EP1686961A1 - Formes solides d'anticorps anti-egfr - Google Patents

Formes solides d'anticorps anti-egfr

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Publication number
EP1686961A1
EP1686961A1 EP04797849A EP04797849A EP1686961A1 EP 1686961 A1 EP1686961 A1 EP 1686961A1 EP 04797849 A EP04797849 A EP 04797849A EP 04797849 A EP04797849 A EP 04797849A EP 1686961 A1 EP1686961 A1 EP 1686961A1
Authority
EP
European Patent Office
Prior art keywords
antibody
protein
egfr
antibodies
solid forms
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
EP04797849A
Other languages
German (de)
English (en)
Inventor
Susanne Matheus
Hanns-Christian Mahler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Patent GmbH
Original Assignee
Merck Patent GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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Application filed by Merck Patent GmbH filed Critical Merck Patent GmbH
Priority to EP08011237A priority Critical patent/EP1974723A3/fr
Publication of EP1686961A1 publication Critical patent/EP1686961A1/fr
Ceased legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2863—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A61K39/39591—Stabilisation, fragmentation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C30—CRYSTAL GROWTH
    • C30B—SINGLE-CRYSTAL GROWTH; UNIDIRECTIONAL SOLIDIFICATION OF EUTECTIC MATERIAL OR UNIDIRECTIONAL DEMIXING OF EUTECTOID MATERIAL; REFINING BY ZONE-MELTING OF MATERIAL; PRODUCTION OF A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; SINGLE CRYSTALS OR HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; AFTER-TREATMENT OF SINGLE CRYSTALS OR A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; APPARATUS THEREFOR
    • C30B7/00—Single-crystal growth from solutions using solvents which are liquid at normal temperature, e.g. aqueous solutions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/141—Intimate drug-carrier mixtures characterised by the carrier, e.g. ordered mixtures, adsorbates, solid solutions, eutectica, co-dried, co-solubilised, co-kneaded, co-milled, co-ground products, co-precipitates, co-evaporates, co-extrudates, co-melts; Drug nanoparticles with adsorbed surface modifiers
    • A61K9/143—Intimate drug-carrier mixtures characterised by the carrier, e.g. ordered mixtures, adsorbates, solid solutions, eutectica, co-dried, co-solubilised, co-kneaded, co-milled, co-ground products, co-precipitates, co-evaporates, co-extrudates, co-melts; Drug nanoparticles with adsorbed surface modifiers with inorganic compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/141—Intimate drug-carrier mixtures characterised by the carrier, e.g. ordered mixtures, adsorbates, solid solutions, eutectica, co-dried, co-solubilised, co-kneaded, co-milled, co-ground products, co-precipitates, co-evaporates, co-extrudates, co-melts; Drug nanoparticles with adsorbed surface modifiers
    • A61K9/145—Intimate drug-carrier mixtures characterised by the carrier, e.g. ordered mixtures, adsorbates, solid solutions, eutectica, co-dried, co-solubilised, co-kneaded, co-milled, co-ground products, co-precipitates, co-evaporates, co-extrudates, co-melts; Drug nanoparticles with adsorbed surface modifiers with organic compounds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2299/00—Coordinates from 3D structures of peptides, e.g. proteins or enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered

Definitions

  • the invention relates to solid forms of antibodies to the EGF receptor (EGFR), in particular precipitates and crystals of monoclonal antibodies to the EGF receptor, more preferably Mab C225 (cetuximab) and Mab h425 (EMD 72000), which are characterized by Dissolving or suspending in aqueous or nonaqueous medium to biologically active antibody protein, obtainable by precipitation, the antibody dissolved or suspended in an aqueous medium and / or one of its variants and / or fragments by means of a precipitation reagent.
  • EGFR EGF receptor
  • the invention further relates to pharmaceutical preparations containing at least one solid form of the abovementioned antibodies in precipitated non-crystalline, precipitated crystalline or in dissolved or suspended form, and optionally excipients and / or adjuvants and / or other active pharmaceutical ingredients, as well as a process for the preparation according to the invention solid forms of anti-EGFR antibodies.
  • pharmaceutical preparations containing at least one solid form of the abovementioned antibodies in precipitated non-crystalline, precipitated crystalline or in dissolved or suspended form, and optionally excipients and / or adjuvants and / or other active pharmaceutical ingredients, as well as a process for the preparation according to the invention solid forms of anti-EGFR antibodies.
  • Protein drugs such as monoclonal antibodies are used, for example, in tumor therapy, for example for specific immunotherapy or tumor vaccination.
  • Therapeutic proteins are larger and more complex than conventional organic and inorganic drugs, and have complex three-dimensional structures and numerous functional groups that can cause the biological activity of the protein or even cause undesirable effects. Protein drugs are involved in numerous exogenous effects during production, storage, and transport exposed, which reduces stability can affect the protein drug. It is therefore necessary to study precisely the causes and mechanisms of the specific degradation reactions in order to be able to stabilize the protein, for example by adding certain stabilizing auxiliaries (see eg Manning MC, Patel K., & Borchardt RT (1989) Stability of protein pharmaceuticals Res. 6,
  • Chemical instabilities are characterized by covalent modifications of the protein.
  • the primary structure of the protein changes due to breakage, rebuilding or reformation of chemical bonds.
  • the newly formed substance usually differs completely in biological activity from the original, native protein.
  • Physical instabilities alter the spatial arrangement of the molecule (the secondary, tertiary, and quaternary structures) without destroying covalent bonds. They can be used in denaturation, association, aggregation,
  • Precipitates are the macroscopically visible equivalent of aggregates and arise mechanistically through clusters of aggregates or associates.
  • the flakes are visually visible from a diameter of about 10 microns through a light microscope and from about 50 microns.
  • the Protein aggregation may be a reversible or irreversible process (see, eg, Cleland JL, Powell MF & Shire SJ (1993) The development of stable protein formulations: A look at protein aggregation, deamidation, and oxidation Grit Rev. Ther. Drug Carrier Syst 10, 307-377).
  • Protein crystals are from the purification process (down stream processing) preferably of enzymes and to elucidate the
  • lyophilization for the stabilization of protein formulations is known, for example, from WO9300807 or WO9822136, but significant disadvantages of lyophilized preparations are that the user still has to reconstitute the lyophilizate before use, which constitutes a significant source of error in the preparation before use , Since there is an additional manufacturing process compared to liquid formulations, the process is additional in terms of process development (ensuring stability in lyophilization), manufacturing (manufacturing cost and time), and e.g. Validation unfavorable.
  • solid forms of the abovementioned antibodies can be prepared and that these solid forms and formulations prepared therefrom do not have the disadvantages mentioned in the prior art.
  • the solid forms of anti-EGFR antibodies according to the invention described below and / or the formulations prepared therefrom are surprisingly distinguished by one or more advantages selected from: high stability, controllable particle size, protein after re-dissolution or native and biologically active resuspension, high Purity, absence of pharmaceutically questionable agents and thus high safety, good tolerability and possibility of d irective use, low aggregation tendency and thus the ability to produce highly concentrated formulations and bi formulation as a protein suspension compared to a low viscosity compared to a solution.
  • the production method according to the invention described below is surprisingly distinguished by one or more advantages selected from: simplicity, time and cost savings, use of pharmaceutically acceptable agents, high yield.
  • the process of the invention is thus preferably much easier, more time-saving and more cost-effective to carry out than the techniques described in the literature, since only the addition of a single precipitation reagent is necessary.
  • Antibodies are used directly in pharmaceuticals, further purification to remove pharmacologically harmful agents is not necessary.
  • the crystals obtained in WO02072636 must be prepared in a complex process of pharmacologically questionable agents, for example CHES, imidazole, tris, manganese (II) chloride,
  • Zinc (II) chloride, copper (II) sulfate, 2-propanol, 2-methoyl-2,4-pentanediol, HEPES, lithium sulfate, ethoxyethanol or detergents such as polysorbate 80 or 20 are cleaned or it is not possible completely remove the above mentioned questionable agents.
  • anti-EGFR antibodies antibodies against the EGF receptor
  • monoclonal anti-EGFR antibodies more preferably Mab C225 (cetuximab) or Mab h425 (EMD 72000)
  • certain precipitation reagents selected from
  • Polymers preferably polyethylene glycol (PEG), salts, preferably ammonium sulfate or organic solvents, preferably ethanol, or mixtures thereof, in the presence of a suitable buffer, at a suitable pH and temperature are incubated.
