Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
  • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
  • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
  • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
  • C07H19/16—Purine radicals
  • C07H19/167—Purine radicals with ribosyl as the saccharide radical
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
  • A61P1/04—Drugs for disorders of the alimentary tract or the digestive system for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P11/00—Drugs for disorders of the respiratory system
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P11/00—Drugs for disorders of the respiratory system
  • A61P11/02—Nasal agents, e.g. decongestants
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P11/00—Drugs for disorders of the respiratory system
  • A61P11/06—Antiasthmatics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P11/00—Drugs for disorders of the respiratory system
  • A61P11/08—Bronchodilators
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
  • A61P15/08—Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
  • A61P15/10—Drugs for genital or sexual disorders; Contraceptives for impotence
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
  • A61P15/16—Masculine contraceptives
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P17/00—Drugs for dermatological disorders
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P17/00—Drugs for dermatological disorders
  • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P17/00—Drugs for dermatological disorders
  • A61P17/04—Antipruritics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P17/00—Drugs for dermatological disorders
  • A61P17/06—Antipsoriatics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P19/00—Drugs for skeletal disorders
  • A61P19/02—Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P25/00—Drugs for disorders of the nervous system
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P25/00—Drugs for disorders of the nervous system
  • A61P25/08—Antiepileptics; Anticonvulsants
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P25/00—Drugs for disorders of the nervous system
  • A61P25/18—Antipsychotics, i.e. neuroleptics; Drugs for mania or schizophrenia
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P25/00—Drugs for disorders of the nervous system
  • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P27/00—Drugs for disorders of the senses
  • A61P27/16—Otologicals
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P3/00—Drugs for disorders of the metabolism
  • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
  • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
  • A61P31/04—Antibacterial agents
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P37/00—Drugs for immunological or allergic disorders
  • A61P37/08—Antiallergic agents
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P9/00—Drugs for disorders of the cardiovascular system
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P9/00—Drugs for disorders of the cardiovascular system
  • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P9/00—Drugs for disorders of the cardiovascular system
  • A61P9/12—Antihypertensives

