EA028250B1 - Новая молочнокислая бактерия, обладающая сверхтекстурирующим свойством с мутациями в гене galk, и способы ее получения - Google Patents

Новая молочнокислая бактерия, обладающая сверхтекстурирующим свойством с мутациями в гене galk, и способы ее получения Download PDF

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Publication number
EA028250B1
EA028250B1 EA201270351A EA201270351A EA028250B1 EA 028250 B1 EA028250 B1 EA 028250B1 EA 201270351 A EA201270351 A EA 201270351A EA 201270351 A EA201270351 A EA 201270351A EA 028250 B1 EA028250 B1 EA 028250B1
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EA
Eurasian Patent Office
Prior art keywords
strain
lactic acid
species
acid bacterium
milk
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Application number
EA201270351A
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English (en)
Russian (ru)
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EA201270351A1 (ru
Inventor
Томас Янцен
Дитте Эллегор Кристиансен
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Кр. Хансен А/С
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Application filed by Кр. Хансен А/С filed Critical Кр. Хансен А/С
Publication of EA201270351A1 publication Critical patent/EA201270351A1/ru
Publication of EA028250B1 publication Critical patent/EA028250B1/ru

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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
    • A23C9/00Milk preparations; Milk powder or milk powder preparations
    • A23C9/12Fermented milk preparations; Treatment using microorganisms or enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • C12N1/205Bacterial isolates
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
    • A23C19/00Cheese; Cheese preparations; Making thereof
    • A23C19/02Making cheese curd
    • A23C19/032Making cheese curd characterised by the use of specific microorganisms, or enzymes of microbial origin
    • A23C19/0323Making cheese curd characterised by the use of specific microorganisms, or enzymes of microbial origin using only lactic acid bacteria, e.g. Pediococcus and Leuconostoc species; Bifidobacteria; Microbial starters in general
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
    • A23C9/00Milk preparations; Milk powder or milk powder preparations
    • A23C9/12Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/123Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
    • A23C9/1238Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt using specific L. bulgaricus or S. thermophilus microorganisms; using entrapped or encapsulated yoghurt bacteria; Physical or chemical treatment of L. bulgaricus or S. thermophilus cultures; Fermentation only with L. bulgaricus or only with S. thermophilus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L29/00Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/20Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
    • A23L29/269Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of microbial origin, e.g. xanthan or dextran
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135Bacteria or derivatives thereof, e.g. probiotics
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y207/00Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01006Galactokinase (2.7.1.6)
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
    • A23C2220/00Biochemical treatment
    • A23C2220/20Treatment with microorganisms
    • A23C2220/206Slime forming bacteria; Exopolysaccharide or thickener producing bacteria, ropy cultures, so-called filant strains
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/21Streptococcus, lactococcus
    • A23V2400/249Thermophilus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/46Streptococcus ; Enterococcus; Lactococcus

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Dispersion Chemistry (AREA)
  • Dairy Products (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
EA201270351A 2009-09-01 2010-09-01 Новая молочнокислая бактерия, обладающая сверхтекстурирующим свойством с мутациями в гене galk, и способы ее получения EA028250B1 (ru)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DKPA200900984 2009-09-01
DKPA200900984 2009-09-01
DKPA201000070 2010-01-28
DKPA201000070 2010-01-28
PCT/EP2010/062808 WO2011026863A1 (en) 2009-09-01 2010-09-01 Lactic bacterium with modified galactokinase expression for texturizing food products by overexpression of exopolysaccharide

Publications (2)

Publication Number Publication Date
EA201270351A1 EA201270351A1 (ru) 2012-08-30
EA028250B1 true EA028250B1 (ru) 2017-10-31

Family

ID=42829610

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201270351A EA028250B1 (ru) 2009-09-01 2010-09-01 Новая молочнокислая бактерия, обладающая сверхтекстурирующим свойством с мутациями в гене galk, и способы ее получения

Country Status (12)

Country Link
US (2) US20120164275A1 (enExample)
EP (1) EP2473058B2 (enExample)
JP (1) JP5944824B2 (enExample)
KR (1) KR101773994B1 (enExample)
CN (1) CN102695420B (enExample)
BR (1) BR112012004706B1 (enExample)
DK (1) DK2473058T4 (enExample)
EA (1) EA028250B1 (enExample)
ES (1) ES2644220T5 (enExample)
MX (1) MX344042B (enExample)
PL (1) PL2473058T5 (enExample)
WO (1) WO2011026863A1 (enExample)