  • PEG polyethylene glycol
  • salts preferably ammonium sulfate or organic solvents, preferably ethanol, or mixtures thereof
  • the solid forms of anti-EGFR antibodies obtained by the method according to the invention preferably of monoclonal anti-EGFR antibodies, more preferably of Mab C225 (cetuximab) or Mab h425 (EMD 72000) and / or variants or fragments thereof , preferably are native after reconstitution and are preferably obtained in a high yield.
  • the invention therefore relates to solid forms of anti-EGFR antibodies and / or their variants or fragments which, by dissolving or suspending in an aqueous medium, lead to biologically active antibody protein obtainable by precipitation of the antibody dissolved or suspended in an aqueous medium and / or its
  • Variants and / or fragments by means of a precipitation reagent selected from polymers, preferably polyethylene glycol (PEG), salts, preferably ammonium sulfate, or organic solvents, preferably ethanol, or mixtures thereof, in the presence of a suitable buffer, at a suitable pH and temperature.
  • a precipitation reagent selected from polymers, preferably polyethylene glycol (PEG), salts, preferably ammonium sulfate, or organic solvents, preferably ethanol, or mixtures thereof, in the presence of a suitable buffer, at a suitable pH and temperature.
  • Solid forms of an anti-EGFR antibody according to the invention are preferably understood to mean precipitates or crystals which lead to biologically active antibodies by dissolving or suspending in an aqueous or non-aqueous medium.
  • Solid forms of the invention are obtained by precipitating the antibody dissolved or suspended in an aqueous medium according to the methods described below.
  • the solid forms of the invention may be in the range of ⁇ m and nm.
  • precipitates are understood to mean solid forms in an amorphous noncrystalline structure or states of aggregation and association.
  • crystals are understood to mean solid forms in a crystalline structure. Crystalline structures can be detected by the methods below.
  • precipitated means solid forms according to the invention in precipitated form, ie, depending on the process conditions, in the form of a precipitate or a crystal.
  • Precipitated crystalline In the context of the present invention, precipitated crystalline or crystalline is understood to mean solid forms according to the invention in an ordered crystalline structure.
  • precipitated non-crystalline In the context of the present invention, precipitated non-crystalline is understood to mean solid forms according to the invention in amorphous non-crystalline structures or states of aggregation and association.
  • dissolved form is understood to mean solid forms according to the invention which are dissolved or redissolved in a solution according to the invention.
  • Suspended form is defined as solid forms according to the invention which are suspended or resuspended in a solution according to the invention.
  • aqueous medium is understood as meaning water or mixtures of water with suitable inert solvents and other agents mentioned in example 1, such as, for example, buffers, stabilizers or auxiliaries, with the property that the aqueous media according to the invention alone do not precipitate or crystallize lead to the precipitation or crystallization until the addition of precipitation reagents according to the invention.
  • non-aqueous medium oils or mixtures of oils with water or other suitable inert Solvents and other agents mentioned in Example 1, such as stabilizers or auxiliaries, having the property that inventive non-aqueous media alone do not lead to the precipitation or crystallization of the antibody, but a precipitation or crystallization only by addition of erfindungsgennä.
  • biologically active means that anti-EGFR antibodies according to the invention are also converted into solid forms according to the invention and subsequent redissolution or resuspension their biological activity, in particular the binding to EGFR, inhibition of the binding of ligands, in particular EGF, to the EGFR, modulation, in particular inhibition of EGFR-mediated signal transduction and prophylaxis or therapy of EGFR-mediated diseases.
  • the high stability of the resulting crystals of antibodies according to the invention is also characterized in that it does not alter the Particle shape and the particle size spectrum, which would be considered critical with regard to immunogenic side effects, nor to changes in the primary structure or secondary structure of the protein comes.
  • the process according to the invention thus has the advantage that preferably the particle size of the crystals obtained can be controlled and preferably stable three-dimensional crystals are obtained.
  • the crystal size can be in the range of ⁇ m and nrn.
  • Anti-EGFR antibodies of the invention are preferably of monoclonal and murine or rumenan origin, more preferably of murine origin and chimeric or humanized.
  • the antibody directed against the receptor of the epidermal growth factor (EGFR) is particularly preferably Mab C225 (cetuximab) or Mab h425 (EMD 72000) and / or variants or fragments thereof.
  • Other antibodies directed against the EGFR are e.g. in EP0586002 and in J. Natl. Cancer Inst. 1993, 85: 27-33 (Mab 528).
  • Mab C225 (Cetuximab, Erbitux TM): Mab C225 (cetuximab) is a clinically proven antibody that binds to the EGF receptor. Mab C225 (cetuximab) is a chimeric antibody whose variable regions are murine and whose constant regions are of human origin. It was first reported by Naramura et al., Cancer immunol. Immunotherapy 1993, 37: 343-349 and WO 96/40210 Ai.
  • Mab h425 (EMD 72000) is a humanized monoclonal antibody (Mab) derived from murine anti-EGFR antibody 425 (Mab 425) (EP0531472).
  • the murine monoclonal antibody Mab 425 was developed in the human carcinoma cell line A431, as it binds here to an extracellular epitope of the epidermal growth factor receptor (EGFR). It has been found to inhibit the binding of EGF (Murthy et al., 1987). Increased expression of EGFR is found in malignant tissues from multiple sources, making Mab 425 a potential drug for the diagnosis and therapeutic treatment of human tumors.
  • Mab 425 was found to mediate tumor cytotoxicity in vitro, and to increase the tumor growth of epidermoid and colorectal cell lines in vitro
  • Mab 425 was shown to bind to xenografts of human malignant glioma in mice (Takahashi et al., 1987). Its humanized and chimeric forms are e.g. from EP0531472; Kettleborough et al., Protein Engineering 1991, 4: 773-783; Bier et al., Cancer Chemother Pharmacol.
  • Mab h425 (EMD 72000) is a humanized antibody (h425) in the clinical phase I / II whose constant region is composed of a K and a human ⁇ -1 chain (EP0531472).
  • Human anti-EGFR antibodies can be provided by XenoMouse technology as described in WO91 / 0741, WO9402602, WO9633735.
  • an antibody produced in clinical trials using this technology is also ABX-EGF
  • Antibody or immunoglobulin is used broadly within the scope of the present invention and is particularly concerned with polyclonal antibodies and multispecific antibodies (e.g., bispecific antibodies), and most preferably intact monoclonal antibodies (Mab) which are biologically active, as well as their variants and fragments. It is also heteroantibodies consisting of two or more antibodies or fragments thereof and / or various combinations thereof.
  • antibodies can be assigned to different "antibody (immunoglobulin) classes: IgA, IgD, IgE, IgG and IgM. Several of them can be subdivided further into subclasses (isotypes), for example IgG1, IgG2, IgG3, IgG4, IgA1 and IgA2. Antibodies usually have one
  • Monoclonal antibodies are obtained from a population of homogeneous cells. They are highly specific and directed against a single epitope, while polyclonal antibodies comprise different antibodies directed against different epitopes. Methods for producing monoclonal antibodies include, for example, those described by Kohler and Milstein (Nature 256, 495 (1975)) and in Burdon et al., (1985) "Monoclonal Antibody Technology, The Production and Characterization of Rodent and Human Hybridomas", Eds, Laboratory Techniques in Biochemistry and Molecular Biology, Volume 13, Elsevier Science Publishers, Amsterdam.
  • Monoclonal antibodies can also be isolated from phage antibody libraries using, for example, the techniques described in Clackson et al. (Nature, 352: 624-628 (1991)) and Marks et al. (J. Mol. Biol., 222: 58, 1-597 (1991)).
  • Variants (muteins) of antibodies are structurally related proteins, for example those which are modified by modification of the primary sequence (amino acid sequence), by glycoengineering (variants of the glycosylation sites or structures, also deglycosylated proteins), by PEGylation, by production can be obtained in modified host cells or by other techniques. Variants according to the invention are not limited to the above examples but include all variants of antibodies according to the invention known to the person skilled in the art.
  • Fragments (subsegments) of antibodies are cleavage products of antibodies which are obtained, for example, by limited enzymatic digestion using papain, pepsin and plasmin or by genetic engineering of the subsegments. Typical sub-segments are, for example, the bivalent F (ab ') 2 -
  • fragments according to the invention are not restricted to the above examples but comprise all fragments of antibodies according to the invention known to the person skilled in the art.