Definitions

• This invention relates to purine derivatives. More particularly, this invention relates to 2-aminocarbonyl-9H-purine derivatives and to processes for the preparation of, intermediates used in the preparation of, compositions containing and the uses of, such derivatives.
• These derivatives are selective, functional agonists of the human adenosine A2a receptor and may be used as anti-inflammatory agents in the treatment of, inter alia, diseases of the respiratory tract.
• Adenosine is a ubiquitous molecule having a central role in mammalian intermediary metabolism. Independently, adenosine acts on multiple surface receptors to produce a variety of responses. Adenosine receptor classification has revealed the presence of at least four subtypes: A1, A2a, A2b and A3. Stimulation of adenosine A2 receptors on the surface of human neutrophils has been reported to potently inhibit a range of neutrophil functions. Activated neutrophils can damage lung tissue by release of reactive oxygen species, for example, superoxide anion radicals (O 2 - ), and granule products, for example, human neutrophil elastase (HNE), amongst other inflammatory mediators.
• reactive oxygen species for example, superoxide anion radicals (O 2 - )
• HNE human neutrophil elastase
• LTB 4 is a potent chemo-attractant that recruits additional neutrophils to the inflammatory focus, whereas released O 2 - and HNE adversely affect the pulmonary extracellular matrix.
• the A2 receptor subtype mediating many of these responses (O 2 - and LTB 4 /HNE release and cell adhesion) is established as A2a.
• the A2 subtype (A2a or A2b) mediating the other effects remains to be established.
• Selective agonist activity at the A2a receptor is considered to offer greater therapeutic benefit than the use of non-selective adenosine receptor agonists because interaction with other subtypes is associated with detrimental effects in the lung in animal models and human tissue studies. For example, asthmatics, but not non-asthmatics, bronchoconstrict when challenged with inhaled adenosine. This response is at least in part due to the activation of the A1 receptor subtype. Activation of A1 receptors also promotes neutrophil chemotaxis and adherence to endothelial cells, thus promoting lung injury.
• Adenine derivatives having adenosine A2a receptor agonist activity are disclosed in WO 00/23457 . They are useful in the treatment of inflammatory diseases.
• the present purine derivatives inhibit neutrophil function and are selective agonists of the adenosine A2a receptor. They may also have antagonist activity at the adenosine A3 receptor.
• the present compounds may be used to treat any disease for which an adenosine A2a receptor agonist is indicated. They can be used to treat a disease where leukocyte (e.g. neutrophil, eosinophil, basophil, lymphocyte, macrophage) - induced tissue damage is implicated.
• leukocyte e.g. neutrophil, eosinophil, basophil, lymphocyte, macrophage
• ARDS adult respiratory distress syndrome
• bronchitis chronic bronchitis
• chronic obstructive pulmonary disease cystic fibrosis
• asthma emphysema
• bronchiectasis chronic sinusitis and rhinitis.
• the present compounds may also be used in the treatment of septic shock, male erectile dysfunction, male factor infertility, female factor infertility, hypertension, stroke, epilepsy, cerebral ischaemia, peripheral vascular disease, post-ischaemic reperfusion injury, diabetes, rheumatoid arthritis, multiple sclerosis, psoriasis, dermatitis, allergic dermatitis, eczema, ulcerative colitis, Crohns disease, inflammatory bowel disease, Heliobacter pylori gastritis, non -Heliobacter pylori gastritis, non-steroidal anti-inflammatory drug-induced damage to the gastro-intestinal tract or a psychotic disorder, or for wound healing.
• the present invention provides a compound of the formula: or a pharmaceutically acceptable salt or solvate thereof, wherein R 1 is H, C 1 -C 6 alkyl or fluorenyl, said C 1 -C 6 alkyl being optionally substituted by 1 or 2 substituents each independently selected from phenyl and naphthyl, said phenyl and naphthyl being optionally substituted by C 1 -C 6 alkyl, C 1 -C 6 alkoxy, halo or cyano;
• the present invention provides a compound of the formula: or a pharmaceutically acceptable salt or solvate thereof, wherein R 1 is H, C 1 -C 6 alkyl or fluorenyl, said C 1 -C 6 alkyl being optionally substituted by 1 or 2 substituents each independently selected from phenyl and naphthyl, said phenyl and naphthyl being optionally substituted by C 1 -C 6 alkyl, C 1 -C 6 alkoxy, halo or cyano; R 2 is H or C 1 -C 6 alkyl; either, R 3 and R 4 , taken together with the nitrogen atom to which they are attached, represent azetidinyl, pyrrolidinyl, piperidinyl, piperazinyl, homopiperidinyl or homopiperazinyl, each being optionally substituted on a ring nitrogen or carbon atom by C 1 -C 6 alkyl or C 3 -C 8 cycloalkyl
• halo means fluoro, chloro, bromo or iodo and alkyl, alkylene, alkanoyl and alkoxy groups containing the requisite number of carbon atoms, except where indicated, can be unbranched or branched chain.
• alkyl include methyl, ethyl, n-propyl, i-propyl, n-butyl, i-butyl, sec-butyl and t-butyl.
• alkoxy include methoxy, ethoxy, n-propoxy, i-propoxy, n-butoxy, i-butoxy, sec-butoxy and t-butoxy.
• alkanoyl examples include acetyl and propanoyl.
• alkylene examples include methylene, 1,1-ethylene, 1,2-ethylene, 1,3-propylene and 1,2-propylene.
• alkylene examples include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl and cycloheptyl (the corresponding examples for cycloalkoxy also apply).
• cycloalkylene include cyclopentylene, cyclohexylene and cycloheptylene.
• Het can be aromatic or partially or fully saturated and "C-linked" means that it is attached to the neighbouring group by a ring carbon atom.
• hetero examples include pyrrolyl, imidazolyl, triazolyl, thienyl, furyl, thiazolyl, oxazolyl, thiadiazolyl, oxadiazolyl, pyridinyl, pyrimidinyl, pyridazinyl and pyrazinyl.
• the pharmaceutically acceptable salts of the compounds of the formula (I) include the acid addition and the base salts thereof.