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TR201807378T4 (tr) 2010-01-28 2018-06-21 Chr Hansen As Faj dayanımına göre seçilen gıda ürünlerinin tekstüre edilmesi için laktik bakteriler.
EP2630265B1 (en) 2010-10-22 2017-03-22 Chr. Hansen A/S Texturizing lactic acid bacteria strains
CN102559705A (zh) * 2010-10-28 2012-07-11 内蒙古蒙牛乳业(集团)股份有限公司 一种EPS基因簇EpsA、EpsB、EpsC、EpsD
MX367899B (es) * 2012-04-25 2019-09-11 Chr Hansen As Uso de bacterias de acido lactico para preparar productos alimenticios fermentados con mayor dulzura natural.
EP2796050A1 (en) * 2013-04-24 2014-10-29 Danone GmbH Lactic acid bacteria capable of enhancing fruit flavour
WO2015074054A1 (en) * 2013-11-18 2015-05-21 The Trustees Of Columbia University In The City Of New York Improving microbial fitness in the mammalian gut
WO2015193449A1 (en) * 2014-06-19 2015-12-23 Chr. Hansen A/S Method of producing a fermented milk product with reduced residual lactose concentration
CN108777970A (zh) * 2015-12-18 2018-11-09 科·汉森有限公司 用于制备具有增强的天然甜度和高质地的发酵食品的乳酸菌
WO2017167660A1 (en) 2016-03-31 2017-10-05 Chr. Hansen A/S Use of glucose deficient streptococcus thermophiles strains in a process for producing fermented milk products
BR112019003112A2 (pt) * 2016-09-01 2019-07-09 Chr Hansen As bactérias
WO2018177835A1 (en) 2017-03-28 2018-10-04 Chr. Hansen A/S Lactic acid bacteria composition for preparing fermented food products with increased natural sweetness and flavor
JP6940305B2 (ja) 2017-06-01 2021-09-22 株式会社明治 チーズ風味素材の製造方法
CN111433347B (zh) * 2017-08-28 2023-09-08 科·汉森有限公司 用于制备例如马苏里拉干酪的嗜热链球菌(st)细胞
EA202090441A1 (ru) 2017-08-30 2020-07-07 Кхр. Хансен А/С Способ получения улучшенного мезофильного ферментированного молочного продукта
WO2019061263A1 (en) * 2017-09-29 2019-04-04 Dupont Nutrition Biosciences Aps NOVEL STRAIN OF LACTOBACILLUS PLANTARUM AND USES THEREOF
CN111527196A (zh) 2017-12-22 2020-08-11 杜邦营养生物科学有限公司 具有增甜特性的新型乳酸细菌及其用途
MX2020013816A (es) 2018-06-20 2021-03-09 Chr Hansen As Metodo para producir queso con una cantidad reducida de galactosa.
WO2020038931A1 (en) 2018-08-21 2020-02-27 Chr. Hansen A/S Process for producing an improved fermented milk product using a sporulation negative bacillus strain
BR112021012116A2 (pt) 2018-12-21 2021-09-08 Dupont Nutrition Biosciences Aps Bactérias formadoras de ácido láctico
CN109735556A (zh) * 2019-02-22 2019-05-10 昆明理工大学 引导糖基转移酶基因的用途
US20220312785A1 (en) 2019-06-20 2022-10-06 Chr. Hansen A/S Use of st gal(+) bacteria for producing a fermented milk product with a relatively high stable ph
PL3845069T3 (pl) 2019-12-30 2023-01-30 Compagnie Gervais Danone Ujemny względem sacharozy streptococcus thermophilus do zastosowania do wytwarzania wyrobów fermentowanych
JP2023528345A (ja) 2020-05-29 2023-07-04 セーホーエル.ハンセン アクティーゼルスカブ 発酵食品製品を調製するための乳酸菌組成物
US20230416673A1 (en) * 2020-11-17 2023-12-28 Chr. Hansen A/S Lactic acid bacterial strain with improved texturizing properties
WO2022180071A2 (en) 2021-02-26 2022-09-01 Chr. Hansen A/S Lactic acid bacteria composition for preparing fermented products
EP4112715A1 (en) 2021-07-01 2023-01-04 Compagnie Gervais Danone Bifidobacteria for use in preparation of fermented products
WO2025132763A1 (en) 2023-12-22 2025-06-26 Chr. Hansen A/S Glucose-hyperfermenting lactic acid bacteria
CN120866265A (zh) * 2025-09-26 2025-10-31 杭州瑞宏生物技术有限公司 一种提高β-果糖基转移酶活力的工艺