  • composition The terms pharmaceutical formulation and pharmaceutical preparation are used in the context of the present invention as synonyms.
  • pharmaceutically acceptable refers to pharmaceuticals, precipitation reagents, carriers, adjuvants, stabilizers, solvents, and other agents which enable administration of the pharmaceutical compositions obtained therefrom without undesirable physiological side effects such as nausea, dizziness, digestive problems, etc. to a mammal.
  • solid forms of anti-EGFR antibodies according to the invention preferably have the advantage that direct use is possible because physiologically harmless agents are used as precipitation reagents and thus no further use of the solid forms according to the invention in pharmaceutical formulations Cleaning steps for removing toxicologically harmful agents, such as high concentrations of organic solvents or other toxicologically harmful excipients, are required.
  • the preparation of solid forms of anti-EGFR antibodies according to the invention with preferably simultaneously high yield of native and pharmaceutically acceptable protein of high purity is preferably simple, time-saving and inexpensive.
  • the invention therefore also provides a process for producing a solid form of an anti-EGFR antibody according to the invention and / or one of its variants and / or fragments, which by dissolving or suspending in an aqueous medium leads to biologically active antibody protein, characterized in that in aqueous solution, dissolved or suspended antibodies and / or its variants and / or fragments are precipitated by a precipitation reagent and the precipitate is separated.
  • precipitation reagents in the process of the invention preferably polymers, more preferably polyethylene glycol (PEG), salts, more preferably ammonium sulfate, or organic solvents, more preferably ethanol used.
  • a solid form of an anti-EGFR antibody according to the invention can be prepared by adding to a solution containing antibodies according to the invention the precipitation reagents according to the invention mentioned in Example 1, preferably polymers, such as particularly preferred
  • Phosphate buffer in a concentration of 1 mM to 200 mM, preferably 2 to 20 mM, particularly preferably about 10 mM; also diluted with the addition of isotonizing agents such as potassium chloride, sodium chloride in a concentration of 1 - 1000 mM, preferably 40 mM - 310 mM) to the pre-calculated concentration.
  • isotonizing agents such as potassium chloride, sodium chloride in a concentration of 1 - 1000 mM, preferably 40 mM - 310 mM
  • precipitation reagents, auxiliaries, buffers and stabilizers according to the invention may also be added in solid form.
  • the solid forms of anti-EGFR antibodies according to the invention can be prepared by dissolving antibodies according to the invention first in water or in an aqueous solution containing one or more further ingredients, and subsequently with defined volumes of stock solutions containing the precipitation reagents, adjuvants, buffers and / or stabilizers mentioned in Example 1 in a defined concentration, are added.
  • precipitation reagents, auxiliaries, buffers and / or stabilizers according to the invention can also be added in a solid state of aggregation.
  • Antibodies according to the invention are also dissolved directly in a solution containing all precipitation reagents, auxiliaries, buffers and / or stabilizers.
  • the invention also encompasses all hydrates, salts and derivatives of the abovementioned agents known and conceivable to the person skilled in the art.
  • one or more of the agents mentioned in the invention may be added during or after completion of the precipitation process and optionally removed again, for example to carry out an additional purification step.
  • one or more of the precipitation reagents, adjuvants, buffers and / or stabilizers mentioned in the invention may be added during or after the completion of the preparation process of the antibody.
  • This may preferably be effected by dissolving the antibody according to the invention directly in an aqueous solution in the last step of the purification carried out after its preparation, which contains one, several or all further precipitation reagents, auxiliaries, buffers and / or stabilizers.
  • the respective further ingredient (s) need only be added in a lesser amount or not at all.
  • the respective ingredient in the last step of the purification of the antibody which follows its preparation in an aqueous solution containing all other precipitation, excipients, buffers and / or stabilizers is dissolved.
  • a directly to be filled or to be lyophilized solution can be obtained.
  • the resulting solution containing the respective antibody is adjusted to a pH of 4 to 10, preferably pH 5 to 9, sterile filtered and freeze-dried as required.
  • the reaction is carried out by methods known to those skilled in the art in a suitable solvent, in particular in an inert solvent.
  • suitable inert solvents are ethanol, glycerol, mixtures with water or pure or other auxiliaries, such as salts having a buffering or isotonic effect, containing water. Particularly preferred is water.
  • the process described here can be carried out particularly preferably in the "batch" format, solid forms of anti-EGFR antibodies according to the invention and / or variants and / or fragments thereof being particularly preferred by dissolving or suspending in an aqueous medium in biological
  • amorphous precipitates or crystals are obtained.
  • precipitates are obtained at a higher concentration of the precipitation reagent, whereas crystals are obtained at a lower concentration of the precipitation reagent.
  • concentration of the precipitation reagent, the concentration of the protein, the other agents according to the invention, the pH and the temperature, the reaction can be directed in the desired direction.
  • Exemplary crystallization conditions for Mab C225 (Erbitux TM) are listed in Examples 2 and 3, and Examples 4 and 5 show exemplary precipitation conditions.
  • the precipitation method according to the invention and the crystallization method according to the invention can also be combined.
  • Suitable reaction temperatures are at temperatures of -10 to 40 ° C, preferably at 0 to 25 ° C and most preferably at 4 to 20 ° C. Preference is given to working at a pressure of 1 to 20 bar, more preferably at atmospheric pressure. Preference is given to working at a pH of 4 to 10.
  • the duration of the reaction depends on the selected reaction conditions. In general, the reaction time is 0.5 hours to 10 days, preferably 1 to 24 hours, more preferably 2 to 12 hours.
  • Solvates of the solid forms according to the invention are understood to mean additions of inert solvent molecules to the solid forms according to the invention, which form due to their mutual attraction.
  • Solvates are, for example, hydrates, such as monohydrates or dihydrates or alkoxides, i. Addition compounds with alcohols such as with ethanol.
  • the resulting solid forms of anti-EGFR antibodies of the invention may be separated from the corresponding solution in which they are prepared (eg by centrifugation and washing) and may be stored after separation in another composition, or they may be directly incorporated in the Remain manufacturing solution.
  • the resulting solid forms of the invention can also be incorporated into desired solvents for use.
  • the solid forms of anti-EGFR antibodies according to the invention are preferably biologically active after reconstitution or resuspension, and in the method according to the invention it is preferable not to denature the antibodies. Thus, the biological activity of the protein is preferably maintained.
  • inventive solid forms of anti-EGFR antibodies to prepare pharmaceutical formulations.
  • These formulations preferably have a higher stability to physicochemical influences such as, for example, oxidation, mechanical stresses, unfavorable pH values and temperatures than conventional protein solutions of antibodies.
  • comparable stability can otherwise only be achieved by costly and time-consuming methods, such as the addition of stabilizers, cool storage, freezing or freeze-drying.
  • the high stability preferably enables easier and cheaper storage, transport and production of pharmaceutically valuable formulations, such as ready-to-use formulations, sustained-release formulations or controlled release formulations over an extended period of time.
  • Another object of the invention are therefore solid forms of anti-EGFR antibodies according to the invention as storage-stable drugs.
  • a particularly preferred subject of the invention are also pharmaceutical preparations containing at least one solid form of an anti-EGFR antibody according to the invention in precipitated non-crystalline, precipitated crystalline or in dissolved or suspended form, and optionally excipients and / or adjuvants and / or other pharmaceutical agents.
  • compositions according to the invention may contain solid forms according to the invention in precipitated form, ie as precipitate or as crystal, or in redissolved or resuspended form.
  • the invention therefore also relates to pharmaceutical preparations comprising at least one precipitate and / or crystal of an anti-EGFR antibody, preferably of an anti-EGFR monoclonal antibody, more preferably of Mab C225 (cetuximab) or Mab h425 (EMD 72000) and / or their variants or fragments in precipitated, redissolved or suspended form and optionally excipients and / or excipients and / or other active pharmaceutical ingredients.
  • an anti-EGFR antibody preferably of an anti-EGFR monoclonal antibody, more preferably of Mab C225 (cetuximab) or Mab h425 (EMD 72000) and / or their variants or fragments in precipitated, redissolved or suspended form and optionally excipients and / or excipients and / or other active pharmaceutical ingredients
  • the solid forms of anti-EGFR antibodies of the invention make it possible to produce highly concentrated formulations without unfavorable undesired aggregation of the antibodies of the invention or to undesirably high viscosity, as can be observed in common, highly concentrated protein solutions.