• Suitable acid addition salts are formed from acids which form non-toxic salts and examples are the hydrochloride, hydrobromide, hydroiodide, sulphate, bisulphate, nitrate, phosphate, hydrogen phosphate, acetate, maleate, malate, fumarate, lactate, tartrate, citrate, gluconate, succinate, saccharate, benzoate, methanesulphonate, ethanesulphonate, benzenesulphonate, p -toluenesulphonate, pamoate, adipate and xinafoate (1-hydroxy-2-naphthoate) salts.
• Suitable base salts are formed from bases which form non-toxic salts and examples are the sodium, potassium, aluminium, calcium, magnesium, zinc and diethanolamine salts.
• the pharmaceutically acceptable solvates of the compounds of the formula (I) and salts thereof include hydrates thereof.
• a compound of the formula (I) may contain one or more additional asymmetric carbon atoms and therefore exist in two or more stereoisomeric forms.
• the present invention includes the individual stereoisomers of the compounds of the formula (I) and, where appropriate, the individual tautomeric forms thereof, together with mixtures thereof.
• Separation of diastereoisomers may be achieved by conventional techniques, e.g. by fractional crystallisation, chromatography or H.P.L.C. of a stereoisomeric mixture of a compound of the formula (I) or a suitable salt or derivative thereof.
• An individual enantiomer of a compound of the formula (I) may also be prepared from a corresponding optically pure intermediate or by resolution, such as by H.P.L.C. of the corresponding racemate using a suitable chiral support or by fractional crystallisation of the diastereoisomeric salts formed by reaction of the corresponding racemate with a suitable optically active acid or base, as appropriate.
• R 1 is C 1 -C 6 alkyl optionally substituted by 1 or 2 phenyl substituents, said phenyl being optionally substituted by C 1 -C 6 alkyl or halo.
• R 1 is C 1 -C 6 alkyl optionally substituted by 1 or 2 phenyl substituents, said phenyl being optionally substituted by methyl or chloro.
• R 1 is C 1 -C 6 alkyl optionally substituted by 1 or 2 phenyl substituents.
• R 1 is C 1 -C 6 alkyl substituted by 1 or 2 phenyl substituents, said phenyl being optionally substituted by methyl or chloro.
• R 1 is C 1 -C 6 alkyl substituted by 1 or 2 phenyl substituents.
• R 1 is C 1 -C 6 alkyl substituted by 2 phenyl substituents, said phenyl being optionally substituted by methyl or chloro.
• R 1 is C 1 -C 6 alkyl substituted by 2 substituents each independently selected from phenyl, 3-methylphenyl and 3-chlorophenyl.
• R 1 is C 1 -C 6 alkyl substituted by 2 phenyl substituents.
• R 1 is diphenylethyl, bis(3-methylphenyl)ethyl or bis(3-chlorophenyl)ethyl.
• R 1 is diphenylethyl.
• R 1 is 2,2-diphenylethyl, 2,2-bis(3-methylphenyl)ethyl or 2,2-bis(3-chlorophenyl)ethyl.
• R 1 is 2,2-diphenylethyl.
• R 2 is H.
• R 15 is H.
• X is 1,2-ethylene or 1,3-propylene.
• X is 1,2-ethylene.
• R 2 is H
• R 15 is H
• X is 1,2-ethylene, 1,3-propylene or a group of the formula: -(CH 2 ) n -W-(CH 2 ) p - where W is C 5 -C 7 cycloalkylene, n is 0 or 1 and p is 0 or 1.
• R 2 is H
• R 15 is H
• X is 1,2-ethylene, 1,3-propylene or a group of the formula: -(CH 2 ) n -W-(CH 2 ) p - where W is C 5 -C 7 cycloalkylene, n is 0 and p is 0.
• R 2 is H
• R 15 is H
• X is 1,2-ethylene, 1,3-propylene or cyclohexylene.
• R 2 is H
• R 15 is H
• X is 1,2-ethylene, 1,3-propylene or 1,4-cyclohexylene.
• R 2 is H
• R 15 is H
• X is 1,2-ethylene, 1,3-propylene or trans-1,4-cyclohexylene.
• R 2 is H
• R 15 is H
• X is 1,2-ethylene
• R 15 is H and R 2 and X, taken together with the nitrogen atom to which they are attached, represent 3-pyrrolidinyl or 3- or 4-piperidinyl, each being optionally substituted by C 1 -C 6 alkyl.
• R 15 is H and R 2 and X, taken together with the nitrogen atom to which they are attached, represent 3-pyrrolidinyl or 4-piperidinyl each being optionally substituted by C 1 -C 6 alkyl.
• R 15 is H and R 2 and X, taken together with the nitrogen atom to which they are attached, represent 3-pyrrolidinyl or 3- or 4-piperidinyl.
• R 15 is H and R 2 and X, taken together with the nitrogen atom to which they are attached, represent 3-pyrrolidinyl or 4-piperidinyl.
• R 15 is H and R 2 and X, taken together with the nitrogen atom to which they are attached, represent (3R)-pyrrolidinyl or 4-piperidinyl.
• R 2 is H and R 15 and X, taken together with the nitrogen atom to which they are attached, represent 3-pyrrolidinyl or 3- or 4-piperidinyl, each being optionally substituted by C 1 -C 6 alkyl.
• R 2 is H and R 15 and X, taken together with the nitrogen atom to which they are attached, represent 3-pyrrolidinyl or 4-piperidinyl each being optionally substituted by C 1 -C 6 alkyl.
• R 2 is H and R 15 and X, taken together with the nitrogen atom to which they are attached, represent 3-pyrrolidinyl or 3- or 4-piperidinyl.
• R 2 is H and R 15 and X, taken together with the nitrogen atom to which they are attached, represent 3-pyrrolidinyl or 4-piperidinyl.
• R 2 is H and R 15 and X, taken together with the nitrogen atom to which they are attached, represent (3R)-pyrrolidinyl, (3S)-pyrrolidinyl or 4-piperidinyl.
• R 3 is H.
• R 4 is piperidin-3-yl or piperidin-4-yl, each optionally substituted by benzyl or het as previously defined.
• R 4 is piperidin-3-yl or piperidin-4-yl, each optionally substituted by benzyl, pyridin-2-yl, pyridin-3-yl or pyridin-4-yl, said pyridin-2-yl, pyridin-3-yl and pyridin-4-yl each optionally substituted by C 1 -C 6 alkyl, C 3 -C 8 cycloalkyl, C 1 -C 6 alkoxy, C 3 -C 8 cycloalkoxy, hydroxy, oxo or halo.
• R 4 is piperidin-3-yl or piperidin-4-yl, each substituted by benzyl, pyridin-2-yl, pyridin-3-yl or pyridin-4-yl.
• R 4 is piperidin-3-yl or piperidin-4-yl, each substituted by benzyl.
• R 4 is piperidin-3-yl or piperidin-4-yl, each substituted by pyridin-2-yl.
• R 4 is piperidin-4-yl substituted by pyridin-2-yl.
• R 4 is 1-benzylpiperidin-4-yl.
• R 4 is 1-(pyridin-2-yl)piperidin-4-yl.
• R 4 is -(C 2 -C 6 alkylene)-R 8 .
• R 4 is -CH 2 CH 2 R 8 .
• R 4 is -(C 1 -C 6 alkylene)-R 13 .
• R 4 is -CH 2 R 13 or -CH 2 CH 2 R 13 .
• R 4 is C 3 -C 8 cycloalkyl.
• R 4 is cyclohexyl
• R 5 is -CH 2 OH or -CONH(C 1 -C 6 alkyl).
• R 5 is -CH 2 OH or -CONHCH 2 CH 3 .
• R 5 is -CONHCH 2 CH 3 .
