignee
University of North Carolina at Chapel Hill
University of North Carolina System
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22366551&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EP2048228(B8) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by University of North Carolina at Chapel Hill, University of North Carolina System filed Critical University of North Carolina at Chapel Hill
Publication of EP2048228A2 publication Critical patent/EP2048228A2/en
Publication of EP2048228A3 publication Critical patent/EP2048228A3/en
Application granted granted Critical
Publication of EP2048228B1 publication Critical patent/EP2048228B1/en
Publication of EP2048228B8 publication Critical patent/EP2048228B8/en
Anticipated expiration legal-status Critical
Revoked legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0672—Stem cells; Progenitor cells; Precursor cells; Oval cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
    • A61K2039/55588—Adjuvants of undefined constitution
    • A61K2039/55594—Adjuvants of undefined constitution from bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/22—Zinc; Zn chelators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/02—Drug screening

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Hematology (AREA)
  • Diabetes (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP08019659.5A 1999-01-19 2000-01-19 Human liver progenitors Revoked EP2048228B8 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11633199P 1999-01-19 1999-01-19
EP00906940A EP1147179B1 (en) 1999-01-19 2000-01-19 Human liver progenitors

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
EP00906940A Division EP1147179B1 (en) 1999-01-19 2000-01-19 Human liver progenitors

Publications (4)

Publication Number Publication Date
EP2048228A2 EP2048228A2 (en) 2009-04-15
EP2048228A3 EP2048228A3 (en) 2009-04-22
EP2048228B1 EP2048228B1 (en) 2016-03-23
EP2048228B8 true EP2048228B8 (en) 2016-05-11

Family

ID=22366551

Family Applications (2)

Application Number Title Priority Date Filing Date
EP00906940A Expired - Lifetime EP1147179B1 (en) 1999-01-19 2000-01-19 Human liver progenitors
EP08019659.5A Revoked EP2048228B8 (en) 1999-01-19 2000-01-19 Human liver progenitors

Family Applications Before (1)

Application Number Title Priority Date Filing Date
EP00906940A Expired - Lifetime EP1147179B1 (en) 1999-01-19 2000-01-19 Human liver progenitors

Country Status (19)

Country Link
US (3) US20020182188A1 (zh)
EP (2) EP1147179B1 (zh)
JP (1) JP5068901B2 (zh)
KR (1) KR100873690B1 (zh)
CN (2) CN102703379B (zh)
AT (1) ATE414143T1 (zh)
AU (1) AU779706B2 (zh)
CA (2) CA2360730C (zh)
CY (1) CY1108806T1 (zh)
DE (1) DE60040782D1 (zh)
DK (2) DK1147179T3 (zh)
ES (2) ES2569778T3 (zh)
HK (2) HK1046930B (zh)
IL (2) IL144411A0 (zh)
NZ (3) NZ513150A (zh)
PT (1) PT1147179E (zh)
RU (1) RU2247565C2 (zh)
WO (1) WO2000043498A2 (zh)
ZA (1) ZA200106306B (zh)

Families Citing this family (52)

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FR2806911B1 (fr) * 2000-03-28 2003-01-10 Univ Rene Descartes Utilisation de mimetiques de la sod dans le traitement d'insuffisances hepatocellulaires
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US6458589B1 (en) 2000-04-27 2002-10-01 Geron Corporation Hepatocyte lineage cells derived from pluripotent stem cells
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CA2424779C (en) * 2000-10-03 2016-08-23 University Of North Carolina At Chapel Hill Processes for clonal growth of hepatic progenitor cells
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JP2002534974A (ja) 2002-10-22
EP2048228A2 (en) 2009-04-15
CN102703379B (zh) 2015-06-03
CN1351656A (zh) 2002-05-29
WO2000043498A2 (en) 2000-07-27
ES2569778T3 (es) 2016-05-12
CY1108806T1 (el) 2014-04-09
US20020182188A1 (en) 2002-12-05
CN102703379A (zh) 2012-10-03
ZA200106306B (en) 2002-07-31
ES2316353T3 (es) 2009-04-16
AU779706B2 (en) 2005-02-10
HK1046930B (zh) 2012-08-10
KR20020013492A (ko) 2002-02-20
US20100197015A1 (en) 2010-08-05
HK1046930A1 (en) 2003-01-30
CA2877233A1 (en) 2000-07-27
NZ513150A (en) 2004-09-24
KR100873690B1 (ko) 2008-12-12
PT1147179E (pt) 2009-02-12
NZ537749A (en) 2007-02-23
NZ531720A (en) 2005-07-29
CA2360730C (en) 2015-04-14
DE60040782D1 (de) 2008-12-24
JP5068901B2 (ja) 2012-11-07
DK1147179T3 (da) 2009-03-09
CA2360730A1 (en) 2000-07-27
US20050148072A1 (en) 2005-07-07
IL144411A (en) 2007-06-03
ATE414143T1 (de) 2008-11-15
AU2852000A (en) 2000-08-07
RU2247565C2 (ru) 2005-03-10
WO2000043498A9 (en) 2001-10-18
WO2000043498A3 (en) 2000-12-14
EP2048228A3 (en) 2009-04-22
EP2048228B1 (en) 2016-03-23
HK1176641A1 (zh) 2013-08-02
CN1351656B (zh) 2012-02-29
EP1147179A2 (en) 2001-10-24
IL144411A0 (en) 2002-05-23
DK2048228T3 (en) 2016-05-09

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