  • solid forms of anti-EGFR antibodies according to the invention with aqueous solvents or in aqueous media, ready-to-use solutions with a high proportion of active ingredient can be redissolved or resuspended.
  • Recently, more highly concentrated formulations of protein active ingredients are increasingly required.
  • Most antibodies used for therapy are dosed in mg / kg.
  • a high dose and low volumes to be administered demonstrate the need for highly concentrated protein preparations at concentrations greater than 100 mg / ml.
  • highly concentrated protein formulations may be used in preclinical tests to investigate safety and efficacy in vitro and in vivo (on the animal model), in clinical tests for safety and efficacy in humans, and in clinical use of the product (especially subcutaneous Application) have significant advantages. Their advantages are, in particular, that a smaller volume of the preparation must be used. in the
  • Subcutaneous administration of protein drugs may have several causes.
  • a specific targeting associated with a "therapeutic window" may be desired subcutaneous administration the advantage that the patient can make the application itself, without having to rely on medical staff.
  • the example of insulin clearly shows these benefits.
  • the injections for subcutaneous administration can amount to a maximum of 1-1.5 ml, highly concentrated protein formulations with more than 100 mg / ml are often necessary.
  • a highly concentrated stable antibody formulation can be obtained which, upon resuspension or reconstitution, has a reduced viscosity compared to liquid antibody formulations of the same concentration and thereby facilitates handling in parenteral administration.
  • the invention therefore also relates to pharmaceutical preparations containing at least one solid form of an anti-EGFR antibody according to the invention and / or one of its variants and / or
  • the invention also provides a process for preparing a highly concentrated pharmaceutical preparation according to the invention, which comprises dissolving or resuspending at least one solid form of an anti-EGFR antibody according to the invention and / or one of its variants and / or fragments in a solution according to the invention and Antibody concentration is preferably 10-200 mg / ml, more preferably 50-150 mg / ml.
  • Aqueous preparations may be prepared by dissolving or suspending solid forms of anti-EGFR antibodies of the invention in an aqueous solution and adding adjuvants if desired.
  • this is a solution or suspension with a defined concentration of solid forms according to the invention with defined volumes of stock solutions containing said other auxiliaries in a defined concentration, and optionally diluted with water to the pre-calculated concentration.
  • the adjuvants may be added in solid form.
  • Solution or suspension can then be mixed with the respectively required amounts of stock solutions and / or water.
  • solid forms of anti-EGFR antibodies according to the invention can also be dissolved or suspended directly in a solution containing all further excipients.
  • Antibody-containing solutions or suspensions having a pH of 4 to 10, preferably having a pH of 5 to 9, and an osmolality of 250 to 350 mOsmol / kg can advantageously be prepared from the solid forms according to the invention by reconstitution with aqueous solvents , ,
  • the resuspended or reconstituted preparation can thus be administered largely painlessly intravenously, intraarterially and also subcutaneously directly.
  • the preparation can also infusion solutions, such. Glucose solution, isotonic saline or Ringer's solution, which may also contain other active ingredients, are added, so that even larger amounts of active ingredient can be applied.
  • compositions according to the invention may also contain mixtures of precipitates and / or crystals according to the invention in precipitated or crystalline and / or redissolved or resuspended form.
  • the preparations of the invention are physiologically well tolerated, easy to prepare, precisely metered and preferably over the duration of storage and transport and in multiple freezing and thawing processes in terms of content, decomposition products and Aggregates stable. They may be stored stably at a refrigerator temperature (2-8 ° C) and at room temperature (23-27 ° C) and 60% relative humidity (RH), preferably for a period of at least three months to two years. Surprisingly, the preparations according to the invention are preferably also higher
  • Temperatures and humidities for example, at a temperature of 40 ° C and 75% relative humidity for at least six months stable storage.
  • solid forms according to the invention can be stably stored by drying and, if necessary, converted by solution or suspension into a ready-to-use pharmaceutical preparation.
  • Possible methods of drying include, but are not limited to, nitrogen gas drying, vacuum oven drying, lyophilization, organic solvent washing and subsequent air drying, fluid bed drying, fluidized bed drying, spray drying, drum drying, layer drying; Air drying at room temperature and other methods.
  • the term "effective amount” means the amount of a drug or pharmaceutical agent which elicits a biological or medical response in a tissue, system, animal or human, e.g. sought or desired by a researcher or physician.
  • terapéuticaally effective amount means an amount which, compared to a corresponding subject who has not received this amount, results in: improved curative treatment, cure, prevention or elimination of a disease, a disease, a disease state, suffering, interference or prevention of side effects, or even reducing the progression of a disease, condition or disorder.
  • therapeutically effective amount also includes the amounts effective to increase normal physiological function.
  • Drugs may be presented in the form of dosage units containing a predetermined amount of active ingredient per unit dose.
  • a moiety may contain, for example, 0.5 mg to 1 g, preferably 1 mg to 800 mg, of an active ingredient according to the invention, depending on the condition being treated, the route of administration and the age, weight and condition of the patient.
  • Preferred unit dosage formulations are those containing a daily or partial dose as indicated above or a corresponding fraction thereof of an active ingredient.
  • such drugs can be prepared by any of the methods well known in the pharmaceutical art.
  • Drugs may be administered by any suitable route, for example oral (including buccal or sublingual), rectal, pumonal, nasal, topical (including buccal, sublingual or transdermal), vaginal or parenteral (including subcutaneous, intramuscular, intravenous or intradermal ) Ways, adapt.
  • Such drugs can be prepared by any method known in the pharmaceutical art, such as by bringing the drug into association with the carrier (s) or excipient (s).
  • parenteral administration for administration of the medicaments according to the invention, preferably parenteral administration is suitable.
  • parenteral administration intravenous and subcutaneous administration are particularly preferred.
  • intravenous administration the injection can be made directly or as an addition to infusion solutions.
  • drugs are suitable for subcutaneous administration, since using solid forms of anti-EGFR antibodies according to the invention stable, highly concentrated Forumlierungen produce. As a result, it is possible to realize the highly concentrated formulations and small volumes to be applied, which are required for the par- ental or subcutaneous administration.
  • Subcutaneous administration has the advantage that the patient can administer the drug himself without medical expert assistance.
  • Solid forms of anti-EGFR antibodies according to the invention are also suitable for the preparation of medicaments to be administered parenterally with controlled, uniform and / or delayed release of active ingredient (slow-release, sustained-release, controlled release).
  • the precipitates and / or crystals according to the invention are preferably present in suspension and dissolve over a prolonged and / or controlled period of time and are then present in the native and active state.
  • solid forms of anti-EGFR antibodies according to the invention are also suitable for the production of depot formulations which are advantageous for the patient, since an application is necessary only at relatively long time intervals.
  • Pharmaceutical compositions according to the invention can also be injected directly into the tumor and thus exert their effect directly on the intended site of action.
  • Medicaments adapted for parenteral administration include aqueous and non-aqueous sterile injection solutions containing the antioxidants, buffers, bacteriostats and solutes, by which the antioxidants, buffers, bacteriostats and solutes, by which the antioxidants, buffers, bacteriostats and solutes, by which the antioxidants, buffers, bacteriostats and solutes, by which the antioxidants, buffers, bacteriostats and solutes, by which the
  • Formulation is made isotonic with the blood of the recipient to be treated included; and aqueous and non-aqueous sterile suspensions which may contain suspending agents and thickeners.
  • the formulations may be presented in single or multi-dose containers, such as sealed vials and vials, and stored in freeze-dried (lyophilized) condition such that only the addition of the sterile carrier liquid, eg water for injections, is required immediately before use.
  • Injection solutions and suspensions prepared by formulation can be prepared from sterile powders, granules and tablets.
  • the solid forms of anti-EGFR antibodies according to the invention can also be administered in the form of liposome delivery systems, such as e.g. small unilamellar vesicles, large unilamellar vesicles and multilamellar vesicles.
  • liposomes can be prepared from various phospholipids, such as e.g. Cholesterol, stearylamine or phosphatidylcholines.
  • the solid forms of anti-EGFR antibodies according to the invention and their variants in precipitated, redissolved or suspended form can also be coupled with soluble polymers as targeted drug carriers.
  • soluble polymers may include polyvinylpyrrolidone, pyran copolymer, polyhydroxypropylmethacrylamidephenol, polyhydroxyethylaspartamidephenol or polyethyleneoxidepolylysine substituted with palmitoyl radicals.