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Publication number Priority date Publication date Assignee Title
US20060240539A1 (en) * 2003-03-17 2006-10-26 Philippe Horvath Texturizing lactic bacteria
WO2007095958A1 (en) * 2006-02-24 2007-08-30 Chr. Hansen A/S Lactic acid bacteria providing improved texture of fermented dairy products

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BROADBENT J R ET AL: "Biochemistry, genetics, and applications of exopolysaccharide production in Streptococcus thermophilus: a review.", JOURNAL OF DAIRY SCIENCE., AMERICAN DAIRY SCIENCE ASSOCIATION, US, vol. 86, no. 2, 1 February 2003 (2003-02-01), US, pages 407 - 423, XP009018436, ISSN: 0022-0302, DOI: 10.3168/jds.S0022-0302(03)73619-4 *
DE VIN FILIP ET AL: "Molecular and biochemical analysis of the galactose phenotype of dairy Streptococcus thermophilus strains reveals four different fermentation profiles", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 71, no. 7, 1 July 2005 (2005-07-01), pages 3659 - 3667, XP002604686, ISSN: 0099-2240, DOI: 10.1128/aem.71.7.3659-3667.2005 *
HASSAN A N, ET AL: "Microstructure and rheology of yogurt made with cultures differing only in their ability to produce exopolysaccharides.", JOURNAL OF DAIRY SCIENCE., AMERICAN DAIRY SCIENCE ASSOCIATION, US, vol. 86, no. 5, 1 May 2003 (2003-05-01), US, pages 1632 - 1638, XP002377643, ISSN: 0022-0302, DOI: 10.3168/jds.S0022-0302(03)73748-5 *
MOZZI F ET AL: "Effect of galactose and glucose on the exopolysaccharide production and the activities of biosynthetic enzymes in Lactobacillus casei CRL 87", JOURNAL OF APPLIED MICROBIOLOGY., WILEY-BLACKWELL PUBLISHING LTD., GB, vol. 91, no. 1, 1 July 2001 (2001-07-01), GB, pages 160 - 167, XP002604687, ISSN: 1364-5072 *
ROBITAILLE, G. ; MOINEAU, S. ; ST-GELAIS, D. ; VADEBONCOEUR, C. ; BRITTEN, M.: "Galactose Metabolism and Capsule Formation in a Recombinant Strain of Streptococcus thermophilus with a Galactose-Fermenting Phenotype", JOURNAL OF DAIRY SCIENCE., AMERICAN DAIRY SCIENCE ASSOCIATION, US, vol. 90, no. 9, 1 September 2007 (2007-09-01), US, pages 4051 - 4057, XP026956143, ISSN: 0022-0302 *
ROBITAILLE, G. ; TREMBLAY, A. ; MOINEAU, S. ; ST-GELAIS, D. ; VADEBONCOEUR, C. ; BRITTEN, M.: "Fat-free yogurt made using a galactose-positive exopolysaccharide-producing recombinant strain of Streptococcus thermophilus", JOURNAL OF DAIRY SCIENCE., AMERICAN DAIRY SCIENCE ASSOCIATION, US, vol. 92, no. 2, 1 February 2009 (2009-02-01), US, pages 477 - 482, XP026955226, ISSN: 0022-0302 *
VAILLANCOURT KATY ET AL: "Characterization of a galactokinase-positive recombinant strain of Streptococcus thermophilus.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 70, no. 8, 1 August 2004 (2004-08-01), pages 4596 - 4603, XP002604685, ISSN: 0099-2240, DOI: 10.1128/aem.70.8.4596-4603.2004 *

Also Published As

Publication number Publication date
DK2473058T4 (da) 2025-02-17
US20120164275A1 (en) 2012-06-28
CN102695420A (zh) 2012-09-26
BR112012004706A2 (pt) 2015-09-08
ES2644220T5 (en) 2025-04-21
MX2012002594A (es) 2012-04-19
KR101773994B1 (ko) 2017-09-01
DK2473058T3 (en) 2017-09-18
WO2011026863A1 (en) 2011-03-10
EP2473058A1 (en) 2012-07-11
EA201270351A1 (ru) 2012-08-30
ES2644220T3 (es) 2017-11-28
CN102695420B (zh) 2016-01-20
EP2473058B2 (en) 2024-12-25
US20170298457A1 (en) 2017-10-19
JP5944824B2 (ja) 2016-07-05
MX344042B (es) 2016-12-02
KR20120073238A (ko) 2012-07-04
JP2013503604A (ja) 2013-02-04
PL2473058T5 (pl) 2025-04-28
BR112012004706B1 (pt) 2019-09-10
PL2473058T3 (pl) 2018-02-28
EP2473058B1 (en) 2017-08-09

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