• R 8 is (i) azetidin-1-yl, pyrrolidin-1-yl, piperidin-1-yl, morpholin-4-yl, piperazin-1-yl, homopiperidin-1-yl, homopiperazin-1-yl or tetrahydroisoquinolin-1-yl, each being optionally substituted on a ring carbon atom by C 1 -C 6 alkyl and said piperazin-1-yl and homopiperazin-1-yl being optionally substituted on the ring nitrogen atom not attached to the C 2 -C 6 alkylene group by C 1 -C 6 alkyl, or (ii) is NR 11 R 12 .
• R 8 is piperidin-1-yl or tetrahydroisoquinolin-1-yl each optionally substituted on a ring carbon atom by C 1 -C 6 alkyl.
• R 8 is piperidin-1-yl optionally substituted on a ring carbon atom by isopropyl.
• R 8 is piperidin-1-yl, 4-isopropylpiperidin-1-yl or tetrahydroisoquinolin-1-yl.
• R 8 is NR 11 R 12 where NR 11 R 12 is N(C 1 -C 6 alkyl) 2 , N(C 1 -C 6 alkyl)(C 3 -C 8 cycloalkyl) or N(C 1 -C 6 alkyl)(benzyl).
• R 8 is NR 11 R 12 where NR 11 R 12 is N,N-diisopropylamino, N,N-din-butylamino, N-cyclopentyl-N-isopropylamino, N-cyclohexyl-N-isopropylamino or N-benzyl-N-isopropylamino.
• R 11 is H or C 1 -C 6 alkyl.
• R 11 is C 1 -C 6 alkyl.
• R 11 is isopropyl or n-butyl.
• R 12 is H, C 1 -C 6 alkyl, C 3 -C 8 cycloalkyl or benzyl.
• R 12 is C 1 -C 6 alkyl, C 3 -C 8 cycloalkyl or benzyl.
• R 12 is isopropyl, cyclopentyl, cyclohexyl or benzyl.
• R 13 is either phenyl optionally substituted by -(C 1 -C 3 alkylene)-NR 14 R 14 or -CO 2 H, or piperidin-2-yl, piperidin-3-yl or piperidin-4-yl each optionally substituted by benzyl.
• R 13 is phenyl optionally substituted by -CH 2 N(CH 2 CH 3 ) 2 or - CO 2 H, or piperidin-4-yl substituted by benzyl.
• R 13 is phenyl, 4-(N,N-diethylamino)methylphenyl, 4-carboxyphenyl or 1-benzylpiperidin-4-yl.
• R 14 is H or C 1 -C 6 alkyl.
• R 14 is H or ethyl.
• Y is CO.
• Et means ethyl
• iPr means isopropyl
• nBu means n-butyl
• Ph means phenyl
• Particularly preferred embodiments of a compound of the formula (I) are those of the Examples section hereafter, particularly those of Examples 8 and 34, together with pharmaceutically acceptable salts and solvates thereof.
• the compounds of the formula (I) can be prepared using conventional procedures such as by the following illustrative methods in which R 1 , R 2 , R 3 , R 4 , R 5 , R 15 , X and Y are as previously defined for a compound of the formula (I) unless otherwise stated.
• a pharmaceutically acceptable salt of a compound of the formula (I) may be readily prepared by mixing together solutions of a compound of the formula (I) and the desired acid or base, as appropriate.
• the salt may precipitate from solution and be collected by filtration or may be recovered by evaporation of the solvent.
• the anti-inflammatory properties of the compounds of the formula (I) are demonstrated by their ability to inhibit neutrophil function which indicates A2a receptor agonist activity. This is evaluated by determining the compound profile in an assay where superoxide production was measured from neutrophils activated by fMLP. Neutrophils were isolated from human peripheral blood using dextran sedimentation followed by centrifugation through Ficoll-Hypaque solution. Any contaminating erythrocytes in the granulocyte pellet were removed by lysis with ice-cold distilled water. Superoxide production from the neutrophils was induced by fMLP in the presence of a priming concentration of cytochalasin B.
• Adenosine deaminase was included in the assay to remove any endogenously produced adenosine that might suppress superoxide production.
• the effect of the compound on the fMLP-induced response was monitored colorometrically from the reduction of cytochrome C within the assay buffer.
• the potency of the compounds was assessed by the concentration giving 50% inhibition (IC 50 ) compared to the control response to fMLP.
• the compounds of the formula (I) can be administered alone but will generally be administered in admixture with a suitable pharmaceutical excipient, diluent or carrier selected with regard to the intended route of administration and standard pharmaceutical practice.
• the compounds of the formula (I) can be administered orally, buccally or sublingually in the form of tablets, capsules, multi-particulates, gels, films, ovules, elixirs, solutions or suspensions, which may contain flavouring or colouring agents, for immediate-, delayed-, modified-, sustained-, pulsed- or controlled-release applications.
• the compounds of the formula (I) may also be administered as fast-dispersing or fast-dissolving dosage forms or in the form of a high energy dispersion or as coated particles. Suitable formulations of the compounds of the formula (I) may be in coated or uncoated form, as desired.
• Such solid pharmaceutical compositions for example, tablets may contain excipients such as microcrystalline cellulose, lactose, sodium citrate, calcium carbonate, dibasic calcium phosphate, glycine and starch (preferably corn, potato or tapioca starch), disintegrants such as sodium starch glycollate, croscarmellose sodium and certain complex silicates, and granulation binders such as polyvinylpyrrolidone, hydroxypropylmethylcellulose (HPMC), hydroxypropylcellulose (HPC), sucrose, gelatin and acacia.
• lubricating agents such as magnesium stearate, sodium stearyl fumarate, sodium lauryl sulphate, stearic acid, glyceryl behenate and talc may be included.
• a formulation of the tablet could typically contain from 0.01 mg to 500mg of active compound whilst tablet fill weights may range from 50mg to 1000mg.
• An example of a formulation for a 10mg tablet is illustrated below: Ingredient %w/w Compound of the formula (I) or salt 10.000* Lactose 64.125 Starch 21.375 Croscarmellose sodium 3.00 Magnesium Stearate 1.500 * Quantity adjusted in accordance with drug activity.
• the tablets can be manufactured by a standard process, for example, direct compression or a wet or dry granulation process.
• the tablet cores may be coated with appropriate overcoats.
• Solid compositions of a similar type may also be employed as fillers in gelatin or HPMC capsules.
• Preferred excipients in this regard include lactose, starch, a cellulose, milk sugar or a high molecular weight polyethylene glycol.
• the compounds of the formula (I) may be combined with various sweetening or flavouring agents, colouring matter or dyes, with emulsifying and/or suspending agents and with diluents such as water, ethanol, propylene glycol or glycerin, and combinations thereof.