  • the solid forms of anti-EGFR antibodies can be linked to a class of biodegradable polymers suitable for the controlled release of a drug, e.g.
  • Polylactic acid Polyepsilon-caprolactone, polyhydroxybutyric acid, polyorthoesters, polyacetals, polydihydroxypyrans, polycyanoacrylates, polylactic acid, polymers such as conjugates between dextran and methacrylates, polyphosphoesters, various polysaccharides and polyamines and poly- ⁇ -caprolactone, albumin, chitosan, collagen or modified gelatin and crosslinked or amphipathic block copolymers of hydrogels.
  • Drugs adapted for transdermal administration may act as discrete plasters for prolonged, intimate contact with the epidermis of the epidermis Be presented to the recipient.
  • the drug may be delivered from the patch by iontophoresis as generally described in Pharmaceutical Research, 3 (6), 318 (1986).
  • Drugs adapted for topical administration may be described as
  • Ointments creams, suspensions, lotions, powders, solutions, pastes, gels, sprays, aerosols or oils.
  • the formulations are preferably applied as a topical ointment or cream.
  • the active ingredient can be used with either a paraffinic or water miscible cream base.
  • the active ingredient can be formulated into a cream with an oil-in-water cream base or a water-in-oil base.
  • the medicaments adapted for topical application to the eye include eye drops, the active ingredient being dissolved or suspended in a suitable carrier, in particular an aqueous solvent.
  • Drugs adapted for rectal administration may be presented in the form of suppositories or enemas.
  • Drugs adapted for administration by inhalation include fine particulate dusts or mists which may be generated by various types of pressurized dosing dispensers with aerosols, nebulizers or insufflators. Particularly preferred within the scope of the present invention are powders of solid forms of anti-EGFR antibodies according to the invention for application as inhalant. Pharmaceutical drugs adapted for vaginal administration may be presented as pessaries, tampons, creams, gels, pastes, foams or spray formulations.
  • compositions according to the invention in addition to the above-mentioned particularly mentioned constituents, may contain other agents customary in the art with respect to the respective type of pharmaceutical formulation.
  • kits consisting of separate packages of a) an effective amount of a precipitate and / or a crystal of an anti-EGFR antibody, preferably an anti-EGFR monoclonal antibody, particularly preferably Mab C225 (Cetuximab ) or Mab h425 (EMD 72000) and / or their variants or fragments in precipitated, redissolved or suspended form, and b) an effective amount of another active pharmaceutical ingredient.
  • an anti-EGFR antibody preferably an anti-EGFR monoclonal antibody, particularly preferably Mab C225 (Cetuximab ) or Mab h425 (EMD 72000) and / or their variants or fragments in precipitated, redissolved or suspended form
  • an effective amount of another active pharmaceutical ingredient preferably an effective amount of another active pharmaceutical ingredient.
  • the kit contains suitable containers, such as boxes or boxes, individual bottles, bags or ampoules.
  • the set may e.g. containing separate ampoules, in each of which an effective amount of a solid form according to the invention, optionally dissolved in redissolved form and an effective amount of another drug or in lyophilized form.
  • a therapeutically effective amount of a solid form of anti-EFGR antibody of the invention will depend on a number of factors, including e.g. the age and weight, the exact condition of the disease requiring treatment, its severity, the nature of the formulation, and
  • an effective amount is one anti-EFGR antibody according to the invention for the treatment of neoplastic growth, eg colon or breast carcinoma, generally in the range of 0.1 to 100 mg / kg body weight of the recipient (mammal) per day and more typically in the range of 1 to 10 mg / kg of body weight per day.
  • the actual amount per day would usually be between 70 and 700 mg, this amount as a single dose per day or more commonly in a number of divided doses (such as two, three, four, five or six) per Day can be given so that the total daily dose is the same.
  • the determination of the appropriate antibody titer is carried out by methods known to the person skilled in the art.
  • the dosage to be administered is generally sufficient to achieve the desired antitumor effect. However, the dosage should also be as low as possible, so that no side effects such as undesirable cross-reactions, anaphylactic reactions or the like occur.
  • Another object of the invention is the use of solid forms according to the invention for the manufacture of a medicament which contains the biologically active antibody and / or one of its variants and / or
  • Medicaments according to the invention can be used in particular for the prophylaxis and / or treatment of diseases and disease states.
  • the invention therefore relates to the use of solid forms of anti-EGFR antibodies according to the invention, preferably of monoclonal anti-EGFR antibodies, more preferably of Mab C225 (cetuximab) or Mab h425 (EMD 72000) and / or variants or fragments thereof precipitated, redissolved or suspended form for the manufacture of a medicament for the treatment and / or prophylaxis of diseases.
  • Medicaments containing solid forms of the antibodies of the invention in redissolved or suspended form can effectively regulate, modulate or inhibit EGFR and can therefore be used to prevent and / or treat diseases associated with unregulated or impaired EGFR activity.
  • the solid forms of anti-EGFR antibodies according to the invention can therefore be used in the treatment of certain types of cancer, as well as pathological angiogenesis-related diseases such as diabetic retinopathy or inflammation.
  • Another object of the invention is therefore the use of solid forms according to the invention in precipitated, redissolved or resuspended form for the manufacture of a medicament for the treatment and / or prophylaxis of diseases caused by EGFR and / or by EGFR-mediated signal transduction, mediated and / or be propagated.
  • Medicaments according to the invention are particularly suitable for the treatment and / or prophylaxis of cancer, including solid carcinomas, such as carcinomas (eg of the lungs, the pancreas, the thyroid, the bladder or the colon), myeloid diseases (eg. myeloid leukemia) or adenomas (eg villous colon adenoma), pathological carcinomas, such as carcinomas (eg of the lungs, the pancreas, the thyroid, the bladder or the colon), myeloid diseases (eg. myeloid leukemia) or adenomas (eg villous colon adenoma), pathological
  • solid carcinomas such as carcinomas (eg of the lungs, the pancreas, the thyroid, the bladder or the colon)
  • myeloid diseases eg. myeloid leukemia
  • adenomas eg villous colon adenoma
  • the drugs are also useful in the treatment of complement activation-dependent Chronic inflammation (Niculescu et al. (2002) Immunol. Res., 24: 191-199) and immunodeficiency induced by HIV-1 (human immunodeficiency virus type 1) (Popik et al., (1998) J Virol, 72: 6406- 6413).
  • compositions are suitable as pharmaceutical
  • EGFR-related diseases refers to pathological conditions that depend on EGFR activity.
  • EGFR is involved either directly or indirectly in the signal transduction pathways of various cellular activities, including proliferation, adhesion and migration as well as differentiation associated with EGFR activity include proliferation of tumor cells, pathological neovascularization that promotes solid tumor growth, neovascularization in the eye (diabetic retinopathy, age-related macular degeneration, and the like), and inflammation (psoriasis, rheumatoid arthritis, and the like).
  • the diseases discussed here are divided into two groups, hyperproliferative and non-hyperproliferative diseases.
  • psoriasis, arthritis, inflammation, endometriosis, scarring, benign prostatic hyperplasia, immunological diseases, autoimmune diseases and immunodeficiency diseases are considered to be noncancerous diseases of which arthritis, inflammation, immunological diseases, autoimmune diseases and immunodeficiency diseases are usually considered to be non-hyperproliferative diseases .
  • Brain cancer, lung cancer, squamous cell cancer, bladder cancer, gastric cancer, pancreatic cancer, liver cancer, kidney cancer, colorectal cancer, breast cancer, head cancer, cervical cancer, esophageal cancer, gynecological cancer, thyroid cancer, lymphoma, chronic leukemia and acute leukemia are both cancerous To look at diseases, all of which are usually counted among the group of hyperproliferative disorders.
  • cancerous cell growth, and in particular cancerous cell growth mediated directly or indirectly by EGFR is a disease that is an object of the present invention.
  • the drugs according to the invention have an in vivo antiproliferative action in a xenograft tumor model.
  • the medicaments of the invention are administered to a patient with a hyperproliferative disorder, e.g. To inhibit tumor growth, to reduce inflammation associated with lymphoproliferative disease, to inhibit graft rejection or neurological damage due to tissue repair, etc.
  • the present pharmaceutical compositions are useful for prophylactic or therapeutic purposes.
  • the term "treat” is used to refer to both the prevention of disease and the treatment of pre-existing conditions
  • the prevention of proliferation is accomplished by administering the subject medicines prior to developing the evident disease, e.g., to prevent tumor growth , Prevention of metastatic growth, reduction of cardiovascular surgery-related restenosis, etc.
