EP1877773B1 - Neue apparatur und verfahren zur beschichtung von trägersubstraten für einen analytnachweis mittels affinitäts-nachweisverfahren - Google Patents

Neue apparatur und verfahren zur beschichtung von trägersubstraten für einen analytnachweis mittels affinitäts-nachweisverfahren Download PDF

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Publication number
EP1877773B1
EP1877773B1 EP06724515.9A EP06724515A EP1877773B1 EP 1877773 B1 EP1877773 B1 EP 1877773B1 EP 06724515 A EP06724515 A EP 06724515A EP 1877773 B1 EP1877773 B1 EP 1877773B1
Authority
EP
European Patent Office
Prior art keywords
liquid
substrates
atomized
coating
carrier substrates
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Not-in-force
Application number
EP06724515.9A
Other languages
German (de)
English (en)
French (fr)
Other versions
EP1877773A1 (de
Inventor
Eginhard Schick
Dominic Utinger
Claudio Calonder
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer Intellectual Property GmbH
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Bayer Intellectual Property GmbH
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Filing date
Publication date
Application filed by Bayer Intellectual Property GmbH filed Critical Bayer Intellectual Property GmbH
Publication of EP1877773A1 publication Critical patent/EP1877773A1/de
Application granted granted Critical
Publication of EP1877773B1 publication Critical patent/EP1877773B1/de
Not-in-force legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
    • B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
    • B05B17/00—Apparatus for spraying or atomising liquids or other fluent materials, not covered by the preceding groups
    • B05B17/04—Apparatus for spraying or atomising liquids or other fluent materials, not covered by the preceding groups operating with special methods
    • B05B17/06—Apparatus for spraying or atomising liquids or other fluent materials, not covered by the preceding groups operating with special methods using ultrasonic or other kinds of vibrations
    • B05B17/0607—Apparatus for spraying or atomising liquids or other fluent materials, not covered by the preceding groups operating with special methods using ultrasonic or other kinds of vibrations generated by electrical means, e.g. piezoelectric transducers
    • B05B17/0615—Apparatus for spraying or atomising liquids or other fluent materials, not covered by the preceding groups operating with special methods using ultrasonic or other kinds of vibrations generated by electrical means, e.g. piezoelectric transducers spray being produced at the free surface of the liquid or other fluent material in a container and subjected to the vibrations
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers

Definitions

  • the inventive method is also characterized by the possibility of simultaneous coating of a large number of carrier substrates in a common, appropriately sized container and ease of automation and is easily feasible even for untrained personnel.
  • the liquid volumes to be used for coating the carrier substrates are of similar order of magnitude as in the case of the spray method.
  • a coating system according to the invention to produce geometrically structured coatings in a coating method according to the invention by optionally sequential atomization of one or more optionally different liquids.
  • the prerequisite for producing reproducible coated regions on the carrier substrates in their geometry is that areas of the carrier substrate that are not to be coated in each case are covered fluidically by a corresponding suitable mask, so that no mist droplets reach the areas not to be coated.
  • the coating apparatus additionally comprises provisions for controlling and / or regulating the pressure within the liquid container during the coating process.
  • luminescence in this application refers to the spontaneous emission of photons in the ultraviolet to infrared range after optical or non-optical, such as electrical or chemical or biochemical or thermal excitation.
  • chemiluminescence, bioluminescence, electroluminescence and in particular fluorescence and phosphorescence are included under the term “luminescence”. Fluorescence and phosphorescence are particularly preferred forms of luminescence.
  • the discrete or continuous waveguiding regions of the carrier substrates to be coated are in contact with one or more grating structures (c), which enable the coupling of excitation light or measuring light into waveguiding layers of said carrier substrates, and / or to one or more grating structures (c '), which enable the decoupling of excitation light or measuring light from waveguiding layers of said carrier substrates are, wherein in the case of simultaneously present on a carrier substrate grating structures (c) and (c') may have the same or different grating periods.
  • a mist eliminator to be used may also be permeable to drops up to a defined size. This can be technically realized, for example, by virtue of the fact that the mist eliminator comprises a fine-meshed net, with the mesh spacing of which the maximum size of drops to be passed is determined.
  • the coating apparatus additionally comprises at least one gas inlet via which a gas is introduced into the liquid container, which gas mixes with the generated mist.
  • the apparatus may additionally include one or more outlets for venting gas and / or mist.
  • the liquid container of the coating apparatus is closed except for optional inlets for gas and optional additional outlets for gas and / or mist.
  • FIG Fig. 1 A schematic representation of a coating apparatus according to the invention is shown in FIG Fig. 1 shown.
  • the areas of unprotected areas of specific binding partners are "passivated” by carrier substrates prepared for an affinity detection method, ie a "passivation layer” is applied in these areas.
  • the background signals are determined from the measured signal intensities between the spots.
  • four further circular areas are defined as evaluation areas for background signal determination per spot, which are preferably arranged in the middle between between adjacent spots.
  • the average background signal intensity is determined, for example, as the arithmetic mean of the pixel values (signal intensities) within an AOI selected for this purpose.
  • the average net signal intensity from the measurement areas (spots) is then calculated as the difference between the local average gross and local mean background signal intensity of the respective spot.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Application Of Or Painting With Fluid Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
EP06724515.9A 2005-04-26 2006-04-22 Neue apparatur und verfahren zur beschichtung von trägersubstraten für einen analytnachweis mittels affinitäts-nachweisverfahren Not-in-force EP1877773B1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CH7352005 2005-04-26
PCT/EP2006/003726 WO2006114249A1 (de) 2005-04-26 2006-04-22 Neue apparatur und verfahren zur beschichtung von trägersubstraten für einen analytnachweis mittels affinitäts-nachweisverfahren