• the compounds of the formula (I) can also be administered parenterally, for example, intravenously, intra-arterially, intraperitoneally, intrathecally, intraventricularly, intraurethrally, intrasternally, intracranially, intramuscularly or subcutaneously, or they may be administered by infusion or needleless injection techniques.
• parenteral administration they are best used in the form of a sterile aqueous solution which may contain other substances, for example, a co-solvent and/or enough salts or glucose to make the solution isotonic with blood.
• the aqueous solutions should be suitably buffered (preferably to a pH of from 3 to 9), if necessary.
• the preparation of suitable parenteral formulations under sterile conditions is readily accomplished by standard pharmaceutical techniques well-known to those skilled in the art.
• the daily dosage level of the compounds of the formula (I) will usually be from 0.00001 to 100 mg/kg, preferably from 0.0001 to 100 mg/kg (in single or divided doses).
• tablets or capsules of the compound of the formula (I) may contain from 0.01 to 500 mg of active compound for administration singly or two or more at a time, as appropriate.
• the physician in any event will determine the actual dosage which will be most suitable for any individual patient and it will vary with the age, weight and response of the particular patient.
• the above dosages are exemplary of the average case. There can, of course, be individual instances where higher or lower dosage ranges are merited and such are within the scope of this invention.
• the compounds of formula (I) can also be administered intranasally or by inhalation and are conveniently delivered in the form of a dry powder inhaler or an aerosol spray presentation from a pressurised container, pump, spray, atomiser (preferably an atomiser using electrohydrodynamics to produce a fine mist) or nebuliser, with or without the use of a suitable propellant, e.g.
• the dosage unit may be determined by providing a valve to deliver a metered amount.
• the pressurised container, pump, spray, atomiser or nebuliser may contain a solution or suspension of the active compound, e.g. using a mixture of ethanol (optionally, aqueous ethanol) or a suitable agent for dispersing, solubilising or extending release and the propellant as the solvent, which may additionally contain a lubricant, e.g. sorbitan trioleate.
• a lubricant e.g. sorbitan trioleate.
• Capsules, blisters and cartridges for use in an inhaler or insufflator may be formulated to contain a powder mix of a compound of the formula (I), a suitable powder base such as lactose or starch and a performance modifier such as I-leucine, mannitol or magnesium stearate.
• a suitable solution formulation for use in an atomiser using electrohydrodynamics to produce a fine mist may contain from 1 ⁇ g to 10mg of a compound of the formula (I) or a salt thereof and the actuation volume may vary from 1 to 100 ⁇ L.
• a typical formulation may comprise a compound of the formula (I) or salt thereof, propylene glycol, sterile water, ethanol and sodium chloride.
• Aerosol or dry powder formulations are preferably arranged so that each metered dose or "puff" contains from 1 to 4000 ⁇ g of a compound of the formula (I) for delivery to the patient.
• the overall daily dose with an aerosol will be in the range of from 1 ⁇ g to 20mg which may be administered in a single dose or, more usually, in divided doses throughout the day.
• the compounds of the formula (I) can be administered in the form of a suppository or pessary, or they may be applied topically in the form of a lotion, solution, cream, ointment or dusting powder.
• the compounds of the formula (I) may also be dermally or transdermally administered, for example, by the use of a skin patch. They may also be administered by the pulmonary, vaginal or rectal routes.
• the compounds of the formula (I) can be formulated as a suitable ointment containing the active compound suspended or dissolved in, for example, a mixture with one or more of the following: mineral oil, liquid petrolatum, white petrolatum, propylene glycol, polyoxyethylene polyoxypropylene compound, emulsifying wax and water.
• they can be formulated as a suitable lotion or cream, suspended or dissolved in, for example, a mixture of one or more of the following: mineral oil, sorbitan monostearate, a polyethylene glycol, liquid paraffin, polysorbate 60, cetyl esters wax, cetearyl alcohol, 2-octyldodecanol, benzyl alcohol and water.
• the compounds of the formula (I) may also be used in combination with a cyclodextrin.
• Cyclodextrins are known to form inclusion and non-inclusion complexes with drug molecules. Formation of a drug-cyclodextrin complex may modify the solubility, dissolution rate, bioavailability and/or stability property of a drug molecule. Drug-cyclodextrin complexes are generally useful for most dosage forms and administration routes.
• the cyclodextrin may be used as an auxiliary additive, e.g. as a carrier, diluent or solubiliser.
• Alpha-, beta- and gamma-cyclodextrins are most commonly used and suitable examples are described in WO-A-91/11172 , WO-A-94/02518 and WO-A-98/55148 .
• THF tetrahydrofuran
• DMSO dimethylsulphoxide
• TLC thin layer chromatography
• Table 1 shows the compound structures and Table 2 the analytical data for each compound.
• n-Bu in Table 1 means n-butyl.
• the imidazolide starting material for Examples 13, 24 and 25 may be prepared as described in Monatsh. Chem., 88, 35 (1957 ).
• the imidazolide starting material for Example 14 and 26 may be prepared as described in J. Chem. Soc. Perkin Trans. 1, 11, 1205 (1996 ).
• Example 15 The imidazolide starting material for Example 15 may be prepared as described in Justus Liebigs Ann. Chem., 648, 72 (1961 ). TABLE 1 Example No. Compound 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 TABLE 2 Example No.
• the reaction mixture was allowed to cool to ambient temperature and saturated aqueous sodium bicarbonate solution (200 ml) was cautiously added. The resultant mixture was then concentrated in vacuo and the aqueous mixture was then extracted with ethyl acetate (200 ml) and then dichloromethane (200 ml). The extracts were dried over anhydrous magnesium sulphate which caused the deposition of a gum that was redissolved by the addition of dichloromethane (100 ml) and methanol (20 ml).