  • the drugs are used to treat persistent diseases by stabilizing or ameliorating the clinical symptoms of the patient.
  • the host or patient may be of any mammalian species, e.g. A primate species, especially humans; Rodents, including mice, rats and hamsters; Rabbit; Horses, cattle, dogs, cats, etc. Animal models are of interest for experimental studies, providing a model for the treatment of human disease.
  • the susceptibility of a particular cell to treatment with the medicaments of the invention can be determined by in vitro tests. Typically, a culture of the cell is incubated with a drug of the invention at various concentrations for a time sufficient to allow the drugs to induce cell death or inhibit migration, usually between about one hour and one week. Cultured cells from a biopsy sample can be used for in vitro assays. The viable cells remaining after treatment are then counted.
  • the dose will vary depending on the specific drugs used, the specific disease, the patient status, etc. Typically, a therapeutic dose will be sufficient to substantially reduce the unwanted cell population in the target tissue while maintaining the viability of the patient. Treatment is generally continued until there is a significant reduction, e.g. B. at least about 50% reduction in the specific cell count and can be continued until essentially no more unwanted cells are detected in the body.
  • Non-radioactive ELISA assay methods use specific phospho-antibodies (Phospho-AK).
  • Phospho-AK binds only the phosphorylated substrate. This binding is detectable by chemiluminescence with a second peroxidase-conjugated anti-sheep antibody (Ross et al., 2002, Biochem J., just prior to publication, manuscript BJ20020786).
  • the diseases and conditions that can be treated, prevented or alleviated by the present invention include, but are not limited to, the diseases and conditions listed below.
  • the pharmaceutical compositions of the invention are useful in the treatment and / or prophylaxis of a variety of diseases and conditions in which proliferation and / or migration of smooth muscle cells and / or inflammatory cells into the intimal layer of a vessel is present, resulting in limited perfusion of this vessel, e.g. In neointimal occlusive lesions.
  • Occlusive transplant vascular diseases of interest include atherosclerosis, coronary vascular disease after transplantation, vein graft stenosis, peri-anastomotic prosthetic restenosis, restenosis after angioplasty or stent placement, and the like.
  • the present invention encompasses the use of the medicaments of the invention for the treatment or prevention of cancer.
  • a particularly preferred subject matter of the invention is therefore the use of solid forms of anti-EGFR antibodies according to the invention for the preparation of a medicament for the treatment and / or
  • the tumor is particularly preferably selected from the group consisting of Brain tumor, tumor of the urogenital tract, tumor of the lymphatic system, gastric tumor, laryngeal tumor, monocytic leukemia, lung adenocarcinoma, small cell lung carcinoma, pancreatic cancer, glioblastoma and breast carcinoma, but not limited thereto.
  • Another object of the invention is the use of medicaments according to the invention for the manufacture of a medicament for the treatment of diseases selected from the group of cancerous diseases consisting of squamous cell cancer, bladder cancer, gastric cancer, liver cancer, kidney cancer, colorectal cancer, breast cancer, head cancer, cervical cancer, Esophageal cancer, gynecological cancer, thyroid cancer, lymphoma, chronic leukemia and acute leukemia.
  • diseases selected from the group of cancerous diseases consisting of squamous cell cancer, bladder cancer, gastric cancer, liver cancer, kidney cancer, colorectal cancer, breast cancer, head cancer, cervical cancer, Esophageal cancer, gynecological cancer, thyroid cancer, lymphoma, chronic leukemia and acute leukemia.
  • the medicaments of the invention may be administered to patients for the treatment of cancer.
  • the present drugs inhibit tumor angiogenesis and thus affect the growth of tumors (Rak, J. Rak et al., Cancer Research, 55: 4575-4580, 1995).
  • the angiogenesis-inhibiting properties of the pharmaceutical compositions of the invention are also useful in the treatment of certain forms of blindness associated with retinal neovascularization.
  • the invention therefore further provides the use of solid forms of anti-EGFR antibodies according to the invention in precipitated, redissolved or suspended form for the preparation of a medicament for the treatment and / or prophylaxis of diseases caused, mediated and / or propagated by angiogenesis.
  • angiogenesis is an ocular disease such as retinal vascularization, diabetic retinopathy, age-related macular degeneration, and the like.
  • the invention therefore also relates to the use of solid forms of anti-EGFR antibodies according to the invention in precipitated, redissolved or suspended form for the preparation of a medicament for the treatment and / or prophylaxis of diseases selected from the group consisting of retinal vascularization, diabetic retinopathy , age-related macular degeneration and / or inflammatory diseases.
  • Another object of the invention is the use of medicaments according to the invention for the treatment and / or prophylaxis of diseases selected from the group consisting of psoriasis, rheumatoid arthritis, contact dermatitis, late-type hypersensitivity reaction, inflammation, endometriosis, scarring, benign prostatic hyperplasia, immunological diseases , Autoimmune diseases and immunodeficiency diseases.
  • diseases selected from the group consisting of psoriasis, rheumatoid arthritis, contact dermatitis, late-type hypersensitivity reaction, inflammation, endometriosis, scarring, benign prostatic hyperplasia, immunological diseases , Autoimmune diseases and immunodeficiency diseases.
  • the invention also provides the use of medicaments according to the invention for the treatment and / or prophylaxis of bone pathologies selected from the group consisting of osteosarcoma, osteoarthritis and rickets.
  • compositions of the invention may be used to provide additive or synergistic effects in certain existing cancer chemotherapies and radiation and / or may be used to restore the efficacy of certain existing cancer chemotherapies and radiation.
  • the invention therefore also relates to the use of solid forms of anti-EGFR antibodies according to the invention in precipitated, redissolved or suspended form for the preparation of a medicament for the treatment and / or prophylaxis of diseases, wherein a therapeutically effective amount of an inventive solid form in precipitated, redissolved or suspended form in combination with a compound from the group 1) estrogen receptor modulator, 2) androgen receptor modulator, 3) retinoid receptor modulator, 4) cytotoxic agent, 5) antiproliferative agent, 6) prenyl protein transferase inhibitors, 7) HMG-CoA reductase inhibitors, 8) HIV protease
  • Inhibitors 9) reverse transcriptase inhibitors, 10) growth factor receptor inhibitors and 11) angiogenesis inhibitors.
  • the invention therefore also relates to the use of solid forms of anti-EGFR antibodies according to the invention in precipitated, redissolved or suspended form for the preparation of a medicament for the treatment and / or prophylaxis of diseases, wherein a therapeutically effective amount of a solid form according to the invention is precipitated, redissolved or suspended form in combination with radiotherapy and a compound from group 1) estrogen receptor modulator, 2) androgen receptor modulator, 3) retinoid receptor modulator, 4) cytotoxic agent, 5) antiproliferative agent, 6) prenyl protein transferase inhibitors, 7) HMG-CoA reductase 8) HIV protease inhibitors, 9) reverse transcriptase inhibitors, 10) growth factor receptor inhibitors, and 11) angiogenesis inhibitors.
  • compositions of the present invention may also be co-administered with other well-known therapeutics selected for their particular suitability for the condition being treated.
  • the antiresorptive bisphosphonates such as alendronate and risedronate, integrin blockers (as defined below) such as ⁇ v ⁇ 3 antagonists, conjugated estrogens used in hormone therapy such as Prempro®, Premarin® and Endometrion®; selective
  • Estrogen receptor modulators such as raloxifene, droloxifene, CP- 336.156 (Pfizer) and lasofoxifene, cathepsin K inhibitors and ATP proton pump inhibitors.
  • the present pharmaceutical compositions are also suitable for combination with known anticancer agents.
  • known anticancer agents include the following: estrogen receptor modulators, androgen receptor modulators, retinoid receptor modulators, cytotoxic agents, antiproliferative agents, prenyl-protein transferase inhibitors, HMG-CoA reductase inhibitors, HIV protease inhibitors, reverse transcriptase inhibitors, growth factor inhibitors, and angiogenesis inhibitors ,
  • the present compounds are particularly suitable for co-administration with radiotherapy.
  • Estrogen receptor modulators refers to compounds that interfere with or inhibit the binding of estrogen to the receptor, regardless of how this occurs.