Publications (2)

Publication Number Publication Date
EP1877773A1 EP1877773A1 (de) 2008-01-16
EP1877773B1 true EP1877773B1 (de) 2014-10-15

Family

ID=36808724

Family Applications (1)

Application Number Title Priority Date Filing Date
EP06724515.9A Not-in-force EP1877773B1 (de) 2005-04-26 2006-04-22 Neue apparatur und verfahren zur beschichtung von trägersubstraten für einen analytnachweis mittels affinitäts-nachweisverfahren

Country Status (9)

Country Link
US (1) US9050615B2 (enExample)
EP (1) EP1877773B1 (enExample)
JP (1) JP5002584B2 (enExample)
CN (1) CN101208599B (enExample)
AU (1) AU2006239534B2 (enExample)
CA (1) CA2605750A1 (enExample)
DK (1) DK1877773T3 (enExample)
ES (1) ES2525324T3 (enExample)
WO (1) WO2006114249A1 (enExample)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2485564A (en) * 2010-11-18 2012-05-23 Rolls Royce Plc Luminescent surface coating for inspection
WO2013001431A1 (en) 2011-06-30 2013-01-03 Koninklijke Philips Electronics N.V. Multiple examinations of a sample
JP6384491B2 (ja) 2014-01-10 2018-09-05 コニカミノルタ株式会社 イムノアッセイ法およびイムノアッセイシステム
DE102014112625A1 (de) * 2014-09-02 2016-03-03 Schmid Energy Systems Gmbh Großflächen-Ultraschallverdampfer
EP3229977A4 (en) * 2014-12-08 2019-02-27 University of Virginia Patent Foundation, D/B/A University of Virginia COMPOSITIONS AND METHODS FOR BONDING ADHESIVES, FABRICS AND COATINGS ON SURFACES
CH712376A1 (de) 2016-04-19 2017-10-31 Camag Derivatisierungsgerät und -verfahren.
CN106198952A (zh) * 2016-07-01 2016-12-07 清华大学 一种抑制核酸分子对传感界面非特异性吸附的封闭方法
CN107807133A (zh) * 2017-11-08 2018-03-16 苏州安路特汽车部件有限公司 一种雾化渗透探伤装置
EP3963091A4 (en) * 2019-04-29 2023-07-19 Nautilus Biotechnology, Inc. METHODS AND SYSTEMS FOR ON-CHIP INTEGRATED SINGLE MOLECULE DETECTION
CN115147617B (zh) * 2022-09-06 2022-11-22 聊城集众环保科技有限公司 基于计算机视觉的污水处理智能监控方法
CN115837944A (zh) * 2023-02-16 2023-03-24 太原理工大学 一种电厂烟气注入采空区防灾封存的气体监测装置

Citations (1)

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JPH03296460A (ja) * 1990-04-13 1991-12-27 Fujitsu General Ltd フラックス塗付装置
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Also Published As

Publication number Publication date
AU2006239534A1 (en) 2006-11-02
JP5002584B2 (ja) 2012-08-15
US9050615B2 (en) 2015-06-09
AU2006239534B2 (en) 2012-05-31
US20090311773A1 (en) 2009-12-17
CN101208599B (zh) 2014-12-10
WO2006114249A1 (de) 2006-11-02
CN101208599A (zh) 2008-06-25
DK1877773T3 (en) 2015-01-19
EP1877773A1 (de) 2008-01-16
JP2008539399A (ja) 2008-11-13
ES2525324T3 (es) 2014-12-22
CA2605750A1 (en) 2006-11-02

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