• the resultant violet solution was then concentrated in vacuo to give the crude product as a purple foam (20.86 g) that was purified by flash chromatography on silica gel (600 g) eluting with a gradient of 6% v/v methanol in dichloromethane changing to 8% v/v methanol in dichloromethane changing to 10% v/v methanol in dichloromethane changing to 15% v/v methanol in dichloromethane to give the title compound in several fractions of varying purity.
• the major fraction (11.4 g) was dissolved in dichloromethane (264 ml) and was filtered to remove insoluble matter.
• 2,6-Dichloro-9 H- purine (20 g, 0.11 mol) and 4-toluenesulphonic acid monohydrate (0.2 g) were dissolved in ethyl acetate (300 ml), the mixture heated to 50°C and a solution of 3,4-dihydro-2H-pyran (12.6 ml, 0.14 mol) in ethyl acetate (50 ml) added slowly over 30 minutes.
• the reaction mixture was cooled to room temperature, water (100 ml) added and the pH of the solution adjusted to 7 by addition of a saturated aqueous solution of sodium hydrogen carbonate.
• Oxalyl chloride (14.0 ml, 160 mmol) was added dropwise to a stirred solution of (3a R, 4 S, 6 R, 6a R )-6-methoxy-2,2-dimethyltetrahydrofuro[3,4 -d ][1,3]dioxole-4-carboxylic acid ( J. Amer. Chem. Soc., 80, 5168-5173 (1958 )) (23.30 g, 107 mmol) in anhydrous dichloromethane (120 ml) and N,N-dimethylformamide (2 drops) and the mixture stirred at room temperature for 3 hours until gas evolution had ceased.
• N 1 ,N 1 -Diisopropyl-1,2-ethanediamine (1 g, 6.94 mmol) was added to a stirred solution of N,N'-carbonyldiimidazole (1.12 g, 6.94 mmol) in dichloromethane (50 ml) at room temperature.
• the reaction mixture was stirred for one hour and diluted with dichloromethane (50 ml), washed with water (60 ml), dried (anhydrous magnesium sulfate) and the solvent removed under reduced pressure. This gave the title compound as a white solid (600 mg).
• Lithium aluminium hydride (66ml of a 1 molar solution in tetrahydrofuran, 0.066mol) was added to a stirred solution of [cyclopentyl(isopropyl)amino]acetonitrile (10g, 0.66mol) (Preparation 24) in tetrahydrofuran (100ml) at 0°C.
• the reaction mixture was stirred at 0°C for 20 minutes and then heated under reflux for 2 hours.
• the reaction mixture was allowed to cool to room temperature and left to stand overnight.
• the reaction mixture was cooled in an icebath and treated dropwise with 4.8ml of a 7.5% w/w aqueous sodium hydroxide solution followed by 7.4 ml of water.
• the mixture was dissolved in a little dichloromethane and purified by column chromatography on silica gel eluting with a gradient system of dichloromethane : methanol : concentrated aqueous ammonia (80 : 20 : 1.2, by volume) changing to dichloromethane : methanol : concentrated aqueous ammonia (88 : 12 : 2, by volume). After evaporation of appropriate fractions the residue was triturated with diethyl ether, filtered and dried to yield the target compound as a white solid, (0.22 g, 58 %).
• the mixture was dissolved in a little dichloromethane and purified by column chromatography on silica gel eluting with a gradient system of dichloromethane : methanol : concentrated aqueous ammonia (80 : 20 : 1.2, by volume) changing to dichloromethane : methanol : concentrated aqueous ammonia (88 : 12 : 2, by volume). After evaporation of appropriate fractions the residue was triturated with diethyl ether, filtered and dried to yield the target compound as a white solid, (0.32 g, 79 %).
• the mixture was dissolved in a little dichloromethane and purified by column chromatography on silica gel eluting with a gradient system of dichloromethane : methanol (98 : 2, by volume) changing to dichloromethane : methanol (95 : 5, by volume). After evaporation of appropriate fractions the residue was triturated with diethyl ether, filtered and dried to yield the target compound as a white solid, (1.0 g, 80 %).
• the catalyst was removed by filtration through Arbocel (trade mark), solvent removed by evaporation under reduced pressure and the residue purified by column chromatography on silica gel eluting with a gradient system of dichloromethane : methanol : concentrated aqueous ammonia (90 : 10 : 1, by volume) changing to dichloromethane : methanol : concentrated aqueous ammonia (80 : 20 : 2, by volume). After evaporation of appropriate fractions the target compound was obtained as a white solid, (0.6 g, 67 %).
• the residue was purified by column chromatography on silica gel eluting with a gradient system of dichloromethane : methanol : concentrated aqueous ammonia (95 : 5 : 0.5, by volume) changing to dichloromethane : methanol : concentrated aqueous ammonia (90 : 10 : 2, by volume). After evaporation of appropriate fractions the target compound was obtained as a white solid, (0.98 g).
• n-Butyl nitrite (4.65 ml, 39.7 mmol) was added to a suspension of (2 R ,3 R ,4 S ,5 S )-2-(2-amino-6-chloro-9 H -purin-9-yl)-4-(benzoyloxy)-5-[(ethylamino)carbonyl]-tetrahydro-3-furanyl benzoate (Preparation 46) (8.10 g, 14.7 mmol), iodine (3.73 g, 14.7 mmol), copper(I) iodide (6.16 g, 32.3 mmol) and diiodomethane (12.55 ml, 155.8 mmol) in THF (100 ml) and the mixture was heated under reflux for 2.5 hours.
• Benzyl 4-(aminomethyl)benzoate hydrochloride (1.0 g, 36 mmol) was dissolved in 10 % w/w aqueous sodium hydroxide solution (20 ml) and the solution extracted with dichloromethane (30 ml). The organic phase was separated, dried over anhydrous magnesium sulfate and solvent evaporated under reduced pressure to give the free base of the amine as a thick oil. This was dissolved in dichloromethane (20 ml) and the solution added dropwise to a solution of N,N'-carbonyldiimidazole (1.62 g, 10 mmol) in dichloromethane (20 ml).
• the mixture was then heated at 103-107°C with stirring for 10 hours, and the autoclave was then vented, flushed with carbon monoxide, and then re-pressurised to 2000kPa with carbon monoxide. Heating at 103-107°C with stirring was continued for a further 14 hours. The mixture was cooled to 60°C and then filtered through a bed of warm Celite (trade mark).
