BR112019016765A2 - Molécula de cas9 modificada isolada, método para produzir uma cas9 modificada, complexos de proteína ou ribonucleoproteína ou complexos de proteína e ribonucleoproteína misturadas ou lipídeo, proteína de fusão, sequência de nucleotídeos, ácido nucleico, vetor, célula, animal ou planta, composição farmacêutica, uso in vitro de uma cas9 modificada recombinante, kit de partes, e, método in vitro para alterar o genoma de uma célula - Google Patents
Molécula de cas9 modificada isolada, método para produzir uma cas9 modificada, complexos de proteína ou ribonucleoproteína ou complexos de proteína e ribonucleoproteína misturadas ou lipídeo, proteína de fusão, sequência de nucleotídeos, ácido nucleico, vetor, célula, animal ou planta, composição farmacêutica, uso in vitro de uma cas9 modificada recombinante, kit de partes, e, método in vitro para alterar o genoma de uma célula Download PDFInfo
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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- C07K—PEPTIDES
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
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- C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT102017000016321 | 2017-02-14 | ||
| IT102017000016321A IT201700016321A1 (it) | 2017-02-14 | 2017-02-14 | Mutanti di cas9 ad alta specificita' e loro applicazioni. |
| PCT/EP2018/053717 WO2018149888A1 (en) | 2017-02-14 | 2018-02-14 | High-fidelity cas9 variants and applications thereof |
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| BR112019016765A2 true BR112019016765A2 (pt) | 2020-05-26 |
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| CN112626070A (zh) * | 2021-01-13 | 2021-04-09 | 海南微氪生物科技股份有限公司 | 一种基于切离酶基因编辑技术的定向修复系统 |
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| CN120225668A (zh) * | 2023-10-25 | 2025-06-27 | 石药集团中奇制药技术（石家庄）有限公司 | 一种高效低脱靶的基因编辑工具 |
| CN117887760A (zh) * | 2024-01-22 | 2024-04-16 | 中国医学科学院北京协和医院 | SQSTM1/p62基因新致病突变c.1143delC的敲入小鼠模型及其构建方法 |
| CN118497204B (zh) * | 2024-07-16 | 2024-12-03 | 西北农林科技大学深圳研究院 | 一种质粒CRISPR-pCas9n及基因编辑的方法与应用 |
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| EP3004339B1 (en) * | 2013-05-29 | 2021-07-07 | Cellectis | New compact scaffold of cas9 in the type ii crispr system |
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| US11214779B2 (en) * | 2015-04-08 | 2022-01-04 | University of Pittsburgh—of the Commonwealth System of Higher Education | Activatable CRISPR/CAS9 for spatial and temporal control of genome editing |
| TWI906646B (zh) * | 2015-06-18 | 2025-12-01 | 美商博得學院股份有限公司 | 降低脫靶效應的ｃｒｉｓｐｒ酶突變 |
| US9512446B1 (en) * | 2015-08-28 | 2016-12-06 | The General Hospital Corporation | Engineered CRISPR-Cas9 nucleases |
| IT201700016321A1 (it) | 2017-02-14 | 2018-08-14 | Univ Degli Studi Di Trento | Mutanti di cas9 ad alta specificita' e loro applicazioni. |
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| JP7232531B2 (ja) | 2023-03-03 |
| EA037825B1 (ru) | 2021-05-25 |
| CN110520528B (zh) | 2024-08-13 |
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| WO2018149888A1 (en) | 2018-08-23 |
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| CA3053218A1 (en) | 2018-08-23 |
| US11525127B2 (en) | 2022-12-13 |
| EP3583209C0 (en) | 2024-12-04 |
| IT201700016321A1 (it) | 2018-08-14 |
| EP3583209A1 (en) | 2019-12-25 |
| EP3583209B1 (en) | 2024-12-04 |
| EA201991913A1 (ru) | 2020-01-22 |
| AU2018221644A1 (en) | 2019-08-29 |
| JP2020507347A (ja) | 2020-03-12 |
| KR20190116407A (ko) | 2019-10-14 |
| CN110520528A (zh) | 2019-11-29 |
| US20200149020A1 (en) | 2020-05-14 |
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