EA201691990A1 - Получение библиотек вариантов белков, экспрессированных в эукариотических клетках, и применение их для отбора связывающих молекул - Google Patents

Получение библиотек вариантов белков, экспрессированных в эукариотических клетках, и применение их для отбора связывающих молекул

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EA201691990A1
EA201691990A1 EA201691990A EA201691990A EA201691990A1 EA 201691990 A1 EA201691990 A1 EA 201691990A1 EA 201691990 A EA201691990 A EA 201691990A EA 201691990 A EA201691990 A EA 201691990A EA 201691990 A1 EA201691990 A1 EA 201691990A1
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binding molecules
library
options
selection
obtaining
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EA201691990A
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EA034190B1 (ru
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Джон Маккафферти
Майкл Дайсон
Котай Партибан
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Ионтас Лимитед
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    • C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins
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Abstract

Настоящее изобретение относится к способам получения библиотек эукариотических клеток, кодирующих набор связывающих молекул ("связывающие молекулы"), причем в указанных способах используется сайт-специфическая нуклеаза для направленного расщепления клеточной ДНК с целью улучшения сайт-специфического встраивания генов связывающих молекул посредством эндогенных клеточных механизмов репарации. Получают популяции эукариотических клеток, в которых набор генов, кодирующих связывающие молекулы, встроен в требуемый локус в клеточной ДНК (например, геномный локус), что обеспечивает экспрессию кодируемой связывающей молекулы, в результате чего получают популяцию клеток, экспрессирующих разные связывающие молекулы.
EA201691990A 2014-05-02 2015-05-01 Получение библиотек вариантов белков, экспрессированных в эукариотических клетках, и применение их для отбора связывающих молекул EA034190B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1407852.1A GB201407852D0 (en) 2014-05-02 2014-05-02 Preparation of libraries od protein variants expressed in eukaryotic cells and use for selecting binding molecules
PCT/GB2015/051287 WO2015166272A2 (en) 2014-05-02 2015-05-01 Preparation of libraries of protein variants expressed in eukaryotic cells and use for selecting binding molecules

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EA201691990A1 true EA201691990A1 (ru) 2017-05-31
EA034190B1 EA034190B1 (ru) 2020-01-15

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EA201691990A EA034190B1 (ru) 2014-05-02 2015-05-01 Получение библиотек вариантов белков, экспрессированных в эукариотических клетках, и применение их для отбора связывающих молекул

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US (4) US11286477B2 (ru)
EP (3) EP3461893B1 (ru)
JP (3) JP6440824B2 (ru)
CN (1) CN106574264A (ru)
AU (1) AU2015255013B2 (ru)
BR (1) BR112016025352B1 (ru)
CA (1) CA2947513A1 (ru)
DK (2) DK3461893T3 (ru)
EA (1) EA034190B1 (ru)
ES (1) ES2712632T3 (ru)
GB (1) GB201407852D0 (ru)
HR (1) HRP20190187T1 (ru)
PT (2) PT3137602T (ru)
SG (1) SG11201609123RA (ru)
WO (1) WO2015166272A2 (ru)

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