  • the estrogen receptor modulators include, for example, tamoxifen, raloxifene, idoxifen,
  • LY353381 LY 117081, toremifene, fulvestrant, 4- [7- (2,2-dimethyl-1-oxopropoxy-4-methyl-2- [4- [2- (1-piperidinyl) ethoxy] phenyl] -2H-1 benzopyran-3-yl] phenyl-2,2-dimethylpropanoate, 4,4'-dihydroxybenzophenone-2,4-dinitrophenylhydrazone and SH646, but this is not intended to be limiting.
  • Androgen receptor modulators refers to compounds that interfere with or inhibit the binding of androgens to the receptor, regardless of how this occurs, and the androgen receptor modulators include, for example, finasteride and other 5 ⁇ -reductase inhibitors, nilutamide, flutamide, bicalutamide , Liarozole and abiraterone acetate.
  • Retinoid receptor modulators refers to compounds that interfere with or inhibit the binding of retinoids to the receptor, regardless of how this occurs.
  • retinoid receptor modulators include, for example, bexarotene, tretinoin, 13-cis-retinoic acid, 9- cis-retinoic acid, ⁇ -difluoromethylmuthine, ILX23-7553, trans-N- (4'-hydroxyphenyl) -retinamide and N-4-carboxyphenylretinamide.
  • Cytotoxic agents refers to compounds that cause cell death or interfere with cell myosis, primarily through direct action on cell function, including alkylating agents, tumor necrosis factors, intercalators, microtubulin inhibitors, and topoisomerase inhibitors.
  • the cytotoxic agents include, for example, tirapazimine, sertenef, cachectin, ifosfamide, tasonermine, lonidamine, carboplatin, altretamine, prednimustine, dibromodulcite, ranimustine, fotemustine, nedaplatin, oxaliplatin, temozolomide, heptaplatin, estramustine, improvisulfanosylate, trofosfamide, nimustine, Dibrospidium chloride, Pumitepa, Lobaplatin, Satraplatin, Profiromycin,
  • Mitoxantrone pirarubicin, pinafid, valrubicin, amrubicin, antineoplaston, 3'-desamino-3'-morpholino-13-desoxo-10-hydroxycarminomycin, annamycin, galarubicin, elinafide, MEN10755 and 4-desmethoxy-3-desamino-3-aziridinyl 4-methylsulfonyldaunorubicin (see WO 00/50032), but this is not intended to be limiting.
  • microtubulin inhibitors include, for example, paclitaxel, vindesine sulfate, S '' '- dideshydro' '- deoxy- ⁇ '-norvincaleukoblastin, docetaxol, rhizoxin, dolastatin, mivobulinisethionate, auristatin, cemadotin, RPR109881, BMS184476, vinflunine, Cryptophycin, 2,3,4,5,6-pentafluoro-N- (3-fluoro-4-methoxyphenyl) benzenesulfonamide, anhydrovinblastine, N, N-dimethyl-L-valyl-L-valyl-N-methyl-L-valyl -L-prolyl-L-proline t-butylamide, TDX258 and BMS188797.
  • paclitaxel vindesine sulfate
  • Topoisomerase inhibitors are, for example, topotecan, hycaptamine, irinotecan, rubitecan, ⁇ -ethoxypropionyl-S '-' - O-exo-benzylidene-chartreusine, 9-methoxy-N, N-dimethyl-5-nitropyrazolo [3,4,5 ⁇ kl] acridine-2
  • Antiproliferative agents include antisense RNA and DNA oligonucleotides such as G3139, ODN698, RVASKRAS, GEM231, and INX3001, and antimetabolites such as enocitabine, carmofur, tegafur, pentostatin, doxifluridine, trimetrexate, fludarabine, capecitabine, galocitabine, cytarabine.
  • antiproliferative agents also include other monoclonal antibodies against growth factors than already among the "Angiogenesis inhibitors” such as trastuzumab, as well as tumor suppressor genes such as p53, can be delivered via recombinant virus-mediated gene transfer (see, eg, US Patent No. 6,069,134.)
  • Drugs of the invention may also be used in combination with any other therapeutic antibody known to those skilled in the art or administered in conjunction with the above-mentioned diseases suitable pharmaceutical agents.
  • solid forms according to the invention of the invention may be used in precipitated, redissolved or suspended form for the isolation and for the study of the activity or expression of EGFR.
  • they are particularly suitable for use in diagnostic procedures for diseases associated with unregulated or impaired EGFR activity.
  • Another object of the invention is therefore the use of solid forms of anti-EGFR antibodies according to the invention in precipitated, redissolved or suspended form as activators or inhibitors of EGFR, particularly preferred as inhibitors of EGFR.
  • antibodies according to the invention can be radiolabeled, for example.
  • a preferred labeling method is the iodogen method (Fraker et al., 1978).
  • the antibody is particularly preferably used as F (ab ') 2 fragment. This results in excellent results, so no background subtraction is necessary.
  • fragments can be prepared by known methods (e.g., Herlyn et al., 1983). In general, pepsin digestion is performed at acid pH and the fragments are separated from undigested IgG and heavy chain fragments by protein A-Sepharose TM chromatography.
  • the precipitates and / or crystals of the present invention preferably exhibit beneficial biological activity that is useful in enzyme assays, such as those described in U.S. Pat Examples described, easily detectable.
  • the antibodies of the invention preferably exhibit and effect an inhibiting effect, usually documented by IC 50 values in a suitable range, preferably in the micromolar range, and more preferably in the nanomolar range.
  • Detection Methods With respect to detection methods, the present invention encompasses all detection methods known to those skilled in the art or from the literature.
  • Crystalline structures can be detected, for example, by means of diffraction spectra in the X-ray diffraction measurement.
  • crystalline structures of antibodies according to the invention can be detected by microscopic examinations using polarization filters.
  • Other detection methods include, but are not limited to, electron micrographs.
  • Proofs of protein size, structural integrity, purity or glycosylation patterns of the solid forms of the invention in precipitated, redissolved or resuspended form include, but are not limited to, SE-HPLC, peptide mapping (digestion), N-terminal sequencing, SDS-Page, Tris -Glycine gradient gel (non-reducing), FTIR method (Fourier transform infrared spectra), CD (circular dichroism), RAMAN spectroscopy, carbohydrate staining (PAS method), oligosaccharide profiling, determination of monosaccharide
  • composition or isoelectric focusing Composition or isoelectric focusing.
  • the stability of solid forms or formulations according to the invention can be determined, for example, but not limited to, by using stability programs, eg storage at 25 ° C. and 60% relative humidity and at 40 ° C. and 70% relative humidity over an extended period of time and Determination of stability or structural integrity of the protein at regular intervals, for example by the above-mentioned detection methods (SE-HPLC, FT-1R, SDS-PAGE (reducing or non-reducing)).
  • stability programs eg storage at 25 ° C. and 60% relative humidity and at 40 ° C. and 70% relative humidity over an extended period of time and Determination of stability or structural integrity of the protein at regular intervals, for example by the above-mentioned detection methods (SE-HPLC, FT-1R, SDS-PAGE (reducing or non-reducing)).
  • Detection methods for biological activity or efficacy of solid forms according to the invention in precipitated crystalline, precipitated, non-crystalline, dissolved or suspended form include, but are not limited to, ELISA, biological cell assays, FTIR or CD.
  • Crystal or precipitate size include, but are not limited to, visual control, sub-particle analysis , Nephelometry or Turbidimetry, Dynamic Light Scattering Characterization.
  • “usual work-up” means adding water if necessary and, if necessary, adjusting to pH values between 2 and 10, depending on the constitution of the final product.
