EA037825B1 - ВЫСОКОТОЧНЫЕ ВАРИАНТЫ Cas9 И ИХ ПРИМЕНЕНИЯ - Google Patents

ВЫСОКОТОЧНЫЕ ВАРИАНТЫ Cas9 И ИХ ПРИМЕНЕНИЯ Download PDF

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Publication number
EA037825B1
EA037825B1 EA201991913A EA201991913A EA037825B1 EA 037825 B1 EA037825 B1 EA 037825B1 EA 201991913 A EA201991913 A EA 201991913A EA 201991913 A EA201991913 A EA 201991913A EA 037825 B1 EA037825 B1 EA 037825B1
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Eurasian Patent Office
Prior art keywords
mutations
cas9
seq
target
spcas9
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EA201991913A
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English (en)
Russian (ru)
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EA201991913A1 (ru
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Анна Черезето
Антонио Казини
Джанлука Петрис
Альберто Инга
Микеле Оливьери
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Университа` Дельи Студи Ди Тренто
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Publication of EA201991913A1 publication Critical patent/EA201991913A1/ru
Publication of EA037825B1 publication Critical patent/EA037825B1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/71—Fusion polypeptide containing domain for protein-protein interaction containing domain for transcriptional activaation, e.g. VP16
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
EA201991913A 2017-02-14 2018-02-14 ВЫСОКОТОЧНЫЕ ВАРИАНТЫ Cas9 И ИХ ПРИМЕНЕНИЯ EA037825B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IT102017000016321A IT201700016321A1 (it) 2017-02-14 2017-02-14 Mutanti di cas9 ad alta specificita' e loro applicazioni.
PCT/EP2018/053717 WO2018149888A1 (en) 2017-02-14 2018-02-14 High-fidelity cas9 variants and applications thereof

Publications (2)

Publication Number Publication Date
EA201991913A1 EA201991913A1 (ru) 2020-01-22
EA037825B1 true EA037825B1 (ru) 2021-05-25

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EA201991913A EA037825B1 (ru) 2017-02-14 2018-02-14 ВЫСОКОТОЧНЫЕ ВАРИАНТЫ Cas9 И ИХ ПРИМЕНЕНИЯ

Country Status (12)

Country Link
US (1) US11525127B2 (https=)
EP (1) EP3583209B8 (https=)
JP (1) JP7232531B2 (https=)
KR (1) KR20190116407A (https=)
CN (1) CN110520528B (https=)
AU (1) AU2018221644A1 (https=)
BR (1) BR112019016765A2 (https=)
CA (1) CA3053218A1 (https=)
EA (1) EA037825B1 (https=)
IT (1) IT201700016321A1 (https=)
MX (1) MX2019009651A (https=)
WO (1) WO2018149888A1 (https=)

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Also Published As

Publication number Publication date
JP7232531B2 (ja) 2023-03-03
CN110520528B (zh) 2024-08-13
EP3583209B8 (en) 2025-02-26
WO2018149888A1 (en) 2018-08-23
MX2019009651A (es) 2019-12-18
CA3053218A1 (en) 2018-08-23
US11525127B2 (en) 2022-12-13
EP3583209C0 (en) 2024-12-04
IT201700016321A1 (it) 2018-08-14
EP3583209A1 (en) 2019-12-25
BR112019016765A2 (pt) 2020-05-26
EP3583209B1 (en) 2024-12-04
EA201991913A1 (ru) 2020-01-22
AU2018221644A1 (en) 2019-08-29
JP2020507347A (ja) 2020-03-12
KR20190116407A (ko) 2019-10-14
CN110520528A (zh) 2019-11-29
US20200149020A1 (en) 2020-05-14

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