• the resultant orange solution was then heated to 55°C, and a solution of 1,2,3,5-tetra-O-acetyl- ⁇ -D-ribofuranose (36.1 g, 0.113 moles) in 1,2-dimethoxyethane (100 ml plus 20 ml to rinse through) was added over a period of 10 minutes.
• the reaction was then stirred at 57-60°C for 2 hours after which time the mixture was cooled to ambient temperature.
• the reaction mixture was then cautiously added to a mixture of saturated aqueous sodium bicarbonate solution (400 ml) and ethyl acetate (400 ml) with vigorous stirring. The layers were separated and the aqueous phase was extracted with ethyl acetate (400 ml).
• the mixture was concentrated in vacuo to give the crude product as a light brown foam (63.7 g) that was used as such for the next step.
• the crude product can be purified by flash chromatography on silica gel eluting with a gradient system of 5% v/v isopropanol in dichloromethane changing to 7.5% v/v isopropanol in dichloromethane changing to 10% v/v isopropanol in dichloromethane followed by crystallisation from tertiary -butyl methyl ether to give the title compound as colourless crystals, m.p. 118-120°C.
• LRMS positive atmospheric pressure chemical ionisation
• reaction mixture was then added to saturated aqueous sodium bicarbonate solution (300 ml) and the resultant mixture was stirred at ambient temperature for 0.2 hours.
• the mixture was concentrated in vacuo and the aqueous residue was extracted with ethyl acetate (400 ml then 200 ml).
• the organic phases were combined and washed with saturated brine (300 ml), dried over anhydrous magnesium sulphate and concentrated in vacuo to give a yellow foam (58.2 g).
• a solution of this material in tertiary-butyl methyl ether (250 ml) was stirred at ambient temperature, under an atmosphere of nitrogen, to give a suspension that was then cooled in ice for 1 hour and the solid was then collected by filtration.
• the filter cake was washed with cold tertiary -butyl methyl ether (50 ml) and the product was dried in vacuo to give the title compound as a colourless solid (12.7 g).
• the mother liquors were purified by flash chromatography on silica gel (400 g) eluting with 75% v/v ethyl acetate in heptane to give slightly impure product (32.9g) as a foam that was crystallised from tertiary-butyl methyl ether (150 ml) to give more title compound (8.3 g).
• the resultant mixture was heated to 45°C with stirring.
• a solution of sodium chlorite (6.5 g, 0.072 moles) in water (75 ml) and a solution of sodium hypochlorite (0.32 ml of commercial solution with 12% w/v available chlorine, 0.000551 moles) in water (38 ml) were added separately and simultaneously to the stirred mixture over a period of 1 hour.
• the resultant mixture was then stirred at 45-50°C for 10 hours, and for 18 hours at ambient temperature.
• the reaction mixture was then added to a solution of sodium sulphite (18g, 0.143 moles) in water (300 ml) with stirring.
• the layers were separated and the aqueous phase was extracted with ethyl acetate (50 ml).
• the organic phases were combined and were washed successively with deionised water (200 ml), saturated aqueous sodium bicarbonate solution (200 ml) and saturated brine (200 ml), and were subsequently dried over anhydrous magnesium sulphate and concentrated in vacuo to give the crude product as a cream coloured foam (20.22 g).
• the mixture was washed with aqueous citric acid (150 ml of a 0.5M solution) that had been saturated with sodium chloride.
• the layers were separated, and the aqueous phase was extracted with dichloromethane (75 ml).
• the organic phases were combined and were washed with saturated aqueous sodium bicarbonate solution. After separating the phases, the aqueous layer was extracted with dichloromethane (75 ml) and the organic phases were then combined, dried over anhydrous magnesium sulphate and then were concentrated in vacuo to give the title compound (21.28 g) as a light blue foam that was used as such for the next step.
• This crude product can be purified by standard means such as by flash chromatography on silica gel eluting with a system comprising 95:5:0.5, by volume, dichloromethane:methanol:concentrated aqueous ammonia to give the pure title compound.
• LRMS positive atmospheric pressure chemical ionisation: m/z [MH + ] 818.
• the resultant light brown solution was stirred at ambient temperature for 20 minutes and a solution of tert -butyl N-(2-aminoethyl)carbamate (14.0 g, 0.0874 moles) in acetonitrile (10 ml plus 5 ml rinse) was added over a period of 5 minutes. The resultant mixture was then heated under reflux for 2.5 hours. After cooling to ambient temperature, the reaction mixture was concentrated in vacuo and the residue was dissolved in ethyl acetate (150 ml). This solution was washed successively with saturated aqueous sodium bicarbonate solution (70 ml) and deionised water (20 ml).
• the aqueous phases were extracted with ethyl acetate (2 x 100 ml) and the combined organic phases were washed with water.
• the organic phase was dried over anhydrous magnesium sulphate and the solution was concentrated to a volume of approximately 200 ml in vacuo.
• the resultant solution was then distilled at atmospheric pressure until the volume was approximately 75 ml.
• the solution was allowed to cool to ambient temperature during which time crystallisation occurred.
• the resultant thick slurry was diluted with ethyl acetate (60 ml) and was cooled in ice. The solid was collected by filtration and the filter cake was washed with cold ethyl acetate (2 x 30 ml).
• the reaction mixture was stirred for a further 2 hours and it was then added to a solution of 1,2-diaminoethane (2.4 g, 2.7 ml, 0,04 moles) in ethyl acetate (3 ml) over 15 minutes together with the additional ethyl acetate (17 ml) used to rinse through the apparatus.
• the resultant mixture was stirred at ambient temperature for 18 hours and was washed with saturated aqueous sodium bicarbonate solution. The layers were then separated and the aqueous phase was extracted with ethyl acetate (2 x 20 ml). The organic layers were then combined, dried over anhydrous magnesium sulphate and were then concentrated in vacuo to give the crude product (0.83 g).
• Examples 1-35 were tested for biological activity by the method described on pages 51 and 52 and all were found to have an IC 50 of less than 100nM.