  • Example 1 Agents According to the Invention and Precipitation and / or Crystallization Conditions
  • Precipitation Reagents 1.1 Salts: eg sodium acetate trihydrate :, potassium chloride :, sodium chloride :, tri-sodium citrate dihydrate :, di-potassium hydrogen phosphate :, di-sodium hydrogen phosphate dihydrate :, ammonium sulfate :, ammonium acetate, ammonium bromide, ammonium chloride, tri-ammonium citrate, di-ammonium hydrogen citrate, ammonium dihydrogen phosphate, di-ammonium hydrogen phosphate, di-ammonium tartrate, citric acid monohydrate, imidazole, potassium acetate, potassium bromide, tri-potassium citrate monohydrate, potassium dihydrogen phosphate, potassium sulfate, agnesium acetate tetrahydrate, magnesium bromide hexahydrate, magnesium chloride hexahydrate, magnesium sulfate heptahydrate, sodium dihydrogen phosphate monohydrate, sodium
  • Cyclodextrins Dextran, Sodium Carboxymethylcellulose (Na-CMC), Poloxamer, Polyacrylic Acid, Polyethylene Glycol Monomethyl Ether (mPEG), Polypropylene Glycol (PPG), Polyvinyl Alcohol (PVA), Polyvinylpyrrolidone (PVP)
  • Organic solvents e.g. Ethanol, glycerol
  • Excipients e.g. Viscosity modifiers, detergents (e.g., Tween®, Solutol® HS 15, Pluronic®, Cremophor® EL, Avacel® 20, polyoxyethylene sorbitan monooleate), reducing agents (glutathione, 2-mercaptoethanol, dithiothreitol), chelating agents (EDTA, EGTA)
  • detergents e.g., Tween®, Solutol® HS 15, Pluronic®, Cremophor® EL, Avacel® 20, polyoxyethylene sorbitan monooleate
  • reducing agents glutathione, 2-mercaptoethanol, dithiothreitol
  • chelating agents EDTA, EGTA
  • Buffer eg phosphate buffer: Na (or K) phosphate; also with addition of isotization agent (eg Na (or K) CI), possible pH about 6.0 to 8.2
  • Citrate buffer Na citrate or citric acid, possible pH approx. 2.2 - 6.5 ;. also with addition of isotonizing agents (eg NaCl); Other salts are conceivable for isotonization.
  • Succinate buffer pH ca 4.8 - 6.3
  • Acetate buffer sodium acetate, pH ca 2.5 - 6.
  • Histidine buffer pH ca 6.0 - 7.8
  • Glutamic acid pH 8.0 to 10.2
  • Glycine N, N-bis (2-hydroxyethyl) glycine: pH about 8.6 to 10.6
  • Glycinate buffer pH ca 6.5 - 7.5 lmidazole: pH 6.2 to 7, 8
  • Potassium chloride pH about 1, 0 to 2.2
  • Lactate buffer pH ca 3 .0 - 6.0
  • Tartrate buffer pH ca 3.0 - 5.0
  • Tris pH ca 6.8 - 7.7
  • pH 4-10 preferably pH 5-9
  • the theoretically conceivable temperature range is the temperature of -10 ° C to 40 ° C; preferably 0-25 ° C, more preferably 4 ° C and 20 ° C.
  • 6.1 amino acids e.g. Arginine, omithine, lysine, histidine, glutamic acid, aspartic acid, isoleucine, leucine, alanine, phenylalanine, tyrosine, tryptophan, methionine, serine, proline
  • Sugars and sugar alcohols eg sucrose, lactose, glucose, mannose, maltose, galactose, fructose, sorbose, raffinose, trehalose, glucosamine, N-methyl-glucosamine, galactosamine, neuraminic acid
  • Antioxidants e.g. Acetone sodium bisulfite, ascorbic acid, ascorbic acid ester, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), cysteine, nordihydroguaiaretic acid (NDGA), monothioglycerol, sodium bisulfite, sodium metabisulfite, tocopherols, glutathione
  • Preservative e.g. m-cresol, chlorocresol, phenol, benzyl alcohol, methylparaben, propylparaben, butylparaben, chlorobutanol, phenylmercuric nitrate, phenylmercuric acetate, thimersal, benzalkonium chloride, benzethonium chloride
  • cyclodextrins e.g. Hydroxypropyl- ⁇ -cyclodextrin, sulfobutylethyl- ⁇ -cyclodextrin, y-
  • HSA Human Serum Albumin
  • BSA Bovine Serum Albumin
  • salts e.g. Acetate salts (e.g., sodium acetate), magnesium chloride, calcium chloride, tromethamine, EDTA (e.g., Na-EDTA)
  • sodium chloride potassium chloride
  • glucose glycerol
  • dextrose sodium chloride
  • sodium sulfate sodium chloride, sodium sulfate
  • the addition of the precipitation reagents to the protein solution is carried out in solution ("batch process") .After pipetting, the sample should be mixed by "hand shaking”. The process can be carried out at room temperature or 4 ° C.
  • the addition of the precipitation reagents to the protein solution is carried out in solution ("batch process") .After pipetting, the sample is to be mixed by "hand shaking”. The process can be carried out at room temperature or 4 ° C.
  • the addition of the precipitation reagents to the protein solution is carried out in solution ("batch process") .After pipetting, the sample is to be mixed by "hand shaking”. The procedure can be carried out at room temperature or 4 ° C.
  • the addition of the precipitation reagents to the protein solution is carried out in solution ("batch process") .After pipetting, the sample is to be mixed by "hand shaking”. The process can be carried out at room temperature or 4 ° C.
  • Example 8 Carrying out the method of crystallization described in WO02072636

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Abstract

La présente invention concerne des formes solides d'anticorps anti-récepteur EGF, en particulier des précipités et des cristaux d'anticorps monoclonaux anti-récepteur EGF, de préférence de Mab C225 (Cetuximab) et Mab h425 (EMD 72000), qui conduisent à la dissolution ou à la suspension en milieu aqueux pour donner une protéine d'anticorps à action biologique, et qui peuvent être obtenues par précipitation de l'anticorps qui est dissous ou en suspension dans un milieu aqueux, et/ou d'une variante et/ou d'un fragment de celui-ci, au moyen d'un réactif de précipitation. L'invention a également pour objet des préparations pharmaceutiques contenant au moins une forme solide des anticorps mentionnés ci-dessus dans une forme non cristalline, cristalline précipitée, ou soluble ou en suspension, ainsi qu'éventuellement des substances de support et/ou des substances auxiliaires et/ou d'autres principes actifs pharmaceutiques, ainsi qu'un procédé pour préparer lesdites formes solides d'anticorps anti-EGFR.
EP04797849A 2003-11-29 2004-11-12 Formes solides d'anticorps anti-egfr Ceased EP1686961A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP08011237A EP1974723A3 (fr) 2003-11-29 2004-11-12 Formes solides d'anticorps anti-EGFR

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10355904A DE10355904A1 (de) 2003-11-29 2003-11-29 Feste Formen von anti-EGFR-Antikörpern
PCT/EP2004/012837 WO2005051355A1 (fr) 2003-11-29 2004-11-12 Formes solides d'anticorps anti-egfr

Related Child Applications (1)

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EP08011237A Withdrawn EP1974723A3 (fr) 2003-11-29 2004-11-12 Formes solides d'anticorps anti-EGFR

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EP (2) EP1686961A1 (fr)
KR (2) KR20060111539A (fr)
CN (1) CN1886118A (fr)
AR (1) AR046677A1 (fr)
AU (1) AU2004292742B9 (fr)
BR (1) BRPI0416968A (fr)
CA (1) CA2547446C (fr)
CO (1) CO5690537A2 (fr)
DE (1) DE10355904A1 (fr)
EC (1) ECSP066681A (fr)
IL (1) IL175935A (fr)
MX (1) MXPA06005968A (fr)
MY (1) MY148228A (fr)
NZ (1) NZ548210A (fr)
PE (1) PE20050667A1 (fr)
RU (1) RU2397755C2 (fr)
TW (1) TWI350291B (fr)
UA (1) UA84893C2 (fr)
WO (1) WO2005051355A1 (fr)
ZA (1) ZA200605348B (fr)

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CN1886118A (zh) 2006-12-27
BRPI0416968A (pt) 2007-02-21
CA2547446A1 (fr) 2005-06-09
IL175935A (en) 2013-10-31
EP1974723A3 (fr) 2010-06-09
CA2547446C (fr) 2013-05-14
KR20120093406A (ko) 2012-08-22
MXPA06005968A (es) 2006-07-06
CO5690537A2 (es) 2006-10-31
ZA200605348B (en) 2007-04-25
ECSP066681A (es) 2006-10-25
AU2004292742B2 (en) 2011-08-25
KR20060111539A (ko) 2006-10-27
AU2004292742A1 (en) 2005-06-09
RU2006123005A (ru) 2008-01-20
RU2397755C2 (ru) 2010-08-27
DE10355904A1 (de) 2005-06-30
EP1974723A2 (fr) 2008-10-01
NZ548210A (en) 2010-10-29
MY148228A (en) 2013-03-29
US7960516B2 (en) 2011-06-14
US20070122411A1 (en) 2007-05-31
TWI350291B (en) 2011-10-11
UA84893C2 (en) 2008-12-10
TW200530268A (en) 2005-09-16
IL175935A0 (en) 2006-10-05
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AU2004292742B9 (en) 2011-11-24

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