Landscapes

• Health & Medical Sciences (AREA)
• Chemical & Material Sciences (AREA)
• Organic Chemistry (AREA)
• Engineering & Computer Science (AREA)
• General Health & Medical Sciences (AREA)
• Life Sciences & Earth Sciences (AREA)
• Bioinformatics & Cheminformatics (AREA)
• Pharmacology & Pharmacy (AREA)
• Public Health (AREA)
• General Chemical & Material Sciences (AREA)
• Medicinal Chemistry (AREA)
• Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
• Veterinary Medicine (AREA)
• Chemical Kinetics & Catalysis (AREA)
• Animal Behavior & Ethology (AREA)
• Pulmonology (AREA)
• Neurology (AREA)
• Biomedical Technology (AREA)
• Neurosurgery (AREA)
• Dermatology (AREA)
• Reproductive Health (AREA)
• Endocrinology (AREA)
• Diabetes (AREA)
• Cardiology (AREA)
• Heart & Thoracic Surgery (AREA)
• Biochemistry (AREA)
• Biotechnology (AREA)
• Gynecology & Obstetrics (AREA)
• Immunology (AREA)
• Rheumatology (AREA)
• Molecular Biology (AREA)
• Genetics & Genomics (AREA)
• Psychiatry (AREA)
• Pain & Pain Management (AREA)
• Communicable Diseases (AREA)
• Hospice & Palliative Care (AREA)
• Orthopedic Medicine & Surgery (AREA)
• Physical Education & Sports Medicine (AREA)
• Oncology (AREA)
• Obesity (AREA)

Applications Claiming Priority (7)

Publications (2)

Family

ID=27255754

Family Applications (1)

Country Status (34)

Families Citing this family (51)

Family Cites Families (3)

• 2001
  • 2001-05-31 TW TW090113146A patent/TWI227240B/zh not_active IP Right Cessation
  • 2001-06-04 AR ARP010102656A patent/AR030289A1/es active IP Right Grant
  • 2001-06-05 GC GCP20011415 patent/GC0000287A/en active
  • 2001-06-05 AU AU2001260537A patent/AU2001260537B2/en not_active Ceased
  • 2001-06-05 DZ DZ013439A patent/DZ3439A1/fr active
  • 2001-06-05 BR BR0111263-5A patent/BR0111263A/pt not_active IP Right Cessation
  • 2001-06-05 JP JP2002501916A patent/JP4129176B2/ja not_active Expired - Fee Related
  • 2001-06-05 MX MXPA02012033A patent/MXPA02012033A/es active IP Right Grant
  • 2001-06-05 IL IL15278301A patent/IL152783A0/xx active IP Right Grant
  • 2001-06-05 DK DK01934242T patent/DK1292604T3/da active
  • 2001-06-05 ES ES01934242T patent/ES2324816T3/es not_active Expired - Lifetime
  • 2001-06-05 CZ CZ20023875A patent/CZ20023875A3/cs unknown
  • 2001-06-05 OA OA1200200363A patent/OA12279A/en unknown
  • 2001-06-05 HU HU0301330A patent/HUP0301330A3/hu unknown
  • 2001-06-05 EA EA200201179A patent/EA004983B1/ru not_active IP Right Cessation
  • 2001-06-05 EP EP01934242A patent/EP1292604B1/en not_active Expired - Lifetime
  • 2001-06-05 PL PL36043501A patent/PL360435A1/xx not_active Application Discontinuation
  • 2001-06-05 KR KR10-2002-7016606A patent/KR100484704B1/ko not_active IP Right Cessation
  • 2001-06-05 CA CA002414018A patent/CA2414018C/en not_active Expired - Fee Related
  • 2001-06-05 AU AU6053701A patent/AU6053701A/xx active Pending
  • 2001-06-05 AT AT01934242T patent/ATE431356T1/de not_active IP Right Cessation
  • 2001-06-05 CN CNB018108024A patent/CN1249074C/zh not_active Expired - Fee Related
  • 2001-06-05 EE EEP200200678A patent/EE200200678A/xx unknown
  • 2001-06-05 SK SK1675-2002A patent/SK16752002A3/sk unknown
  • 2001-06-05 DE DE60138702T patent/DE60138702D1/de not_active Expired - Lifetime
  • 2001-06-05 NZ NZ522184A patent/NZ522184A/en unknown
  • 2001-06-05 WO PCT/IB2001/000973 patent/WO2001094368A1/en active Application Filing
  • 2001-06-06 PA PA20018518801A patent/PA8518801A1/es unknown
• 2002
  • 2002-10-23 BG BG107216A patent/BG107216A/bg unknown
  • 2002-10-29 IS IS6599A patent/IS6599A/is unknown
  • 2002-11-26 CU CU20020281A patent/CU23240B7/es not_active IP Right Cessation
  • 2002-12-02 MA MA26930A patent/MA26910A1/fr unknown
  • 2002-12-04 NO NO20025821A patent/NO324426B1/no not_active IP Right Cessation
  • 2002-12-05 HR HR20020960A patent/HRP20020960A2/hr not_active Application Discontinuation
• 2003
  • 2003-09-05 HK HK03106312A patent/HK1054042A1/xx not_active IP Right Cessation
• 2006
  • 2006-09-05 HK HK06109837A patent/HK1089447A1/xx not_active IP Right Cessation
• 2007
  • 2007-06-21 IL IL184144A patent/IL184144A/en not_active IP Right Cessation
• 2008
  • 2008-01-15 JP JP2008005804A patent/JP2008150383A/ja active Pending

Also Published As

Similar Documents

Legal Events